Biotek Synergy H1 Operator's Manual
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Biotek Synergy H1 Operator's Manual

Hybrid multi-mode microplate reader
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  • Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual...
  • Page 3 Synergy H1™ Hybrid Multi-Mode Microplate Reader Operator’s Manual March 2014 © 2014 Part Number 8041000 Revision G ® BioTek Instruments, Inc.
  • Page 4 BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 5: Table Of Contents

    13: Run a System Test ..............19 14: Test the Injector System ............20 Operational/Performance Qualification ..........22 Repackaging and Shipping Instructions ..........22 Getting Started .................. 29 Modular Design ................30 External Components ..............31 Internal Components ..............31 Synergy H1 Operator’s Manual...
  • Page 6 Absorbance Specifications ............. 144 Dispense/Read Specifications ............145 Fluorescence Specifications (Mono-Based) ........145 Fluorescence Specifications (Filter-Based) ........146 Luminescence Specifications ............147 Error Codes ..................149 Overview ..................150 Error Codes ................. 151 Instrument Dimensions for Robotic Interface ......... 155 BioTek Instruments, Inc.

  • Page 7: Global Service And Support

    Global Service and Support  See also Product Support & Service on page 6. BioTek instrument service and repair is available worldwide at one of BioTek's International Service Centers and in the field at your location. For technical assistance, contact the Technical Assistance Center (TAC) at BioTek World Headquarters US. To arrange for service or repair of your instrument, contact the office nearest you.
  • Page 8 Phone: +41 (41) 2504060 Fax: +82 (0) 2-562-4750 Fax: +41 (41) 2505064 Email: korea@biotek.com Email: info@biotek.ch Website: www.biotekinstruments.co.kr Website: www.biotek.ch BioTek United Kingdom (UK) 6 Bull Street Potton, Bedfordshire SG19 2NR United Kingdom Phone: +44 (1767) 262000 Fax: +44 (1767) 262330 Email: info@biotek.uk.com Website: www.biotek.uk.com...

  • Page 9: Revision History

    Appendix A: Specifications: Corrected Incubation specification. 11/11 Chapter 2: Installation: Updated the Carrier Shipping Bracket photo in Figure 1. Updated the BioTek part number for the shipping hardware. Updated the Dispense Module installation instructions. Chapter 7: Instrument Qualification: For the Absorbance Plate Test, removed the restriction on the use of the peak closest to 243 nm for the Erbium glass (any peak may be used).
  • Page 10 Chapter 7, Instrument Qualification: Added “filter-based” to TRF descriptions. Appendix A, Specifications: Added information about the incubation gradient. 3/2014 Chapter 4, Filters and Mirrors: Add photos to procedure for installing the filter cube; added a table of mirrors with their wavelength ranges. BioTek Instruments, Inc.

  • Page 11: Document Conventions

    Intended Use Statement • The Synergy H1 is a hybrid multi-mode microplate reader. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s).

  • Page 12: Quality Control

    Warranty and Product Registration Take a moment to review the warranty information that shipped with your product. Please also register your product with BioTek to ensure that you receive important information and updates about the product(s) you have purchased. You can register online through the Customer Resource Center (CRC) at www.biotek.com or by calling 888-451-...

  • Page 13: Hazards

    If a spill occurs while a program is running, abort the program and turn the instrument off. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid. Contact BioTek TAC for assistance. Failure to operate the equipment according to the Warning! Unspecified Use.

  • Page 14: Precautions

    Keep hands/fingers clear of these areas when the instrument is operating. Precautions The following precautions are provided to help avoid damage to the instrument: . The instrument should be serviced by BioTek-authorized Caution: Service personnel. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.

  • Page 15: Ce Mark

    EN 61010-1, “Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements.” EN 61010-2-081, “Requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.” EN 61010-2-010, “Particular requirements for laboratory equipment for the heating of materials.” Synergy H1 Operator’s Manual...

  • Page 16: Electromagnetic Interference And Susceptibility

    Communications. Le present appareil numerique n'émet pas de bruits radioélectriques depassant les limites applicables aux appareils numérique de la Class A prescrites dans le Réglement sur le brouillage radioélectrique édicté par le ministère des Communications du Canada. BioTek Instruments, Inc.

  • Page 17: User Safety

    Part 1: general requirements.” • Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: general requirements.” • EN 61010 standards, see starting on page xiii. CE Mark Synergy H1 Operator’s Manual...

  • Page 18: Safety Symbols

    Attenzione, rischio di schiacciare ed intrappolarsi Warning, hot surface Warning, potential biohazards Attention, surface chaude Attention, risques biologiques potentiels Warnen, heiße Oberfläche Warnung! Moegliche biologische Giftstoffe Precaución, superficie caliente Atención, riesgos biológicos Attenzione, superficie calda Attenzione, rischio biologico BioTek Instruments, Inc.
  • Page 19 Rayonnement laser: Ne pas regarder dans le faisceau Gebrauchsanweisung beachten Laserstrahlung: Nicht in den strahl blicken Consultar las instrucciones de Radiación de láser: No mire fijamente al rayo Consultare le istruzioni per uso Radiazione di laser: Non stare nel fascio Synergy H1 Operator’s Manual...
  • Page 20 | Safety Symbols BioTek Instruments, Inc.

  • Page 21: Introduction

    Chapter 1 Introduction This chapter introduces the Synergy H1 Hybrid Multi-Mode Microplate Reader, describes its hardware and software features, and provides contact information for technical assistance. Product Description ............2 Package Contents & Accessories .......... 3 Optional Accessories ............4 Product Support &...

  • Page 22: Product Description

    The Synergy H1 supports the reading of 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry as well as the Take3 and Take3 Trio Micro-Volume Plate.

  • Page 23: Package Contents & Accessories

    Package Contents & Accessories | Package Contents & Accessories  Package contents and part numbers are subject to change. Please contact BioTek Customer Care with any questions. Item Part # Synergy H1 Operator’s Manual 8041000 Power supply 76061 Power cord set (specific to installation environment):...

  • Page 24: Optional Accessories

    Call BioTek Sales Additional filters and filter cubes; contact BioTek for part numbers and availability The Synergy H1 is compatible with the BioStack Microplate Stacker. The BioStack rapidly and systematically transfers a “stack” of microplates to and from the instrument’s microplate carrier.
  • Page 25 Solutions for Liquid Tests (see Chapter 7) Part # Absorbance Liquid Test Solutions: BioTek Wetting Agent Solution 7773002 BioTek QC Check Solution No. 1 (25 mL) 7120779 BioTek QC Check Solution No. 1 (125 mL) 7120782 Phosphate-Buffered Saline (PBS) Tablets (pH 7.2–7.6) Sigma #P4417 β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form)

  • Page 26: Product Support & Service

    If your instrument or software fails to function properly, if you have questions about how to use or maintain our products, or if you need to send an instrument to BioTek for service or repair, please contact our Technical Assistance Center (“TAC”).

