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Step
Action
11
Check the O-ring in the adapter. Change it if it is damaged or
asymmetrical. Do not use a sharp tool to remove the O-ring,
as this could damage the adapter and cause leakage.
12
Insert the adapter, aligning it so that it engages the slots in
the end piece.
13
Adjust the adapter to the medium bed by clockwise rotation
of the red adjusting ring until there is no space visible between
the adapter and the filter. Check that no large air bubbles
have been trapped between the medium and the adapter. If
a large air bubble has become trapped, carefully remove and
replace the adapter.
14
Connect the column inlet tubing to the valve.
15
Start the pump and pump buffer at a flow rate of 1 to 1.5
ml/min for 5 to 10 minutes to pack the top of the medium
bed.
16
Stop the pump and disconnect the column inlet from the
valve.
17
Adjust the adapter to the filter again by clockwise rotation of
the red adjusting ring. When there is no space between the
adapter and the filter, rotate the red ring an additional half
turn for final positioning of the adapter.
18
Reconnect the column inlet to the valve.
19
Test the column performance with a suitable sample.
52-1777-00 AM
5
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