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Translation of the original operating manual 10/2013 Rev. 2.20 of 28/04/2022 Sigma 4-5L;Laboratory Centrifuge;Sigma 4-5L IVD Laboratory Centrifuge part no. 0701502 Sigma 4-5L Sigma 4-5L IVD From serial no. 146008 Laboratory Centrifuge Operating Manual Please retain for later use!
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 2 / 82 Translation of the original operating manual, part no. 0701502...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Table of contents General information ......................9 Importance of the operating manual .................. 9 Intended use ........................9 Warranty and liability......................9 Copyright ........................10 Standards and regulations ....................10 Scope of supply ......................10 Layout and mode of operation ..................
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Table of contents Storage and transport ....................... 28 Dimensions and weight ....................28 Storage conditions ......................28 Notes on transport ......................28 Packaging ........................29 Transport safety device ....................29 Set-up and connection ...................... 30 Installation site ........................
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Table of contents Malfunctions and error correction ..................48 General malfunctions ...................... 48 7.1.1 Emergency lid release ....................49 Table of error codes ......................50 Service contact ....................... 51 Maintenance and service ....................52 Maintenance ........................
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Table of contents Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 8 / 82 Translation of the original operating manual, part no. 0701502...
• compliance with the inspection and maintenance instructions. Sigma Laborzentrifugen GmbH cannot be held liable for: • damage resulting from the improper use of the centrifuge not in line with its intended purpose, •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 1 General information → Copyright The copyright concerning the operating manual remains with Sigma Laborzentrifugen GmbH. The operating manual is solely intended for the operator and their personnel. It includes instructions and information that must not be •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 2 Layout and mode of operation → Layout and mode of operation → Layout of the centrifuge 2.1.1 Functional and operating elements User interface (see chapter 6.3.1 - "User interface") Mains switch Fig.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 2 Layout and mode of operation 2.1.2 Name plate Manufacturer Power consumption Max. speed Max. kinetic energy Serial number Part number Nominal voltage Type Consult operating manual 10 Symbol for special disposal (see chapter 9 - "Disposal")
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 2 Layout and mode of operation → Mode of operation → 2.2.1 Centrifugation principle Centrifugation is a process for the separation of heterogeneous mixtures of substances (suspensions, emulsions, or gas mixtures) into their components.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 2 Layout and mode of operation → 2.2.2.1 Speed, radius, and relative centrifugal force The acceleration g, which the samples are subject to, can be increased by increasing the radius in the rotor chamber and by increasing the speed.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Safety → Marking of the unit The following symbols are used on this centrifuge: Arrow indicating the direction of On (Power) rotation Rotor loading information Off (Power) (see chapter 2.1.2 - "Name plate")
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Explanation of the symbols and notes In this operating manual, the following names and symbols to indicate hazards are used: This symbol stands for a direct hazard to the life and health of persons.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Responsibility of the operator The operator undertakes to authorise only trained, specialised personnel to work on the centrifuge (see chapter 3.4 - "Requirements concerning the personnel"). The areas of responsibility of the personnel concerning the operation, maintenance, and care of the unit must be clearly defined.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Requirements concerning the personnel Risk of injury if the personnel are not sufficiently qualified If unqualified personnel perform work on the centrifuge or are present in the danger zone of the centrifuge, hazards result that can cause serious injuries and considerable damage to property.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety The operating personnel must ensure that any serious event which either directly or indirectly had, could have had or could have one of the following consequences will be reported to the manufacturer or competent authority:...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Safety instructions 3.6.1 Electrical safety As protection against electric shock, the centrifuge is equipped with an earthed mains power cable and connector. To ensure the effectiveness of this safety feature, the following must be ensured: •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety • Do not spin any substances that could damage the material of the rotors and buckets of the centrifuge in any way. Highly corrosive substances, for example, damage the material and affect the mechanical strength of the rotors and buckets.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → 3.6.5 Safety instructions for centrifugation For safe operation, observe the following before starting the centrifuge: • Ensure that the centrifuge was set up properly (see chapter 5 - "Set-up and connection").
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety 3.6.7 Service life (for centrifuges according to Machinery Directive) The rotors and accessories have a limited service life. • Perform regular checks (at least once per month) for safety reasons! •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety 3.6.8 Service life (for centrifuges according to the IVD regulation) Centrifuge The service life of the centrifuge depends on several factors, e.g. the type and frequency of use, the area of application and the performance of service and maintenance tasks.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety 3.6.9 Safety of rotors and accessories 3.6.9.1 Marking During production, every rotor and bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection. Some rotors also have an additional serial number providing further detailed information.
