Qiagen QIAcube HT Manuals

Qiagen QIAcube HT Manuals

Manuals and User Guides for Qiagen QIAcube HT. We have 3 Qiagen QIAcube HT manuals available for free PDF download: User Manual

Qiagen QIAcube HT User Manual

Qiagen QIAcube HT User Manual (311 pages)

For QIAcube HT and QIAxtractor

Brand: Qiagen | Category: Laboratory Equipment | Size: 4.38 MB

Table of Contents

Table of Contents
3
1 Safety Information

11
- Proper Use
  11
- Electrical Safety
  13
- Biological Safety
  14
- Samples
  14
- Chemicals
  15
- Toxic Fumes
  16
- Waste Disposal
  16
- Mechanical Hazards
  16
- Maintenance Safety
  17
- Symbols on the Qiacube HT
  19
2 Introduction

21
3 General Description

25
4 Installation Procedures

45
- Instrument Delivery and Installation
  45
- Requirements
  45
- AC Power Connection
  46
- Hardware Installation
  47
- UV Lamp
  47
- HEPA Filter System
  48
- Vacuum Control Station
  48
- Pinch Valve
  49
- Vacuum Waste Hose
  49
- Software Installation
  51
- Installing the Qiacube HT Software
  51
- Connecting the Instrument to the Laptop
  59
- Connecting the Computer to the Qiacube HT
  59
- Hardware Wizard
  60
- Configuring the COM Port
  62
- Important Notes before First Use
  63
5 Qiacube HT Software

65
- Mode
  66
- Normal" Mode
  66
- Virtual" Mode
  66
- Starting the Qiacube HT Software
  67
- The Software Workspace
  70
- Virtual" Mode Indicator
  72
- Menu Bar
  72
- Toolbar
  73
- Workspace Items
  73
- Right-Hand Pane
  74
- Status Bar
  74
- Menus
  75
- File" Menu
  75
- Control" Menu
  76
- Wizards" Menu
  77
- Options" Menu
  77
- Help" Menu
  79
- Toolbar
  81
- Right-Hand Pane
  86
- Workspace Items
  87
- Plates
  88
- Plate Properties
  88
- Choosing the Plate Type
  90
- Defining Favorite Plate Types
  91
- Changing Plate Function
  92
- Tips
  93
- Tip Properties
  94
- Tip Availability
  95
- Reagents
  98
- Reagent Properties
  98
- Configuring Reagents
  100
- Required Reagent Volume
  101
- Samples and Reactions
  102
- Sample Properties
  103
- Reaction Properties
  105
- Common Properties and Operations
  109
- Entering Sample Bar Codes
  111
- Data Transfer Formats
  112
- Importing Sample and Reaction Data
  114
- Exporting Sample and Reaction Data
  119
- User Management
  125
- User Identification
  125
- User Classes
  126
- Enabling Access Control
  128
- Managing User Groups
  128
- Change Current User
  130
- Revert to First User
  131
- Calibration
  131
- Calibrating Plate Positions
  133
- Calibrating Plate Heights
  136
- Calibrating Volumes
  144
- Calibration Management
  148
- Saving Calibration Data
  148
- Reinstating Calibration Data
  150
- Deleting Calibration Data
  152
- Run Files
  153
- Working with Run Files
  154
- Template Protocol Run Files
  154
- Obtaining Run Files
  155
- Opening New or Existing Run Files
  155
- Saving Run Files
  157
- Creating a Run File with the "Vacuum Extraction" Wizard
  158
- Starting the "Vacuum Extraction" Wizard
  159
- Vacuum Extractor Configuration Wizard" Step
  159
- Table Setup" Step: Configuring the Layout
  162
- Configuration [1]" Step
  164
- Configuration [2]" Step
  165
- Load Pre-Capture Reagent 1" Step
  167
- Load Capture Plate" Step
  169
- Wash Step 1 to Wash Step N" Step
  170
- Dry Sample" Step
  172
- Elution Steps" Step
  173
- Wizard Summary
  175
- Specifying Vacuum Processing
  176
- Setting Run Parameters
  178
- Configuring Tip Air Intake
  178
- Configuring Pipet Speed
  179
- Using Liquid-Level Tracking
  180
- Configuring Error Reporting
  181
- Setting Tip Use Options
  182
- Setting the Default File Location
  183
- Resetting to Default Values
  184
- Saving Current Settings as New Defaults
  185
- Setting System Color Configuration
  186
- Setting Shutdown Beep
  189
- Setting the Screen Saver Operation
  189
- Generating Filenames for Reaction Data Export
  190
- Controlling Communication and Accessories
  190
- Selecting a COM Port
  190
- Using the Service Register
  191
- Using the UV Lamp
  194
- Using the HEPA Filter
  195
6 Operating Procedures

