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Leica Aperio ImageScope DX User Manual

Leica Aperio ImageScope DX User Manual

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Aperio ImageScope DX
User's Guide
MAN-0352, Revision A | 20 May 2019

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  • Page 1 Aperio ImageScope DX User’s Guide MAN-0352, Revision A | 20 May 2019...
  • Page 2 Copyright © 2019 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica Microsystems IR GmbH. Aperio is a trademark of the Leica Biosystems group of companies in the USA and optionally in other countries. Other logos, product and/or company names might be trademarks of their respective owners.
  • Page 3 Tel: 0080052700527 (toll free) In-country Tel: +31 70 413 21 00 Danmark: Email: support.nl@leicabiosystems.com Tel: 0080052700527 (toll free) In-country Tel: +45 44 54 01 01 Email: support.dk@leicabiosystems.com Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 4 Tel: 0080052700527 (toll free) In-country Tel: +44 1908 577 650 Email: support.uk@leicabiosystems.com USA: Tel: +1 (844) 534- 2262 (toll free) Direct International Tel: +1 760 539 1150 Email: TechServices@leicabiosystems.com Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 5: Table Of Contents

    Basic eSlide Viewing ........................20 View an eSlide ...........................21 View and Annotate eSlides.................... 23 Annotate eSlides ..........................23 Draw Annotations ........................23 Delete an Annotation ........................25 View eSlides With Annotations ....................25 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 6 Set Up the SpaceMouse Pro ......................42 Rotate the eSlide with the SpaceMouse Pro Device ................43 Navigate a Z-Stack Image with the SpaceMouse Pro Device ..............43 Index ............................. 44 Symbols..........................49 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 7: Introduction

    For primary diagnosis procedures, Aperio ImageScope DX supports only SVS files created by the Aperio AT2 DX scanner. Aperio ImageScope DX enables you to open TIFF files for non-diagnostic purposes only. The pan and zoom features are not available when viewing a TIFF file.

  • Page 8: Aperio At2 Dx Viewing Stations And Monitors

    “Appendix A: Perform a Monitor Quality Check” on page 38 for complete instructions. Aperio AT2 DX System Information This Aperio ImageScope DX User’s Guide contains details on viewing Slides created by the Aperio AT2 DX using the Aperio ImageScope DX viewing software. For further information, see: Aperio AT2 DX System Overview for information on the entire Aperio AT2 DX System.

  • Page 9: Aperio Imagescope Dx Overview And Quick Reference

    4: View and Annotate eSlides" on page 23. Open a Case in Aperio ImageScope DX You open a case in Aperio ImageScope DX from your organization’s medical device data system (MDDS). For information and instructions on using your MDDS, contact your site administrator.

  • Page 10: Aperio Imagescope Dx Quick Reference

    TIFF file. See "Save a Snapshot of the eSlide" on page 29. Synchronize navigation of all open eSlides. See "Synchronize Synchronize Navigation of Multiple eSlides" on page 31. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 11 Previous Go to the previous annotation. annotation Next annotation Go to the next annotation. See "Annotate eSlides" on page 23 for more information on using annotations. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 12 Status Bar Contains details about the current display. Aperio This symbol indicates that you are working in the Aperio ImageScope DX viewer. ImageScope DX indicator Rotation Dial Rotates the eSlide from 0 to 360°. See "Rotate the eSlide" on page 27.

  • Page 13: The Slide Tray

    Your eSlides will not display unless the monitor and viewing station are configured correctly. A red X also appears if you attempt to use Aperio ImageScope DX with a monitor other than the monitor approved for the Aperio AT2 DX System.
  • Page 14 To flag an eSlide for follow-up, right-click an eSlide and select Flag. A small red flag appears in the Slide Tray: To remove the flag, right-click the eSlide in the Slide Tray and select Flag. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 15: View Different Areas Of The Eslide

    Click inside the main Thumbnail to move to the corresponding area on the eSlide. Ì You can also track your navigation in the Thumbnail. See "Track Pan and Zoom Navigation With the Track Map" on page 29. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 16: Change The Viewing Zoom Level