  • Page 27: I Nstallation

    Chapter 2 I nstallation This chapter includes instructions for unpacking and setting up the Synergy H1 and, if applicable, the external dispense module. Instructions are also included for preparing the reader and dispense module for shipment. Product Registration ............8 Important Information ............

  • Page 28: Product Registration

    | Chapter 2: Installation Product Registration Please register your product(s) with BioTek to ensure that you receive important information and updates about the product(s) you have purchased. Register online through BioTek’s Customer Resource Center (CRC) at www.biotek.com or by contacting BioTek Customer Care.

  • Page 29: 1: Unpack And Inspect The Reader

    1: Unpack and Inspect the Reader | 1: Unpack and Inspect the Reader The Synergy H1 with all available modules weighs up to  . Use two people when lifting and 55 pounds (24.95 kg) carrying the instrument. Save all packaging materials. If you need to ship the reader to BioTek for repair or replacement, you must use the original materials.

  • Page 30: 2: Unpack And Inspect The Dispense Module

    2: Unpack and Inspect the Dispense Module  Save all packaging materials. If you need to ship the dispense module to BioTek for repair or replacement, you must use the original materials. Using other forms of commercially available packaging, or failing to follow the repackaging instructions,...

  • Page 31: 3: Unpack And Inspect The Gas Controller

    3: Unpack and Inspect the Gas Controller  Save all packaging materials. If you need to ship the gas controller module to BioTek for repair or replacement, you must use the original materials. Using other forms of commercially available packaging, or failing to follow the...

  • Page 32: 5: Remove The Shipping Hardware

    If you are installing the BioStack for operation with the  Synergy H1, be sure to allow enough room for both instruments and the host computer. The BioStack and the reader require a space approximately 42" D x 17" W x 24" H (for 30-plate stacks) or 34"...

  • Page 33: 6: Install The Power Supply

    If you are not installing the gas controller module, place the dispense module on top of the reader.  Do not place the module next to the reader. Synergy H1 Operator’s Manual...
  • Page 34 Remove the tubing feed-through cover from the top of the reader (2 screws). Store the cover and screws with the shipping hardware in case the reader needs to be shipped again. BioTek Instruments, Inc.
  • Page 35  A magnet located between the injector tips helps to guide the tips into place and secures them in the reader. Place the light shield over the hole in the top of the reader and finger-tighten the thumbscrews to secure it. Synergy H1 Operator’s Manual...
  • Page 36 Carefully pull down the bottom of the syringe until it rests inside the hole • in the bracket. • Pass a thumbscrew up through this hole and thread it into the bottom of the syringe. Hold the syringe to prevent it from rotating while tightening the thumbscrew. Finger-tighten only. BioTek Instruments, Inc.

  • Page 37: 9: Connect The Host Computer

    9: Connect the Host Computer The Synergy H1 is equipped with two communication ports: Serial (RS-232) and USB. Both ports are located on the back of the reader. Two communication cables are included in the accessories box. Determine which •...

  • Page 38: 10: Install Gen5 On The Host Computer

    10: Install Gen5 on the Host Computer  The Synergy H1 is controlled by Gen5 software running on a host computer. There is a certain sequence of events that must be followed to ensure that the software is properly installed and configured.

  • Page 39: 13: Run A System Test

    13: Run a System Test | Select • Plug & Play A Synergy H1 must be connected to the computer and turned on to  appear in the Available Plug & Play Readers list. Click . Gen5 attempts to communicate with the reader. If the Test Comm communication attempt is successful, return to Gen5’s main screen.

  • Page 40: 14: Test The Injector System

    If the problem is something you can fix, do so now and run another System Test. If the problem is something you cannot fix, or if the test continues to fail, contact BioTek’s Technical Assistance Center. Turn off the incubator: •...
  • Page 41 Fill the two reagent bottles with distilled or deionized water. Place the bottles in their holders, and place the holders directly in front of the syringes. Insert the inlet tubes into the bottles. In Gen5, select System > Instrument Control > Synergy H1 Click the tab. Dispenser...

  • Page 42: Operational/Performance Qualification

    | Chapter 2: Installation Operational/Performance Qualification Your Synergy H1 was fully tested at BioTek prior to shipment and should operate properly following the successful completion of the installation and setup procedures described in this chapter. If you suspect that problems occurred during shipment, if you received the reader back...
  • Page 43 BioTek and order PN 8040015 if you have misplaced the carrier shipping bracket and/or the filter reader shipping bracket. If you need to ship the Synergy H1 and/or the dispense module to BioTek for service or repair, be sure to use the original packaging materials.
  • Page 44 | Chapter 2: Installation Figure 7: Repacking the Synergy H1 accessories Place the instrument in a large plastic bag. Place the instrument in the shipping box with foam corners, as shown in Figure 8 BioTek Instruments, Inc.
  • Page 45 Place the accessories box in the shipping box, then seal the shipping box with tape. When finished, write the RMA number on the outside of the box and ship the box to BioTek. Figure 8: Repacking the instrument and accessories box Synergy H1 Operator’s Manual...

  • Page 46: Preparing The Dispense Module For Shipment

    | Chapter 2: Installation Preparing the Dispense Module for Shipment If you have not already done so, contact BioTek’s Technical Assistance Center for an RMA (Return Materials Authorization) number and the shipping address before returning equipment for service. Decontaminate the module according to the instructions in .
  • Page 47 Slide the dispenser accessories box into the shipping box. Insert the top foam end cap. Close and seal the outer box with tape. Write the RMA number on the outside of the box. Ship the box to BioTek. Synergy H1 Operator’s Manual...
  • Page 48 | Chapter 2: Installation Figure 10: Packing the dispense module BioTek Instruments, Inc.

  • Page 49: Getting Started

    Chapter 3 Getting Started This chapter describes some of the Synergy H1’s external and internal components, and provides an introduction to using BioTek Gen5 software to control the instrument. Modular Design ............... 30 External Components ............31 Internal Components ............31 Filter Cube ..............

