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Summary of Contents for Biotek ELx808
- Page 1 Absorbance Microplate Reader ELx808 ™ Operator’s Manual...
- Page 2 ...
- Page 3 ELx808™ Absorbance Microplate Reader, All Models Operator’s Manual April 2013 © 2013 Part Number 7341000 Revision P ® BioTek Instruments, Inc.
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Page 4: All Rights Reserved
BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers. -
Page 5: Table Of Contents
User Safety ................. xviii Safety Symbols ................xix Chapter 1: Introduction ............... 1 Introducing the ELx808 Absorbance Microplate Reader ......2 Reader Variations ............... 2 Hardware Features ................2 Software Features ................3 Package Contents ................3 Optional Accessories ................ 3 Specifications ................. - Page 6 Attach the Cable ............... 21 Install Gen5 Software on the Host Computer ......... 21 Establish Communication ............22 Changing the Baud Rate on the ELx808 ........22 11: Verify Performance ..............23 Before Repackaging the Instrument ..........24 Chapter 3: Operation ................. 25 Getting Started with Gen5 ...............
- Page 7 Validation Formula Examples ..........53 Formula Type ..............53 Validation Type Selection ............55 Formula Entry ..............55 Number of Required Controls/Blanks ........57 Cutoff Formulas ..............58 Greyzone Entry ..............59 Positive/Negative Calls for Cutoff ..........59 ELx808 Operator’s Manual...
- Page 8 System Test ................89 Checksum Test ................. 89 Absorbance Plate Test ..............89 Define the Test Plate Parameters ........... 89 Run the Plate Test ............... 90 Empty Carrier Test .............. 91 Liquid Tests ................92 Prepare Test Solutions ............92 BioTek Instruments, Inc.
- Page 9 Terms Used in the Error Codes Tables ........116 Error Codes (General) ..............117 Error Codes (Fatal) ............... 121 Appendix A: Sample Reports ............123 Appendix B: Instructions for Programming a New Assay ....131 Appendix C: Adjusting the Power Input Voltage Setting ....141 ELx808 Operator’s Manual...
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Page 10: Contact Information
802-655-4740 (outside the U.S.) Fax: 802-654-0638 E-Mail: tac@biotek.com European Coordination Center/Authorized European Representative ® BioTek Instruments GmbH Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany Internet: www.biotek.de Phone: +49 (0) 7136 9680 Fax: +49 (0) 7136 968 111 E-Mail: info@biotek.de BioTek Instruments, Inc. -
Page 11: Revision History
A). Added KC4 startup information to Appendix B. Added new Appendix E with two examples of assay kit instructions and directions for programming an assay. General: Edited and formatted text. Modified appearance of display screens. Standardized the presentation of significant digits. Changed “Abs” to “OD” throughout. ELx808 Operator’s Manual... - Page 12 Appendix B: Added new section: ‘Controlling the Reader with Gen5’. 3/08 General: Applied the BioTek template to the manual. Changed “Bio-Tek” to “BIOTEK” in startup screens to reflect this change in the reader’s basecode software. Preface: Added Microsoft®, Windows®, Windows XP, Windows 2000, Windows Vista™, and Excel®...
- Page 13 2.500 OD. It does not indicate a PASS or FAIL above 2.500 OD. 4/13 Preface: Added EN 61010-2-081 and EN 61010-2-010 to Directive 2006/95/EC Low Voltage (Safety) and EN 61010-2-101 to Directive 98/79/EC In Vitro Diagnostics. ELx808 Operator’s Manual...
