Hitachi L-2100 Instruction Manual
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Hitachi L-2100 Instruction Manual

Hitachi L-2100 Instruction Manual

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INSTRUCTION MANUAL
FOR
MODEL L-2100 PUMP
Copyright
Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan.
Part No. 890-9101

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Summary of Contents for Hitachi L-2100

  • Page 1 INSTRUCTION MANUAL MODEL L-2100 PUMP  Copyright Hitachi High-Technologies Corporation 2002. All rights reserved. Printed in Japan. Part No. 890-9101...
  • Page 2 Thank you very much for purchasing Hitachi Model L-2100/2130 pump, which has been specifically designed for automatic analysis in a liquid chromatograph system. The Model L-2100/2130 pump is intended for use by persons having a basic knowledge of chemical analysis. Remember that improper use of analytical instruments, chemicals or samples would result not only in wrong analytical data but also in consequences adverse to safety.
  • Page 3 - 2 -...
  • Page 4 IMPORTANT Precautions on Electromagnetic Wave Interference Possible Electromagnetic Wave Interference Caused by This Instrument Where this instrument is used in a residential area or an adjacent area thereto, it may cause interference to radio and television reception. To prevent this, use the specified system connection cables in strict accordance with the instruction manual.
  • Page 5 • Check that any other device connected with the instrument is not affected by electromagnetic interference. Warranty on Product The Model L-2100 pump is warranted to be free from defects in material or workmanship under normal use within the product specifications indicated in this manual and under conditions given below.
  • Page 6 Failure due to improper handling or maintenance by the user. (g) Failure due to maintenance or repair by a service agent not approved or authorized by Hitachi High- Technologies Corporation. (h) After disposal of this instrument, or after its resale without Hitachi High-Technologies Corporation approval.
  • Page 7 Before installation of the instrument, the customer is requested to prepare necessary utilities referring to this manual. When relocation of the instrument becomes necessary after initial installation (delivery), please notify your local Hitachi High- Technologies sales representative or service office. - 6 -...
  • Page 8 For the customers to acquire in-depth understanding of the analytical instruments, technical seminars and customer training courses are available at Hitachi High-Technologies Corporation or your site. For further information, contact your local Hitachi High-Technologies Corporation sales representative. (The technical seminars and customer training courses are available on a chargeable basis.)
  • Page 9 The isocratic system has a simple configuration in which single- solvent analysis is performed. Washing solution Eluent System power supply unit Model L-2400 UV detector Model L-2300 column oven Column Model L-2200 autosampler Model L-2100/2130 pump Degasser Drain 1 - 1...
  • Page 10 Isocratic System An eluent is fed by the pump through the degasser. A sample is injected from the autosampler. The injected sample is separated through the column which is maintained at a constant temperature by the column oven. Each component separated from the sample is then detected as a signal by the detector.
  • Page 11 Model L-2300 column oven Column Model L-2200 autosampler Low-pressure gradient unit Mixer Model L-2100/2130 pump Degasser Drain Each of plural eluents is fed from the degasser to the low- pressure unit. In the low-pressure unit, the eluents are mixed at a pre- specified ratio.
  • Page 12 Washing Model L-2400 solution UV detector System power Model L-2300 supply unit column oven Column Model L-2100/ 2130 pump Mixer Model L-2200 Model L-2100/ autosampler 2130 pump Degasser Drain From the degasser, each of plural eluents is applied to the pump.
  • Page 13 NOTICE: Installation of this instrument shall be carried out by or under supervision of qualified service personnel of Hitachi High-Technologies Corporation or its authorized service agent. Before installation of this instrument, the customer shall make preparations for satisfying the installation requirements in accordance with this manual.
  • Page 14 Installation Place 2.2 Installation Place When selecting an installation place for this instrument, be sure to check the following conditions: Operating Ambient Temperature 4 to 35 °C Temperature variations during measurement should be minimized. Take sufficient care to prevent dew condensation.
  • Page 15 Free from an amount of dust and dirt. Contamination with dust and dirt could deteriorate the performance of the instrument. (g) No abrupt fluctuations in power voltage. Fluctuations in power voltage could cause noise in analytical data. (h) Do not connect the instrument to a power line used by an electric device that is not equipped with a noise suppressor circuit (e.g., stirrer, vibrator).