  • Page 50: Modular Design

    | Chapter 3: Getting Started Modular Design The Synergy H1 is a multi-mode microplate reader, with a design that allows you to initially purchase only the detection capabilities you need and then upgrade later as your requirements expand. Please contact BioTek Customer Care to learn more about your upgrade options.

  • Page 51: External Components

    Page Filter Cube The filter cube can contain excitation and emission filters, mirrors, and polarizing filters. Preconfigured cubes are available from BioTek, or you can change the filters and mirrors yourself. Injector System The syringes may require replacement over time.

  • Page 52: Filter Cube

    | Chapter 3: Getting Started Filter Cube The Synergy H1 is equipped with a filter cube that contains excitation and emission filters, mirrors, and, if required, polarizing filters. Each filter cube contains two filter sets, each of which contains one excitation filter, one mirror, and one emission filter.

  • Page 53: Injector System

    BioTek recommended option. Contact BioTek for more information on purchasing additional mirrors and filter cubes. The Synergy H1’s filter cube stores up to two mirrors. There are two possible mirror types: mirror is a glass slide with silver dots. It works with any •...
  • Page 54 Valves switch the syringe flow from the inlet tubes to the outlet tubes.  Outlet tubes transport fluid from the syringes into the instrument, through the tubing ports on the Synergy H1’s top cover. The outlet tubes are opaque PTFE tubes with threaded fittings on each end. BioTek Instruments, Inc.
  • Page 55 96 wells are too tall for error-free tip priming; and, tip priming is rarely required for these larger-volume plates.  The priming tray should be empty before priming and contain fluid after priming. Synergy H1 Operator’s Manual...

  • Page 56: Gen5 Software

    Figure 3: The priming trough and plate installed on the microplate carrier Gen5 Software BioTek Gen5 software supports all Synergy H1 reader models. Use Gen5 to control the reader and the dispense module (if equipped), perform data reduction and analysis on the measurement values, print or export results, and more.

  • Page 57: Protocols And Experiments

    Create a new experiment. Gen5 version 2.x users: From the Task Manager, select • Experiments > Create using an existing protocol • Gen5 version 1.x users: From the Welcome Screen, select Experiment Select the desired protocol and click Synergy H1 Operator’s Manual...

  • Page 58: Dispense Module Control

    Fill the supply bottle with a sufficient volume of the fluid to be used for the prime and the assay. Insert the appropriate inlet tube into the bottle. In Gen5, select and click System > Instrument Control > Synergy H1 tab. Dispenser Select the Dispenser number ( ) associated with the supply bottle.

  • Page 59: Recommendations For Optimum Performance

    Filter solutions to remove particulates that could cause erroneous readings. Although the Synergy H1 supports standard flat, U-bottom, and V-bottom microplates, • the reader achieves optimum performance with flat-bottomed wells when running in Absorbance mode.

  • Page 60: Kinetic Assays Using The Continuous Shake Feature

    (this example assumes the Read step takes one minute): Luminescence Measurements For highly sensitive Luminescence assays using white plates, add a Delay step to your Procedure to “dark adapt” the plates in the Synergy H1’s reading chamber before taking measurements. BioTek Instruments, Inc.

  • Page 61: Monochromator-Based Fluorescence Systems

    Cluster your sample wells rather than spacing them throughout the plate. Place your sample wells in the center of the plate. This placement may lead to less • evaporation than if you place the samples in wells on the edge of the plate. Synergy H1 Operator’s Manual...
  • Page 62 | Chapter 3: Getting Started BioTek Instruments, Inc.
  • Page 63 Filters and Mirrors provided an overview of the filters and Chapter 3, Getting Started mirrors installed in some Synergy H1 models. This chapter provides more detailed information on working with these components. Filter Cube Overview ............44 Removing a Filter Cube ............. 46 Installing a Filter Cube............

  • Page 64: Filters And Mirrors

    Figure 3 Figure 1: Filter cube You can easily exchange the Synergy H1’s filter cube to meet your assay requirements. If you regularly need to change the filters or mirrors on the reader, consider purchasing additional filter cubes from BioTek to make the process easier and faster.
  • Page 65 The filter cube can hold up to two half-size or one full-size dichroic or 50% mirror. The mirror positions are labeled “1”and “2” to coordinate with EX1/EM1 and EX2/EM2. For Synergy H1 models with FP capability, the cube is equipped with up to four polarizers of the following types: •...

  • Page 66: Removing A Filter Cube

    Ensure that all filters, plugs, and mirrors are inserted properly in the filter cube (see Figure 2 Figure 3 Open the front access door and locate the filter cube chamber ( Figure 4 filter cube chamber Figure 4: Locating the filter cube chamber BioTek Instruments, Inc.

  • Page 67: Adding A Filter Cube To The Optics Library

    Define the Filter Cube Getting Started Click System > Optics Library > Filter Cubes Click and enter a name for the filter cube. This is then available for selection in the protocol/experiment procedure. Synergy H1 Operator’s Manual...

  • Page 68: Filters And Mirrors

    580–655 665–850 Define Filter Set 2, if necessary. Click Filters and Mirrors Synergy H1 is equipped with filters for obtaining fluorescence excitation emission and luminescence measurements. The excitation filter selects the band of light to which the sample will be exposed. The emission filter selects the band of light with the maximum fluorescence signal of the sample, to be measured by the photomultiplier tube (PMT).

  • Page 69: Change The Filters And Mirrors

    Filters and mirrors are stored in the filter cube; see page 44 for an overview. You can replace the entire filter cube with a different one; this is the BioTek-recommended option. Alternatively, you can install different filters or mirrors in the cube. Contact BioTek for more information on purchasing additional filters and mirrors.
  • Page 70 The bottom of the cube contains the excitation filters and mirrors. Remove the mirrors before removing the excitation filters: a. Make note of the mirror placement and label orientation. BioTek Instruments, Inc.
  • Page 71 Place the filter cube top back into the cube and replace the screw and washer. When finished, install the filter cube in the reader. Synergy H1 Operator’s Manual...

  • Page 72: Clean The Filters And Mirrors

    Preventive Maintenance Filters Available from BioTek Bandpass filters are available for purchase from BioTek. See the list at www.biotek.com/products/accessories. Please note that part numbers are subject to change, and new filters may become available. Contact BioTek Customer Care with any questions.
  • Page 73 Chapter 5 Preventive Maintenance This chapter provides instructions for maintaining the Synergy H1 and external dispense module (if used) in top condition, to ensure that they continue to perform to specification. Preventive Maintenance ............ 54 Daily Cleaning for the Dispense Module ......54 Schedule ..............

