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4.4 Selection of Mobile Phase and Cautions on Handling
(1)
Be very careful about the restrictions on use with respect to
characteristics and temperature of the mobile phase to be
used. (See Table 3-4 Characteristics of Organic Solvents in
3. SYSTEM OPERATION)
(2)
For high-sensitivity analysis with the diode array detector,
use a solvent having a very low UV absorption
characteristic. A solvent for liquid chromatography that is
available on the market should be used. With generally
used high-grade reagents such as methanol, ethanol or
acetonitrile, a considerable difference occurs in absorption
characteristic at short wavelengths, and this varies with the
production lot and manufacturer. Caution is required for
high-sensitivity analysis.
(3)
When the solvent having a low UV absorption (e.g.
acetonitrile or water) is used as the mobile phase, the life
time of the flow cell may become shorter because of the
degradation and the decrease of the transmittance of
quartz capillary of the flow cell due to UV exposure.
We recommend you to turn off the light source lamp after
the completion of analysis because the degradation will
become slow if lamp light irradiation time decreases. For
the setting of the automatic light extinction function after
the completion of analysis, refer to the section of the
method setting in the instruction manual of CDS.
(4)
Concerning the Restrictions on the Mobile Phase
(a)
Since the wetted material of the flow path of the flow
cell use PEEK resin, there may be restrictions on
solvents or reagents that could corrode or swell
PEEK resin such as tetrahydrofuran, THF
dichloromethane, chloroform, acetone,
dimethylsulfoxide (DMSO). Using these solvents may
causes to clog the flow path of the flow cell.
(b)
Since the wetted material of the flow path of the flow
cell use stainless steel (SUS316), there may be
restrictions on solvents or reagents that could corrode
stainless steel. Especially, solvents or solutions that
contain halide ions such as HCl, KCl, NaCl and
NH
Cl are typical reagents.
4
Reagents that may be restricted are given in Table 4-1; you
may use this table as a rough standard.
4 - 3
4.4
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