  • Page 16 Items to be Prepared by the User 2.3 Items to be Prepared by the User Power voltage : 100 to 240 V AC 2.3.1 Power Supply Fluctuation ..Within ±10% with respect to rated voltage Frequency : 50 or 60 Hz Fluctuation ..Within ±0.5 Hz with respect to rated frequency Power...
  • Page 17 When relocation of the instrument becomes necessary after initial installation (delivery), please notify your local Hitachi High-Technologies sales representative or service office. 2.4 Checking the Contents After unpacking, check the contents of each case against the packing list attached to it.
  • Page 18 Assembly 2.5 Assembling The L-2000 Series system is provided with a system power 2.5.1 Power Supply supply unit which is capable of connecting two pumps, one Unit autosampler, and one detector. The column oven has its own power cord. In an instance where provision of a single system power supply unit is insufficient for the use of two detectors, use an AC adapter suitable for each instrument (50 W AC adapter for the pump/autosampler, 100 W AC adapter for the detector).
  • Page 19 Isocratic System/Low-Pressure Gradient System Connect the power cables of the system power supply unit and column oven to AC power source. For the L-2100/ 2130 pump and L-2100 pump, connect the 50 W cable from the system power supply unit to the instrument power connector.
  • Page 20 High-Pressure Gradient System Connect the power cables of the system power supply unit and column oven to AC power source. For the L-2100/ 2130 pump and L-2100 pump, connect the 50 W cable from the system power supply unit to the instrument power connector.
  • Page 21 2.5.3 Isocratic System/Low-Pressure Gradient System System power supply unit Model L-2400 UV detector e-Line cable Model L-2300 column oven e-Line cable Model L-2100 pump e-Line cable Model L-2100/2130 pump High-Pressure Gradient System e-Line cable Model L-2400 UV detector System power...
  • Page 22 Assembly In the L-2000 Series, provide inlet tubing to the pump and waste 2.5.4 Piping solution tubing through the right piping section of the drain Arrangement reservoir. Provide tubing from the pump to the autosampler, column and detector through the center piping section of the drain reservoir.
  • Page 23 Washing solution Eluent Inlet tube Detector cell Model L-2400 UV detector Model L-2300 column oven Column Injection valve Model L-2100 pump Syringe valve Model L-2100/2130 pump Degasser Drain Drain valve 2 - 11...
  • Page 24 Eluent Washing solution Inlet tube system power supply unit Detector cell Model L-2400 UV detector Model L-2300 column oven Column Injection valve Model L-2100 pump Syringe valve Drain valve L-2100/2130 pump Degasser Mixer Drain Proportioning valve Four-way joint 2 - 12...
  • Page 25 Model L-2300 column oven unit Injection valve Syringe valve Model L-2100/ 2130 pump Model L-2100 pump Drain Drain valve Where the chromato data station is not used for controlling 2.5.5 Module Unit operations, each module can be operated using the optional UI Operating pad.
  • Page 26 Assembly Using the UI Pad (a) Functions of common control keys LOCK OFF INSERT SET UP UTLTY PROG Key Designation Function UTLTY Starts instrument parameter setting. Starts GLP function setting. Available for displaying a logbook of component parts of each module, triggering a reset, and locking the UI pad keys. PROG Starts program editing.
  • Page 27 2.5.5 (b) Contents on screen The parameter input window and the program setup window are described below. 1) Input of a single numeric value: Parameter name Input range PROGRAM No. (1 - 9) Input part 2) Input of plural numeric values Parameter name Input item LAMP CHANGE (MM, DD, YYYY)
  • Page 28 Assembly (c) Numeric Value Input Procedure 1) The cursor is indicated at the top of the input part. The previous numeric value is presented in the input part. 2) Numeric and decimal point key inputs are accepted in each allowable range. 3) To define an input numeric value, press the ENT key.
  • Page 29 2.5.5 Mounting the UI Pad (a) Remove the front panel from the instrument. Front panel (b) Remove the metal lid from the instrument. Meal cover 2 - 17...
  • Page 30 Assembly (c) Run the flexible tube of the UI pad through the metal lid. Metal cover Flexible tube UI pad (d) Turn up the lid of the connector, and insert the flexible tube of the UI pad as far as it will go. Flexible tube Connector UI pad...