197
- Switching on the Qiacube HT
  197
- Assembling the Vacuum Chamber
  198
- For Plates from Qiacube HT Kits
  198
- For Capture Plate VX or Capture Plate DX
  200
- Qiacube HT Protocols
  204
- Performing a Protocol
  205
- Pre-Run Report
  207
- Pre-Run Check Errors and Warnings
  210
- In-Run Open System/Open Circuit Test
  211
- In-Run Plate Detection Test
  211
- In-Run Interactive Vacuum Steps
  212
- Modifying a Protocol Reaction List
  215
- Canceling a Protocol
  216
- Post-Run Report
  217
7 Maintenance Procedures

219
8 Troubleshooting

235
- Run Setup Errors
  235
- Important Error Messages
  235
- Software Errors
  236
- Instrument Errors
  236
- Error Codes
  237
- Technical Data
  239
- Environmental Conditions
  239
Appendix A

239
- Mechanical Data and Hardware Features
  240
- Hardware and Software Features
  242
- Waste Electrical and Electronic Equipment (WEEE
  244
- FCC Declaration
  245
- Declaration of Conformity
  246
- Liability Clause
  247
Appendix B

247
- Safety Information (French, FR
  249
- Informations de Sécurité
  249
Appendix C

249
- Utilisation Appropriée
  250
- Sécurité Électrique
  252
- Sécurité Biologique
  253
- Échantillons
  253
- Produits Chimiques
  255
- Vapeurs Toxiques
  256
- Elimination des Déchets
  256
- Dangers Mécaniques
  256
- Sécurité Relative À la Maintenance
  257
- Symboles Sur Le Qiacube HT
  259
- Safety Information (German, de
  261
- Sicherheitshinweise
  261
Appendix D

261
- Sachgemäße Handhabung
  262
- Schutz vor Stromschlag
  263
- Biologische Sicherheit
  265
- Proben
  265
- Chemikalien
  267
- Giftige Dämpfe
  267
- Entsorgen von Abfällen
  267
- Gefahren durch Mechanische Teile
  268
- Sicherheitshinweise - Wartungsarbeiten
  269
- Symbole auf dem Qiacube HT
  271
Appendix E

273
- Qiacube HT and Qiaxtractor Accessories
  273
Appendix F

275
- Advanced Settings
  275
Appendix G

285
- Sample and Reaction Functions for Non-Wizard Protocols
  285
- Description of the Generic XML Format
  303
Appendix H

303

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Qiagen QIAcube HT User Manual

Qiagen QIAcube HT User Manual (282 pages)

For use with the QIAcube HT Prep Manager Software

Brand: Qiagen | Category: Medical Equipment | Size: 42.31 MB

Table of Contents

Table of Contents
2
1 Introduction

10
- Provided User Manuals
  11
- About this User Manual
  11
- General Information
  12
  - Technical Assistance
    12
  - Policy Statement
    12
  - Version Management
    12
- Intended Use of the Qiacube HT
  12
- Requirements for Qiacube HT Users
  13
- Requirements for some Kits
  13
2 Safety Information

14
- Proper Use
  15
- Electrical Safety
  17
- Biological Safety
  18
  - Samples
    18
- Chemicals
  19
  - Toxic Fumes
    19
- Waste Disposal
  19
- Mechanical Hazards
  19
- Maintenance Safety
  20
- Symbols on the Qiacube HT
  23
3 General Description

24
- Qiacube HT Instrument
  26
  - Lid
    27
  - Feet
    27
  - Tip Chute
    28
  - Tip Disposal Box
    28
  - HEPA Filter
    29
  - UV Lamp
    29
  - Pipettor Head and Robotic Arm
    30
  - Worktable
    30
  - Accessory Panel
    32
  - Vacuum Chamber
    33
  - Vacuum Control Station and Waste Bottle
    35
  - Pinch Valve
    37
  - Internal Features of the Qiacube HT
    39
- Computer
  41
4 Installation Procedures

