Advertisement
Step
Action
5
Carefully and slowly bring up the loose
filter by using the hook (part of the Filter
tool). Do not press or puncture the medi-
um bed.
Note:
Only a very small amount of medium
should be in suspension after removing the
filter.
6
Check that the surface of the medium bed
is horizontal and even. If not, use the
plunger (part of the Filter tool) and careful-
ly stir up 2–3 mm of medium by rotating
it on the very top of the medium bed.
7
Wet a new filter in 20% ethanol. Put the
filter into the column, avoiding air bubbles
under it. Push it gently down to the medi-
um surface with the plunger. For HR 16
columns, use the wider end of the plunger.
Note:
As the filter is inserted, any medium which
has been stirred up will be packed onto the
top of the bed.
8
Make sure the filter is horizontal, and remove the plunger.
9
Remove the liquid and traces of medium above the filter with
a pasteur-pipette. Rinse the walls and the top of the filter with
small aliquots of buffer until all the medium particles above
the filter have been removed.
10
Fill the volume above the filter with buffer.
4
52-1777-00 AM
Advertisement