  • Page 31 2.5.5 (e) Turn down the connector lid, and mount the UI pad. Flexible tube Connector UI pad Attach the metal lid dedicated for the UI pad. Flexible tube Metal cover UI pad 2 - 19...
  • Page 32 Assembly (g) Set the of the UI pad to the of the instrument. UI pad (h) Mount the front panel dedicated for the UI pad on the instrument. Front panel 2 - 20...
  • Page 33 2.5.6 The wiring cover is provided on the rear of each instrument. 2.5.6 Instrument-to- Instrument-to-instrument fastening can be made by shifting each Instrument wiring cover as shown below. Fastening Remove the retaining screws, and detach the wiring cover. Wiring cover Retaining screw Shift the mounting position of each wiring cover.
  • Page 34 FUNCTION 3.1 The Instrument Keypad 3.1.1 Name and Function of Each Part Main switch Right door Drain Drain Valve Left door Leak sensor Fig. 3-1 Front View • Main switch : Turns on/off power supply. • Doors : Two doors are provided. •...
  • Page 35 3.1.1 I/F board mounting space Wiring covers Ground terminals Wiring covers Pump can start by an Pump can stop by an Pressure value is output external signal. external signal. to the exterior (Approx. 49 MPa)/1 V. Fig. 3-2 Rear View Wiring covers : Upon detaching the left-side cover (as viewed from the front), the e-Line...
  • Page 36 3.10 Table 3-1 Name and Function of Each Terminal at Rear Panel Classi- Contents of Contents of Name Function Remarks fication Signal Processing • Timer • TIMER No. and Event code Output TIMER Contact The ON/OFF (1 to 4) turns signal can on/off output to...
  • Page 37 3.1.2 Part Name and Function of UI Operation Keys Pad (option) Each key on the UI pad (option) type operation panel is explained here. STOP MANUAL LOCK OFF START INSERT INITIAL PUMP PURGE SET UP UTLTY PROG PUMP Starts/stops operation of the pump. Pressing this key ON/OFF starts the pump.
  • Page 38 Used for erasure of data under input. Also usable for erasure of an error message at occurrence of an error. Used for deleting a step in a sequential program. Inputs a numeral. NOTE: If a mistake is made during operation, press the ESC key.
  • Page 39 [IN] Waiting for execution of analysis. A liquid is being fed. [--] Waiting for execution of analysis. The pump is being stopped. [MN] The MANUAL mode is selected. [PG] The purging operation is under execution. [LK] The keyboard of the operation panel is locked. (b) 2nd monitor screen P-UNIT P.MAX P.MIN...
  • Page 40 (c) Changeover of monitor screen The monitor screen transition is as follows. If the link program function is OFF, the 4 monitor screen will not appear. 1) 1st MONITOR screen TIME %A FLOW PRESS [--] 0.000 2) 2nd MONITOR screen ESCAPE P-UNIT P.MAX P.MIN...
  • Page 41 Operation Status Fig. 3-3 is a flow chart that shows the transition of operational status. ( indicates depression of a key.) <Turning on power> (does not function unless the pump is on) START/STOP INITIAL START/STOP MANUAL MANUAL INIT MANUAL INITIAL : Programming mode status in which system waits for start.
  • Page 42 Specifications (1) L-2100 (a) Liquid feed system: Dual plunger type reciprocating pump( Tandem Type) (b) Maximum operating pressure: 39.2 MPa (c) Flow rate range 0.001 to 2.499 mL/min (d) Pressure limit range 0 - 39.2 MPa (e) Delivery volume of No.1 plunger stroke...
  • Page 43 Possible Ambient temperature 4 to 35°C (condensation unallowable) Humidity: 45 to 85% RH Power supply: 24V DC, 21.A Dimensions: 260(W) × 500(D) × 235(H) mm Weight: About 15 kg (2) L-2130 (a) Liquid feed system: Dual plunger type reciprocating pump( Tandem Type) (b) Maximum operating pressure: 39.2 MPa (c) Flow rate range...
  • Page 44 (l) Pump control mode: Single-Liquid delivery mode Low pressure gradient mode High pressure gradient mode (m) Contact input/output terminals: Event timer; 4 channels (n) Communication: e-Line OUTPUT: BUSY INPUT: GRADIENT START IN ERROR IN (o)Pressure zero adjustment: Possible Ambient temperature 4 to 35°C (condensation unallowable) Humidity: 45 to 85% RH...