43
5 Getting Started

61
- Start the Software
  62
- Customizing Global Settings
  63
- Customize User Settings
  67
- Managing Users
  69
  - Changing Password
    75
- Managing Labware
  76
- Assembling the Vacuum Chamber
  78
  - Assembly for Calibration
    79
  - Assembly for Standard Purification Protocols
    81
- Instrument Calibration
  83
  - Calibrate Volumes
    85
  - Calibration Positioning Buttons
    87
  - Calibrate Tip Racks
    88
  - Calibrate Labware
    90
    
    Select Labware to Calibrate
    90
    
    Prepare Worktable for Labware Calibration
    94
    
    Calibrate X- and Y-Axis
    96
    
    Calibrate Z-Axis
    98
  - Saving and Importing Calibration Data
    100
6 Operating Procedures

102
- Starting a Run
  103
  - Setup Step
    104
  - Labware Selection Step
    106
    
    Select Other Input Labware
    109
  - Assignment
    112
    
    Assigning Samples
    113
    
    Removing Assignment
    114
    
    Blocking Output Positions
    114
  - Worktable Step
    114
    
    Preparing the Worktable
    117
    
    Checking the Waste Bottle Status
    118
    
    Preparing Tip Chute and Tip Disposal Box
    119
    
    Preparing the Vacuum Chamber
    121
    
    Loading Tips
    121
    
    Loading Reagents and Samples
    124
    
    Starting a Run
    125
  - Run Step
    125
    
    Open System Test
    126
    
    Plate Detection Test
    126
    
    Pausing/Stopping a Run
    127
    
    Vacuum Performance Check
    128
    
    Run Completion
    130
- Finishing Work and Shutting down
  131
7 Qiacube HT Prep Manager Software

133
- Basic Concepts and General Software Usage
  134
  - Concepts
    134
    
    User Roles Concept
    134
    
    Experiments and Templates
    134
    
    Protection of Files
    137
  - General Software Usage
    137
    
    Controlling the Mouse
    137
    
    Use of Color
    138
    
    Displaying Errors and Warnings
    139
    
    Entering Data
    140
    
    Working with Tables
    142
    
    Selecting Samples
    143
  - General Elements
    145
    
    Menu
    145
    
    Main Toolbar
    148
    
    Button Bar
    150
    
    Status Bar
    150
    
    File Dialog
    151
    
    Scan Plate Barcode Dialog
    155
  - Environments
    155
    
    Home
    156
    
    Experiments
    157
    
    Service
    159
    
    Configuration
    160
  - Getting Help
    161
- Working with Experiments
  163
  - Manage Experiments and Templates
    163
    
    Create a Template
    163
    
    Manage Favorite Templates
    164
    
    View Recent Experiments
    166
  - Creating an Experiment
    167
    
    Create an Empty Experiment
    168
    
    Create an Experiment Using a Template
    169
    
    Create an Experiment from an Existing Experiment
    171
- Report
  171
- Administrative Tasks
  176
  - Configuring Qiacube HT
    176
  - Viewing Software Version
    176
  - View System Audit Trail
    176
8 Maintenance Procedures

179
- Cleaning Agents
  180
- Servicing
  182
- Regular Maintenance
  182
  - Cleaning the Instrument after Completing a Run
    182
  - Cleaning the Instrument before Shutting down
    183
  - Cleaning the Lid
    185
  - Emptying the Waste Bottle
    185
  - Cleaning Accessories
    186
  - Using UV Lamp
    188
- Periodic Maintenance
  189
9 Troubleshooting

199
- Support Packages
  201
- System Setup
  204
- Operation
  207
- Self-Diagnostic Tools
  209
  - Connection Check
    210
  - Cycle Pipettor Head
    210
  - Check for Loss of Steps
    211
  - Check Vacuum Pressure
    211
  - Check for Home-Opto Alignment
    212
10 Glossary

213
11 Appendices

219
- 11.1 Appendix A
  220
- Environmental Conditions
  220
  - Waste Electrical and Electronical Equipment
    221
  - FCC Declaration
    222
  - Declaration of Conformity
    222
  - Liability Clause
    223
- Technical Data
  220
- Appendix B
  
  223
  - Utilisation Appropriée
    224
  - Sécurité Électrique
    226
  - Sécurité Biologique
    227
    
    Échantillons
    227
  - Produits Chimiques
    228
    
    Vapeurs Toxiques
    228
  - Traitement des Déchets
    228
  - Dangers Mécaniques
    229
  - Sécurité Relative À la Maintenance
    230
  - Symboles Sur Le Qiacube HT
    232
- Appendix C Consignes de Sécurité
  