    When you pan, rotate, or otherwise change the view of the eSlide, the "Rendering" message may appear. This means ImageScope DX is still adjusting the eSlide image, and you should wait until the message disappears before reviewing the eSlide for diagnostic purposes. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 17: Keyboard Shortcuts

    Activates the Counter annotation tool. Tool Annotations > Activates the Observation pen annotation tool. Observation Pin View > Full Screen None Expands ImageScope DX to a full-screen view. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 18: Close Aperio Imagescope Dx

    Close Aperio ImageScope DX To close Aperio ImageScope DX, go to the File menu, and select Exit. You can also click in the upper-right corner of the Aperio ImageScope DX window.

  • Page 19: Basic Eslide Viewing

    Open Case eSlides in Aperio ImageScope DX You can open one case at a time in Aperio ImageScope DX. When you open a case from your MDDS, Aperio ImageScope DX automatically closes any previously opened case before it opens your next case.

  • Page 20: Close A Case In Aperio Imagescope Dx

    Basic eSlide Viewing Use the Aperio ImageScope DX navigation tools to move around the eSlide and to view areas of the eSlide at different zoom levels. You do not need to focus the eSlide. Optimum focus is determined when the slide is scanned.

  • Page 21: View An Eslide

    View an eSlide To view an eSlide using the method described previously, follow these steps: With the eSlide open in Aperio ImageScope DX, set the zoom to 4X by clicking 4x on the Zoom Slider. Click the Pan and Zoom tool Drag the mouse pointer in any direction to pan around the eSlide.
  • Page 22 Click 4X to zoom out again and continue panning. See “Chapter 4: View and Annotate eSlides” on page 23 for information on advanced viewing techniques and adding annotations. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 23: View And Annotate Eslides

    View and Annotate eSlides This chapter provides information on viewing and annotating eSlides using Aperio ImageScope DX. Annotate eSlides Use annotations to mark an area of interest on an eSlide or to measure an object. You use the Annotation toolbar to select the annotation tool you want to use. To show the Annotation toolbar, go to the View menu, select Toolbars, and select Annotation.
  • Page 24 Counters are numbered automatically in the order they are placed. Add an observation pin Click the Observation Pin tool Click to mark areas of interest on the eSlide. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 25: Delete An Annotation

    Select the annotation you want to copy, and click the Copy tool Click the Paste tool . The duplicate annotation moves with your mouse pointer. Click to place the annotation in the eSlide. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 26: Move From One Annotation To The Next

    Viewer window. Draw Annotations With a Fixed Size or Fixed Aspect Ratio You can set a fixed size for annotations in the Aperio ImageScope DX options. The instructions below describe how to draw annotations using the fixed size. Draw an Annotation with a Fixed-Size Press the Ctrl key while drawing the annotation.

  • Page 27: Change Eslide Views

    To rotate the eSlide from 0° to 360°, drag the arrow around the Rotation Dial. The number in the center indicates the current rotation. To rotate the eSlide in 1 degree increments, click the left and right arrows at the bottom of the dial. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 28: Annotate A Rotated Eslide

    The annotation on the left was drawn after the eSlide was rotated. Rotate the eSlide Label Click the eSlide label to rotate it 90°. The system saves the eSlide label rotation when you save the eSlide. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 29: Save A Snapshot Of The Eslide

    In the Save Snapshot window, specify the file location and name, and then click Save to save the image. You can open the saved TIFF file in Aperio ImageScope DX; however, note that the pan and zoom features are not available when viewing TIFF files.

  • Page 30: Open Multiple Eslides

    You can open up to four eSlides at a time. Follow these steps to open and view multiple eSlides in the Aperio ImageScope DX Viewer window: Click the circle next to each eSlide you want to open. Selected images appear in the Slide Tray with push pin in the circle, as shown below.