  • Page 45 OPERATION The method of pump operation using the optional UI pad is described here. Preparatory Operations Explained here is the operating method for using the L-2130 pump. 4.1.1 Setting of Pump Mode This section shows how to set the pump in the single-liquid delivery mode (FLOW mode).
  • Page 46 GR MODE LOW HIGH V. HIGH FLOW 4 [4][ENTER] LOW: Low pressure gradient mode HIGH: High pressure gradient mode V.HIGH: High pressure gradient with solenoid valves mode FLOW: Isocratic mode SETUP PUMP MODE CONTRAST S.NO. FLOW 02AB-111 <POWER OFF> Turn the power off once. <POWER ON>...
  • Page 47 4.1.2 Programming Operation This section discusses the programming of the FLOW mode. An example is given where upper pressure limit is 20.0 MPa, lower pressure limit is 0 MPa, analysis time is 0 min, flow rate is 1.0 mL/ min. PROGRAM No.
  • Page 48 PRESSURE: [--] 20.0 Input the upper and lower limit pressure values. TIME FLOW EVENT [--] 1.000 Input time, flow rate and event. Up to 4 entries can be made per step for event. Input an event to be executed within the entered time. TIME FLOW EVENT [--]...
  • Page 49 4.1.3 Manual Operation This is used for supplying liquid under constant conditions without using a program. (1)Constant Flow Rate Mode This section explains the setting of liquid delivery system at constant flow rate. An example is given where flow rate at 1.0 mL/min, upper pressure limit at 15.0 MPa and lower pressure limit at 0 MPa.
  • Page 50 (2)Constant Pressure Mode This section explains the setting of liquid delivery system at constant pressure. An example is given where initial flow rate is 1.0 mL/min, at a pressure of 15.0 MPa. Monitor screen MANUAL MANUAL: FLOW=1, PRESS=2 [MN] Select the constant pressure mode. CONSTANT PRESS: INITIAL FLOW PRESS [MN]...
  • Page 51 Measuring Operation WARNING Ignition of Flammable Chemicals! • Beware of ignition hazard when using flammable chemicals such as organic solvents. • Always check the following conditions. If an abnormality is found, stop operation immediately. • Leakage of solvent or waste solution •...
  • Page 52 4.2.1 Basics of Operation When the settings are all finished, carry out operation in the following procedure. Make sure the pump, column, detector and data processor are properly connected. Set the mobile phase and waste solution bottle in place. Start the pump and wait until the flow path is filled with the mobile phase.
  • Page 53 4.2.2 Analyzing Operation SET PROGRAM allows the generation of a maximum of 9 programs. You can execute any of the programs selected or generated via the SET PROGRAM. For a gradient analysis, implement the following procedure. Select [INITIAL] , and select or generate a program for eluent. Turn on each pump.
  • Page 54 In case that an event setting is desired independent of FLOW , only the event code showed be entered. Press ENTER to skip all the entries you do not want until you reach event column. In the SET PROGRAM mode, the interpolation of mixing ratio, flow rate, etc. that occurs in a time program is as follows.
  • Page 55 (1) New Generation of a Program This section shows the generation of a new program. SET PROG (SET PROG No.) No. of program to be ENTER generated (m) (MODIFY=0, NEW=1) ENTER (MAX) Upper limit pressure ENTER (MIN) Lower limit pressure ENTER level level...
  • Page 56 If pump pressure falls below the level set here, the pump automatically stops. The set time can be from 0.0 to 600.0. Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are from 0.00 to 9.999 (L-2130).
  • Page 57 2) Deletion of Program How to delete a program is shown next. SET PROG (SET PROG No.) No. of program to be deleted (m) ENTER (MODIFY=0, NEW=1) ENTER ESCAPE (3) Deletion of Step How to delete one step in a program is shown next. SET PROG (SET PROG No.) No.
  • Page 58 Program number can be from 1 to 9. The contents of the existing program will be deleted. The monitor screen reappears. Program number can be from 1 to 9. For editing, input 0. For generating a new program, input 1. If pump pressure rises beyond the level set here, the pump automatically stops.
