cytiva UniFlux 30 Operating Instructions Manual

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UniFlux 30 and UniFlux 120

Operating Instructions

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Page 1 UniFlux 30 and UniFlux 120 Operating Instructions Original instructions cytiva.com...
Page 2: Table Of Contents
Flow ..................................81 3.7.3 pH, conductivity and UV ..........................82 3.7.4 Temperature ..............................83 3.7.5 More information ............................84 Flowcharts ................................ 85 3.8.1 External connections ..........................86 3.8.2 Flow diagrams ..............................88 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 3 Reference information ..................178 Specifications ..............................179 Signals ................................184 Battery limits ..............................186 Chemical resistance ............................. 188 Recycling information ..........................190 Regulatory information ..........................191 8.6.1 Contact information ............................ 192 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 4 Phase 3: Fed Batch Concentration ......................262 Saving the method ............................273 Appendix D: PID control and feedback tuning ..........274 Introduction ..............................275 Using PID control ............................277 More information ............................278 Index ........................... 280 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 5: Introduction
UniFlux™ 30 and UniFlux 120 systems and a list of associated documentation. In this chapter Section See page About this manual Important user information Associated documentation Abbreviations UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 6: About This Manual
Scope of this manual This Operating Instructions covers all variants of UniFlux 30 and UniFlux 120 systems. The components included in the configuration are described in the General Specifica- tion (GS). Classification information for your system is described on the system label.
Page 7: Important User Information
UniFlux 30 and UniFlux 120 systems shall not be used in any clinical procedures, or for diagnostic purposes. UniFlux 30 and UniFlux 120 systems shall not be used for operation in a potentially explosive atmosphere or for handling flammable liquids.
Page 8 1.2 Important user information Prerequisites In order to operate UniFlux 30 and UniFlux 120 systems in a safe way, and according to the intended purpose, the following prerequisites must be met: • You should be acquainted with the use of bioprocessing equipment and with the handling of biological materials.
Page 9: Associated Documentation
In addition to the Operating Instructions, the documentation package supplied with UniFlux 30 and UniFlux 120 systems also includes product documentation binders containing detailed specifications and traceability documents. The most important documents in the document package with regard to technical aspects of UniFlux 30 and UniFlux 120 systems are: Document...
Page 10 1 Introduction 1.3 Associated documentation Component documentation Documentation for components produced both by Cytiva and by a third-party are, if existent, also included in the product documentation package. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 11: Abbreviations
(CV). inputs (CV). Pressure sensor Pressure sensor Pressure regulator Pressure regulator Uninterruptible power supply Uninterruptible power supply UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 12: Safety Instructions
2 Safety instructions Safety instructions About this chapter This chapter describes safety precautions, emergency shutdown procedures and labels information for the UniFlux 30 and UniFlux 120 systems. In this chapter Section See page Safety precautions Labels Emergency procedures Important WARNING...
Page 13: Safety Precautions
WARNING Do not operate the product in any other way than described in the user documentation. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 14 CAUTION Do not insert your fingers or other objects into fans or other moving parts. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 15 WARNING High pressure. The product operates under high pressure. Wear protective glasses and other required Personal Protective Equip- ment (PPE) at all times. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 16 2 Safety instructions 2.1 Safety precautions Installing and moving WARNING The product must be installed and prepared by Cytiva personnel or a third party authorized by Cytiva. WARNING Move transport crates. Make sure that the lifting equipment has the capacity to safely lift the crate weight. Make sure that the crate is properly balanced so that it will not accidentally tip when moved.
Page 17 CAUTION The wheels of the UniFlux 30 and UniFlux 120 systems should be locked during normal use. The wheels should be unlocked only when moving the unit.
Page 18 CAUTION Use the supplied network cable with encased RJ45 connectors to protect from liquids. Do not replace this cable with an unprotected cable. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 19 Installation must comply with specifications regarding wire types, conductor sizes, plugs, branch circuit protection and disconnect devices. Non-compliance may result in personal injury and/or equipment damage. WARNING All electrical installations must be performed by authorized personnel only. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 20 WARNING Never exceed the operating limits stated in this document and on the system label. Operation of the product outside these limits can damage equipment and cause personal injury or death. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 21 Knowledge of how to use UNICORN is required to safely operate the product. Refer to UNICORN user documentation as required. CAUTION When handling the operator console, make sure that no body parts are caught between the sections of the console arm. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 22 Maintenance WARNING Decontaminate before service. Before performing any service work on the system, make sure that the system has been properly decontaminated. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 23 WARNING Use only approved parts. Only spare parts and accessories that are approved or supplied by Cytiva may be used for maintaining or servicing the product. WARNING For continued protection against injury risks due to fluid jets, burst pipes or potentially explosive atmosphere, the user must test the piping system for leakage at maximum operating pressure.
Page 24 Do not climb on any parts of the instrument except where clearly allowed. Follow local regulations and make sure that equipment is properly secured when inspecting the product at high level. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 25: Labels
2 Safety instructions 2.2 Labels Labels Introduction This section describes the various labels on the UniFlux 30 and UniFlux 120 systems and their meaning. System label The system label is located on the back of the equipment. The system label identifies the equipment and shows electrical data, regulatory compliance, and warning symbols.
Page 26 Warning! High Voltage Always make sure that the system is disconnected from electric power before opening the cabinet doors or disconnecting any electric device. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 27 Cabinet 30 208V 238V 241V EMERGENCY STOP label, yellow with black text. (emergency stop button is red). Refer to Section 2.3 Emergency procedures, on page for further information regarding the emergency stop. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 28 Attention: cleaning only when USB cover is closed! Attention: Note: cleaning only when USB cover This label is located beside the USB port on the side is closed! C0035304-01 panel of the monitor. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 29: Emergency Procedures
STOP button should only be performed if an emergency situation prevents normal shutdown. This section describes how to perform an emergency shutdown of the UniFlux 30 and UniFlux 120 systems, the result in the event of power failure, and the procedure for restarting the UniFlux 30 and UniFlux 120 systems after a power failure.
Page 30 • All valves will shut immediately and not in sequence. NOTICE To power down the system, always follow the controlled shutdown procedure described in Section 5.4 Procedures after usage, on page 147. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 31 If an external Uninterruptible Power Supply (UPS) is used, this unit must be powered before any other equipment. Step Action Make sure that the condition that caused the power failure or emergency stop is corrected. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 32 Reset the EMERGENCY STOP button by twisting it clockwise. Press the Continue button in UNICORN. NOTICE If UniFlux system fails to restart correctly following an emergency shutdown or power failure, contact Cytiva for further advice and assistance. ® ® Note:...
Page 33: System Description
3 System description System description About this chapter This chapter provides descriptions of UniFlux 30 and UniFlux 120 systems and an over- view of all the components, including the UNICORN control system. In this chapter Section See page Configurations Illustrations...
Page 34: Configurations
UniFlux 30 and UniFlux 120 systems can be individually configured according to the specific process requirements. This section summarizes the standard and optional components of UniFlux, and indicates where further information on these components is to be found.
Page 35: Illustrations
Illustrations Introduction This section provides illustrations of UniFlux system. The main features and compo- nents are indicated. UniFlux: Front view The illustration below shows a front view of UniFlux 30. Part Function More information Mounting plates for peristaltic pumps Section 3.4.2 Peri-...
Page 36 More information Skid maneouvering handles Hydraulic manouvering unit for filter cassette holder Adjustable floor supports UniFlux: Rear view The illustration below shows a rear view of UniFlux 30. Part Function Connectors on top Electrical cabinet Permeate outlet connection Permeate recycle connection...