(see chapter 7.2 - "Table of error codes"). 3.7.4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument. Please contact the Sigma service department (see chapter 7.3 - "Service contact"). → 3.7.5 Imbalance monitoring system The indication "Imbalance"...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 3 Safety → Measures in the event of hazards and accidents • If an emergency arises, switch off the centrifuge immediately! • If in doubt, call the emergency doctor! DANGER →...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 4 Storage and transport → Storage and transport → Dimensions and weight 4-5L, 4-5L IVD Height: 390 mm Height with open lid: 880 mm Width: 496 mm Depth: 634 mm Weight: 77 kg →...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 4 Storage and transport Packaging The centrifuge is packaged in a wooden crate. • After taking off the lid, remove the side panels. • Remove the packaging material. • Lift the centrifuge upwards with a lifting device to lift it safely. When lifting the centrifuge, always reach under the centrifuge from the side.
CAUTION Sigma centrifuges are units of protection class I. The centrifuges of this model series have a three-wire power cord with an IEC C13 connector. The removable power cord must not be longer than 3 m!
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 5 Set-up and connection The centrifuges are equipped with a mains power switch with an integrated thermal circuit breaker. • Switch the unit off by actuating the mains power switch. •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → Using the centrifuge → Initial start-up • Before the initial start-up, please ensure that your centrifuge is properly set up and installed (see chapter 5 - "Set-up and connection").
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.2.2 Installation of rotors and accessories → 6.2.2.1 Installation of the rotor • Open the centrifuge lid by pressing the lid key. • Unscrew the rotor tie-down screw from the motor shaft (counter- clockwise).
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge 6.2.2.2 Installation of accessories • Only use inserts that are suitable for the rotor. • All buckets of the swing-out rotor need to be installed when spinning.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge Pay attention to the marking of the centrifuge (see the illustration below)! Safety indications on the centrifuge must be kept readable at all times. If necessary, they must be replaced.
Start key Function knob Display Stop key Lid key Fig. 11: User interface of the “Spincontrol L” control system (here: Sigma 4-5L) Display The centrifuge display has the following display fields: Speed/rcf field Various display fields (e.g. for rotor,...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.3.2 Manual mode → 6.3.2.1 Starting a centrifugation run The centrifuge is ready for operation when the start key is illuminated. • Press the start key in order to start a centrifugation run.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.3.2.5 Speed / relative centrifugal force (RCF) The set speed of the centrifuge is displayed in the upper area of the Speed/RCF field. The actual value is displayed right below. The speed is...
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge Runtime as of the set speed If the runtime is to be counted as of the moment when the set speed is reached, the symbol (see the illustration) behind the set speed value must be activated: •...
6.3.2.7 Temperature monitoring function ("Temp") The 4-5L IVD includes a special feature for monitoring the temperature in the rotor chamber. It is also possible to define an alarm temperature. The sole purpose of the temperature display is to monitor the temperature in the rotor chamber.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge Fig. 20: Display with an activated temperature monitoring function If the preset alarm temperature is reached or exceeded during a centrifugation run, the actual temperature inside the rotor chamber will be displayed in a flashing manner in the lower area.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge Automatic rotor identification system If the centrifuge is equipped with an automatic rotor identification system, the input mode will be activated automatically if the system detects a different rotor with several different types of possible buckets than the rotor that is set.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.3.2.10 Automatic lid opening function ("AutoOpen") The automatic lid opening function must be activated so that the lid opens automatically at the end of the operation.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge 6.3.2.12 Program lock ("ProgLock") When the program lock is active, it is impossible to save any new programs. In this case, the function "save program" is disabled.
