Leica VT1000 S Instructions For Use Manual
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Leica VT1000 S Instructions For Use Manual

Vibrating-blade microtome
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Instructions for Use
VT1000 S
Vibrating-blade microtome
Leica VT1000 S, V 2.3 , English - 09/2018
Order No. 14 0472 80101 RevK
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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  • Page 1 Instructions for Use VT1000 S Vibrating-blade microtome Leica VT1000 S, V 2.3 , English - 09/2018 Order No. 14 0472 80101 RevK Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 Leica reserves the right to change technical spec- following thorough investigation in this field. We ifications as well as manufacturing processes are under no obligation to update the present without prior notice.

  • Page 4: Table Of Contents

    The operating elements and their function - VT1000 S ............15 Adjusting the amplitude ......................19 Working with the VT1000 S on a daily basis................20 Routine daily maintenance and switching off the instrument - VT1000 S ......23 Malfunctions: Meaning and Troubleshooting ............24 Cleaning and Maintenance ..................29 Cleaning the instrument......................

  • Page 5: Important Information

    ElektroG is the law on the bringing into circulation, return and environmentally compatible disposal of electrical and electronic equipment. Caution! Follow the accompanying documentation! The CE labeling shows that the product corresponds to one or more applicable European directives. Leica VT1000 S - Vibrating-blade microtome...
  • Page 6 Intended use/improper use The VT1000 S is used for sectioning in the fields of medicine, biology and industry, and is espe- cially designed for sectioning fixed or unfixed fresh tissue in a buffer solution. The VT1000 S may be used for research purposes only. Sections made using the VT1000 S...

  • Page 7: Safety

    Operating the instrument safely is, above all, the responsibility of the owner, as well as the designated personnel who operate, service or clean the instrument. To ensure trouble-free operation of the instrument, make sure to comply with the following instructions and warnings. Leica VT1000 S - Vibrating-blade microtome...

  • Page 8: Safety Instructions For Handling The Instrument

    Safety Safety instructions for handling the instrument Proper handling Danger Be sure to handle knives and blades very cau- Caution: Extremely sharp blades pose tiously! risk of injury when touched! Never touch the cutting edge of knives and blades! Do not leave knives, blades and bladed knife Caution: Fresh tissue poses risk of holders unprotected.

  • Page 9: Instrument Characteristics

    L x W x H .......................480 mm x 360 mm x 200 mm Height with magnifier support ........................ 285 mm Weight: (without magnifier support) ........................17 kg (magnifier support only) ..........................2 kg (total) ................................19 kg Leica VT1000 S - Vibrating-blade microtome...

  • Page 10: General Overview - Vt1000 S

    Instrument characteristics General overview - VT1000 S Module LED Hi-Power spots, 2-arm (optional) Module Hi-Power spot, LED 1000 (optional) Magnifier support Magnifier EMERGENCY STOP switch (not visible here) Fixture for Cutting head magnifier support Knife holder Buffer tray S Control panel Fig.
  • Page 11 Fig. 3 Pause button - stops sectioning process Connecting socket for power cord Fixing device for drain tube Power switch EMERGENCY STOP button Connection for foot switch, 9-pole Fig. 4 Leica VT1000 S - Vibrating-blade microtome...

  • Page 12: Installation

    Language CD 14 0472 80200) ..........14 0472 80001 VT1000 S complete configuration ..........14 0472 35613 - VT1000 S basic instrument ............14 0472 35612 - 3 Specimen discs S, non orientable ........14 0463 27404 - Buffer tray S................14 0462 30132 - 5 Countersunk screw, M 5 x 8 ..........

  • Page 13: Unpacking And Setting Up The Instrument

    Tilt the instrument including the baseplate (Fig. 8) - hold the instrument with one hand on the recess (8) for the buffer tray! NEVER lift or hold it by the cutting head (7)! Unscrew the screw (6) using the size 8 Allen key provided and remove the base plate. Leica VT1000 S - Vibrating-blade microtome...
  • Page 14 Installation 5. Using both hands at the sides (Fig. 9), grasp the bottom of the instrument and carefully place it on a suitable laboratory table. Compare with the attached packing list to make sure the delivery is complete. Fig. 9 Assembling the drain tube •...

  • Page 15: Operation

    • Room temperature consistently between +5 °C and +40 °C • Avoid vibrations, direct sunlight, and large temperature fluctuations! The instrument MUST be connected to a grounded power socket. Use only a provided power cable that is intended for the local power supply. Leica VT1000 S - Vibrating-blade microtome...

  • Page 16: Setting Up The Instrument

    The instrument MUST be set up so that the power plug and switch are free and easily accessible at all times! The Leica VT1000 S is equipped with a autoranging power supply to cover voltages from 100 V to 240 V.

  • Page 17: The Operating Elements And Their Function - Vt1000 S

    Operation The operating elements and their function - VT1000 S Caution: Practice working with the controls without a blade holder inserted. Only insert the knife holder when you are completely familiar with all control functions. Locking lever SPEED 10-speed rotating potentiometer with scale...
  • Page 18 Operation Function: • When the V-Max button is activated in manual mode (LED on - red light) and the REV/FORW button is pressed, the knife moves towards the speci- men at maximum speed. Button with LED • When the START button is pressed, the LED in the V-Max button is extinguished.
  • Page 19 µm"= section thickness totalizing ∑ "µm" = section thickness Function of the CLR button in section thickness totalizing mode: Sets the value indicated in the section thickness totalizing mode ( µm) to ∑ zero. Leica VT1000 S - Vibrating-blade microtome...
  • Page 20 Once the switch is unlocked, the buffer tray is automatically raised until both signals switch off. • To select the retraction thickness, to deactivate retraction or to set the volume of the VT1000 S warning signal, press the following function key combinations: Volume adjustment: •...