Page 37 Connection to transfer pump option P-203 Connection to permeate pump option PROFIBUS Connection to UniFlux tank PROFIBUS™ control (or other controlled external equipment) CAN BUS TANK Connection to UniFlux tank CAN-bus control UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 38 3 System description 3.2 Illustrations UniFlux 30 and 120: External rear wall connectors The illustrations below show the external top connectors on UniFlux 30 and UniFlux 120 systems . Connector Function ETHERNET LAN/WAN Ethernet connection (only present with the optional built-in computer)
Page 39: Standard Components
3 System description 3.3 Standard components Standard components About this section This section provides an overview of the standard components of UniFlux 30 and UniFlux 120 systems. In this section Section See page 3.3.1 Structural components 3.3.2 Control system components 3.3.3...
Page 40: Structural Components
The structure is designed for handling in a production environ- ment and is easy to move and keep clean. The structure occupies a small box-shaped space that makes it easy to fit into any loca- tion in the production facility. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 41 3.3 Standard components 3.3.1 Structural components Electrical cabinet The electrical cabinet contains all electrical and pneumatic equipment. When the door is closed, the entire cabinet is ingress protected according to IP55. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 42: Control System Components
UniFlux system. The CU-960 control unit is located inside the electrical cabinet. Communications Communication with most controlled components mounted outside the electrical cabinet uses the PROFIBUS industry standard communication protocol and hardware. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 43: Connections And Drains
Permeate outlet PERMEATE RECYCLE Permeate recycle to feed tank FEED DRAIN Feed drain point and product outlet Clean-In-Place agent outlet RETENTATE DRAIN Retentate line drain point PERMEATE DRAIN Permeate line drain point UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 44 For a detailed description of interface points between the UniFlux System and the customer plant process equipment, refer to Section 3.8 Flowcharts, on page 85 Section 8.3 Battery limits, on page 186. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 45: Feed Pump And Feed Line
3 System description 3.3 Standard components 3.3.4 Feed pump and feed line 3.3.4 Feed pump and feed line Feed: UniFlux 30 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 46 The internal diameter of the feed line is selected to keep the flow velocity of the product within a favourable range with regard to the flow range for the each system. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 47 3.3.4 Feed pump and feed line Feed pump P-201 The feed pump P-201 installed on UniFlux 30 and UniFlux 120 has a heat shield mounted for user protection (delimited by orange dash line in the illustration below). The heat shield needs to be removed before pump maintenance is performed. For details see Section 6.1 User maintenance schedule, on page...
Page 48: Retentate Line
TMP is controlled by the pressure control valve PCV-341 regulated by UNICORN. The entire retentate line can be shut off by closing the retentate valve XV-051. The retentate line is drained through the drain valve XV-052. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 49: Permeate Line
TMP is controlled by means of the pressure control valve PCV-341 on the retentate line and the permeate pump P-203 if installed (option). Flow control Permeate flow can be controlled by the optional permeate pump, see Permeate control pump P-203, on page UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 50 3.3.6 Permeate line Recycling The permeate recycle line allows permeate to be routed back to the feed tank, for instance when the process is set up and calibrated or during CIP. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 51: Air Integrity Test Device
The air integrity test is described, see Air integrity test on page 137. Characteristic flowrate It is recommended to perform an air integrity test on each new filter to establish its characteristic air or nitrogen flow. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 52: Optional Components
3 System description 3.4 Optional components Optional components About this section UniFlux 30 and UniFlux 120 can be ordered or upgraded with a range of optional components. This section briefly describes the optional components. In this section Section See page 3.4.1...
Page 53: Computer And User Console
60950-1, and be installed and used according to the manufactur- er's instructions. User console The optional console consists of touch screen and keyboard ergonomically designed for use in a clean production environment. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 54: Peristaltic Pumps
As material is separated out from the feed as permeate, the transfer pump can keep the feed tank level within set limits. If a feed tank is not provided by Cytiva, a signal from the customer plant installations can be used to control the transfer pump through UNICORN. Refer to the SW-docu- mentation provided with the system or contact your Cytiva representative for more information.
Page 55: Multiple Inlets
Multiple inlets Illustration Description The feed inlet (option) is an upgrade to a multiple inlets blocks with four valves. Typically, the inlets can be connected to product, buffer, water and CIP. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 56: Uv Monitor
The UV light and detector operates at 280 nm. Since UV measuring employs optics, it must be understood that the optical system must be kept clean and undamaged to provide reliable readings. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 57: Ph Sensor
3.4 Optional components 3.4.5 pH sensor 3.4.5 pH sensor Illustration AE-121 Description The pH sensor AE-121 (option) is installed on the permeate line and is used to measure the pH of the permeate. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 58: Conductivity Sensor
3.4 Optional components 3.4.6 Conductivity sensor 3.4.6 Conductivity sensor Illustration CE-101 Description The conductivity sensor CE-101 (option) is installed on the permeate line and is used to measure the conductivity of the permeate. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 59: Feed Tank
3.4.7 Feed tank Description A series of tanks are available as option for the UniFlux 30 and 120 systems. The tanks are totally integrated with the UNICORN control system to enable fully auto- matic operation. Refer to UniFlux feed tank Operating Instructions, Cytiva article no 28983063, UniFlux System and Tank datafile, Cytiva article no 18117725 or your Cytiva representative for more information regarding UniFlux feed tanks.
Page 60: Filter Dummies
Usage can be for tests and setups where running the system is required while actual filtration is not. Refer to CIP cassette for UniFlux 30, 120, 400, Cytiva article no 28933498 or your local Cytiva representative for more information regarding filter dummies.
Page 61: Hose Kit
Illustration of hose kit Description To connect the UniFlux feed tank to a corresponding UniFlux 30 or 120 system, a set of hoses is required. This hose kit is available as an option from Cytiva. The hoses are made of reinforced PT-cured silicone and are designed to meet or exceed the UniFlux tank and system specifications.
Page 62: Filter Cassette Configuration
The retentate line must be located in the lower position. The UniFlux 30 and UniFlux 120 systems are also equipped with hydraulically operated cassette holders and the entire hydraulic system also needs to be installed.
