Low-Pressure Gradient System - Hitachi CHROMASTER 5160 Instruction Manual

1.2 Low-Pressure Gradient System

(1) A mobile phase is fed by the pump through the degassing unit.
(2) A sample is injected from the autosampler.
(3) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(4) Each component separated from the sample is then
detected as a signal by the detector.
In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of a mobile
phase is made to vary with time in chromatographic separation.
Injection valve
Syringe valve
Degassing unit
Fig. 1-2 Low Gradient System
Wash fluid
Mobile phase
Drain
Low gradient unit proportioning valve
1 - 2
Chromaster
organizer
Chromaster
5410/5420/5430 detector
Chromaster 5310
column oven
Chromaster 5210/5260
autosampler
Wash pump
Chromaster 5110/5160
pump