  • Page 21: Adjusting The Amplitude

    • Slide the amplitude clamping screw to the desired amplitude position and retighten. When adjusting the amplitude setting, do not remove the clamping screw, simply loosen it. The instrument is shipped with the amplitude set to 0.6 mm. Leica VT1000 S - Vibrating-blade microtome...

  • Page 22: Working With The Vt1000 S On A Daily Basis

    Operation Working with the VT1000 S on a daily basis • Mount the buffer tray (1) onto the bolt (2) inside the cooling bath (3). • Secure the buffer tray by relocating the clamping lever (4) to the right (in the direction of the arrow).
  • Page 23 • Use the adjusting lever (2) to select the desired clearance angle. • Secure the selected clearance angle by tightening the two screws (1). The Leica VT1000 S does not require the readjustment of the clear- ance angle every time you change the blade. Make an adjustment only if required by an application for technical reasons (e.g.
  • Page 24 Operation • Fix the knife holder (1) with the knife holder clamping screw (2). • Use the REV/FORW rocker button to place the blade edge right behind the rear edge (from user's view) of the specimen. • Pull the UP/DOWN rocker button into the UP direction and keep it in the UP position until the specimen surface is shortly below the level of the blade edge (see arrow (3, Fig.

  • Page 25: Routine Daily Maintenance And Switching Off The Instrument - Vt1000 S

    Operation Routine daily maintenance and switching off the instrument - VT1000 S After all daily procedures have been finished, perform the following: • Switch off the main switch at the back of the instrument. • Place the magnifier cover on the magnifier.

  • Page 26: Malfunctions: Meaning And Troubleshooting

    Malfunctions: Meaning and Troubleshooting Error messages/symptoms Sources of error Troubleshooting - Clearance angle adjustment: - Collision of knife and specimen - If a clearance angle wider than - Lower the specimen disc suf- disc. 5° is selected, specimen disc ficiently to prevent collision. and knife edge can potentially collide with each other.
  • Page 27 - When pressing any key, the instrument gives an audible warning signal. - In case the EMERGENCY STOP function has been activated, the instrument will remain inopera- tional when pressing the foot switch. - The indication SP is displayed. Leica VT1000 S - Vibrating-blade microtome...
  • Page 28 Malfunctions: Meaning and Troubleshooting Error messages/symptoms Sources of error Troubleshooting - Audible warning signal. - Button(s) jammed or defective. - Push the button several times to - Error code E0.1xx is displayed. - Locking function /REV or REV/ unlock; FORW button defective. have defective button replaced by the Technical Service.
  • Page 29 (999 µm). - Audible warning signal. - The warning signal will cease (When operating the instrument automatically after the initializa- for the first time or after the tion phase. E-EPROM has been exchanged.) Leica VT1000 S - Vibrating-blade microtome...
  • Page 30 Malfunctions: Meaning and Troubleshooting Error messages/symptoms Sources of error Troubleshooting - A clattering sound can be heard. - The visible clamping screws - Retighten the loose clamping have become loose during sec- screws. tioning. If the clattering sound does These symptoms may occur not cease once the clamping from time to time and are un- screws have been retight-...

  • Page 31: Cleaning And Maintenance

    Cleaning the knife When cleaning the knife/blade, always wipe from the knife or blade back towards the cutting edge, NEVER wipe in the opposite direction - risk of injury! Clean using an alcohol-based solution or acetone. Leica VT1000 S - Vibrating-blade microtome...

  • Page 32: Changing The Fuse

    Cleaning and maintenance Changing the fuse Before changing a fuse, always switch off the instrument first and remove the instrument cable completely. The instrument must have cooled down and the paraffin tank must be empty. When changing a fuse, do NOT use any fuses other than the spare fuses supplied with the instru- ment.

  • Page 33: Ordering Information: Spare Parts, Accessory, Consumables

    100 V/50/60 Hz ................... 14 0481 48439 115 V/50 Hz ..................14 0481 48437 230 V/50-60 Hz .................. 14 0481 48436 230 V/60 Hz ..................14 0481 48438 Antifrogen N ..................14 0481 45443 Leica VT1000 S - Vibrating-blade microtome...

  • Page 34: Foot Switch

    Ordering Information: Spare Parts, Accessory, Consumables Foot switch Foot switch • The foot switch is an optional accessory which can be used instead of the START/STOP button. Order No......14 0463 27415 Fig. 34 Buffer tray 8.2.1 Double-walled buffer tray S When using the double-walled buffer tray, the flow cooler must be installed according to the assembly instructions prior to working with specimens.

  • Page 35: Magnifier, Led Illumination

    Order No........14 6000 04826 Module Hi-Power spot, LED 1000 • Serves as a light source for the module LED Hi-Power spots, 2-arm. Order No........14 6000 04825 Fig. 37 Fig. 38 Leica VT1000 S - Vibrating-blade microtome...

  • Page 36: Julabo Recirculating Cooler/Chiller Fl300

    Ordering information: Spare Parts, Accessory, Consumables Julabo recirculating cooler/chiller FL300 Recirculating cooler/chiller for connection to the double-walled buffer tray in the Leica VT1000 S and VT1200/VT1200 S. Selectable temperature range: –20 °C to +40 °C. Recommended cooling medium: Antifrogen N (14 0481 45443)

  • Page 37: Warranty And Service

    Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.

  • Page 38: Decontamination Confirmation

    Decontamination Confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data.
  • Page 40 Leica Biosystems Nussloch GmbH Heidelberger Str. 17-19 D- 69226 Nussloch Phone: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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