Page 63: Filter Cassette Components
3 System description 3.5 Filter cassette configuration 3.5.1 Filter cassette components 3.5.1 Filter cassette components Illustration: UniFlux 30 filter cassette configuration UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 64 3 System description 3.5 Filter cassette configuration 3.5.1 Filter cassette components Illustration: UniFlux 120 filter cassette configuration UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 65: Cassette Holder
More information regarding the cassette holders is also available in the UniFlux system Data File with article no 18117765. UniFlux cassette holder UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 66 Cassette holder guide pin Cassette holder movable pressurization plate Cassette holder fixed pressurization plate Hydraulic cylinder Pressurize button Depressurize button Hydraulic unit control box Hydraulic unit operation enabling button Hydraulic unit pressure indicator UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 67: Filter Cassettes
When more than one cassette is used for a filtering run, the holes of the cassettes align with each other to form common sealed channels. Available types of cassettes Refer to your Cytiva representative for information regarding different cassettes that can be used with UniFlux filtration systems. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 68: Hollow Fiber (Hf) Configuration
The retentate line must be located in the upper position. In this section Section See page 3.6.1 UniFlux 30 HF configuration 3.6.2 UniFlux 120 HF configuration 3.6.3 HF cartridges UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 69: Uniflux 30 Hf Configuration
3.6.1 UniFlux 30 HF configuration 3.6.1 UniFlux 30 HF configuration Illustration: Hollow fiber size 75 An optional spool piece kit is needed to mount size 75 hollow fibers on the system. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 70 3 System description 3.6 Hollow fiber (HF) configuration 3.6.1 UniFlux 30 HF configuration Illustration: Hollow fiber size 55 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 71 Description of HF components Part Function Retentate line, rearranged to upper position for HF configuration Hollow fiber cartridge Permeate outlet hose Feed line extension pipe HF cartridge drain hose UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 72 3 System description 3.6 Hollow fiber (HF) configuration 3.6.1 UniFlux 30 HF configuration Part Function HF cartridge drain valve (XV-065) UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 73: Uniflux 120 Hf Configuration
3.6.2 UniFlux 120 HF configuration 3.6.2 UniFlux 120 HF configuration Illustration: Hollow fiber size 75 An optional spool piece kit is needed to mount size 75 hollow fibers on the system. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 74 3 System description 3.6 Hollow fiber (HF) configuration 3.6.2 UniFlux 120 HF configuration Illustration: Hollow fiber size 55 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 75 Description of HF components Part Function Retentate line mounted in upper position Retentate collector manifold Hollow fiber cartridges (2) Permeate hoses (2) Permeate collector manifold Feed extension pipes (2) UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 76 3 System description 3.6 Hollow fiber (HF) configuration 3.6.2 UniFlux 120 HF configuration Part Function Permeate drain hoses (2) Permeate drain collector manifold (2) Permeate drain valve (XV-065) UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 77: Hf Cartridges
Available types of HF cartridges UniFlux 30 and UniFlux 120 systems are designed to accept Hollow fiber cartridges of different sizes. These cartridges are described in the Hollow fiber cartridges for membrane separations Operating Handbook Cytiva article no 18116530 and in the Hollow fiber cartridges and systems for membrane separations Selection Handbook Cytiva article no.
Page 78 When mounted vertically, which is the case for all UniFlux systems, the bottom connection serves as drain for the permeate. This allows for more complete recovery of the permeate. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 79: Meters And Sensors
This section describes the meters and sensors that are available as basic configuration and optional components for UniFlux system. In this section Section See page 3.7.1 Pressure 3.7.2 Flow 3.7.3 pH, conductivity and UV 3.7.4 Temperature 3.7.5 More information UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 80: Pressure
The pump outlet pressure sensors also provide feed pump and system over pressure protection. More information on the pressure sensors is provided in the system documentation package. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 81: Flow
Illustration of flow sensors FT-142 FT-141 Description FT-141 measures the feed flow and FT-142 measures the permeate flow. The retentate flow is calculated by UNICORN by subtracting the permeate flow from the feed flow. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 82: Ph, Conductivity And Uv
The optional conductivity sensor CE-101 measures permeate conductivity. Calibra- tion at regular intervals is recommended. There are two conductivity ranges for UniFlux 30 and 120 which can be changed in UNICORN. Calibration must be done after the change. The optional UV sensor AE-131 measures the UV absorption in the permeate line.
Page 83: Temperature
3 System description 3.7 Meters and sensors 3.7.4 Temperature 3.7.4 Temperature Illustration of temperature sensor TE-161 Description Temperature is measured in the retentate line with the sensor TE-161. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 84: More Information
Sensor signals are described in detail in Section 8.2 Signals, on page 184. Location and tagging of sensors Location and tagging of each sensor is described in Section 3.8 Flowcharts, on page UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 85: Flowcharts
3 System description 3.8 Flowcharts Flowcharts About this section This section contains flowcharts and interface points descriptions for the UniFlux 30 and UniFlux 120 systems. In this section Section See page 3.8.1 External connections 3.8.2 Flow diagrams UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 86: External Connections
3 System description 3.8 Flowcharts 3.8.1 External connections 3.8.1 External connections Introduction This section describes the external connections on UniFlux 30 and 120 and the optional tank. Connection diagram HTPN4 TME3 TME4 Legend Symbol Color Description Blue Outlets Orange Inlets...
Page 87 Feed drain Outlet Interface points Diagram color: Green Interface points provide process connections between the UniFlux system and the optional UniFlux tank. Hoses for these connections are normally delivered by Cytiva as option to the tank delivery. Function Direction ME5 - Feed from optional transfer pump to tank System ->...
Page 88: Flow Diagrams
Flow diagrams Introduction This section contains complete flowcharts indicating standard and optional process components of UniFlux 30 and UniFlux 120 systems. Standard components of are indi- cated in black. Optional components of are indicated in red. UniFlux 30: Flow diagram...
Page 89 UniFlux 120: Flow diagram CE/TE -101 AE-121 AE-131 FT-142 PT-114 PT-112 FT-141 PT-113 TE-161 Component description Tag / System Function Note More information AE-121 Permeate pH sensor Option Section 3.7.3 pH, conduc- UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 90 55 Transfer pump inlet (from Part of transfer Transfer pump P-202 on buffer) pump option page 54 Transfer pump outlet (to tank) Retentate outlet Section 3.3.5 Retentate line, on page 48 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 91 Filter integrity test pressure regulator PT-111 Feed pump outlet pressure Section 3.7.1 Pressure, on meter page 80 PT-112 Feed pump outlet pressure meter PT-113 Retentate pressure meter PT-114 Permeate pressure meter UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 92 Quadruple inlet 1, 2, 3 and 4 Part of quadruple Section 3.4.3 Multiple inlets, 083, 084 valves inlet option on page 55 XY-342 Filter integrity test valve Section 3.3.7 Air integrity test device, on page 51 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 93: Unicorn Control System
A part 11-system assessment checklist is available on request through the local Cytiva representative. Prerequisite knowledge A working knowledge of UNICORN is required to operate UniFlux 30 and UniFlux 120 systems. Information on how to use UNICORN can be obtained from the UNICORN User Manuals and available tutorials.
Page 94 Step Action Program a UniFlux 30 system method run using the UNICORN software. It is possible to use an existing method or modify an existing method to meet your run objectives. Start the run using the method you created.
Page 95 System alarm: Indicator Check UNICORN for detailed information. Warnings Warnings are generated to warn operating personnel that process parameters have exceeded preset high and/or low limits, and that the process method continues. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 96 →Manuals from the UNICORN top menu. The UNICORN user reference manual for BioProcess™ is especially suited for usage with BioProcess systems. Information about how to create a method for UniFlux 30 in the UNICORN Method Editor is presented in the end of this Operating Instructions.
Page 97: Installation
4 Installation Installation About this chapter This chapter provides required information to enable users and service personnel to unpack, install, move and transport UniFlux 30 and UniFlux 120 systems In this chapter Section See page Site requirements Transport Unpacking UniFlux Setup...
Page 98: Site Requirements
Section 4.4.5 Connect compressed air supply, on page 111 regarding connec- tions to the instrument. Media supply Supply must be arranged so that piping dimensions, piping lengths, valves and height differences do not obstruct processing. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 99 Computer An industrial built-in computer can be ordered as an option for UniFlux 30 and UniFlux 120, otherwise a stand-alone computer shall be used. When installing a stand-alone computer ensure that it is installed with appropriate precautions for the intended envi- ronment that might expose the computer to liquids and moisture.
Page 100: Transport
The console arm is only designed to support the weight of the operator console. UniFlux 30 and UniFlux 120 systems can be rolled by hand on hard and level surfaces with wheel brakes released. If the floor quality does no allow rolling the system on its own wheels, it can be moved with a pallet jack or forklift.
