GE PD MiniTrap G-25 Product Booklet
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GE PD MiniTrap G-25 Product Booklet

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PD MiniTrap G-25
Product Booklet
Code:
28922529
PD MiniTrap G-25 contains
• 50 prepacked disposable PD MiniTrap™ columns containing
2.1 mL of Sephadex™ G-25 resin
• 4 adapters
• Instructions for use
Purpose
PD MiniTrap G-25 columns are prepacked and designed for
rapid, convenient sample clean-up of proteins and other large
biomolecules (>5000 M
).
r
PD MiniTrap G-25 columns can be used in a wide range of
applications such as desalting, buffer exchange and removal of
low-molecular weight compounds

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Summary of Contents for GE PD MiniTrap G-25

  • Page 1 PD MiniTrap G-25 columns are prepacked and designed for rapid, convenient sample clean-up of proteins and other large biomolecules (>5000 M PD MiniTrap G-25 columns can be used in a wide range of applications such as desalting, buffer exchange and removal of low-molecular weight compounds...

  • Page 2: Table Of Contents

    PD MiniTrap G-25 columns are used for desalting, buffer exchange and sample clean up. Small molecules like salt, free labels and other impurities are efficiently separated from the high molecular weight substances of interest.
  • Page 3 Group separation can be made using two different protocols, gravity protocol and spin protocol. GE provides an assortment of sample clean-up products. The different formats available are summarized in Table 1. Table 1. Product overview...

  • Page 4: Advice On Handling

    2. Advice on handling Protocol selection The separation can be made using two different protocols, gravity protocol or spin protocol, see Table 2 for protocol overview. Gravity protocol The liquid passes through the column by gravity force. • There is a slightly higher recovery and desalting capacity using gravity protocol compared to when using the spin protocol.
  • Page 5 Equilibration • It is critical to equilibrate the column since UV absorbing stabilizers are used in column packing. • Equilibration is most conveniently made by gravity also when using the spin protocol. Sample Application • The MiniTrap column is intended for sample volumes up to 0.5 mL. •...

  • Page 6: Safety Precautions

    Use gloves when discarding the storage solution. 4 Column assembly For use in the spin protocol the PD MiniTrap G-25 column should be assembled with adapter and collection tube as shown in Fig 1. Note: This column assembly may also be used for convenient handling of columns when using the gravity protocol.

  • Page 7: Gravity Protocol

    5 Gravity protocol 1 PD MiniTrap G-25 preparation • Remove the top cap and pour off the column storage solution. • Remove the bottom cap. 2 Column equilibration • Fill up the column with equilibration buffer and allow the equilibration buffer to enter the packed bed completely.

  • Page 8: Spin Protocol

    • Remove the top filter using forceps. • Remove the bottom cap. • Put the PD MiniTrap G-25 into a 15 mL collection tube by using the column adapter, see Fig 1 2 Column equilibration • Fill up the column with equilibration buffer and al- low the equilibration buffer to enter the packed bed completely.

  • Page 9: Recovery And Desalting Capacity

    Fig 2." 1000 Protein conc. (µg/mL) NaCl in eluate (mM) Elution volume (mL) Fig 2. Removal of NaCl from albumin solution with PD MiniTrap G-25. The albumin is eluted in volume fractions between 0.5 to 1.5 mL (indicated by arrows).

  • Page 10: Column Characteristics

    8 Column characteristics Matrix Sephadex G-25 resin Particle size range 85 to 260 µm Packed bed dimensions 0.97 × 2.8 cm (2.1 mL) Maximum sample volume 0.5 mL Volume of eluted sample gravity 1.0 mL Volume of eluted sample spin 0.5 mL Desalting Capacity >90%...

  • Page 11: Ordering Information

    9 Ordering information Product Pack size Code PD MiniTrap™ G-25 28-9180-07 Related products Pack size Code No. PD-10 Desalting 17-0851-01 Columns PD SpinTrap G-25 28-9180-04 PD MultiTrap G-25 4 × 96-well filter plates 28-9180-06 PD MidiTrap G-25 28-9180-08 PD MiniTrap G-10 28-9180-10 PD MidiTrap G-10 28-9180-11...
  • Page 12 Buckinghamshire, HP7 9NA GE monogram, SpinTrap, MultiTrap, GE Healthcare Bio-Sciences Corp., MiniTrap, MidiTrap, HiTrap, HiPrep, 100 Results Way and Sephadex are trademarks of GE Marlborough Healthcare companies. MA 01752 Kathon is a trademark of Dow Chemical Co., MilliQ is a trademark of Merck KGaA.

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