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Translation of the original operating manual 02/2013 Rev. 1.19 of 25/04/2019 Sigma 2-16KHL; ; part no. 0700802 Sigma 2-16KHL from serial no. 147260 Refrigerated Centrifuge with Heating System Operating Manual Please retain for later use!
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 2 / 80 Translation of the original operating manual, part no. 0700802...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 4 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Table of contents Storage and transport ....................... 24 Dimensions and weight ....................24 Storage conditions ......................24 Notes on transport ......................24 Packaging ........................25 Transport safety device ....................25 Set-up and connection ...................... 26 Installation site ........................
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Table of contents Malfunctions and error correction ..................47 General malfunctions ...................... 47 7.1.1 Emergency lid release ....................48 Table of error codes ......................49 Service contact ....................... 50 Maintenance and service ....................51 Maintenance ........................
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Table of contents Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 8 / 80 Translation of the original operating manual, part no. 0700802...
They are solely intended for this purpose. Any other use beyond this area of application is regarded as improper use. Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 1 General information Copyright The copyright concerning the operating manual remains with Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be •...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 2 Layout and mode of operation Layout and mode of operation Layout of the centrifuge 2.1.1 Functional and operating elements 1 2 User interface (see chapter 6.3.1 - "User interface") 3...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 2 Layout and mode of operation 2.1.2 Name plate 1 Manufacturer 2 Power consumption 3 Max. speed 4 Max. kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 2 Layout and mode of operation Mode of operation 2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 2 Layout and mode of operation 2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety Safety Marking of the unit The following symbols are used for all types of centrifuges manufactured by Sigma: Dangerous voltage On (Power) Hot surface Off (Power) Name plate (see chapter 2.1.2 Caution! Risk of bruising - "Name plate")
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety Explanation of the symbols and notes In this operating manual, the following names and symbols to indicate hazards are used: This symbol stands for a direct hazard to the life and health of persons.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety Responsibility of the operator The operator is responsible for authorising only qualified personnel to work on the centrifuge (see chapter 3.4 - "Operating personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety Safety instructions 3.6.1 Electrical safety To reduce the risk of electrical shock, the centrifuge uses a three-wire electrical cord and plug to connect the equipment to earth-ground. To preserve this safety feature: •...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety • Prior to any start-up, check the centrifuge, rotor, and accessories for signs of damage that can be discerned from the outside. Special attention must be paid to all of the rubber parts (e.g. motor cover, lid seal, and adapters) in terms of visible structural changes.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection").
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety 3.6.7 Safety of rotors and accessories 3.6.7.1 Service life The rotors and accessories have a limited service life. Perform regular checks (at least once per month) for safety reasons! • Pay special attention to changes, such as corrosion, cracks, material abrasion, etc.
(see chapter 7.2 - "Table of error codes"). 3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact"). 3.7.5 Imbalance monitoring system The indication "Imbalance"...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 3 Safety Measures in the event of hazards and accidents • If an emergency arises, switch off the centrifuge immediately! • If in doubt, call the emergency doctor! DANGER Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 4 Storage and transport Storage and transport Dimensions and weight Sigma 2-16KHL Height: 310 mm Height with open lid: 705 mm Width: 550 mm Depth: 570 mm Weight: 60 kg ...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 4 Storage and transport Packaging The centrifuge is packaged in a threepart slip-lid box. • Take off the lid. • Remove the box containing the accessories and the packaging material. • Remove the ring of the slip-lid box.
DANGER Sigma centrifuges are units of protection class I. The centrifuges of this model series have a three-wire power cord with an IEC C13 connector. The removable power cord must not be replaced with a power cord of...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 5 Set-up and connection 5.2.2 Customer-provided fuses Typically, the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer. Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 27 / 80 Translation of the original operating manual, part no.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Using the centrifuge Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection").
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge When using rotors for microtiter plate formats: Ensure that the plate holders are inserted together with the plates into the buckets. CAUTION The lid screw serves for the fastening of the lid onto the rotor only, not for the fastening of the rotor onto the motor shaft.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Removal of a rotor • Unscrew the rotor tie-down screw (counter-clockwise) and remove the rotor with the lid. • Open the lid by turning it counter-clockwise and remove the lid.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Operation • Fill the capillary tubes with blood and seal them at one end with putty or by fusion. • Place the capillary tubes into the recesses of the rotor with the sealed end against the rubber ring.
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Centrifugation with low capacity • Install the sample vessels in a rotationally symmetrical manner so that the buckets and their suspensions are loaded evenly. • It is not permissible to load angle rotors on only one axis.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.2.2.5 Adapters In order to ensure easy handling, even if vessels of various sizes are used, carrier systems were developed. • Load the opposite adapters with the same number of vessels and with the same weights in order to avoid imbalance.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Control system "Spincontrol L" 6.3.1 User interface The centrifuge is operated via three buttons with integrated light-emitting diodes and one function knob. The display is divided into several different fields.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2 Manual mode 6.3.2.1 Starting a centrifugation run The centrifuge is ready for operation when the start key is illuminated. • Press the start key in order to start a centrifugation run.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2.1 Speed / relative centrifugal force (RCF) The set speed of the centrifuge is displayed in the upper area of the Speed/RCF field. The actual value is displayed right below. The speed is...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Runtime as of the set speed If the runtime is to be counted as of the moment when the set speed is reached, the symbol (see the illustration) behind the set speed value must be activated: •...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Short run A short run can be started if no run is active. • Keep the start key pressed during the short run. During the short run, the centrifuge accelerates with the acceleration curve 9 (maximum) until the maximum permissible speed of the rotor is reached.
