Calibrate Incubator Oxygen Sensor - Thermo Scientific invitrogen EVOS OSI-2 User Manual

Chapter 2 Methods
2
Use the Onstage Incubator with the EVOS
3. Select the appropriate options for the Gas Inputs that reflect the set-up for the EVOS
Incubator (OSI-2):
• Port 1: Select Air or Premix, if not using the internal air compressor.
If you select Premix, manually enter the percentage of the CO
of the set‑up.
• Port 2: Reserved for N
• Port 3: Reserved for CO
4. Click Apply after you have configured the gas connections for each port.
5. Open the gas cylinder valve, taking care to shut off or turn down the output pressure before
opening.
Note: The right gauge on the regulator shows the input pressure (tank pressure) and the left gauge
shows the output pressure. After the cylinder valve is opened, the output pressure can be adjusted
up to 40–50 psi and, if applicable, the output shut-off valve can be opened.
6. Set the gas flow on the regulators:
• Air: 40–50 psi
• CO
: 40–50 psi
2
• N
: 40–50 psi
2
IMPORTANT! Do not exceed 50 psi of pressure. Exceeding 50 psi will damage the incubator.
Going below 40 psi will result in suboptimal performance.

Calibrate incubator oxygen sensor

Calibrating the oxygen sensor ensures that the atmosphere in the environmental chamber is replenished
with the appropriate gasses in the correct proportion.
Note: We recommend calibrating the oxygen sensor every 6 months or whenever precise control of
oxygen is needed (e.g., for hypoxia experiments). The OSI-2 provides a notification if the O
not been calibrated in more than 6 months.
1. Go to the Settings tab, then click Incubator to expand the Incubator panel.
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M5000 Imaging System
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only.
2
only.
2
and O to reflect the specifics
2 2
EVOS Onstage Incubator (OSI-2) User Guide
™
Onstage
™
sensor has
2