Low-Pressure Gradient System - Hitachi PRIMAIDE 1440 Instruction Manual

1.2 Low-Pressure Gradient System

Primaide
Organizer
Primaide 1110
In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
Mobile phase Wash Solution
Pump
Degassing
Proportioning valve
device
置
Fig. 1-2 Low Gradient System
(1) Each of plural eluents is fed from the degassing device to
the low-pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-
specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
Mixer
Column
Four-way joint
1 - 2
Primaide 1440
Fluorescence
Detector
Detector cell
Primaide 1310
Column Oven
Primaide 1210
Autosampler
Drain