Hitachi CHROMASTER 5160 Manuals

Manuals and User Guides for Hitachi CHROMASTER 5160. We have 1 Hitachi CHROMASTER 5160 manual available for free PDF download: Instruction Manual

Hitachi CHROMASTER 5160 Instruction Manual

Hitachi CHROMASTER 5160 Instruction Manual (1022 pages)

Brand: Hitachi | Category: Water Pump | Size: 14.36 MB

Table of Contents

Section 1

20
- Preface
  3
- About this Manual
  4
- Important
  5
- Warranty on Product
  5
- Installation, Relocation and After-Sale Technical Service
  7
- Technical Seminars and Training Courses for Users
  8
- Estimated Life Time of the Instrument
  8
- Caution on Disposal of Instrument
  9
- Disposal of this Instrument and Its Parts
  9
- Precautions on CE Conformity Marking
  9
- Other Precautions
  10
- Information for Users on WEEE (Only for EU Countries)
  10
- Safety Summary
  12
- General Safety Precautions
  13
- WARNING Precautions in the Manual
  14
- CAUTION Precautions in the Manual
  16
- WARNING and CAUTION Labels on Instrument
  17
- NOTICE Precautions
  18
  - Table of Contents
    20
- Outline
  23
- Isocratic System
  23
- Low-Pressure Gradient System
  24
- Functions
  26
- Name and Function of each Part on Pump
  26
- Name and Function of each Part on UI Pad (Option)
  31
- Specifications
  35
- Operation
  38
- Basic Operation
  38
- Power Supply
  38
- Parameter Setting
  44
- Operational State
  46
- ON/OFF Setting
  47
- Purging Operation
  47
- Manual Operation
  48
- Programming Operation
  50
- Washing after Operation
  52
- Exit Operation
  52
- UTLTY Setting
  55
- Zero Pressure Calibration
  55
- Pump Motor Control Setting
  56
- Purging Flow Rate Setting
  57
- Flow Rate Calibration Setting
  58
- Pressure Calibration Setting
  59
- Pressure Unit Setting
  60
- Column Flush Setting
  61
- Event Code Setting
  64
- Link Program Function
  66
- Error out Signal Setting
  75
- Plunger Wash Pump Setting
  75
- Leak Sensor Setting
  76
- Glp
  77
- Pump Seal Logbook
  77
- Plunger Wash Pump Logbook
  78
- Auto Purge Valve Logbook
  79
- Dynamic Mixer Logbook
  80
- Low Gradient Unit Logbook
  81
- Pressure Monitor
  83
- Key Lock
  84
- Analysis Operation
  85
- Analyzing Operation
  87
- Operation Procedure
  87
- Setup
  98
- Pump Mode Setting
  98
- LCD Contrast Setting
  100
- Before Performing Analysis
  101
- Selection of Mobile Phase and Cautions on Handling
  103
- Characteristics of Organic Solvents
  106
- Cautions on Static Electricity
  111
- Preparation
  112
- Degassing and Removing Dust from Mobile Phase
  112
- Degassing Mobile Phase
  112
- Removing Dust from Mobile Phase
  115
- Sample Pretreatment
  116
- Maintenance & Inspection
  117
- Performance and Specifications Check
  117
- Performance Check Preparation
  117
- Preparing the System for Operation
  118
- Flow Rate Precision Check
  121
- Periodic Maintenance
  123
- Liquid Leak Sensor Check
  123
- Periodical Inspection
  125
Section 2

209
- General
  211
- Configuration of Liquid Chromatograph System
  213
- Outline of Chromaster Organizer
  213
- Isocratic System
  214
- Low-Pressure Gradient System
  215
- Functions
  217
- Name and Function of each Part on Chromaster Organizer
  217
- Specifications
  219
- Fuse Replacement
  220
- Replacement Parts
  220
- Replacement Procedure
  220
- Troubleshooting
  222
- Index
  249
Section 3

