Low-Pressure Gradient System - Hitachi L-2100 Instruction Manual

1.2 Low-Pressure Gradient System

In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
Washing solution
Column
Mixer
Degasser
(1) Each of plural eluents is fed from the degasser to the low-
pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-
specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
1 - 3
Eluent
System power supply unit
Model L-2400 UV detector
Model L-2300 column oven
Model L-2200 autosampler
Low-pressure
gradient unit
Model L-2100/2130 pump
Drain
1.2