Sigma 2-7 Operating Manual
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Sigma 2-7 Operating Manual

Laboratory centrifuge
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Translation of the original operating manual
sb
07/2015
Rev. 1.12 of 16/07/2019
Sigma 2-7, Sigma 2-7 Cyto;Laboratory centrifuge;Sigma 2-7 IVD; ;
Laboratory centrifuge
part no. 0702802
Sigma 2-7, Sigma 2-7 Cyto
Sigma 2-7 IVD
from serial no. 157336
Laboratory centrifuge
Operating Manual
Please retain for later use!

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  • Page 1 Translation of the original operating manual 07/2015 Rev. 1.12 of 16/07/2019 Sigma 2-7, Sigma 2-7 Cyto;Laboratory centrifuge;Sigma 2-7 IVD; ; Laboratory centrifuge part no. 0702802 Sigma 2-7, Sigma 2-7 Cyto Sigma 2-7 IVD from serial no. 157336 Laboratory centrifuge Operating Manual...
  • Page 2 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 2 / 68 Translation of the original operating manual, part no. 0702802...
  • Page 3 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD © Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel.: +49 (0) 5522 / 5007-0 Fax: +49 (0) 5522 / 5007-12 Web: www.sigma-zentrifugen.de...
  • Page 4 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 4 / 68 Translation of the original operating manual, part no. 0702802...

  • Page 5: Table Of Contents

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................
  • Page 6 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Table of contents Storage and transport ....................... 23 Dimensions and weight ....................23 Storage conditions ......................23 Notes on transport ......................23 Packaging ........................24 Transport safety device ....................24 Set-up and connection ......................
  • Page 7 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Table of contents Malfunctions and error correction ..................37 General malfunctions ...................... 37 7.1.1 Emergency lid release ....................38 Table of error codes ......................39 Service contact ....................... 40 Maintenance and service ....................
  • Page 8 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Table of contents Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 8 / 68 Translation of the original operating manual, part no. 0702802...

  • Page 9: General Information

    • compliance with the inspection and maintenance instructions. Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from non-compliance.  Warranty and liability The warranty and liability are subject to our "General Conditions"...

  • Page 10: Copyright

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 1 General information  Copyright The copyright concerning the operating manual remains with Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be •...

  • Page 11: Layout And Mode Of Operation

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 2 Layout and mode of operation  Layout and mode of operation  Layout of the centrifuge 2.1.1 Functional and operating elements 1 2 Display 3 User interface (see chapter 6.3.1 -...

  • Page 12: Name Plate

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 2 Layout and mode of operation 2.1.2 Name plate 1 Manufacturer 2 Power consumption 3 Max. speed 4 Max. kinetic energy 5 Serial number 6 Part number 7...

  • Page 13: Mode Of Operation

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 2 Layout and mode of operation  Mode of operation  2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components.

  • Page 14: Speed, Radius, And Relative Centrifugal Force

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 2 Layout and mode of operation  2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.

  • Page 15: Safety

    Laboratory centrifuge Sigma 2-7 IVD 3 Safety  Safety  Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma: Dangerous voltage On (Power) Hot surface Off (Power) Name plate (see chapter Caution! Risk of bruising 2.1.2 - "Name plate")

  • Page 16: Explanation Of The Symbols And Notes

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced. NOTE Not all of the symbols/labels are used for this centrifuge type.

  • Page 17: Responsibility Of The Operator

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety  Responsibility of the operator The operator undertakes to authorise only trained, specialised personnel to work on the centrifuge (see chapter 3.4 - "Operating personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.

  • Page 18: Safety Instructions

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety  Safety instructions  3.6.1 Electrical safety To reduce the risk of electrical shock, the centrifuge uses a three-wire electrical cord and plug to connect the equipment to earth-ground. To preserve this safety feature: •...

  • Page 19: Fire Prevention

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety • Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, and adapters) in terms of visible structural changes.