  • Page 59 (4) Addition of Step SET PROG (SET PROG No.) No. of program for adding ENTER a step (m) (MODIFY=0, NEW=1) ENTER (MAX) Upper limit pressure ENTER (MIN) Lower limit pressure ENTER level level (TIME) Time point of step added (Ti) ENTER (FLOW) Set flow rate (Fi)
  • Page 60 If pump pressure falls below the level set here, the pump automatically stops. The Input the time point of the step you want to add. Flow rate valid entries are from 0.00 to 2.499 (L-2100). Flow rate valid entries are from 0.00 to 9.999 (L-2130).
  • Page 61 (5) Correction of Step Step correction method is explained here. SET PROG (SET PROG No.) No. of program containing step to be ENTER deleted (m) (MODIFY=0, NEW=1) ENTER (MAX) Upper limit pressure ENTER (MIN) Lower limit pressure ENTER level level (TIME) Time point of step added (Ti) ENTER...
  • Page 62 0.00 to 2.499. When a correction is unnecessary, press the ENTER key. Valid flow is from 0.00 to 2.499(L-2100). Valid flow is from 0.00 to 9.999(L-2130). Enter the event code. When a correction is unnecessary, press the ENTER key.
  • Page 63 (6) Deletion of Parameter The parameter deletion method is shown next. SET PROG (SET PROG No.) No. of program containing parameter to ENTER be deleted (m) (MODIFY=0, NEW=1) ENTER (MAX) Upper limit pressure ENTER (MIN) Lower limit pressure ENTER level level ENTER DELETE...
  • Page 64 Program number can be from 1 to 9. For correction, input 0. For generating program, input 1. If pump pressure rises beyond the level set here, the pump automatically stops. If pump pressure falls below the level set here, the pump automatically stops. Press the key until the step to be corrected appears on the screen.
  • Page 65 Setting of Utility Functions Utility functions such as zero adjustment of pump pressure, selection of pump pressure unit and column flushing are settable as follows. 4.3.1 Pressure Zero Adjustment You do not need to perform a pressure zero adjustment every day. The zero point may change due to a secular change of the pressure sensor, etc.
  • Page 66 STANDARD Monitor screen MOTOR CONTROL is set to STANDARD. FIX STEP AREA <0, 30-300> Enter a FIX STEP AREA. Input range is 0, and from 30 to 300. Monitor screen NOTICE: Select “STANDARD” for Model L-2100/2130 pump. 4 - 22...
  • Page 67 4.3.3 Purge Flow Rate Set a flow rate when executing purge. Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK= OTHERS=6 Select CONDITION. CONDITION SET Z-ADJ=1 MOTOR-CTRL=2 PURGE=3, CALIBRATION=4 Select PURGE. PURGE FLOW: FLOW [--] <0.001-2.499> 2.499 Enter a purge flow rate. Monitor screen Pressing the Purge key in PUMP ON status activates purging at the set flow rate.
  • Page 68 4.3.4 Flow Calibration Set a parameter of flow rate calibration. Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK= OTHERS=6 Select CONDITION. CONDITION SET Z-ADJ=1 MOTOR-CTRL=2 PURGE=3, CALIBRATION=4 Select CALIBRATION. CALIBRATION FLOW=1 PRESS=2 Select FLOW. FLOW CALIB. 0.97 – 1.03 1.000 Enter a parameter o flow rate calibration Monitor screen...
  • Page 69 4.3.5 Pressure Calibration Set a parameter of pressure calibration. Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK=5 OTHERS=6 Select CONDITION. CONDITION SET Z-ADJ=1 MOTOR-CTRL=2 PURGE=3, CALIBRATION=4 Select CALIBRATION. CALIBRATION FLOW=1 PRESS=2 Select PRESS. FLOW CALIB. 0.900 – 1.100 1.000 Enter a parameter of pressure calibration Monitor screen...
  • Page 70 4.3.6 Pressure Unit Pump pressure unit is selectable from bar, psi, kgf/cm and MPa. Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK= OTHERS=6 Select UNIT. PRESS UNIT: <bar=1, psi=2, kgf/cm2=3, [--] <1-4> MPa=4> Select pressure unit. Monitor screen Pressure indication unit is set to selected one.
  • Page 71 4.3.7 Column Flushing Automatic pump stop and/or column flushing after completion of analysis is settable. By setting for column flushing, the flushing will be done automatically after the end of analysis. When a salt solution has been used for analysis, for example, elution of the salt component can be prevented by flushing the column with water.