WARNING The cycle display will be reset after the rotor and buckets have been replaced by the service department of Sigma Laborzentrifugen GmbH (see chapter 7.3 - "Service contact"). 6.3.2.14 Input lock In order to prevent the centrifuge from being manipulated by unauthorised persons, inputs can be disabled via the menu.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.3.3 Program mode A program contains all the data that are required for a centrifuge run. Certain sedimentation results can be repeated under identical conditions. Programs can be saved, loaded, executed, and edited, when the centrifuge is at a standstill.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 6 Using the centrifuge → 6.3.3.2 Loading a program • Select the menu item "run Prog" and confirm the selection. The indication "run" flashes in the activated status. • Select the desired program and confirm the selection by pressing the function knob.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 7 Malfunctions and error correction → Malfunctions and error correction → General malfunctions Error messages are displayed as "Error" followed by a code number. If the acoustic signal is activated, it sounds when the error message is displayed.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 7 Malfunctions and error correction 7.1.1 Emergency lid release In the event of a power failure, it is possible to manually open the centrifuge lid. • Switch off the mains power switch and disconnect the power cord from the socket.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 7 Malfunctions and error correction → Table of error codes Error no. Kind of error Measures Note • System error Allow to slow down All these errors stop the centrifuge or cause it to •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 7 Malfunctions and error correction → Service contact In the event of queries, malfunctions, or spare part enquiries: From Germany: Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode (Germany) Tel.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → Maintenance and service The centrifuge, rotor, and accessories are subject to high mechanical stress. Thorough maintenance performed by the user extends the service life and prevents premature failure.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → 8.1.2 Accessories For the care of the accessories, special safety measures must be considered as these are measures that will ensure operational safety at the same time! CAUTION •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → 8.1.3 Rotors, buckets and carriers Rotors, buckets and carriers are produced with the highest precision, in order to withstand the permanent high stress from high gravitational fields.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → 8.1.4 Load bearing bolts Only greased load-bearing bolts ensure a uniform swing-out of the buckets and, therefore, the smooth operation of the centrifuge. Load-bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → 8.1.5 Glass breakage In the case of glass breakage, immediately remove all glass particles (e.g. with a vacuum cleaner). Replace the rubber cushions since even thorough cleaning will not remove all glass particles.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → 8.2.1 Autoclaving The service life of the accessories essentially depends on the frequency of autoclaving and use. • Replace the accessories immediately when the parts show changes in colour or structure or in the occurrence of leaks etc.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → Service In the event of service work that requires the removal of the panels, there is a risk of electric shock or mechanical injury. • Only qualified specialist personnel is authorised to perform this service work.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 8 Maintenance and service → Return of defective centrifuges or parts Although we exercise great care during the production of our products, it may be necessary to return a unit or accessory to the manufacturer.
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NOTE The forms can be downloaded online from www.sigma-zentrifugen.de → [Service] → [Overhaul and repair]. Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 60 / 82 Translation of the original operating manual, part no. 0701502...
Disposal → Disposal of the centrifuge In accordance with the directive 2012/19/EU, SIGMA centrifuges are marked with the symbol shown to the left. This symbol means that it is not permissible to dispose of the unit among household waste. •...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 10 Technical data 10.1 Ambient conditions • The figures are valid for an ambient temperature of +23°C and a nominal voltage 10 %. At a nominal voltage of 100V or 200V, a tolerance of +10% / -5% applies.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix → 11 Appendix → 11.1 Range of accessories The complete list of accessories can be downloaded from www.sigma-zentrifugen.de. Some accessories come supplied together with a data sheet that includes important information and notes on safety.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix → 11.3 Acceleration and deceleration curves Linear curves are numbered in the direction of increasing acceleration (from right to left). The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers.
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix → 11.4 Table of the service life of rotors and accessories • The rotors and accessories must be put out of service after 10 years. Any use after this period may be permissible in individual cases after an inspection performed by the manufacturer.
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix → Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 68 / 82 Translation of the original operating manual, part no. 0701502...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix 11.5 Resistance data The data refer to resistance at 20°C. NOTE - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Acetaldehyde Acetamide...
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Chromic acid Chromic potassium KCr(SO saturated sulphate Citric acid Citric acid Copper sulphate...
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Isopropyl alcohol Lactic acid Magnesium chloride MgCl Mercuric chloride HgCl Mercury Methyl acetate...
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix - no data 1 resistant 2 practically resistant 3 partially resistant 4 not resistant Medium Formula Sodium bisulphite NaHSO Sodium carbonate Sodium chloride NaCl Sodium hydroxide NaOH Sodium hydroxide...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix Declaration of conformity – China RoHS 2 11.7 Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 77 / 82 Translation of the original operating manual, part no. 0701502...
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD 11 Appendix Version 10/2013, Rev. 2.20 of 28/04/2022 • sb 78 / 82 Translation of the original operating manual, part no. 0701502...
Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Index 12 Index Changes in structure ........57 Chemical and biological safety ....21 Acceleration ..........42 Chemical resistance of plastic ....53 Acceleration curves ......42, 66 China RoHS 2 – Declaration of conformity . 77 Accessories, cleaning and care ....
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Index Mode of operation ........13 Radius ............14 Motor error ..........50 Reaching the maximum number of cycles .. 44 Motor shaft ........... 33, 52 Relative centrifugal force (RCF) ... 14, 38 Multiple carrier ...........
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Laboratory Centrifuge Sigma 4-5L Laboratory Centrifuge Sigma 4-5L IVD Index Service life of the accessories ....57 Table of rotors and accessories with a different service life ....23, 24, 67 Service life of the centrifuge ....... 24 Technical data ........... 62 Service work ..........
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