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Page 14: Document Conventions
Intended Use Statement • The ELx808 is an absorbance microplate reader. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. The user must evaluate this instrument and (if used) PC-based software in conjunction with their specific assay(s). -
Page 15: Warranty & Product Registration
Warranty & Product Registration Take a moment to review the warranty information that shipped with your product. Please also register your product with BioTek to ensure that you receive important information and updates about the product(s) you have purchased. You can register online through the Customer Resource Center (CRC) at www.biotek.com or by calling 888-451-... -
Page 16: Hazards
Warning! Hot Surface . The lamp is hot when the instrument is turned on. Turn off the reader and allow the lamp to cool for at least 15 minutes before attempting to replace it. BioTek Instruments, Inc. -
Page 17: Precautions
Precautions The following precautions are provided to help avoid damage to the instrument: Caution: Service . The instrument should be serviced by BioTek-authorized personnel. Only qualified technical personnel should perform service procedures on internal components. Caution: Spare Parts. -
Page 18: Ce Mark
EN 61010-1, “Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements.” EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.” EN 61010-2-010, “Particular requirements for laboratory equipment for the heating of materials.” BioTek Instruments, Inc. -
Page 19: Directive 2002/96/Ec: Waste Electrical And Electronic Equipment
Communications. Le present appareil numerique n'émet pas du bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada. ELx808 Operator’s Manual... -
Page 20: User Safety
Part 1: General requirements.” • Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.” • EN 61010 Standards, see CE Mark starting on page xvi. BioTek Instruments, Inc. -
Page 21: Safety Symbols
Attenzione, rischio di schiacciare ed intrappolarsi Warning, hot surface Warning, potential biohazards Attention, surface chaude Attention, risques biologiques potentiels Warnen, heiße Oberfläche Warnung! Moegliche biologische Giftstoffe Precaución, superficie caliente Atención, riesgos biológicos Attenzione, superficie calda Attenzione, rischio biologico ELx808 Operator’s Manual... - Page 22 Rayonnement laser: Ne pas regarder dans le faisceau Gebrauchsanweisung beachten Laserstrahlung: Nicht in den strahl Consultar las instrucciones de blicken Radiación de láser: No mire fijamente al Consultare le istruzioni per uso rayo Radiazione di laser: Non stare nel fascio BioTek Instruments, Inc.
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Page 23: Chapter 1: Introduction
Chapter 1 Introduction This chapter introduces the ELx808 Absorbance Microplate Reader and describes its hardware and software features, and technical specifications. Instructions for contacting BioTek are provided on page 7. ELx808 Absorbance Microplate Reader ........2 Reader Variations ............2 Hardware Features ............. -
Page 24: Introducing The Elx808 Absorbance Microplate Reader
| Chapter 1: Introduction ELx808 Absorbance Microplate Reader BioTek’s ELx808 is an eight-channel reader-assay system. The reader can serve as a standalone system, or can be controlled via BioTek’s Gen5 software. Designed to automatically perform endpoint and kinetic analysis, the reader can measure the optical density of solutions in 96-well microplates between 380 nm and 900 nm. -
Page 25: Software Features
• 55 assays are available onboard; up to 75 custom assays can be preprogrammed and exported to the reader using BioTek’s Define Protocol software. • Automatically stores results for the last 10 plates. Package Contents Package contents and part numbers are subject to change. -
Page 26: Specifications
Gen5 Software (visit biotek.com or contact your local dealer for details) • Absorbance Liquid Test Solutions: BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; PN 7120782, 125 mL) BioTek wetting agent (PN 7773002) • Adapter to connect the reader to a USB-only printer (PN 75135) -
Page 27: Environmental
13 seconds rapid mode; 20 seconds normal/regular mode • 6-second minimum kinetic interval, Single wavelength higher than 400 nm: rapid mode Standard Model: ELx808 The following specifications apply only to standard 96-well, flat- or round-bottom microplates. • Wavelength Range : 380 to 900 nm •... -
Page 28: Ultraviolet/Incubator Model - Elx808Iu
Incubation : The following specifications apply to a 96-well sealed plate with 200 μL of liquid in all wells: Temperature Control : Temperature controlled to 50°C Temperature Variation : ± 0.50°C @ 37°C with the plate sealed BioTek Instruments, Inc. -
Page 29: Product Support & Service
If your instrument or software fails to function properly, if you have questions about how to use or maintain your products, or if you need to send an instrument to BioTek for service or repair, please contact our Technical Assistance Center (“TAC”). - Page 30 | Chapter 1: Introduction BioTek Instruments, Inc.