Page 101: Unpacking
• 13 mm wrench (or ratchet with 13 mm socket). • Electrical screwdriver with no. 2 bit. • Knife. • Lever (enclosed in crate). Note: Refer to the unpacking instructions for UniFlux system (art. no 29065657) for detailed unpacking instructions. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 102 • Check that all equipment is enclosed in the crate according to the packing list. • Check that the equipment for any apparent damage and document carefully if found. If any equipment is missing or damage is found, contact your Cytiva representative immediately. General unpacking instructions...
Page 103: Uniflux Setup
4.4.2 Operator console 4.4.3 Stand-alone computer 4.4.4 Setup of control system and network 4.4.5 Connect compressed air supply 4.4.6 Filter setup, feed pump and CFF filter protection 4.4.7 Process component connections UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 104: Lock The Wheels
4 Installation 4.4 UniFlux Setup 4.4.1 Lock the wheels 4.4.1 Lock the wheels After positioning the system at its designated location, lock the wheels as illustrated below. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 105: Operator Console
Except for the console, the UniFlux system is delivered fully assembled at production site arrival. No further specific assembly procedures are required. Follow this sequence when fitting the operator console to the UniFlux 30 and 120 systems: UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 106 Carefully slide the console arm over the system handle (B) until it is firmly attached. Keep the foam protection (A) in place when attaching the console arm. Remove the foam protection. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 107 The protective screw cap (D) should always be in place when the USB connector is not in use. Fix the cables neatly together with the existing cables underneath the cabinet using cable ties, as illustrated below. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 108 4.4 UniFlux Setup 4.4.2 Operator console Step Action Press the Rotation Stop on to the console arm as shown in the picture and push it into place around the rotation center. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 109: Stand-Alone Computer
UNICORN ETHERNET connector illustrated below. Note: When installing a stand-alone computer ensure that it is installed with appropriate precautions for the intended environment that might expose the computer to liquids and moisture. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 110: Setup Of Control System And Network
To use the Ethernet connection, the CU-960 must be set up like a network device with IP address, default gateway and subnet mask address. Refer to the control unit manual for more information. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 111: Connect Compressed Air Supply
Section 4.1 Site requirements, on page Connect compressed air supply to UniFlux 30 and UniFlux 30 Step Action Connect compressed air to the AIR INLET on the electrical cabinet. Part Function Air exhaust Air inlet UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 112 Make sure that the supply pressure to the cabinet is between 5.5 and 7 bar g. The manometer is set to 6 bar g at the factory and shall not be changed. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 113: Filter Setup, Feed Pump And Cff Filter Protection
If such a filter screen becomes clogged, the flow to the feed pump will be restricted and the pump may be damaged because of cavitation. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 114: Process Component Connections
UniFlux system UniFlux tank Retentate recycle UniFlux system UniFlux tank Transfer of media UniFlux system UniFlux tank transfer pump Transfer of media (not Plant upstream UniFlux system visible) process transfer pump UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 115 • Route the tubing to minimize the risk of tripping when operating the instrument and connected device. • If the hoses should be violently pulled caused by an accidental tripping, they should be checked for damage, leakage and mechanical rigidity before continuing opera- tion. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 116 4 Installation 4.4 UniFlux Setup 4.4.7 Process component connections • The chemical properties of used tubing shall meet or exceed the properties speci- fied in Section 8.4 Chemical resistance, on page 188. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 117: Power Supply
Cytiva does not offer a UPS (Uninterrupted Power Supply) as an accessory. However, the system is prepared for UPS operation. For UPS operation, a UPS should be connected to the main power supply of UniFlux 30 and UniFlux 120 systems.
Page 118 The power cord wires are color coded as shown in the table below. They must be connected to the corresponding terminals in the fixed power supply or to a connector compatible with IEC 60309-2. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 119 The mains power supply to the UniFlux system and the pumps are equipped with circuit breakers inside the electrical cabinet. Refer to the System Documentation for the specific types used in your system. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 120 More information Wiring diagrams for the system, voltage, power, fuse requirements and the tripping current for the fixed power supply ground fault protector can be found in the system documentation package. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 121: Operation
5 Operation Operation About this chapter This chapter provides the information required to operate UniFlux 30 and UniFlux 120 systems in a safe way. In this chapter Section See page Prepare the system Calibrations Perform a run Procedures after usage...
Page 122 The wetted parts of the product may be damaged by chemicals not listed in the Chemical Resistance information. Contact your Cytiva representative before using chemicals that are not listed. Section 8.4 Chemical resistance, on page 188 for more information. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 123: Prepare The System
5 Operation 5.1 Prepare the system Prepare the system About this section This section describes the steps that should be performed to prepare UniFlux 30 and UniFlux 120 systems for a run. In this section Section See page 5.1.1 Sample handling 5.1.2...
Page 124: Sample Handling
NFF can be used when recovering the processed product from the system. Recommendations The table below describes the situations where the Cytiva range of ULTA™ NFF capsules can be used. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 125 Filtered material to UniFlux feed inlet Feed from tank to system Retentate from system to tank Permeate out Recovered material from UniFlux after completed CFF run ULTA NFF filtered recovered material UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 126 5 Operation 5.1 Prepare the system 5.1.1 Sample handling Part Function Container for recovered material, preferably a ReadyCircuit™ bag (part of the ReadyToProcess™ portfolio) or equal UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 127: Start The System And Software
For some UNICORN versions it is also possible to select the Use Windows Authentication checkbox and enter a network ID in the user name field. Click OK. In the System Control module, select System →Connect. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 128 When UNICORN is connected to the system, the Run button in the status bar is enabled. The button initially has a green color indicating the system is ready to run. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 129: Install Hollow Fiber Cartridges
Section 6.7 Change filter type, on page 167 if required. Prepare the HF cartridge(s) according to the manufacturer's instructions. Install the cartridge into the system, refer to illustrations in Section 3.6 Hollow fiber (HF) configuration, on page UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 130: Install Filter Cassettes
For all filter cassettes, empirical testing is needed to decide what hydraulic pressure to use. Contact your Cytiva representative for advice. Preparations Step Action Prepare and check the cassettes according to the manufacturer's instruc- tions. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 131 PAUSE is on and steady. Make sure that the hydraulic unit is switched Make sure that the key on the hydraulic unit front side is switched to ON and the white POWER light is UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 132 Remove any hydraulic piston extensions attached to the hydraulic piston by unscrewing the extensions. Slide the cassette holder pressurization back plate backwards by hand until it meets the hydraulic cylinder piston. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 133 Installation Note: This assembly instruction applies to Cytiva cassettes only. For information on installing other cassettes, please contact your local Cytiva representa- tive. Step Action To install one cassette, clean and wet a silicone gasket by rinsing it with DI water or water-for-injection (WFI).
Page 134 Pressurization CAUTION The operator must always make sure that no one is present and exposed before energizing the hydraulic pump. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 135 5 Operation 5.1 Prepare the system 5.1.4 Install filter cassettes Using the hydraulic pump, pressurize the hydraulic cylinder according to the previously performed calculation. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 136: Priming And Testing
Perform a leakage test. Perform an air integrity test. Priming the lobe rotor pump Priming is needed for UniFlux 30 and UniFlux 120 systems. The CIP valve (XV-062) is used in order to prime the lobe rotor pump. NOTICE Do not start the lobe rotor pump before it is primed with the intended media.