  • Page 20: Safety Instructions For Centrifugation

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety  3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection").

  • Page 21: Safety Of Rotors And Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 3 Safety 3.6.7 Safety of rotors and accessories 3.6.7.1 Service life The rotors and accessories have a limited service life. Perform regular checks (at least once per month) for safety reasons! •...

  • Page 22: Standstill Monitoring System

    3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact").  Measures in the event of hazards and accidents •...

  • Page 23: Storage And Transport

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 4 Storage and transport  Storage and transport Dimensions and weight Sigma 2-7, Sigma 2-7 Cyto, Sigma 2-7 IVD Height: 293 mm Height with open lid: 643 mm Width:...

  • Page 24: Packaging

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 4 Storage and transport Packaging The centrifuge is packaged in a cardboard box. • Open the box. • Take out the box containing the accessories. • Remove the upper foam element.

  • Page 25: Set-Up And Connection

    DANGER Sigma centrifuges are units of protection class I. The centrifuges of this model series have a three-wire power cord with an IEC C13 connector. The removable power cord must not be replaced with a power cord of...

  • Page 26: Using The Centrifuge

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge  Using the centrifuge  Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection").

  • Page 27: Installation Of Rotors And Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge 6.2.2 Installation of rotors and accessories 6.2.2.1 Installation of a rotor • Open the centrifuge lid by pressing the lid key. • Loosen the rotor tie-down screw by turning it anti-clockwise, but do not remove it.

  • Page 28: Installation Of Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge  6.2.2.2 Installation of accessories • Only use inserts that are suitable for the rotor. • All buckets of the swing-out rotor need to be installed when spinning.

  • Page 29: Adapters

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge Pay attention to the marking of the centrifuge (see the illustration below)! Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced.

  • Page 30: Control System "Spincontrol Basic

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge Control System "Spincontrol Basic" 6.3.1 User interface 1 Display 2 Set key 3 Program key 4 Arrow keys 5 Lid key 6 Start/Stop key 7...

  • Page 31: Starting A Centrifugation Run

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge 6.3.3 Starting a centrifugation run The centrifuge is ready for operation when the mains power switch is on and the lid is closed. •...

  • Page 32: Runtime

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge 6.3.7 Runtime The preselected total runtime is displayed in the lower line of the display. During centrifugation, the remaining runtime is displayed. The runtime of the centrifuge can be set at one-second-intervals up to 99 minutes and 59 seconds.

  • Page 33: Rotor Selection

    11037 11071 with 13299 11071 with 13296 12072 12073 11123 with 13223 (Not certified for Sigma 2-7 IVD!) Sigma 2-7 Cyto Code Rotor / bucket 11260 cyto rotor, closed, RESOSPIN with stainless-steel clip 13260 11261 cyto rotor, closed, SHANDON with stainless-steel clip 13261...

  • Page 34: Softstart And Softstop Function

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge 6.3.9 Softstart and softstop function The softstart function is used to extend the acceleration time, whereas the softstop function is used to extend the deceleration time. The current combination is shown on the display.

  • Page 35: 6.3.12 Sound Signal

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge The selected delay is activated. During the set start delay, the word "clot" is displayed and the blue LED flashes slowly. When the actual centrifugation process starts, the LED lights continuously.

  • Page 36: 6.3.15 Programs

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 6 Using the centrifuge 6.3.15 Programs Program isare used to save or load certain recurrent settings of the centrifuge. 10 different programs can be saved and called up.

  • Page 37: Malfunctions And Error Correction

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 7 Malfunctions and error correction  Malfunctions and error correction General malfunctions Malfunctions are indicated by error messages with a number in the speed/RCF display. In addition, the blue LED flashes rapidly.

  • Page 38: Emergency Lid Release

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.

  • Page 39: Table Of Error Codes

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 7 Malfunctions and error correction  Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to •...

  • Page 40: Service Contact

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 7 Malfunctions and error correction  Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel.