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Page 31: Chapter 2: Installation
10: Install Software/Connect to Computer (Optional) .... 21 Attach the Cable ............21 Install Gen5 Software on the Host Computer ....21 Establish Communication ..........22 Changing the Baud Rate on the ELx808 ......22 11: Verify Performance ............. 23 Before Repackaging the Instrument ........24 ... -
Page 32: Product Registration
If the reader is damaged: Notify the carrier and your BioTek sales representative. Keep the shipping cartons and the packing materials for the carrier’s inspection. BioTek will arrange for repair or replacement of your reader immediately. (Refer to Figure 1 on the next page.) Carefully open the top of the box, and... -
Page 33: 2: Select The Operating Environment
Place all packing material back into the shipping box for reuse if the instrument needs to be shipped again. Figure 1: Unpacking the ELx808 2: Select the Operating Environment For best operation, install the reader on a level surface in an area where ambient temperatures between 18°C (64°F) and 40°C (104°F) can be maintained. -
Page 34: 3: Install The Filter Wheel
3: Install the Filter Wheel The filters that ship with the ELx808 are installed in the six-position filter wheel. For example, the standard models have 405, 450, 490, 630 nm filters; the UV model’s filter set is 405, 450, 490, 630 and 340 nm. - Page 35 The wheel should easily disconnect from the hub. Lift the wheel from the instrument. To properly store interference filters for the ELx808 during extended periods of non-use, package the filters in a light-tight envelope or container, away from high humidity and direct sunlight.
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Page 36: 4: Check/Adjust The Power Input Voltage Setting
Warning! Electrical Grounding . Never use a plug adapter to connect primary power to the ELx808 power supply. Use of an adapter disconnects the utility ground, creating a severe shock hazard. Always connect the power supply cord directly to an appropriate receptacle with a functional ground. -
Page 37: (Optional) Connect Printer
6: (Optional) Connect Printer | 6: (Optional) Connect Printer If the ELx808 will operate in standalone mode (that is, without BioTek’s Gen5 software running on a host PC), connect a printer directly to the reader using the supplied cable. Important! To avoid system instability, make sure the printer and reader are turned OFF before connecting them. -
Page 38: Printers
If all of the required steps preceding this one have been performed successfully, locate the reader’s power switch (on the right side) and turn on the reader. The ELx808 will automatically perform a System Test. The System Test conducts a series of tests at each... -
Page 39: 8: Check/Adjust The Reader's Filter Table
8: Check/Adjust the Reader’s Filter Table | 8: Check/Adjust the Reader’s Filter Table After installing the filter wheel (or new filters), ensure that the ELx808’s filter table accurately maps the physical location of the filters in the filter wheel. Turn on the reader if it is not already on. -
Page 40: 9: Configure Reader Settings
| Chapter 2: Installation 9: Configure Reader Settings The ELx808 may be configured a number of ways, depending on user preference. Configuration options are accessed via the Select Utility Option menu, and include: • SETUP : Set the date and time. -
Page 41: Output Options
C O L U M N M A T R I X B O T H The current selection appears on the top line of the display. Select the desired option (COLUMN, MATRIX, BOTH) to change the Report Type. ELx808 Operator’s Manual... -
Page 42: Read Options
4-character alphanumeric name. The starting ID is entered and automatically incremented by the software. Select to present this prompt at run-time. P R O M P T S A M P L E C O U N T ? Y E S BioTek Instruments, Inc. -
Page 43: Read Speed
Chapter 1 10: Install Software/Connect to Computer (Optional) The ELx808 has serial (RS232) port located on back of the reader (see on page 15). Figure 3 This port allows the reader to communicate with a computer using the BioTek Gen5 software. -
Page 44: Establish Communication
If you remain unable to get Gen5 and the reader to communicate with each other, contact BioTek’s Technical Assistance Center. Changing the Baud Rate on the ELx808 Gen5 requires the baud rate to be set to 9600. If you need to change the baud rate from the default of 9600 to either 1200 or 2400: Turn on the instrument if it is not already on. -
Page 45: 11: Verify Performance
To view the reader’s settings for parity, stop bits, and data bits, select VIEW 11: Verify Performance Before using the ELx808 for the first time, verify that the reader is operating properly by running a System Test and the Absorbance Plate Test. These tests and additional verification procedures are described in Chapter 4, Instrument Qualification ELx808 Operator’s Manual... -
Page 46: Before Repackaging The Instrument
This shipping system was designed to be used no more than five times. If the container is damaged and/or has been used more than five times, contact BioTek for a new set of shipping materials, and ask for PN 7343000. -
Page 47: Chapter 3: Operation
Chapter 3 Operation This chapter includes instructions for operating the ELx808 Reader and its software. Getting Started with Gen5 ..........27 Introduction ..............28 The Keypad ..............29 The Startup Screen ............30 The Main Menu Screen ..........30 Quick Read ..............31 Defining Assays ............... - Page 48 Some readers have custom programmed assays installed. Not all features of the software discussed in this chapter are available on custom instruments. Please contact BioTek’s Technical Assistance Center (TAC) if you have any questions about the assays on your reader...