  • Page 31: Synchronize Navigation Of Multiple Eslides

    Viewing and Navigating a Z-Stack Image Aperio ImageScope DX automatically opens a z-stack image to the best focused layer, as determined by your scanner when the slide is scanned. The number of layers and the layer separation (depth, in microns (µm), between the layers) is set during scanning.

  • Page 32: Viewing Different Z-Stack Layers

    Drag the Focus slider up and down to view different layers of the z-stack image. The number at the bottom of the Ì slider represents the current focus point. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 33: Drawing And Viewing Annotations On A Z-Stack Eslide

    To draw annotations on a z-stack eSlide: Drag the Focus slider to the desired z-stack layer. From the Annotation toolbar, select the annotation tool you want to use. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 34: Viewing Annotations On Different Z-Stack Layers

    You must use the Next and Previous buttons if you want to view the annotations on their z-stack layer. If you select the annotations manually by clicking them, the z-stack layer view is not adjusted. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 35: Aperio Imagescope Dx Options

    View the Aperio ImageScope DX Options To view or set Aperio ImageScope DX options, go to the Tools menu and select the option you want to view: Navigation, Annotations, Viewer, Tracking, or Monitor Quality Check.

  • Page 36: Navigation Options

    Click the Viewer tab to view or edit the Display Hover Popups for Images option. This option is enabled by default. If this option is enabled, you can point to an eSlide in the Slide Tray to view an enlarged version of the eSlide. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 37: Tracking Options

    You should check this test image once a month. See “Appendix A: Perform a Monitor Quality Check” on page 38 for complete instructions. Leica Biosystems Technical Services also uses this image for troubleshooting and maintenance purposes. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 38: Perform A Monitor Quality Check

    Go to the Tools menu, and select Monitor Quality Check. The Monitor Quality Check tab opens. Click Open Monitor Quality Check Image. The test pattern image appears in your Aperio ImageScope DX window. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 39 Following the direction of the yellow arrows in the example (from white to Ì outlined in Yellow. black), the change in intensity from one square patch to the next increases by the same relative amount. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 40 (30 - 60 cm). If you notice an issue with any of the test image evaluations, contact Leica Biosystems Technical Services. To close the test pattern image, go to the File menu and select Close Case.

  • Page 41: 3-D Mouse Quick Reference

    Aperio AT2 DX System. This section shows how the buttons on the optional 3Dconnexion SpaceMouse Pro map to Aperio ImageScope DX navigation tools. There is some setup required to use the SpaceMouse Pro device with ImageScope DX. See“Set Up the SpaceMouse Pro”...

  • Page 42: Set Up The Spacemouse Pro

    In the SpaceMouse Pro properties window, click Buttons. To map buttons 1 through 4: on the action box for button 1, select 3DConnexion, and select Application Use. Click Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 43: Rotate The Eslide With The Spacemouse Pro Device

    To view different layers of a z-stack image: Press and hold the ALT key on the SpaceMouse Pro device or your keyboard. Turn the controller cap to view the different z-layers. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 44: Index

    31 metadata 16 navigate 16 open multiple 14 camera tool.  Snapshot tool pan and zoom 16 view 13 case view eSlides 13 eSlide Label 16 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 45 9, 12 multiple eSlides 14 overview 9 overview Image toolbar 9, 10.  Image tools See also Aperio ImageScope window 9 Image tools eSlide information, view 10 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 46 Image 10 scan magnification 16, 22 View 12 Scanning Technician 20 track map clear 29 scan slides.  Aperio AT2 DX User’s Guide use 29 screen, expand 12 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 47 22 Zoom Slider description 12 example 9, 16 zoom in and out 16 z-stack images best focused layer 31 navigate with SpaceMouse Pro 43 viewing 31 Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 48 Index Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...

  • Page 49: Symbols

    The UV lamp within an equilateral triangle is intended to alert you to the presence of UV light within the product’s enclosure that may be of sufficient magnitude to constitute a risk to the operator. Aperio ImageScope DX User’s Guide, Revision A © Leica Biosystems Imaging, Inc. 2019...
  • Page 50 www.LeicaBiosystems.com/Aperio...

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