  • Page 74: Preventive Maintenance

    | Chapter 5: Preventive Maintenance Preventive Maintenance A general Preventive Maintenance regimen for all Synergy H1 models includes periodically cleaning all exposed surfaces and inspecting/cleaning the emission and excitation filters and mirrors (if used). For models with the external dispense module, additional tasks include flushing/purging the fluid path, and cleaning the tip prime trough, priming plate, supply bottles, dispense tubing, and injectors.

  • Page 75: Warnings And Precautions

    Do not immerse the instrument, spray it with Important! liquid, or use a “wet” cloth on it. Do not allow water or other cleaning solution to run into the interior of the instrument. If this happens, contact BioTek’s Technical Assistance Center. Synergy H1 Operator’s Manual...

  • Page 76: Clean Exposed Surfaces

    Deionized or distilled water • • Clean, lint-free cotton cloths • Mild detergent (optional) Turn off and unplug the instrument. Moisten a clean cotton cloth with water, or with water and mild detergent. Do not soak the cloth. BioTek Instruments, Inc.

  • Page 77: Inspect/Clean Excitation And Emission Filters

    The internal chamber and probes are not customer-accessible. If overflow is significant, contact BioTek’s Technical Assistance Center with any questions about your particular model. Inspect/Clean Excitation and Emission Filters Laboratory air is used to cool the lamp, and the filters can become dusty as a result.

  • Page 78: Inspect/Clean Mirrors

    • 100% pure cotton balls (for the polarizing filters) • Procedure Turn off and unplug the reader. Open the access door on the front of the instrument and slide the filter cube straight out of its compartment. BioTek Instruments, Inc.
  • Page 79 Kimwipe. To replace the mirror, hold it by its edges, turn it so that its label is face-up and readable, and place it on the shelf in the filter cube. See Figure 2 Synergy H1 Operator’s Manual...

  • Page 80: Flush/Purge The Fluid Path

    Fill two supply bottles with deionized or distilled water. Insert the supply (inlet) tubes into the bottles. Place the priming plate on the carrier. Select System > Instrument Control > Synergy H1 Click the tab and select Dispenser Dispenser 1.

  • Page 81: Run A Dispense Protocol (Optional)

    To purge the fluid from the system: Place the inlet tubes in empty supply bottles or a beaker. Select System > Instrument Control > Synergy H1 Click the tab and select Dispenser Dispenser 1 Set the Volume to 2000 µL...

  • Page 82: Empty/Clean The Tip Priming Trough

    Applies only to models with injectors. Synergy H1 The tip priming trough is a removable cup located in the rear pocket of the microplate carrier, used for performing the Tip Prime. The trough holds about 1.5 mL of liquid and must be periodically emptied and cleaned by the user.

  • Page 83: Clean The Dispense Tubes And Injectors

    Clean the Dispense Tubes and Injectors Applies only to Synergy H1 models with injectors. The Synergy H1’s dispense tubes and injectors require routine cleaning, at least quarterly and possibly more frequently depending on the type of fluids dispensed. Required Materials Protective gloves •...

  • Page 84: Clean The Dispense Tubes And Injectors

    Stream water through the pipe to be sure it is clean. If the water does not stream out, try soaking in hot, soapy water and then reinserting the stylus.  Be careful not to bend the injector tips. A bent tip might not dispense accurately. BioTek Instruments, Inc.

  • Page 85: As-Needed Maintenance

    Chapter 6 As-Needed Maintenance This chapter contains maintenance and component-replacement procedures that need to be performed only occasionally. Decontamination ............. 66 Required Materials ............67 Procedure for Models without the Dispense Module ..67 Procedure for Models with the Dispense Module ....68 Routine Procedure...........

  • Page 86: Decontamination

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazard(s) they handle.

  • Page 87: Decontamination

    Decontamination | Required Materials For all Synergy H1 models: • Sodium hypochlorite (NaClO, or bleach) 70% isopropyl alcohol (as an alternative to bleach) • Deionized or distilled water • Safety glasses • • Surgical mask • Protective gloves Lab coat •...

  • Page 88: Procedure For Models With The Dispense Module

    Procedure for Models with the Dispense Module  Perform the Routine Procedure when the Synergy H1 is functioning normally. If you are unable to perform a prime due to a system failure, perform the Alternate Procedure described on page 71.

  • Page 89: Decontaminate The Fluid Lines

    Empty the beaker containing the outlet tubes. Put the tubes back in the empty beaker. If sodium hypochlorite (bleach) was used, perform Rinse the Fluid Lines Otherwise (or after performing the Rinse procedure), repeat steps 1–13 for SYRINGE 2/Dispenser 2. Synergy H1 Operator’s Manual...

  • Page 90: Rinse The Fluid Lines

    If decontaminating in a bleach solution, thoroughly rinse the trough and • plate with DI water. If decontaminating with alcohol, let the trough and plate air dry. • Discard the used gloves and cloths using a biohazard trash bag and an approved biohazard container. BioTek Instruments, Inc.

  • Page 91: Alternate Procedure

    Decontamination | Alternate Procedure If you are unable to prime the Synergy H1 due to a system failure, decontaminate the instrument and the dispense module as follows: Perform the procedures under Clean the Tubing and Injectors Chapter 5, Preventive Maintenance.

  • Page 92: Dispense Module: Syringe Replacement

    Accuracy and Precision tests fail). If cleaning the dispense module does not eliminate performance problems, or if a syringe is obviously leaking, perform these instructions to replace a faulty syringe. Contact BioTek TAC to order replacement syringes. To change a syringe, first use Gen5 to put the syringe in its maintenance position.
  • Page 93 Hold the syringe from rotating while tightening the thumbscrew. Finger-tighten only. In Gen5, select . Click the System > Instrument Control > Synergy H1 tab and click Dispenser Initialize Synergy H1 Operator’s Manual...
  • Page 94 Chapter 6: As-Needed Maintenance BioTek Instruments, Inc.

  • Page 95: I Nstrument Qualification

    Chapter 7 I nstrument Qualification This chapter contains procedures for qualifying the initial and ongoing performance of the Synergy H1 and the external dispense module (if equipped). Overview ................ 77 IQ/OQ/PQ ............... 77 Recommended Qualification Schedule ......... 78 System Test ..............79 Absorbance Plate Test ............
  • Page 96 | Chapter 7: Instrument Qualification Troubleshooting ............128 Dispense Module Tests ............ 129 Required Materials ............130 Alternate Test Solutions ..........130 BioTek Instruments, Inc.

