Preparation; Degassing And Removing Dust From Mobile Phase; Degassing Mobile Phase - Hitachi PRIMAIDE 1440 Instruction Manual

3.7 Preparation

3.7.1 Degassing and removing dust from mobile phase

3.7.2 Degassing mobile phase

NOTE: When using an ultrasonic cleaner etc., follow the
instruction manual for it.
(1) Degas mobile phase before use without fail.
Oxygen gas and nitrogen gas contained in mobile phase
cause the following when used under high pressure.
(a) Change in quality of mobile phase sample
(b) Loss of column efficiency caused by bubbles
(c) Noise of detector caused by bubbles
(d) Variation of pump pressure
(e) Reduced precision of gradient mixing ratio
(2) For degassing mobile phase, two methods are available,
Degassing unit and Manual. Degassing method is
explained in 3.7.2.
(3) Use mobile phase without dust.
Dust in mobile phase causes trouble in pumping and the
pressure may vary.
There are various ways of degassing.
Perform degassing suitable for the purpose of use.
(1) Example of Degassing by Ultrasonic Waves (when mobile
phase is organic solvent)
Example : Methanol 100%
Preparation : Ultrasonic cleaner (put water in the bath
3 - 60
Mixture of methanol 80% and acetonitrile
20%
without fail)