Page 137 Section 4.4.5 Connect compressed air supply, on page 111. Isolate the upstream process by closing the following valves: • Feed valve XV-001. • Retentate pressure control valve PCV-341. • Retentate valve XV-051. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 138 When the PT-113 pressure indicator reads zero, the system is depressur- ized and the test procedure completed. Unexpected flowrate If the flow deviates from the characteristic flow during an air integrity test, the following conclusions can be drawn: UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 139 The fibers or membranes are broken. The filter will allow larger particles than the nominal cut-off size to pass to the permeate and must hence not be used for processing any more. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 140: Calibrations
This section describes how to establish and enter hold-up volume, prepare mass meas- uring, control meter range and calibration. In this section Section See page 5.2.1 Preparing mass measuring 5.2.2 Establishing and entering holdup volume 5.2.3 Meter range and calibration UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 141: Preparing Mass Measuring
The measuring scale must then be set with regard to zero point and slurry density if the density differs significantly from 1.0. Calibration: Mass measuring, on page 165 for the calibration procedure. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 142: Establishing And Entering Holdup Volume
In UNICORN System Control module, select System →Settings. b. In the UniFlux Instructions dialog, select Specials and System_Volume. c. In the Skid field, enter the correct holdup volume. Click OK. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 143: Meter Range And Calibration
165. Conductivity calibration If necessary, calibrate the conductivity meter. See Conductivity test, on page 166. pH-calibration Calibrate the pH sensor. See Section 6.5 pH calibration, on page 163. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 144: Perform A Run
Final checks before start NOTICE Make sure that the actions listed below are completed before UniFlux is started. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 145 UNICORN manual package. End the run, normal completion If no unexpected events occur during the run, UNICORN enters END state at method completion without need for user interaction. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 146 In the dialog, you can choose to save the (partial) results from the run so far. If the run is part of a scouting run, you are also given the choice of ending this. (If you do not, the next run will start automatically.) UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 147: Procedures After Usage
Preparing for storage Prepare the system for storage as described in Section 6.3 Storage, on page 158 required. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 148: Maintenance
6 Maintenance Maintenance About this chapter This chapter provides required information to enable users and service personnel to clean, maintain, calibrate and store the UniFlux 30 and UniFlux 120 systems. In this chapter Section See page User maintenance schedule Cleaning...
Page 149 • Always perform a leakage test before operation or CIP. WARNING Decontaminate before service. Before performing any service work on the system, make sure that the system has been properly decontaminated. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 150: User Maintenance Schedule
Connections and Check for leakage. Replace seals if needed. Perform a leakage seals test at maximum operating pressure. Annually or as needed This section covers maintenance actions required annually or as needed. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 151 Replace the UV lamp when the intensity is low or when a lamp failure is indicated in UNICORN. pH sensor Replace the pH electrode if difficulties are experienced during calibration. Conductivity Clean and calibrate the conductivity monitor. monitor UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 152: Cleaning
6.2 Cleaning Cleaning About this section This section describes procedures and recommendations for cleaning the UniFlux 30 and UniFlux 120 systems. Procedures for cleaning both the exterior, and CIP (cleaning- in-place) protocols for cleaning the flow path are described. In this section...
Page 153 CIP with strong acids or alkali. NOTICE The filter may not be compatible with the CIP solutions. If so, remove the filter and store or dispose of it in accordance with the recommendations of the manufacturer. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 154: Important Considerations For Cleaning
The UV cell and conductivity cells are cleaned by flushing the cells with 1 M sodium hydroxide or 20% ethanol. Clean the filters • Cytiva Hollow fiber cartridges cleaning procedures are provided in the Hollow fiber cartridges for membrane separations Operating Handbook Cytiva article no 18116530.
Page 155 It is not recommended to spray or splash liquids towards external surfaces. Do not spray or splash liquids towards filters or the fan on the electrical cabinet. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 156: Cleaning-In-Place (Cip)
• Extending the total CIP time period. • Changing to an alternative CIPagent. • Filling the UniFlux system and attached components with cleaning agent to redis- solve contaminants for an extended time period before applying CIP. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 157 After completed CIP, rinse the UniFlux system interior and all cleaned components thoroughly with water of desired quality, for example Water For Injection (WFI), to remove all traces of the cleaning agent. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 158: Storage
6 Maintenance 6.3 Storage Storage Introduction This section provides instructions for short-term and long-term storage of UniFlux 30 and UniFlux 120 systems. Precautions WARNING Hazardous substances and biological agents. When using hazardous chemical and biological agents, take all suitable protec- tive measures, such as wearing protective clothing, glasses and gloves resistant to the substances used.
Page 159 Make sure that the filter cassettes are at least partially wetted during the storage. Seal off the UniFlux system to prevent contamination caused by the surrounding environment. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 160 The reference below provides storage procedures for Hollow fiber filters. • Cassettes storage instructions are provided by the filter cassette manufacturer. • Cytiva Hollow fiber cartridges storage procedures are provided in the Hollow fiber cartridges for membrane separations Operating Handbook, Cytiva article no 18116530.
Page 161: Disassembly And Assembly
• Always perform a leakage test after assembly or maintenance. • Always perform a leakage test before operation or CIP. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 162 Other than described in this chapter, the UniFlux is not designed to be disassembled or assembled by the user. If the need for further disassembly should arise, always contact your Cytiva represen- tative for advice before attempting any actions not described by this document.
Page 163: Ph Calibration
Select System →Calibrate in UNICORN System Control module. Enter the pH value for the first buffer in the Reference value 1 field, wait for the value to stabilize, and click Read value 1. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 164 Close, refresh/change the pH electrode, and repeat the calibration proce- dure. Remove the cup from the holder. Relocate the pH probe in the flow cell as shown in the right image above. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 165: Repair And Calibration
Introduction This section describes component checks, repair and calibration of other components (beside the pH probe) that the user can perform without Cytiva support. Procedures can also be found in the respective product manuals found in the product documentation package.
Page 166 NOTICE Waste batteries and accumulators must not be disposed of as unsorted municipal waste and must be collected separately. Follow applicable local regulations for recycling of batteries and accumu- lators. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 167: Change Filter Type
While system filter type reconfiguration can be performed by a properly skilled user technician, Cytiva recommends that a Cytiva service engineer is assigned for this work. Contact your Cytiva representative for more information on changing filter configurations if required. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 168 Move the retentate process line from the lower to the upper position. Remove all cassette holder fittings. Install the HF drain valve, XV-065 and connect the control cables to the system. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 169 Remove the HF-drain valve, XV-065. Remove the HF-specific parts of the piping system for retentate and feed. Install the spillage plates under the cassette holder and the hydraulic unit. Install the hydraulic unit. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 170 Install the cassette holder and connect it to the feed, permeate and reten- tate process lines. Install a dummy or decommisioned filter cassette and test the system for operation and leakages. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 171: Troubleshooting
UniFlux system. If the suggested actions in this guide do not solve the problem, or if the problem is not covered by this guide, contact your Cytiva representative for advice. Precautions...
Page 172 • No inlet or outlet valve open. Check method and valves. • Incorrect method. Check by entering Pump →ManFlow > 1%. • If none of the above, contact Cytiva service personnel. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 173 Bad UV fiber connec- Check the connections of the drift or instability. tions. UV cell optical fiber. Replace if necessary. Dirty UV cell. Clean the UV cell, see Section 6.2 Cleaning, on page 152. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 174 Check all connections for leaks. Blockage or partial Flush through to clear blockage of flow path. blockage. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 175 The oil level should be approximately 60% of the reservoir's volume (3 L), then the hydraulic cylinder and the tubes and hoses should be filled with oil. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 176 Incorrect or unstable Bad pump or valve Check the pump and the reading. action. valves. Temperature compen- Check the temperature sation not properly set. compensation, see manufac- turer's user documentation. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 177 Calibrate the pH-sensor as be incorrect. calibrated. described in Section 6.5 pH calibration, on page 163. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 178: Reference Information
About this chapter This chapter provides reference information that may become useful when installing, operating, maintaining and troubleshooting UniFlux 30 and UniFlux 120 systems. It also contains ordering information. The UniFlux system is a modular built instrument that can be configured in many different ways.