  223
- Appendix D Sicherheitshinweise
  
  233
  - Sachgemäße Handhabung
    233
  - Elektrische Sicherheit
    235
  - Biologische Sicherheit
    236
    
    Proben
    236
  - Chemikalien
    236
    
    Giftige Dämpfe
    237
  - Abfallentsorgung
    237
  - Gefahren durch Mechanische Teile
    237
  - Sicherheitshinweise - Wartungsarbeiten
    238
  - Symbole auf dem Qiacube HT
    241
  - Qiacube HT Accessories
    242
- Appendix E
  
  242

Qiagen QIAcube HT User Manual

Qiagen QIAcube HT User Manual (207 pages)

Brand: Qiagen | Category: Laboratory Equipment | Size: 15.56 MB

Table of Contents

Table of Contents
2
1 Introduction

10
2 Safety Information

13
- Proper Use
  13
- Electrical Safety
  14
- Biological Safety
  15
  - Samples
    15
- Chemicals
  16
  - Toxic Fumes
    16
- Waste Disposal
  16
- Mechanical Hazards
  16
- Maintenance Safety
  17
- Symbols on the Qiacube HT
  18
3 General Description

19
- The Qiacube HT
  19
  - Instrument Lid
    20
  - UV Lamp
    20
  - Pipetting Head
    21
  - Tip Chute
    21
  - HEPA Filter
    21
  - Accessory Panel
    22
  - Feet
    22
  - Worktable
    22
  - Principles of Operation
    23
- Instrument
  25
4 Installation Procedures

32
- Instrument Delivery and Installation
  32
- Requirements
  32
  - Site
    32
  - Unpacking the Instrument
    33
  - AC Power Connection
    33
  - Hardware Installation
    34
    
    HEPA Filter System
    34
    
    UV Lamp
    34
    
    Vacuum Control Station
    34
    
    Pinch Valve
    35
    
    Vacuum Waste Hose
    35
  - Software Installation
    37
    
    Installing the Qiacube HT Software
    37
  - Connecting the Instrument to the Laptop
    43
    
    Connecting the Computer to the Qiacube HT
    43
  - Hardware Wizard
    44
  - Configuring the COM Port
    45
  - Important Notes before First Use
    46
5 Qiacube HT Software

47
- Mode
  47
- Normal" Mode
  47
- Virtual" Mode
  47
- Starting the Qiacube HT Software
  48
- The Software Workspace
  51
- Virtual" Mode Indicator
  52
  - Menu Bar
    52
  - Toolbar
    52
  - Workspace Items
    53
  - Right-Hand Pane
    53
  - Status Bar
    54
- Menus
  54
- File" Menu
  54
- Control" Menu
  55
- Wizards" Menu
  55
- Options" Menu
  56
- Help" Menu
  56
- Toolbar
  58
- Right-Hand Pane
  60
- Workspace Items
  61
- Plates
  61
  - Plate Properties
    61
  - Choosing the Plate Type
    62
  - Defining Favorite Plate Types
    63
  - Changing Plate Function
    64
- Tips
  65
  - Tip Properties
    65
  - Tip Availability
    66
  - Reagents
    68
    
    Reagent Properties
    68
    
    Configuring Reagents
    69
    
    Required Reagent Volume
    70
  - Samples and Reactions
    71
    
    Sample Properties
    72
    
    Reaction Properties
    73
    
    Common Properties and Operations
    76
    
    Entering Sample Bar Codes
    76
- Data Transfer Formats
  77
- Importing Sample and Reaction Data
  78
- Exporting Sample and Reaction Data
  81
- User Management
  85
  - User Identification
    85
  - User Classes
    85
  - Enabling Access Control
    87
  - Managing User Groups
    87
  - Change Current User
    88
  - Revert to First User
    89
- Calibration
  89
  - Calibrating Plate Positions
    90
  - Calibrating Plate Heights
    92
  - Calibrating Volumes
    97
  - Calibration Management
    100
  - Saving Calibration Data
    100
  - Reinstating Calibration Data
    102
  - Deleting Calibration Data
    103
- Run Files
  104
- Working with Run Files
  104
  - Template Protocol Run Files
    104
  - Obtaining Run Files
    105
  - Opening New or Existing Run Files
    105
  - Saving Run Files
    106
  - Creating a Run File with the "Vacuum Extraction" Wizard
    107
  - Starting the "Vacuum Extraction" Wizard
    108
  - Vacuum Extractor Configuration Wizard" Step
    108
  - Configuration [1]" Step
    111
  - Configuration [2]" Step
    112
  - Load Pre-Capture Reagent 1" Step
    113
  - Load Capture Plate" Step
    115
  - Wash Step 1 to Wash Step N" Step
    115
  - Dry Sample" Step
    117
  - Elution Steps" Step
    117
    