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Program "Rapid Temp" The centrifuge has a special "Rapid Temp" program that brings the centrifuge rapidly to a specified temperature under defined conditions. • Select the program with the aid of the menu item "run Prog" (see chapter 6.3.3.2 - "Loading a program").
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2.4 Rotor selection This field shows the rotor that is currently being used. The rotor selection can only be changed when the centrifuge is at a standstill.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2.5 Acceleration and deceleration curves Acceleration This function is used to select an acceleration curve. The system offers 10 fixed, programmed acceleration curves (curves 0-9). Brake This function is used in order to select a curve that decelerates the centrifuge to a standstill.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2.7 Sound signal ("Buzzer") This function is used to set an acoustic warning signal that sounds at the end of the centrifugation run and also in the event of an imbalance or error message.
3.6.7.1 - "Service life "). WARNING The cycle display will be reset after the rotor and buckets have been replaced by the service department of Sigma Laborzentrifugen GmbH (see chapter 7.3 - "Service contact"). Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 43 / 80 Translation of the original operating manual, part no.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.2.10 Input lock In order to prevent the centrifuge from being manipulated by unauthorised persons, inputs can be disabled via the menu. Inputs via the keypad, i.e. for starting or stopping a centrifugation run or for opening the lid, are enabled.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge 6.3.3.1 Saving a program This function is only available if the centrifuge is at a standstill. NOTE • Enter the parameters that are to be included in the program.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 6 Using the centrifuge Switching the centrifuge off • Open the centrifuge when it is not in use so moisture can evaporate. • Switch the centrifuge off by pressing the mains power switch.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 7 Malfunctions and error correction Malfunctions and error correction General malfunctions Error messages are displayed as "Error" followed by a code number. If the acoustic signal is activated, it sounds when the error message is displayed.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 7 Malfunctions and error correction Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to •...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 7 Malfunctions and error correction Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel. +49 (0) 55 22 / 50 07-44 44 E-mail: support.lab@sigma-zentrifugen.de...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service 8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration system) In order to cool the refrigerant that is compressed by the refrigeration unit, centrifuges with an air-cooled refrigeration system use a lamellar condenser.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service 8.1.2.1 Plastic accessories The chemical resistance of plastic decreases with rising temperatures (see chapter 11.5 - "Resistance data"). • If solvents, acids, or alkaline solutions have been used, clean the plastic accessories thoroughly.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service 8.1.5 Load-bearing bolts Plastic bucket The load-bearing bolts of the rotor must not be greased if plastic buckets are used! NOTE Aluminium buckets Only greased load-bearing bolts ensure a uniform swing-out of the buckets and, therefore, the smooth operation of the centrifuge.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service 8.1.6 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. Only qualified specialist personnel is authorised to perform this service work.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer.
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 8 Maintenance and service The defective part/unit must be packaged in a transport-safe manner. Please use the original packaging for the unit, if at all possible. If the product is dispatched to us in unsuitable packaging, you will be charged the cost for returning it to you in new packaging.
Disposal Disposal of the centrifuge In accordance with the directive 2002/96/EC, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 10 Technical data 10 Technical data Manufacturer Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Type: 2-16KHL Connection requirements Electr. connection: see name plate Protection class: IP code: Connected load (kVA): 1.01...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 10 Technical data 10.1 Ambient conditions • The figures are valid for an ambient temperature of +23°C and a nominal voltage 10 %. The minimum temperature is ≤ +4°C and depends on the rotor type, speed, and ambient temperature.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11 Appendix 11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. 11.1.1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below.
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix Fig. 32: Microhaematocrit rotor Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 64 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11.2 Speed-gravitational-field-diagram Fig. 33: Speed-gravitational-field-diagram Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 65 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11.3 Acceleration and deceleration curves Linear curves are numbered in the direction of increasing acceleration (from right to left). The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11.4 Table of the service life of rotors and accessories • If no other data concerning the service life are engraved on the rotor or accessory, rotors and buckets must be checked by the manufacturer after 10 years.
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11.5 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide saturated...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate CuSO x 5H...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate Methyl alcohol...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide NaOH Sodium sulfate...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 72 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix 11.6 EC declaration of conformity Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 73 / 80 Translation of the original operating manual, part no. 0700802...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 74 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix Declaration of conformity – China RoHS 2 11.7 Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 75 / 80 Translation of the original operating manual, part no. 0700802...
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL 11 Appendix Version 02/2013, Rev. 1.19 of 25/04/2019 • sb 76 / 80 Translation of the original operating manual, part no. 0700802...
Refrigerated Centrifuge with Heating System Sigma 2-16KHL Index 12 Index Changes in colour ........56 Changes in structure ........56 Acceleration ..........41 Chemical and biological safety ....19 Acceleration curves ......41, 66 Chemical resistance of plastic ....53 Accessories, cleaning and care ....
Refrigerated Centrifuge with Heating System Sigma 2-16KHL Index Different service life of rotors and accessories Imbalance monitoring system ....22 ............21, 67 Importance of the operating manual ..... 9 Dimensions ..........24 Important information ......... 16 Direct hazard to the life and health ..... 16 Improper loading ........
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Index Maximum number of cycles ....... 43 Protection class ........26, 61 Maximum speed for tubes ......33 Measures in the event of hazards and Quick stop ..........35 accidents ..........23 Quitting the modification mode ....35 Mechanical safety ........
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Refrigerated Centrifuge with Heating System Sigma 2-16KHL Index Saving a program........45 Scope of supply ......... 10 Table of error codes ........49 Selection, display, and modification of data 35 Table of rotors and accessories with a Serial number ........ 12, 50, 57 different service life ......
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