Page 179: Specifications
• Dimensions and weights are valid for standard systems. • Dimensions may vary for individual units by +/- 1 cm. • Weights may vary for individual units by +/- 10 kg. UniFlux 30 UniFlux 120 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 180 120 L/min Feed transfer- and permeate 1.9 L/min control pump option, flow at 19 L/min 2 bar outlet backpressure Feed tank External and optional, refer to UniFlux Feed Tank Operating Instructions. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 181 General Specification included in the system documentation kit Ingress protection IP 55 Compressed air Property Value Pressure 6 to 10 bar (87 to 145 psi) Consumption 50 Nl/min Type Particle-free and non- condensing UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 182 Measured at the feed flow rate 40 L/min and feed pressure 2 bar Property UniFlux 120 Feed pump <90 dB Typical value under normal running 77 dB conditions Measured at the feed flow rate 80 L/min and feed pressure 2 bar UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 183 System System holdup volume UniFlux 30 1 liter UniFlux 120 3 liters UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 184: Signals
Pressure PT-111, bar g 0 to 6 0.1 to 6 PT-112 Flow FT-141 L/min 0 to 175 12 to 120 Retentate Pressure PT-113 bar g 0 to 6 0.1 to 6 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 185 100 to 2000 Hydraulics Pressure PT-291 0 to 170 0 to 170 Feed tank High On / Off level, sensors On / Off provided by customer Meter 0 to 100 0 to 100 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 186: Battery Limits
Pneumatic Battery limit Label Size and connector type AIR INLET Air inlet Quickfit, female, hose 10 x 1 mm Air outlet AIR OUTLET Quickfit, female, hose 10 x 1 mm UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 187 LT-167 Analog level sensor 5-pole connector input ETHERNET 8 pas. connector LAN/WAN Ethernet port on (modular RJ45) system USB connection USB on operator console Note: Present with the optional operator console. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 188: Chemical Resistance
Introduction The table below gives allowed exposure concentrations and times for various chemi- cals that may be used in Cytiva BioProcess™ instruments in general. For information regarding hollow fiber chemical resistance, please refer to Hollow fiber cartridges for membrane separations Operating Handbook, Cytiva article no 18116530.
Page 189 Urea 5000 h Purification, Cleaning solu- 1% to 6% STERIS™ 3 h at temp ≤ 60°C 3000 h tions CIP 100™, 0.5% Henkel P3™-11, 0.2% Micro, 0.2% Terg-a-zyme™, 0.1% Tween™ 80 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 190: Recycling Information
Disposal, general instructions When taking UniFlux 30 and UniFlux 120 systems, out of service, the different mate- rials must be separated and recycled according to national and local environmental regulations.
Page 191: Regulatory Information
European Union and European Economic Area 8.6.3 Eurasian Economic Union Евразийский экономический союз 8.6.4 Regulations for North America 8.6.5 Regulatory statements 8.6.6 Declaration of Hazardous Substances (DoHS) 8.6.7 Other regulations and standards UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 192: Contact Information
The table below summarizes the required manufacturing information. Requirement Information Name and address of manufacturer Cytiva Sweden AB Björkgatan 30 SE 751 84 Uppsala Sweden Telephone number of manufacturer + 46 771 400 600 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 193: European Union And European Economic Area
• used according to the Operating Instructions or user manuals, and • used in the same state as it was delivered, except for alterations described in the Operating Instructions or user manuals. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 194: Eurasian Economic Union
В следующей таблице приводится сводная информация о производителе и импортере, согласно требованиям Технических регламентов Таможенного союза и (или) Евразийского экономического союза. Требование Информация Информацию об изготовлении Наименование, адрес и номер См. телефона производителя UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 195 Member States of the Customs Union of the Eurasian Economic Union Данный знак о Евразийском соответствии указывает, что изделие одобрено для использования на рынках государств-членов Таможенного союза Евразийского экономического союза UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 196: Regulations For North America
Note: The user is cautioned that any changes or modifications not expressly approved by Cytiva could void the user’s authority to operate the equip- ment. This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the FCC Rules.
Page 197: Regulatory Statements
When used in a residential environment, there is a concern of radio interference. 주의 A급 기기 (업무용 방송통신 기자재) 이 기기는 업무용환경에서 사용할 목적으로 적합성평가를 받 은 기기 로서 가정용 환경에서 사용하는 경우 전파간섭의 우려가 있습 니다. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 198: Declaration Of Hazardous Substances (Dohs)
Periodic replacement of those consumables or parts to maintain the declared EFUP shall be done in accordance with the Product Maintenance Procedures. This product must not be disposed of as unsorted municipal waste, and must be collected sepa- rately and handled properly after decommissioning. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 199 Indicates that this hazardous substance contained in at least one of the homogeneous materials used for this part is above the limit requirement in GB/T 26572 • Data listed in the table represents best information available at the time of publication. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 200: Other Regulations And Standards
Biological Reactivity Tests, "In Vivo". Additional design considerations For additional design considerations, such as ASME-BPE, GMP and cGMPs information refer to the product-specific specification information provided in the documentation package delivered with each equipment. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 201: Further Information
Spare parts and accessories Additional information regarding spare parts and acccessories can be found in the system documentation. Your local Cytiva representative will also be able to suggest recommended spare parts and accessories. Remaining aspects Regarding any of the below topics, please contact your local Cytiva representative for advice.
Page 202: Health And Safety Declaration Form
Service Ticket #: To make the mutual protection and safety of Cytiva service personnel and our customers, all equipment and work areas must be clean and free of any hazardous contaminants before a Service Engineer starts a repair. To avoid delays in the servicing of your equipment, complete this checklist and present it to the Service Engineer upon arrival.
Page 203 To make sure the mutual protection and safety of Cytiva personnel, our customers, transportation personnel and our environment, all equipment must be clean and free of any hazardous contaminants before shipping to Cytiva. To avoid delays in the processing of your equipment, complete this checklist and include it with your return.
