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1 Filter kit contents
These filter kits (Code Nos. 18-3575-01/18-3585-01 respectively)
contain 10 filters and 4 O-rings.
2 When to change the filter
Change the filter on the top of your column when you observe an
increased backpressure, a space which has become visible between
adapter and filter, a loss of resolution and always before you perform
a thorough column cleaning.
Change the filter only at the top since this normally restores perfor-
mance and since changing the bottom filter may lead to loss of
efficiency.
3 How to change the filter
To change the filter you need a Filter tool, Code No. 18-3590-01,
consisting of a hook and a plunger.
Step
Action
1
Start the pump and pump buffer solution, e.g., the buffer
previously used, through the column at a flow rate of
1 to 1.5 ml/min.
Note:
Do not exceed recommended maximum backpressure.
2
Gradually rotate the red adjusting ring counter-clockwise.
Unscrew the red ring completely, and stop the pump.
3
Pull the adapter out by the red ring. Disconnect the column
top inlet tubing from the valve.
4
Check that some buffer remains over the chromatography
medium bed.
52-1777-00 AM
3
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