Flow Rate Precision Check - Hitachi CHROMASTER 5160 Instruction Manual

4.1.3 Flow Rate Precision Check

Check the precision of flow rate in the following procedure.
(1) Set the following conditions to autosampler.
STEP RACK
1 1
Press the ESC key to complete setting.
(2) Prepare methanol for the mobile phase and detergent.
Wait until the pump, detector, etc. are warmed up and the
baseline stabilizes.
(3) Press the WASH key to clean the needle and injection port,
and fill the flow path of the syringe system with the cleaning
agent.
(4) Place a vial containing at least 1 mL of standard sample in
rack position No. 1 and set the rack in the autosampler.
(5) Press the START key of autosampler to start analysis.
The analysis is carried out 6 times. The operating
conditions during analysis are indicated on the display.
Ensure beforehand that the room temperature will not
change suddenly during analysis.
(6) When the analysis is completed, the initial screen appears
and stops. If ALL END is set, ALL END screen appears and
stops. If you press the IDLE key, the initial screen appears.
4 - 5
VIALS VOL (uL)
1- 1 10.0
INJ TIME
6 3.0