Page 204: A Appendix A: Create A Method With Unicorn 5
For information about methods in general, and for detailed information about the Method Editor, refer to the UNICORN manuals. In this chapter Section See page The Method Editor An example of using the Method Editor UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 205: The Method Editor
A Create a method with UNICORN 5 A.1 The Method Editor The Method Editor In this section Section See page Introduction to the Method Editor Method Editor layout Other Method Editor features UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 206: Introduction To The Method Editor
In the dialog shown, give the block a name and select the type of Base (Time or Volume) for the block. The choice of base depends on the function of the block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 207 Customize Panes dialog. These diagrams can help when programming advanced methods, but are not required for building or editing a method. They are therefore not included in the following discus- sion. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 208: Method Editor Layout
Replace and Delete affect the instruction or block currently selected in the Text pane. Insert adds a new instruction before, or after (if it has the same breakpoint as an old instruction), the current selection. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 209 Note: There is no undo-function in UNICORN for BioProcess and you are therefore recommended to regularly save the method you are working with, pref- erably using different version numbers. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 210: Other Method Editor Features
Insert watch instruction Watch_FT142VTot (under Watch) and set values for Test (e.g., Greater_Than), Value (volume in liters) and Action (e.g., END_BLOCK). The allowed noise, or error, for a totalizer can be defined using the appropriate FT14VTot_WatchPar instruction (under Specials). UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 211: An Example Of Using The Method Editor
An example of using the Method Editor In this section Section See page Introduction Create the method Block 1: Initial conditions Block 2: Product transfer Block 3: Fed-batch concentration Saving the method UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 212: Introduction
The method used in this example is for training purpose only. It is not intended as an example of a production method. Tip: Modify an instruction by selecting it in the Text pane and make the changes in the Instruction box. Tip: UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 213 Editor is used. Installed by default at initial delivery if the optional built-in computer has been ordered. If the UniFlux system has a built-in computer, this occurs automatically at system power- UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 214: Create The Method
A.2 An example of using the Method Editor Create the method Create the method This step creates the method and sets some initial conditions to it. Step Action Select File →New in the Method Editor menu. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 215 Select Method Editor (pre-selected). c. Click OK. In the New Method dialog shown: The new method will be displayed in the Text pane as the Main block with a single Base Time instruction. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 216 • In the Instructions group box, select Other and Flow_Warning. • In the Parameters field, set Mode to Disabled. • Click the Insert button. The new instruction will now show up in the Text pane below Base Time. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 217: Block 1: Initial Conditions
• In the Name field, type an appropriate name, for instance INITAL_CONDITIONS (space is not allowed). • In the Base group box, select Time. • In the Call group box, select Main. • Click OK to insert the new block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 218 • In the Parameters field, set Area to the correct value in m2, depending on your filter setup. • Click the Insert button. The new instruction will now be displayed in the Text pane. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 219 Low Warn to appropriate values according to the specifications for the filter in use or requirements of the intended application (if lower than filter specifications). • In the Parameters field, set Mode to Enabled. • Click the Insert button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 220: Block 2: Product Transfer
• In the Base group box, select Time. • In the Call group box, select Block PRODUCT_TRANSFER. • Click OK to insert the new block. The new block will be placed inside the Block PRODUCT_TRANSFER block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 221 • In the Instructions group box, select Permeate. Select Permeate, set to Closed, click Insert. Select Permeate_Drain, set to Closed, click Insert. Progress review The Method Editor should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 222 In the Text pane, select the End_Block instruction of the Block DELAY_3_SEC block. In the Instruction box: • Set Breakpoint to 0.05 min (equals 3 seconds). • Click the Change button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 223 • In the Instructions group box, select Recirc. • Select TransferFeedPump, set Flow to 90%, click Insert. Instruction: Tank level watch Step Action In the Text pane, select the Base Time instruction of the Block PRODUCT_TRANSFER_VOLUME block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 224 • Set Breakpoint to a value high enough for the tank to reach the volume set for WatchTankLevel (in the previous step). • Click the Change button. Progress review The Method Editor window should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 225 A Create a method with UNICORN 5 A.2 An example of using the Method Editor Block 2: Product transfer UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 226: Block 3: Fed-Batch Concentration
• In the Call group box, select From →Block FED_BATCH_CONCENTRATION and At →0.00 min. • Click OK to insert the new block. The new block will be placed inside the Block FED_BATCH_CONCENTRATION block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 227 Here, the 3 second delay block created earlier will be reused to allow all valves to react. Step Action In the Text pane, select the last instruction before the End_Block instruc- tion of the Block FLOWPATH_CONCENTRATION block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 228 In the Text pane, select the Base Time instruction of the Block PARAMETERS_CONCENTRATION block. In the Instruction box: • Set Breakpoint to 0.00 min. • In the Instructions group box, select Recirc and TankLevel. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 229 In the Text pane, select the Base Time instruction of the Block PARAMETERS_CONCENTRATION block. In the Instruction box: • Set Breakpoint to 0.00 min. • In the Instructions group box, select Permeate and FIT142Tot_Reset. Click Insert. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 230 Click Insert. Progress review The Method Editor should now look something like this. Nested block: Stable differential pressure (Delta P) This block ensures that a stable Delta P value is reached. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 231 In the Text pane, select the Base Time instruction of the Block STABLE_DELTA_P block. In the Instruction box: • Set Breakpoint to 0.00 min. • In the Instructions group box, select Specials and DeltaP_WatchPar. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 232 In the Text pane, select the End_Block instruction of the Block STABLE_DELTA_P block. In the Instruction box, set Breakpoint to 10.00 min. Click Change. Nested block: Trans membrane pressure (TMP) setup This block sets the TMP value. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 233 In the Text pane, select the Base Time instruction of the Block TMP_START_STOP block. In the Instruction box: • Set Breakpoint to 0.00 min. • In the Instructions group box, select Watch and Watch_FIT142VTot. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 234 In the Instruction box, set Breakpoint to a suitable time, for instance 60.00 min. Click Change. Progress review If all the steps described above have been performed, the Method Editor window should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 235 A.2 An example of using the Method Editor Block 3: Fed-batch concentration If any step appears to be missing, misplaced or carries incorrect values, use the various editing methods available in the Method Editor to correct the errors. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 236: Saving The Method
A Create a method with UNICORN 5 A.2 An example of using the Method Editor Saving the method Saving the method To save the method, click the discette icon or select File →Save in the menu. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 237: Appendix B: Evaluation For Unicorn 5
User Reference Manual - UNICORN 5.1 - Evaluation for Cross Flow Filtration. Opening the evaluation module In UNICORN Evaluation click the Membrane System Evaluation icon. The Operation dialog is displayed. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 238 Check the box for the first chromatogram, e.g. “crossflow 1” and click the New data group button. Check the box for the second chromatogram, e.g. “crossflow 2” and click the New data group button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 239 The image shows an example of plotted Normalized Water Flux. Capacity plots Use this function to create a graphical analysis of the filter capacity during a filtration. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 240: Appendix C: Create A Method With Unicorn 6 And Above
For information about methods in general, and for detailed information about the Method Editor, refer to the UNICORN manuals. In this chapter Section See page The Method Editor An example of using the Method Editor UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 241: The Method Editor
C Create a method with UNICORN 6 and above C.1 The Method Editor The Method Editor In this section Section See page Introduction to the Method Editor Method Editor layout Other Method Editor features UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 242: Introduction To The Method Editor
Select the User Defined phase in the Phase Library pane and drag-and- drop the phase to the requested position in the Method Outline pane. Result: The phase is included in the method at the requested position. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 243 Follow this general procedure to create a block in the Method Editor. Step Action In the Instruction Box: In the Instructions: list, select Other and Block. In the Parameters for field, type an appropriate name. Click the Insert button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 244: Method Editor Layout
