Basics Of Operation - Hitachi L-2100 Instruction Manual

4.2.1 Basics of Operation

When the settings are all finished, carry out operation in the
following procedure.
(1) Make sure the pump, column, detector and data
processor are properly connected.
(2) Set the mobile phase and waste solution bottle in place.
(3) Start the pump and wait until the flow path is filled with
the mobile phase.
(4) Warm up the pump, column and detector and wait until
the baseline stabilizes.
(5) Inject a sample.
(6) When analysis is finished, turn the power off and dispose
of the waste solution.
NOTICE: Before connecting a new column, make sure the flow
path is filled with mobile phase so that air won't enter the
column.
NOTICE: The whirring sound of the pump during operation
varies depending on such operating conditions as flow rate and
pressure. Although the whirring sound may become larger
anywhere in the high-pressure range, it is not a symptom of
abnormality.
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