  • Page 72 FLUSH MODE: PARAM.=1, PROG.(NO.9)=2 [--] Enter “1” for column flushing under constant conditions, or “2” for column flushing using a gradient. [PARAM] SET DELAY TIME: TIME (min) [--] <1.0-600.0> 600.0 Input the time period from the end of analysis to the start of column flushing.
  • Page 73 Following is the relation between delay time and column flush. Delay time Column flush Gradient end With AUTO STOP at YES When YES is selected for COLUMN FLUSH ∗ Analysis 1 Analysis 2 Analysis 3 Analysis n Delay time COLUMN FLUSH Stabilizing time Pump stop ∗...
  • Page 74 4.3.8 Event Signal Code The event should be executed manually. The event function works upon input of an event code. It is used for starting an external device, for stopping the pump, for sounding the buzzer, etc. Also, if a pressure limiter error occurs, a signal can be output at event code No. 4. Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH=...
  • Page 75 Event codes and related operations are explained below. Control Item Event Code Remarks Timer 1st digit: Timer no. 2nd digit: Action pattern Start ∗ Contact closes for 1 sec. Action Contact point opens. Open Signal Contact turns closes. off. Signal Close Start marker ∗...
  • Page 76 4.3.9 Error In Signal Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK= OTHERS=6 Select OTHERS. OTHERS ERROR IN=1 LEAK SENSOR =2 Select ERROR IN. ERROR IN SIGNAL DISABLE=0 PUMP OFF=1 DISABLE: The pump is not used ERROR signal to stop the unit in e-Line PUMP OFF: The pump stops the flow when it received the ERROR signal in e-Line Monitor screen...
  • Page 77 4.3.10 Leak Sensor Monitor screen UTLTY UTILITY CONDITION=1 UNIT=2 FLUSH= EVENT 4 LINK= OTHERS=6 Select OTHERS. OTHERS ERROR IN=1 LEAK SENSOR =2 Select LEAK SENSOR. LEAK SENSOR OFF=0 ON=1 Select “ON” for using the leak sensor or “OFF” for avoiding its Monitor screen 4 - 33...
  • Page 78 Setting of GLP Functions Explained here are the confidence functions such as logbook display and resetteng. 4.4.1 Logbook Logbook can be displayed or reset. Logbook Display Logbook can be displayed in the following procedure. Monitor screen GLP: LOGBOOK=1, PRESS M.=2, [--] KEY LOCK=3 Select LOGBOOK...
  • Page 79 RESET LOGBOOK/RESET: [--] (MM, DD, YYYY) 2 25 2002 Logbook is reset. NOTICE: Input the date of pump seal replacement. Input is allowed in the order of month (M), day (D) and year (Y). Upon resetting, total flow value is reduced to 0 and the integration of flow starts.
  • Page 80 4.4.2 Pressure Monitor The number of double-speed section errors in each measurement can be indicated. A double-speed section error indicates that the pressure did not return to the reference level (previous pressure) even after double-speed control (an increase of pump motor speed) was effected to compensate for a pressure drop.
  • Page 81 3.8.3 4.4.3 Key Lock Keyboard keys can be locked. Monitor screen GLP: LOGBOOK=1, PRESSURE M.=2, [--] KEY LOCK=3 Select KEY LOCK. KEY LOCK: YES=1, NO=0 Locks the keys on the operation panel in order to prevent undesired key entries. Monitor screen (LOCK OFF) Key lock can be released by the [ ESCAPE (LOCK OFF) ] key.
  • Page 82 The following items are required for performance check. Prepared for Performance Component Units of Liquid Chromatography System Check • Pump (Hitachi L-2100/2130 or equivalent) • Detector (Hitachi L-2400 or equivalent) • Data processing unit • Waste liquid bottle If deaeration of detergent is inadequate, full NOTE: performance may not be provided.
  • Page 83 Detector (wavelength 250 nm, time constant 0.1 s) Waste liquid bottle Load resistance coil stainless pipe 0.1 mm i.d. × 10 m long(L-2100) stainless pipe 0.25 mm i.d. × 10 m long(L-2130) Autosampler (Injection valve) (Syringe valve) Pump...