266
- Isocratic System
  269
- Overview
  269
- Explanation of Power Switch and Others on Front Panel
  273
- Functions
  273
- Part Names and Functions of UI Pad (Option)
  274
- Rear Panel
  276
- Principle of Operation
  277
- Specifications
  279
- Basic Operations
  283
- Operation
  283
- Turning on Power Supply
  283
- Parameter Input Method
  287
- Auto Zero
  290
- Setting of Measuring Wavelength
  290
- Shutdown
  291
- Washing after Use
  291
- Setting the Offset
  292
- Utility Setting
  292
- Setting the Time Constant
  294
- Setting the Use or Non-Use of Time Program
  295
- Setting the Polarity
  296
- Other Settings
  297
- GLP-Related Functions
  303
- Logbook of D2/W/Hg Lamp
  304
- Key Lock
  308
- Checking the Energy and Wavelength Accuracy of D2 Lamp
  309
- Checking the Energy of W Lamp
  310
- Checking the Wavelength Accuracy with Hg Lamp
  311
- Analyzing Operation
  312
- Spectrum Measurement
  312
- Setting of Recorder and Spectrum Output
  318
- Operation of Marker
  320
- Setting of Time Program
  320
- Example of Time Program Operation
  322
- Execution of Time Program
  322
- Stop of Time Program
  322
- Setups
  326
- Selection of Communication Channel
  327
- Setting of Thermostatic Flow Cell
  328
- Setting of LCD Contrast
  329
- Before Analysis
  330
- Precautions on Selection and Handling of Mobile Phase
  331
- Characteristics of Organic Solvents
  335
- Caution on Static Electricity
  339
- Degassing and Decontamination of Mobile Phase
  340
- Preparatory Operation
  340
- Degassing of Mobile Phase
  341
- Decontamination of Mobile Phase
  344
- Sample Treatment
  345
- Confirmation of Basic Actions
  346
- Maintenance and Checkup
  346
- Confirmation of Performance Specifications
  347
- Items of Performance Specification Check
  347
- Measurement of Baseline Noise
  348
- Measurement of Baseline Drift
  350
- Check of Liquid Leak Sensor
  352
- Periodic Checkup
  352
- Periodic Maintenance/Checkup
  354
Section 4

436
- Isocratic System
  439
- Outline
  439
- Low-Pressure Gradient System
  440
- Functions
  441
- Name and Function of each Part on FL Detector
  441
- Name and Function of each Part on UI Pad (Option)
  443
- Description of Fluorometry
  446
- Principle of Fluorometry
  446
- Advantages of Fluorometry
  448
- Notes on Fluorescence Analysis
  451
- Principle of Operation
  456
- Features of Light Source
  457
- Specifications
  457
- Basic Operation
  461
- Operation
  461
- Power Supply
  461
- Parameter Setting
  464
- Auto Zero Adjustment
  466
- Wavelength Setting
  466
- Flushing the Flow Cell after Use
  467
- Shutdown Procedure
  467
- Offset Setting
  468
- UTLTY Setting
  468
- Time Constant Setting
  469
- Use Time Program Setting
  470
- PMT Voltage Setting
  471
- Other Settings
  472
- GLP Functions
  477
- Xe Lamp Logbook Data Display
  477
- Xe Lamp Logbook Data Resetting
  478
- Hg Lamp Logbook Data Display
  480
- Hg Lamp Logbook Data Resetting
  481
- Key Lock Setting
  483
- Wavelength Accuracy Check by Using Hg Lamp
  484
- Xe Lamp Energy Check
  485
- Operating Instructions
  487
- Time Program Setting
  487
- Time Program Operation Examples
  493
- Time Program Starting
  493
- Time Program Stopping
  493
- Spectrum Measurement
  497
- Recorder and Spectrum Output Factors Setting
  501
- Marker Operation
  505
- Communication Channel (CH) Setting
  506
- Set up Setting
  506
- Thermo Cell Setting
  507
- Liquid Crystal Display Contrast Setting
  509
- Standard Xenon Lamp Lifetime Setting
  510
- Before Performing Analysis
  512
- Characteristics of Organic Solvents
  513
- Selection of Mobile Phase and Cautions on Handling
  513
- Cautions on Static Electricity
  520
- Degassing and Removing Dust from Mobile Phase
  521
- Degassing Mobile Phase
  521
- Preparation
  521
- Removing Dust from Mobile Phase
  524
- Sample Pretreatment
  525
- Maintenance and Troubleshooting
  527
- Operational Check
  527
- Performance Checks
  527
- Xe Lamp Energy Check
  527
- Sensitivity Check
  528
- Wavelength Accuracy Check by Using Hg Lamp
  528
- Wavelength Accuracy Check and Calibration
  541
- Leak Sensor Check
  544
- Drain Tray Check
  546
- Periodic Checks
  547
- Wavelength Accuracy Check Using Hg Lamp
  547
- Xe Lamp Energy Check
  547
- Lamp Logbook Check
  548
- Flow Cell Unit Checks
  549
- Check and Washing of Flow Cell
  550
- Disassembly and Cleaning of Flow Cell
  552
- Leakage from the Flow Cell
  555
- Cautions on Storage of the Flow Cell
  556
- Troubleshooting
  557
- Error Messages
  559
- Replacement Parts and Consumables
  562
- Spare Parts
  562
- Lamp Replacement
  564
- Xe Lamp Replacement
  564
- Hg Lamp Replacement
  578
- Fuse Replacement
  581
- Index
  582
- Appendix
  586
- Appendix 1. Installation
  586
- Unpacking
  586
- Installation Conditions
  587
- Installation Environment
  590
- Installation Place
  590
- Checking the Contents
  591
- Assembling
  592
- Removal of Transport Screws
  592
- Appendix 2. Description on Contact Signal Communication
  602
- Contact Signals Entering the E-Line Connector
  602
- Outline
  602
- Individual Contacts of each Unit
  605
- Appendix 3. Glossary
  607
- Flowpath Figures
  610
- Isocratic System
  610
- Low Pressure Gradient System
  610
Section 5

