Cleaning The Instrument After Shutting Down - Qiagen QIAcube HT User Manual

6. Remove the carriage and discard the capture plate. Rinse the carriage with clean, deionized water and dry.
7. If using plates from dedicated QIAcube HT kits, remove the channeling adapter, rinse it with clean, deionized water, and
dry.
If using Capture Plate VX or Capture Plate DX, remove the waste sink/mat support and discard the silicone mat. Rinse the
waste sink/mat support with clean, deionized water and dry.
8. Clean any reagent spilt on the instrument worktable or vacuum chamber with a damp cloth.

Cleaning the instrument after shutting down

Upon instrument shut down, the instrument cleaning procedure is automatically opened to guide the user through the instrument
cleaning process.
Additionally, the instrument cleaning procedure can be manually initiated without shutting down the instrument by clicking
the toolbar.
Perform the cleaning procedure as follows:
1. If desired, select "Run UV cleaning operation before closing?".
2. Replace lids on any tip racks containing unused tips. Discard racks containing only used tips.
3. Discard unused reagent and reagent troughs. Clean the reagent trough adapters and reagent trough lids.
4. Discard the lysis block.
5. Remove the tip chute. Clean the tip chute according to instructions in Section 7.2.5, page 156.
6. Remove the carriage and discard the capture plate. Clean carriage according to instructions in Section 7.2.5, page 156.
7. If using plates from dedicated QIAcube HT kits, leave the channeling adapter in position until the waste chamber has been flushed.
If using Capture Plate VX or Capture Plate DX, remove the silicone mat from the waste sink/mat support and discard. Leave
the waste sink/mat support in position until the waste chamber has been flushed.
8. Flush the vacuum chamber by pouring 20 mL water in the left (waste) side and clicking Next in the instrument cleaning
procedure to cycle the pump and flush the lines.
Note: The "Instrument cleaning procedure" window must be open before the water is poured into the sink.
Opening of the window automatically instructs the instrument to close the vacuum line to the elution chamber to prevent
wash water from flowing into the elution chamber and draining out through the air bleed hole onto the laboratory bench.
9. Remove and clean the waste sink/mat support or channeling adapter assembly according to instructions in Section 7.2.5,
page 156.
For information about cleaning air bleed holes, see page 156.
10. Spray the vacuum chamber and worktable with Tri-Gene from MEDI-CHEM (www.medi-chem.com) and leave for 15–
30 minutes. Wipe excess liquid with clean paper towels.
11. Discard liquid waste according to local safety regulations.
For more details, see Section 7.2, page 151.
12. Switch on the UV lamp for 30 minutes.
QIAcube HT Version 4.17 User Manual 06/2023
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