  • Page 97: Overview

    Operational Qualification (OQ), and Performance Qualification (PQ) procedures for all models of the Synergy H1 Multi-Mode Microplate Reader. Every Synergy H1 reader and external dispense module is fully tested at BioTek prior to shipment and should operate properly upon initial setup. If you suspect that a problem...

  • Page 98: Recommended Qualification Schedule

    Recommended Qualification Schedule The schedule below defines BioTek-recommended intervals for qualifying a Synergy H1 used two to five days a week. The actual frequency, however, may be adjusted depending on your usage of the instrument and its various modules. This schedule assumes the reader is properly maintained as outlined in the chapter.

  • Page 99: System Test

    Absorbance Test Plate. System Test Each time the Synergy H1 is turned on, it automatically performs a series of tests on the reader’s motors, lamp, the PMT, and various subsystems. The duration of this System Test depends on the reader model, and can take a few minutes to complete.
  • Page 100 If the test continues to fail, or if the cause is not something you can fix, contact BioTek’s Technical Assistance Center.  A sample test report for a Synergy H1 model with all modules installed is shown on the next few pages. Your instrument’s test report may be different.
  • Page 101 10667 Delta 2138 29250 #5:800 2.85 Light 12682 39791 Dark 10693 10689 Delta 1989 29102 #6:999 1.99 Light 12571 39868 Dark 10672 10677 Delta 1899 29191 Noise Test Meas Gain 1.00 10646 10653 10645 10653 Delta Synergy H1 Operator’s Manual...
  • Page 102 18995 25037 27778 16065 19212 25386 27974 15413 18859 24708 27471 StdDev #3:425 Light 32558 26820 43606 36203 Dark 10713 1708 10713 1705 Delta 21845 25112 32893 34498 22258 25436 33493 34737 21385 24819 32546 34192 StdDev BioTek Instruments, Inc.
  • Page 103 10713 1710 10713 1707 Delta 17292 11743 25788 16019 17601 11841 26090 16148 16936 11619 25383 15936 StdDev #6:700 Light 16817 2526 19895 2851 Dark 10713 1709 10713 1708 Delta 6104 9182 1143 6187 9282 1158 Synergy H1 Operator’s Manual...
  • Page 104 6656 - 6660= Delta 4 448 - 448= Carrier - Injectors Upper Left x= 2200 y= 6636 Lower Left x= 2196 Lower Right x=11968 Upper Right x=11972 y= 6640 Delta 1 2200 - 2196= Delta 2 11972 -11968= BioTek Instruments, Inc.

  • Page 105: Absorbance Plate Test

    T his test uses BioTek’s Absorbance Test Plate (PN 7260522) to confirm the mechanical 1 0 B alignment; optical density accuracy, linearity, and repeatability; and wavelength accuracy of the Synergy H1. The Absorbance Plate Test compares the reader’s optical density and wavelength measurements to NIST-traceable values. Synergy H1 Operator’s Manual...

  • Page 106: Define Absorbance Test Plate Parameters

    | Chapter 7: Instrument Qualification  An alternate method for confirming accuracy, linearity, and repeatability is Liquid Test 2, described on page 94. To run this test, you will need BioTek’s Absorbance Test Plate (PN 7260522), with its accompanying data sheet. •...

  • Page 107: Run The Absorbance Plate Test

    Wavelength Test Highlight the wavelength(s) to be included in this test. You need to select only those wavelengths most appropriate for your use of the reader. (Optional) Enter any Comments. Click Start Test Synergy H1 Operator’s Manual...

  • Page 108: Sample Absorbance Plate Test Report

    We recommend you print and save the report to document that the test was performed. Sample Absorbance Plate Test Report Absorbance Test Plate Results Reader: Synergy H1 (Serial Number: 122961) Basecode: P/N 8040200 (v1.00.0) Date and Time:...
  • Page 109 0.599 1.113 1.692 1.820 2.416 Read 2 0.171 0.588 1.097 1.671 1.797 2.340 Result PASS PASS PASS PASS PASS PASS Reviewed/Approved By: _________________________________ Date: ________________ Figure 2: Sample output for the Synergy H1 Absorbance Plate Test. Synergy H1 Operator’s Manual...

  • Page 110: Results And Troubleshooting Tips

     Make sure the Test Plate is within its calibration certification period. The calibration sticker is affixed directly to the plate. If it is out of date, contact BioTek to schedule a recertification.  Check the microplate carrier to ensure it is clear of debris.

  • Page 111: Absorbance Liquid Tests

    Stock Solution A or B, which may be formulated by diluting a dye solution available from BioTek (A) or from the ingredients listed below (B). Solution A • BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; PN 7120782, 125 mL) • Deionized water 5-mL Class A volumetric pipette •...
  • Page 112 Weigh out 0.092 g of FD&C Yellow No. 5 dye powder into a weigh boat. Rinse the contents into a 1-liter volumetric flask. Add 0.5 mL of Tween 20, or 5 mL of BioTek’s wetting agent. Fill to 1 liter with DI water; cap and shake well. The solution should measure approximately 2.000 OD when using 200 µL in a flat-bottom...
  • Page 113 ± 1.0% ± 0.005 OD from 0.000 to 2.000 OD ± 3.0% ± 0.005 OD from 2.000 OD to 2.500 OD Accuracy Specification: ± 1.0% ± 0.010 OD from 0.000 to 2.000 OD ± 3.0% ± 0.010 OD from 2.000 OD to 2.500 OD Synergy H1 Operator’s Manual...

  • Page 114: Absorbance Liquid Test 2

    80% in the third tube, all the way to 10% in the tenth tube. Dilute using the 0.05% solution of deionized water and Tween 20. This solution can also be made by diluting the BioTek wetting agent 200:1. Tube Number:...
  • Page 115 Using the plate prepared for the Linearity Test on the previous page, conduct a Turnaround test by reading the plate with the A1 five times well in the H12 position. Save the data after each read. Synergy H1 Operator’s Manual...

  • Page 116: Absorbance Liquid Test 3 (Optional)

    Calibrated hand pipette(s) • • Beakers and graduated cylinder • Precision balance with readability to 0.01 g Buffer solution described below • Buffer Solution • Deionized water • Phosphate-Buffered Saline (PBS), pH 7.2–7.6, Sigma tablets, #P4417 (or equivalent) BioTek Instruments, Inc.
  • Page 117 β-NADH Powder (β-Nicotinamide Adenine Dinucleotide, Reduced Form) • Sigma bulk catalog number N 8129, or preweighed 10-mg vials, Sigma number N6785-10VL (or BioTek PN 98233). Store the powder according to the guidelines on its packaging. Prepare a PBS solution from the Sigma tablets.
  • Page 118 R Square value of at least 0.99 is considered adequate. Repeatability Specification: ± 1.0% ± 0.005 OD from 0.000 to 2.000 OD ± 3.0% ± 0.005 OD from 2.000 OD to 2.500 OD BioTek Instruments, Inc.