  • Page 41: Maintenance And Service

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.

  • Page 42: Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at...

  • Page 43: Rotors, Buckets And Carriers

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  8.1.3 Rotors, buckets and carriers Rotors, buckets and carriers are produced with the highest precision, in order to withstand the permanent high stress from high gravitational fields.

  • Page 44: Sterilisation And Disinfection Of The Rotor Chamber And Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  Sterilisation and disinfection of the rotor chamber and accessories • Use commercially-available disinfectants such as, for example,    (available at chemist’s shops or...

  • Page 45: 8.2.1 Autoclaving

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.

  • Page 46: Service

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. Only qualified specialist personnel is authorised to perform this service work.

  • Page 47: Return Of Defective Centrifuges Or Parts

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service  Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer.
  • Page 48 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 8 Maintenance and service The defective part/unit must be packaged in a transport-safe manner. Please use the original packaging for the unit, if at all possible. If the product is dispatched to us in unsuitable packaging, you will be charged the cost for returning it to you in new packaging.

  • Page 49: Disposal

    Disposal  Disposal of the centrifuge In accordance with the directive 2002/96/EC, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...

  • Page 50: 10 Technical Data

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 10 Technical data  10 Technical data Manufacturer: Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Sigma (Germany) Type: Sigma 2-7 Sigma 2-7 IVD Order no.:...

  • Page 51: Ambient Conditions

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 10 Technical data Manufacturer: Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: 2-7 Cyto Order no.: 10228 Connection requirements Electrical connection: see name plate...

  • Page 52: Appendix

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix  11 Appendix  11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below.

  • Page 53: Speed-Gravitational-Field-Diagram

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix  11.2 Speed-gravitational-field-diagram Fig. 14: Speed-gravitational-field-diagram Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 53 / 68 Translation of the original operating manual, part no. 0702802...

  • Page 54: Table Of The Service Life Of Rotors And Accessories

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix  11.3 Table of the service life of rotors and accessories • If no other data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10 years.

  • Page 55: Resistance Data

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix  11.4 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant...
  • Page 56 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid...
  • Page 57 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl...
  • Page 58 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide...

  • Page 59: Ec Declaration Of Conformity

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix 11.5 EC declaration of conformity Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 59 / 68 Translation of the original operating manual, part no. 0702802...
  • Page 60 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 60 / 68 Translation of the original operating manual, part no. 0702802...
  • Page 61 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 61 / 68 Translation of the original operating manual, part no. 0702802...
  • Page 62 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 62 / 68 Translation of the original operating manual, part no. 0702802...

  • Page 63: Declaration Of Conformity - China Rohs 2

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix Declaration of conformity – China RoHS 2 11.6 Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 63 / 68 Translation of the original operating manual, part no. 0702802...
  • Page 64 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD 11 Appendix Version 07/2015, Rev. 1.12 of 16/07/2019 • sb 64 / 68 Translation of the original operating manual, part no. 0702802...

  • Page 65: 12 Index

    Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Index 12 Index Cleaning agents ......... 44 Cleaning the bores of angle rotors ..... 42 Acceleration time Cleaning the centrifuge ......41 - see also the softstart and softstop function Closing the lid ........
  • Page 66 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Index Documentation ........... 10 Interrupting a centrifugation run ....31 Interrupting a deceleration process .... 31 IP code ..........50, 51 Earth conductor check ....... 22 EC declaration of conformity ....10, 59 EEPROM error ...........
  • Page 67 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Index Operational safety ........42 Safety distance ......18, 20, 25 Overseas shipping ........23 Safety instructions ......9, 27, 29 Safety instructions for centrifugation ..20 Safety of rotors and accessories ....21 Packaging ..........
  • Page 68 Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto Laboratory centrifuge Sigma 2-7 IVD Index Table of rotors and accessories with a different service life ......21, 54 Units of protection class I ......25 Technical data ........50, 51 User interface ..........30 Technical documentation ......

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