631
- About this Manual
  743
- Preface
  743
- Important
  744
- Warranty on Product
  744
- Installation, Relocation and After-Sale Technical Service
  746
- Estimated Life Time of the Instrument
  747
- Technical Seminars and Training Courses for Users
  747
- Other Precautions
  749
Section 6

755
- Functions
  757
- Specifications
  758
- Operation
  760
- Preface
  773
- About this Manual
  774
- Important
  775
- Warranty on Product
  775
- Installation, Relocation and After-Sale Technical Service
  777
- Estimated Life Time of the Instrument
  778
- Technical Seminars and Training Courses for Users
  778
- Caution on Disposal of Instrument
  779
- Disposal of this Instrument and Its Parts
  779
- Precautions on CE Conformity Marking
  779
- Information for Users on WEEE (Only for EU Countries)
  780
- Warning
  783
- Caution
  786
Section 7

791
- Isocratic System
  794
- Outline
  794
- Low-Pressure Gradient System
  795
- Functions
  797
- Name and Function of each Part on Autosampler
  797
- The Instrument Keypad
  797
- Name and Function of each Part on UI Pad (Option)
  801
- Application
  803
- Configuration of Flow Path
  803
- Operating Principle and Functional Description
  803
- Sample Injection Method
  805
- Sample Injection Procedure
  809
- Main Unit
  840
- Specifications
  840
- Thermostat (Option)
  843
- Basic Operation
  845
- Operation
  845
- Power Supply
  845
- Parameter Setting
  848
- Basics of Operation
  850
- Measuring Operation
  850
- Analyzing Operation
  856
- Shutdown Operation
  902
- Before Performing Analysis
  903
- Selection of Mobile Phase and Cautions on Handling
  905
- Degassing of Mobile Phase and Wash Fluid
  908
- Removing Dust from Mobile Phase and Wash Fluid
  909
- Characteristics of Organic Solvents
  910
- Cautions on Static Electricity
  914
- Cautions on Measurement (Handling)
  915
- Preparatory Operations
  915
- Sample Pretreatment
  916
- Preparatory Operations of Autosampler Proper
  917
- Precautions on Handling
  919
- Checking the Performance and Specifications
  922
- Maintenance
  922
- Preparation of Items
  922
- Preparation of Operation
  923
- Reproducibility Check
  926
- Cleaning
  929
- Liquid Leak Check
  929
- Liquid Leak Sensor
  929
- Periodic Maintenance
  929
- Washing the Flow Path
  929
- Waste Solution Tube
  929
- Drain Tray
  932
- Cleaning and Positioning the Wash Port
  933
- Positioning the Mechanism
  933
- Troubleshooting
  937
- Troubleshooting Table
  937
- Autosampler Main Unit Errors
  940
- Error Messages
  940
- Thermostat (Option) Errors
  943
- Consumables
  944
- Consumables and Spare Parts
  944
- Parts Replacement
  944
- Spare Parts
  945
- Fastening of SUS Pipe
  946
- Replacement of Injection Port Seal
  947
- Needle Replacement
  949
- Syringe Replacement
  951
- Discharge of Air Bubbles from Syringe
  955
- Replacing the Injection Valve Seal
  959
- Replacing the Syringe Valve Seal
  961
- Purging the Wash Pump
  963
- Discharge of Air Bubbles from Wash Pump
  964
- How to Wash Solvent Filter
  966
- How to Replace Fuse (in the Optional Thermostat)
  968
- Accessories
  969
- Thermostat
  969
- For Thermostat
  970
- Sample Racks
  970
- Standard
  970
- Vials
  971
- Degassing Unit
  982
- Optional Syringes
  982
- Index
  983
- Appendix
  987
- Appendix 1. Installation
  987
- Appendix 1.1 Unpacking
  987
- Appendix 1.2 Place for Installation
  988
- Appendix 1.3 Items to be Prepared by Customer
  990
- Appendix 1.3.1 Power Supply Arrangement
  990
- Appendix
  997
- Appendix 1.3.2 Installation Space
  997
- Checking the Contents
  997
- Appendix
  998
- Appendix 1.5.1 Removal of Transport Screws
  998
- Assembling
  998
- Appendix
  1000
- Appendix 1.5.2 Attachment of Waste Solution Tube
  1000
- Appendix
  1001
- System Power Supply
  1001
- Appendix
  1004
- System Cabling
  1004
- Appendix
  1006
- System Tubing
  1006
- Appendix
  1014
- Appendix 1.5.7 Fastening between Component Units
  1014
- GUI Controller
  1014
- Re-Transporting Method
  1015
- Appendix 2. Explanation of Contact Signals
  1017
- Appendix 2.1 Introduction
  1017
- Appendix 2.2 Contact Signals Entering E-Line Connectors
  1017
- Appendix 2.3 Individual Contacts of each Unit
  1019
- Appendix 3. Terminology
  1021

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