  • Page 84 5.1.2 Prepare other units to be connected to the autosampler as described in the respective instruction manuals. Put the drain tube from the detector into the exclusive detector waste liquid bottle. If this tube is put into other waste liquid bottle such as for the Model L-2200 autosampler, baseline fluctuation may be caused due to unstable back pressure.
  • Page 85 (11) Set the pump flow rate to 0.2mL/min L-2100 . Set the pump flow rate to 1.0mL/min L-2130 . (12) Set the detector wavelength to 250 nm. Set the detector time constant to 0.1 s. (13) Set the following conditions in the data processing unit.
  • Page 86 × RSD of variation xi: Area value due to each injection, Number of injections Make sure the RSD value is 0.075% or less. However, this value represents the reproducibility of not the pump alone but the entire chromatographic system. Therefore, the value varies with each stability of the autosampler and detector, accuracy of the data processing unit and its processing parameters.
  • Page 87 5.2 Periodic Maintenance Perform period maintenance and inspection according to Table 5-1. This prevents problems and ensures that your system meets specifications. Table 5-1 Period Maintenance and Inspection Check Location Procedure Remarks Small particles Wash the solvent filter Intrusion of particles (dust) are often with an ultrasonic cleaner into the pump causes...
  • Page 88 Check Location Procedure Remarks The flow path Wash the filters with an The filters prevent filter of the drain ultrasonic cleaner every wornout fragments of the valve is easily 4 months. pump seal from flowing contaminated. into the column. If the filter is contaminated, the pressure increases abnormally.
  • Page 89 If the pump should become faulty, contact the dealer NOTICE: from whom you purchased the pump. Repair must be left to personnel who have undergone Hitachi High- Technologies Corporation technical training since potential hazards are involved. 5.4.1 Error Messages and Proper Measures...
  • Page 90 Operation Function Error message Power ON [INITIAL]ialization ROM ERROR Error RAM ERROR generating EPROM ERROR condition MOTOR [INITIAL]IALIZE ERROR E-LINE ERROR Power ON Pump operation PRESSURE MAX LIMIT ERROR Error PRESSURE MIN LIMIT ERROR generating EXTERNAL STOP! condition Fig. 5-1 Error Generation and Error Messages 5 - 9...
  • Page 91 LCD. Use the CL key to erase the error messages. Unless the cause of an error is eliminated, the same error message will display again. For units other than the Model L-2100/2130, check the corresponding instruction manuals. Table 5-2 Model L-2130 Error Messages...
  • Page 92 5.4.2 Troubleshooting Table 5-3 offers a guideline for the troubleshooting of the pump. Refer to this table first, if a problem occurs. Table 5-3 Model L-2100/2130 Troubleshooting Symptom Cause Measure Input mode cannot be The monitor screen is Set the programming mode...

  • Page 93: Parts Replacement

    655-1080 Seal for L- Pump head 640h 2130 885-2920 Seal Drain valve 1920h 890-1128 Plunger for Pump head — L-2100 890-1125 Plunger for Pump head — L-2130 890-1206 Check valve Pump head — (on discharge If check valve valve) for L-...
  • Page 94 810-1005 Check valve Pump head — Discharge valve Suction valve (on discharge If check valve Packing (B) valve) for L- doesn't work 810-1344 2130 properly, clean. If this doesn't 810-1004 Check valve Check valve 810-1325 solve the (on suction problem, replace valve) for L- Packing (A) the valve.
  • Page 95 810-1310 PCTFE push screw 3 mm OD PTFE tube (3.2 ID) 13.5 810-1311 PCTFE push screw 2 mm OD PTFE tube (2.2 ID) 810-1312 PCTFE push screw 2 mm OD PTFE tube No. 10 32 UNF (2.1 ID) 10.5 638-1099 SUS ferrule 1/16 inch OD SUS (SUS 316)
  • Page 96 (cont’d) Part No. Name Shape and Specifications Position Used 0.33 mm ID × 1.57 mm OD × • Outlet tube for F275144 PTFE tube 10 m UV detector • UV detector back pressure tube for high pressure gradient elution • Reaction coil for reaction LC 0.5 mm ID ×...
  • Page 97 (cont’d) Part No. Name Shape and Specifications Position Used 885-1601 Nipple (made of No. 10 Same as above SUS) 32 UNF 885-2179 Nipple (made of No. 10 No. 10 Same as above Daiflon) 32 UNF 32 UNF 21.5 6 - 5...

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