    Wizard Summary
    119
    
    Specifying Vacuum Processing
    120
    
    Configuring Tip Air Intake
    121
    
    Setting Run Parameters
    121
    
    Configuring Pipet Speed
    122
    
    Using Liquid-Level Tracking
    123
    
    Configuring Error Reporting
    124
    
    Setting Tip Use Options
    124
    
    Setting the Default File Location
    126
    
    Resetting to Default Values
    127
    
    Saving Current Settings as New Defaults
    127
    
    Setting System Color Configuration
    127
    
    Controlling Communication and Accessories
    130
    
    Generating Filenames for Reaction Data Export
    130
    
    Selecting a COM Port
    130
    
    Setting Shutdown Beep
    130
    
    Setting the Screen Saver Operation
    130
    
    Using the Service Register
    131
    
    Using the UV Lamp
    133
    
    Using the HEPA Filter
    134
6 Operating Procedures

135
- Switching on the Qiacube HT
  135
- Assembling the Vacuum Chamber
  135
  - For Plates from Qiacube HT Kits
    135
  - For Capture Plate VX or Capture Plate DX
    137
- Qiacube HT Protocols
  140
  - Performing a Protocol
    140
  - Pre-Run Report
    142
  - Pre-Run Check Errors and Warnings
    144
  - In-Run Open System/Open Circuit Test
    144
  - In-Run Plate Detection Test
    145
  - In-Run Interactive Vacuum Steps
    146
  - Modifying a Protocol Reaction List
    148
- Canceling a Protocol
  148
- Post-Run Report
  149
7 Maintenance Procedures

151
- Servicing
  151
- Annual Preventive Maintenance
  151
- Regular Maintenance Procedures
  151
- Weekly Maintenance Procedure
  158
- Pipetting Head Service Kit
  159
- Replacing HEPA Filters
  159
- Replacing Filters on the Vacuum Station
  160
- Shutting down the Qiacube HT
  161
8 Troubleshooting Guide

162
- Run Setup Errors
  162
- Important Error Messages
  163
  - Software Errors
    163
  - Instrument Errors
    163
  - Error Errors
    164
9 Technical Specifications

165
- Environmental Conditions
  165
  - Operating Conditions
    165
  - Transportation Conditions
    165
  - Storage Conditions
    165
- Mechanical Data and Hardware Features
  166
  - Instrument Dimensions and Weight
    166
  - Worktable Capacity
    166
  - Liquid Handling
    166
    
    Hardware and Software Features
    167
    
    HEPA Filter Unit
    167
    
    Instrument Lid
    167
  - Tip Pickup Mode
    169
  - Detect Tip Absence
    169
Appendix A Advanced Settings

169
Appendix B

189
- Declaration of Conformity
  189
- Waste Electrical and Electronic Equipment (WEEE)
  189
- FCC Declaration
  190
- Liability Clause
  190
Appendix C - Ordering Information

191
Appendix D - Informations de Sécurité

192
- Utilisation Appropriée
  192
- Sécurité Électrique
  194
- Sécurité Biologique
  195
  - Échantillons
    195
- Produits Chimiques
  195
  - Vapeurs Toxiques
    196
- Elimination des Déchets
  196
- Dangers Mécaniques
  196
- Sécurité Relative à la Maintenance
  197
- Symboles Sur le Qiacube HT
  197
Appendix E - Sicherheitshinweise

198
- Sachgemäße Handhabung
  199
- Schutz vor Stromschlag
  200
- Biologische Sicherheit
  201
  - Proben
    201
- Chemikalien
  202
  - Giftige Dämpfe
    202
- Entsorgen von Abfällen
  202
- Gefahren durch Mechanische Teile
  202
- Sicherheitshinweise - Wartungsarbeiten
  203
- Symbole auf dem Qiacube HT
  204
- Document Revision History
  205

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