Instructions tab are displayed and available for editing in the Instruction Box below the Text Instructions tab. • Breakpoint — Contains a field for setting the breakpoint for the instruction or block, in the unit currently selected as Base (Time or Volume). UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 245 Changing valve positions, or changing the speed of the pump does not take place momentarily, but requires a small amount of time. A small time lag, such as 0.10 min, should therefore be introduced after time-based instructions affecting mechanical components. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 246: Other Method Editor Features
(FT142Vtot), Test (e.g., Greater_Than), Value (volume in liters) and Action (e.g., End block). The allowed noise, or error, for a totalizer can be defined using the appropriate FT14VTot_WatchPar instruction (under Specials). UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 247: An Example Of Using The Method Editor
An example of using the Method Editor In this section Section See page Introduction Create the method Phase 1: Method Settings Phase 2: Product Transfer Phase 3: Fed Batch Concentration Saving the method UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 248: Introduction
The method used in this example is for training purpose only. It is not intended as an example of a production method. Tip: Modify an instruction by selecting it in the Text Instructions tab and make the changes in the Instruction Box. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 249 Editor is used. Installed by default at initial delivery if the optional built-in computer has been ordered. If the UniFlux system has a built-in computer, this occurs automatically at system power- UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 250: Create The Method
C.2 An example of using the Method Editor Create the method Create the method The following step creates the method and sets some initial conditions to it. Step Action Select File →New Method in the Method Editor menu. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 251 Under the Main node, select the Base: instruction. d. In the Instruction Box, select Other and set the instruction Base parameter Column Type to Any, and the Base parameter to Time. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 252: Phase 1: Method Settings
In the Text Instructions tab, expand the Phase →Method Settings phase by pressing the + to the left of the phase. Select the Base →SameAsMain instruction of the Phase →Method Settings phase. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 253 • In the Instructions: list, select Other and Flow_Warning. • In the Parameters for field, set Mode to Disabled. • Click the Insert button. The new instruction will now show up in the Text Instructions tab below Base →SameAsMain. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 254 The new instruction will now be displayed in the Text Instructions tab. Instructions: Filter pressure alarm Step Action In the Text Instructions tab, select the Base →SameAsMain instruction of the Phase →Method Settings phase. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 255 Low Warn to appropriate values according to the specifications for the filter in use or requirements of the intended application (if lower than filter specifications). • In the Parameters for field, set Mode to Enabled. • Click the Insert button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 256: Phase 2: Product Transfer
To include instructions for the Phase →Product Transfer phase, select the Text Instructions tab and text edit the method. Note: The Phase Properties tab will show a list of variables used in the phase. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 257 Select Retentate_Control_Valve_PCV341, set to 100%, click Insert. Select Retentate, set to Retentate, click Insert. Instruction: Permeate outlets Step Action In the Text Instructions tab, select the Base: SameAsMain instruction of the Block →PROD_TRANSFER_FLOWPATH block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 258 • In the Instructions: list, select Permeate. Select Permeate, set to Closed, click Insert. Select Permeate_Drain, set to Closed, click Insert. Progress review The Method Editor should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 259 • Set Breakpoint to 0.00 min. • In the Instructions: list, select Other and Block. • In the Parameters for field, type an appropriate name, for instance PROD_TRANSFER_VOLUME. • Click the Insert button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 260 In the Instruction Box: • Set Breakpoint to a value high enough for the tank to reach the volume set for Watch (in the previous step). • Click the Change button. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 261 C Create a method with UNICORN 6 and above C.2 An example of using the Method Editor Phase 2: Product Transfer Progress review The Method Editor window should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 262: Phase 3: Fed Batch Concentration
The Fed Batch Concentration phase is marked with the letter T, meaning that it is text edited. This phase contains only Base and End_Block instruc- tions, so any functional instructions must be added manually. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 263 In the Text Instructions tab, select the Base →SameAsMain instruction of the Block →FLOWPATH_CONCENTRATION block. In the Instruction Box: • Set Breakpoint to 0.00 min. • In the Instructions: list, select Recirc. Set Feed to Open, click Insert. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 264 In the Instruction Box: • Set Breakpoint to 0.00 min. • In the Instructions: list, select Other and Block. In the Parameters for field, select the Block →DELAY_3_SEC block. Click Insert. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 265 • Set Hysteresis to an appropriate value, for instance 0.5 l. • Set Tank Fill to Enabled. Click Insert. Instruction: Transfer feed setup Step Action In the Text Instructions tab, select the Base →SameAsMain instruction of the Block →PARAMETERS_CONCENTRATION block. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 266 In the Instruction Box: • Set Breakpoint to 0.00 min. • In the Instructions: list, select Recirc and ConcFac_FedBatch_On. Click Insert. Progress review The Method Editor should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 267 This block ensures that a stable Delta P value is reached. Step Action In the Text Instructions tab, select the Block →PARAMETERS_CONCEN- TRATION instruction of the Phase →Fed Batch Concentration phase. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 268 • Set Breakpoint to 0.00 min. • In the Instructions: list, select Specials and DeltaP_WatchPar. In the Parameters for field: • Set BaseLine to for instance 0.1 bar. • Set MinPeak to 0.00 bar. Click Insert. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 269 Nested block: Trans membrane pressure (TMP) setup This block sets the TMP value. Step Action In the Text Instructions tab, select the Block →STABLE_DELTA_P instruc- tion of the Phase →Fed Batch Concentration phase. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 270 In the Text Instructions tab, select the Base →SameAsMain instruction of the Block →TMP_START_STOP block. In the Instruction Box: • Set Breakpoint to 0.00 min. • In the Instructions: list, select Watch and Watch. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 271 In the Instruction Box, set Breakpoint to a suitable time, for instance 60.00 min. Click Change. Progress review If all the steps described above have been performed, the Method Editor window should now look something like this. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 272 Phase 3: Fed Batch Concentration If any step appears to be missing, misplaced or carries incorrect values, use the various editing methods available in the Method Editor to correct the errors. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 273: Saving The Method
C Create a method with UNICORN 6 and above C.2 An example of using the Method Editor Saving the method Saving the method To save the method, click the discette icon or select File →Save in the menu. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 274: Appendix D: Pid Control And Feedback Tuning
This chapter provides information to enable users to set up a satisfactory control behaviour of the UniFlux by being able to understand and use the PID control functions provided by UNICORN. In this chapter Section See page Introduction Using PID control More information UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 275: Introduction
• Too large P-component will make the system unstable. • Too small P-component will make the regulator slow and prevent it from elimi- nating the error entirely. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 276 • Too small D-component may cause for the system to respond slowly to errors and possiblly oscillate. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 277: Using Pid Control
There are two ways to apply the feedback tuning instructions: • In the Method Editor module. • In the System Control module, reached with the commands Manual or System Settings. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 278: More Information
When the regulation is too fast, for example noisy curves, it may be necessary to opti- mize the parameters in the opposite way, i.e., increase I parameter and decrease P parameter. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 279 The system will be unable to eliminate errors completely. Large The system will overshoot initally when regulating for errors. Small The system will be prevented from increasing the P- component enough to achieve fast response without resulting oscillations. UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 280: Index
154 Example, 248 external surfaces, 155 create a method, 248 general, 154 Explosive environment, 15 Cleaning performance, 156 precautions, 15 extended, 156 External connections, 86 Component descriptions, flow- chart, 89 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 281 Method, saving, 236 Instruction Box, 244 Modify an instruction, 248 Instructions, 245 More information, 201 method editor, 245 Multiple inlets option, 55 Instructions, method editor, 208 NFF, 124, 125 reasons, 124 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 282 Power supply, 19, 117 precautions, 19 Prerequisites, 8 Tank level watch, 260 Pressure alarms setting, 252 Temperature sensors, 83 Pressure alarms, setting in Text instructions, 244 method, 217 TMP alarm, 255 Pressure sensors, 80 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 283 102 tools required, 101 visual inspection, 102 User allowed actions, 161 User console, 53 UV monitor option, 56 UV sensors, 82 Variables, 246 instruction box, 246 Weights, 180 Wetted materials, 201 UniFlux 30 and UniFlux 120 Operating Instructions 28981012 AE...
Page 284 Standard Software End-User License Agreement is available on request. All goods and services are sold subject to the terms and conditions of sale of the supplying company operating within the Cytiva business. A copy of those terms and conditions is available on request. Contact your local Cytiva representative for the most current information.

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