  • Page 119: Fluorescence Liquid Tests

    Verifies the performance of the xenon flash bulb and that • (Optional) TRF Test: the filters, optical path, and PMT are all in working order. BioTek offers a pre- configured qualification TRF filter cube for purchase*; contact BioTek Customer Care and ask about part number 8030555.

  • Page 120: Required Materials

    Gen5 protocols listed in the next table and described starting on page 113 For the Filter-Based Fluorescence System: H1_FI_T_SF.prt Corners, Sensitivity, and Linearity tests, using the Top optics H1_FI_T_MUB.prt Alternative top optics test, using methylumbelliferone H1_FP.prt Fluorescence Polarization test H1_TRF.prt Time-Resolved Fluorescence test BioTek Instruments, Inc.
  • Page 121 H1_M_FI_T_MUB.prt Alternative top optics test, using methylumbelliferone Filter Set Setup Before using the filter-based fluorescence test protocols, create the applicable filter sets shown below in Gen5 (“Green” is used for sodium fluorescein tests; “Blue” for MUB). Synergy H1 Operator’s Manual...
  • Page 122 | Chapter 7: Instrument Qualification Corners/Sensitivity/Linearity Tests  BioTek offers liquid test kit PN 7160010 containing the microplates and solutions used in all (SF/MUB/Eu) fluorescence liquid test procedures. Kits for each individual procedure are also available; see the Optional Accessories section in the Introduction chapter. Sodium Fluorescein Test Kit PN 7160013 contains the buffer and SF already pre-diluted.

  • Page 123: Test Solutions

    • • 510-nm dichroic mirror and polarizers installed Time-Resolved Fluorescence (TRF) Test  BioTek offers a pre-configured qualification TRF filter cube for purchase; see page 99. • 15-mL conical-bottom, polypropylene sample tube Excitation filter 360/40 nm and emission filter 620/40 nm installed •...
  • Page 124 Fluorescence Polarization (FP) Test As described on page 102 , the recommended test solutions are available from Invitrogen Corporation or BioTek. They do not require additional preparation. Time-Resolved Fluorescence (TRF) Test As described on page 103 , the recommended test solutions are available from Invitrogen Corporation or BioTek.

  • Page 125: Procedure

    Pipette the solutions for the “TRF” test into a new 96-well solid white • plate. • Create an experiment based on the protocol. Read the H1_TRF.prt plate and then save the experiment. Calculate and evaluate results as described under Results Analysis starting on page 109. Synergy H1 Operator’s Manual...

  • Page 126: Pipette Maps

    Aspirate 150 µL from column 2 and dispense into column 3. Mix the wells • using the pipette. Do not discard the tips. • Aspirate 150 µL from column 3 and dispense into column 4. Mix the wells using the pipette. Do not discard the tips. BioTek Instruments, Inc.
  • Page 127 SF solutions and buffer. This will dilute the wells as shown in the following map: 0.75 0.375 0.1875 0.0938 0.0469 0.75 0.375 0.1875 0.0938 0.0469 Sensitivity/ Linearity: 0.75 0.375 0.1875 0.0938 0.0469 0.75 0.375 0.1875 0.0938 0.0469 Synergy H1 Operator’s Manual...
  • Page 128 If you have not already done so, shake the vial of 20 pM europium suspension • vigorously for 30 seconds prior to pipetting. Alternatively, sonicate or vortex the vial. Pipette 200 µL of the 20 pM europium suspension (Eu) into wells A8–B8. • BioTek Instruments, Inc.

  • Page 129: Results Analysis

    – 750 / ((Mean SF Mean Buffer)/(3 * Standard Deviation Buffer)) To pass, the Detection Limit must be Optic Probe less than or equal to: EX 485 nm, EM 528 nm Top/Bottom: 20 pM (3.76 pg/mL) Synergy H1 Operator’s Manual...
  • Page 130 Calculate the Signal for each LPR well: Subtract the Mean Blank from its • measurement value. Using the raw data from the Perpendicular red: • Calculate the Mean Blank (wells A6–H6) Calculate the Signal for each HPR well: Subtract the Mean Blank from • its measurement value. BioTek Instruments, Inc.
  • Page 131 20000 / ((Mean Eu – Mean DI water) / (3 * Standard Deviation DI water) To pass, the Detection Limit Optic Probe must be less than or equal to: Top, with 400 nm 250 fM dichroic mirror Synergy H1 Operator’s Manual...

  • Page 132: Troubleshooting

    • • For injector models, spilled fluid inside the reader may be fluorescing, which can corrupt your test results. If you suspect this is a problem, contact BioTek TAC. When testing Fluorescence Polarization capability using a solid black plastic •...

  • Page 133: Gen5 Protocol Reading Parameters

    1 (filter cube) Filters: EX 485/20 nm, EM 528/20 nm Optics Position: Top 510 nm Gain/Sensitivity: Corners Read: Auto, Scale to High Wells, A1, 50000 Sensitivity/Linearity Read: Auto, Scale to High Wells, C1, 50000 Read Height: 7.00 mm Synergy H1 Operator’s Manual...
  • Page 134 Delay After Plate Movement: 350 msec Measurements Per Data Point: Lamp Energy: Low (faster) Time Resolved: Enabled Delay Before Collecting Data: 300 µsec Data Collection Time: 1000 µsec Filter Sets: 1 (filter cube) Filters: EX 360/40 nm, EM 620/40 nm BioTek Instruments, Inc.

  • Page 135: Fluorescence Test Procedure (Methylumbelliferone)

     Microplates should be perfectly clean and free from dust or bottom scratches. Use new microplates from sealed packages.  Manufacturer part numbers are subject to change. Methylumbelliferone (“MUB”) (10-mg vial, BioTek PN 98156) • • Carbonate-Bicarbonate buffer (“CBB”) capsules (Sigma #3041) •...
  • Page 136 Open and dissolve the contents of two CBB capsules (do not dissolve • the outer gelatin capsule) into 200 mL of the water. Stir the solution (preferably using a stir table) until the CBB is • completely dissolved. BioTek Instruments, Inc.
  • Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. Create an experiment based on the protocol. • H1_M_FI_T_MUB.prt Read the plate and then save the experiment. Calculate and evaluate the results as described under Results Analysis page 119. Synergy H1 Operator’s Manual...
  • Page 138 If your model is equipped with the monochromator-based fluorescence system, after testing the top optics of the filter-based system, you will pipette 50 µL of buffer on top of the existing MUB solutions and buffer. This will dilute the wells as shown next. BioTek Instruments, Inc.
  • Page 139 Calculate the Mean for the 13.2 ng/mL (75 nM) MUB solution wells (C1–F1). Calculate the Detection Limit, in ng/mL: 13.2 / ((Mean MUB – Mean Buffer)/(3 * Standard Deviation Buffer)) To pass, the Detection Limit Optic Probe must be less than or equal to: 0.16 ng/mL Synergy H1 Operator’s Manual...
  • Page 140 Measurements Per Data Point: Read Wells: Wells C1–F12 Lamp Energy: Low (faster) Filter Sets: Filters: EX 360/40 nm, EM 460/40 nm Optics Position: Top 400 nm Gain/Sensitivity: Auto, Scale to High Wells, C1, 50000 Read Height: 7.00 mm BioTek Instruments, Inc.
  • Page 141 Normal Delay After Plate Movement: 350 msec Measurements Per Data Point: Lamp Energy: Low (faster) Wavelengths 1, EX 360 nm, EM 460 nm Optics: Gain/Sensitivity: Auto, Scale to High Wells, C1, 50000 Read Height: 7.00 mm Synergy H1 Operator’s Manual...

  • Page 142: Luminescence Test

    Luminescence Test Applies only to models with Luminescence capabilities. For Synergy H1 models with luminescence capability, BioTek provides two methods for verifying the performance of the luminescence read. One method measures a Harta Luminometer Reference Microplate, which is an LED-based test plate. Contact BioTek to purchase a plate, or go to www.hartainstruments.com for more information.
  • Page 143 If the reader is equipped with the red-shifted PMT, the detection limit must • to pass. <= 500 amol  To determine which PMT is installed, check the label on the back of the reader. #49984 = low-noise PMT; #49721 = red-shifted PMT Synergy H1 Operator’s Manual...

  • Page 144: Glowell Test

    Glowell Test Materials • Glowell, PN GLO-466, formerly available from LUX BioTechology, Ltd. (www.luxbiotech.com) Glowell Adapter Plate, available from BioTek, PN 7160006 • Gen5 protocol(s) (see page 128 • Procedure If you have not already done so, insert the Glowell (“window” side up) into well D8 of the Adapter Plate.

  • Page 145: Gen5 Protocol Reading Parameters

    The information in the following tables represents the recommended reading parameters. F-LumTest_Harta.prt Parameter Default Setting Plate Type: “Costar 96 black opaque” (#3915) Delay Step: 3 minutes Read Step 1: Read Wells: Label: Reference well A2 Detection Method: Luminescence Read Type: Endpoint Synergy H1 Operator’s Manual...
  • Page 146 Battery Check Detection Method: Luminescence Read Type: Endpoint Integration Time: 0:01.00 MM:SS.ss Delay After Plate Movement: 350 msec Dynamic Range: Standard Filter Sets 1 (filter cube) Excitation Plug Emission: Hole Optics Position: None Gain/Sensitivity: Read Height: 10.00 mm BioTek Instruments, Inc.
  • Page 147 Read Height: 1.00 mm Read Step 3: Read Wells: A7–A8 Label: Battery Check Detection Method: Luminescence Read Type: Endpoint Integration Time: 0:01.00 MM:SS.ss Delay After Plate Movement: 350 msec Dynamic Range: Standard Gain/Sensitivity: Read Height: 1.00 mm Synergy H1 Operator’s Manual...

  • Page 148: Troubleshooting

    1.00 mm Troubleshooting If either test fails, try the following suggestions. If the test(s) continues to fail, print the results and contact BioTek’s Technical Assistance Center. Ensure that the reading is performed through a hole in the filter cube, not •...

  • Page 149: Dispense Module Tests

    Dispense Module Tests Applies only to models with the Dispense module. BioTek Instruments, Inc., has developed a set of tests that you can perform to ensure that the dispense module performs to specification initially and over time. We recommend that you perform these tests before first use (e.g., during the Initial OQ), and then every three...

  • Page 150: Required Materials

    1.300 and 1.700 OD at 405/750 nm. The solutions should be at room temperature. If you do not have BioTek’s Green Test Dye Solution (PN 7773003), prepare a dye solution using one of the following methods: BioTek Instruments, Inc.
  • Page 151 | 131 Dispense Module Tests Using BioTek’s Blue and Yellow Concentrate Dye Solutions: Ingredient Quantity Concentrate Blue Dye Solution (PN 7773001, 125 mL) 4.0 mL QC (Yellow) Solution (PN 7120782, 125 mL) 5.0 mL Deionized water 90.0 mL Using FD&C Blue and Yellow Dye Powder:...
  • Page 152 Procedure for Models without Absorbance Capabilities  If you will not be using a BioTek absorbance reader for this procedure, prepare your reader to perform two reads with the following characteristics: 80 µL Read...
  • Page 153 • Close the experiment without saving it.  If you are not using a BioTek reader for taking the absorbance measurements, read the plate using the wavelengths shown in the table on the previous page, and then perform the Results Analysis as described on page 134.
  • Page 154 • The injectors may require cleaning (see Preventive Maintenance Each dispense module is factory-calibrated for the Synergy H1 it ships with. • Verify that the serial number on the dispense module matches the serial number on the reader. Even if the serial numbers match, it is still possible that the calibration values have been inadvertently changed.
  • Page 155  Perform these steps to create a protocol to test Dispenser 1. Then, open a copy of the protocol and change the relevant Procedure parameters for Dispenser 2. In Gen5, create a new Synergy H1 protocol. Define the with the steps and settings as described in this table:...
  • Page 156 A5 to H8 Disp_20  A9 to H12 Disp_5  After running the experiment, view the Statistics for each Delta OD  Data Set to view the calculations. Save the protocols as Disp 1 Test.prt Disp 2 Test.prt BioTek Instruments, Inc.
  • Page 157 The procedure is performed twice, once for each dispenser. You will create two Gen5 protocols to perform the dispense steps. If you will use a BioTek absorbance reader that is supported by Gen5, you will create one additional protocol to perform the Read steps.
  • Page 158 | Chapter 7: Instrument Qualification Create the Read Protocol (if needed) In Gen5, create a new protocol for the BioTek reader. Define the with the steps and settings as described in this table: Procedure Step Type Details Shake Medium intensity for 15 seconds...
  • Page 159 Assign Disp_20 to wells A5 to H8. • Assign Disp_5 to wells A9 to H12. After running the experiment, view the Statistics for each Delta OD  Data Set to view the calculations. • Save the protocol as Disp Test Other Reader.prt Synergy H1 Operator’s Manual...
  • Page 160 | Chapter 7: Instrument Qualification BioTek Instruments, Inc.

  • Page 163: Specifications

    Appendix A Specifications This appendix contains BioTek’s published specifications for the Synergy H1. General Specifications ............142 Absorbance Specifications ............ 144 Dispense/Read Specifications ..........145 Fluorescence Specifications (Mono-Based) ......145 Fluorescence Specifications (Filter-Based) ....... 146 Luminescence Specifications ..........147...

  • Page 164: General Specifications

    | Appendix A: Specifications General Specifications Microplates The Synergy H1 accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry and the Take3 and Take3Trio Micro-Volume Plates. Maximum Plate Height: Absorbance mode: plates up to 0.8" (20.30 mm) high Fluorescence, monochromator-based mode: plates up to 0.89"...

  • Page 165: Dispense/Read Specifications

    Double Orbital Slow: Amplitude: 1 mm to 6 mm in 1-mm steps Frequency: ~10 Hz to ~3 Hz Double Orbital Fast: Amplitude: 1 mm to 6 mm in 1-mm steps Frequency: ~14 Hz to ~5 Hz Synergy H1 Operator’s Manual...

  • Page 166: Absorbance Specifications

    96-well and 384-well plate, normal read speed 0–2 OD: ±1% ±0.005 OD, delay after plate movement=100 ms 2–2.5 OD: ±3% ±0.005 OD, delay after plate movement=100 ms 96-well and 384-well plate, sweep read speed 0–1 OD: ±2% ±0.010 OD BioTek Instruments, Inc.

  • Page 167: Dispense/Read Specifications

    ≤10.0% for volumes of 5–9 µL Fluorescence Specifications (Mono-Based) The Synergy H1 measures fluorescence with monochromators from the top and bottom of 6- to 384-well plates. The following requirements apply to 96-well plates, with 200 µL/well, at room temperature. The Detection Limit is calculated as 3 * (Standard Deviation of 8 blank wells) / Slope of the concentration curve.

  • Page 168: Fluorescence Specifications (Filter-Based)

    | Appendix A: Specifications Fluorescence Specifications (Filter-Based) The Synergy H1 measures fluorescence with filters from the top of 6- to 384-well plates. The following requirements apply to 96-well plates, with 200 µL/well, at room temperature. The Detection Limit is calculated as 3 * (Standard Deviation of 8 blank wells) / Slope of the concentration curve.

  • Page 169: Luminescence Specifications

    Luminescence Specifications | Luminescence Specifications The Synergy H1 measures luminescence from the top of 6- to 384-well plates. The following requirements apply to 96-well plates, with 200 µL/well, at room temperature. Production testing is done using a Lux BioTechnology Glowell which is a Gaseous Tritium Light Source.
  • Page 170 | Appendix A: Specifications BioTek Instruments, Inc.

  • Page 171: Error Codes

    Appendix B Error Codes This appendix lists and describes Synergy H1 error codes that may appear in Gen5. Overview ................150 Contact Info: BioTek Service/TAC ........150 Error Codes ................ 151...

  • Page 172: Overview

    Appendix B: Error Codes Overview When a problem occurs during operation with the Synergy H1, an error code appears in Gen5. Error codes typically contain four characters, such as “4168,” and in most cases are accompanied by descriptive text, such as “PMT overload error.” With many errors, the instrument will beep repeatedly;...

  • Page 173: Error Codes

    If an error code appears in Gen5, look for it here. If you find the code, follow the suggestions provided for solving the problem. If you cannot find the code or if you are unable to solve the problem, please contact BioTek’s Technical Assistance Center. The Gen5 Help system also provides troubleshooting tips.
  • Page 174 Try lowering the gain/sensitivity in your Read step(s). For models with the dispense module, the internal chamber may require cleaning (contact BioTek TAC). If a 4E18 error is detected during monochromator-based fluorescence, the luminescence probe may be picking up stray light. Try installing a plug in the filter cube.
  • Page 175 “home all axis” not performed.) This error can also occur if the carrier is inside and the newly-defined plate height is different from the most-recently specified plate height. To resolve this error, eject the carrier prior to running the experiment. Synergy H1 Operator’s Manual...
  • Page 176 Appendix B: Error Codes BioTek Instruments, Inc.

  • Page 177: Instrument Dimensions For Robotic Interface

    Robotic Interface This section shows the location of the microplate carrier in reference to the exterior surfaces of the Synergy H1 and the mounting holes on the bottom. Use the illustrations to facilitate system setup with a robotic instrument, such as the BioStack...
  • Page 178 | Appendix C: Instrument Dimensions for Robotic Interface Top View Bottom View The arrows point to special mounting holes for alignment caps for operation with the BioStack; note that the model shown is not gas-ready. BioTek Instruments, Inc.
  • Page 179 Appendix C: Instrument Dimensions for Robotic Interface | Left Side View Special alignment hardware is included in the BioStack’s alignment BioStack users: kit for correct positioning with the Synergy H1. Refer to the chapter in the Installation BioStack Operator’s Manual for instructions.
  • Page 180 | Appendix C: Instrument Dimensions for Robotic Interface BioTek Instruments, Inc.
  • Page 181 129 Applications Support, 6 overview, 129 biohazards, xi precision, 129 BioStack dispense tubes using with Synergy H1, 12, 155 cleaning, 63 carrier eject button, 18 removing, 63 changing a filter cube, 46 electrical grounding, xi changing filters, 49 emission filters...
  • Page 182 7 filter set setup, 101 connecting host computer, 17 luminescence, 122 dispense module, 13 filters establish communications, 18 available from BioTek, 52 gas controller module, 13 changing, 49 installing Gen5, 18 cleaning, 57 power supply, 13 removing, 49 selecting a location, 11...
  • Page 183 Index overview, 54 hardware, 142 priming plate luminescence, 147 cleaning, 62 microplates, 142 priming the injector system, 35 mono-based fluorescence, 145 product qualification package, 77 spilled fluids, xi product registration, x Support protocol parameters Applications, 6 fluorescence, 113, 120 syringe luminescence, 125 replacing, 72 protocols, 37...
  • Page 184 Index BioTek Instruments, Inc.

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