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Page 49: Getting Started With Gen5
Plate screen to select one wavelength. The results will be displayed for the selected wavelength. Repeat, for other wavelengths. • To analyze, manipulate, or print results, Protocol parameters should be defined. Refer to the Gen5 Help system for instructions. ELx808 Operator’s Manual... -
Page 50: Introduction
| Chapter 3: Operation Introduction The front panel on the ELx808 Reader features a 25-pad keypad and a 2-line x 24-character LCD display as the user interface (see Figure 4 below). The reader’s bidirectional serial port allows computer control of the instrument and provides the means for downloading additional assay protocols to the instrument. -
Page 51: The Keypad
Press the ► (forward) arrow to move the cursor to the right in the LCD ► display. To start running a reader program, press the Read key. READ To stop running (abort) a reader program, press the Stop key. STOP ELx808 Operator’s Manual... -
Page 52: The Startup Screen
| Chapter 3: Operation The Startup Screen To turn on the ELx808, press the on/off switch on the right side of the reader’s base. The ELx808 will perform a system self-test, displaying the screens shown below until initialization is complete. During this time, all keys are inactive. -
Page 53: Quick Read
96-well plate geometry • Blank on Air • Automap • Map starting location A1 • Samples only (no blanks, standard, or controls) • Sample count prompted at runtime (can be turned off in UTIL > READ options) ELx808 Operator’s Manual... -
Page 54: Defining Assays
At the Assay Name screen, edit the name assigned to the Assay. The assay name can be up to 16 characters. E D I T > H B S - A G 1 S P A C E BioTek Instruments, Inc. -
Page 55: Defining The Method, Map, Formula, And Curve
SOFT KEY 4 CURVE FIT is selected and the user is prompted for curve-fit options. • In addition, the MAIN MENU PREVIOUS SCREEN ENTER keys are active, allowing you to move back and advance through the menu structure. ELx808 Operator’s Manual... -
Page 56: Method
F I R S T R E A D T I M E : X X : X X • Enter the time in minutes and seconds, using the numeric keys. • to advance to the screen. Press Enter INCUBATION TEMPERATURE BioTek Instruments, Inc. -
Page 57: Incubation Temperature
Dual wavelength readings can significantly reduce optical interference caused by scratched or fingerprinted microplates since the scratches or fingerprints reduce the amount of light on both wavelengths. • Press Enter to save the selection and advance to the next screen. ELx808 Operator’s Manual... -
Page 58: Meas Selection
Valid ranges are: 0-1 hours, 0-59 minutes and 0-59 seconds. The number of Reads = Duration/Interval must be less than or equal to 40 and more than or equal to 2. BioTek Instruments, Inc. -
Page 59: Kinetic Number Of Reads
• Press SOFT KEY 3 to select shaking. • Press to save the selection and advance to the next screen. ENTER • Press SCREEN key to save the selection and return to a previous screen. ELx808 Operator’s Manual... -
Page 60: Shake Time
R - S Q R O N S E T • Press SOFT KEY 1 to select the kinetic rate calculation. This method will apply a linear fit to calculate the maximum slope based on the number of kinetic points specified. BioTek Instruments, Inc. -
Page 61: Number Of Kinetic Points Selection
E N T E R N U M B E R S C A N P O I N T S : ELx808 Operator’s Manual... -
Page 62: Map
Concentration and Location of Standards • Number of Controls • Control Type Definition • Number of Control Replicates • Control Location • Number of Samples • Number of Sample Replicates • Averaging of Samples • Sample Location BioTek Instruments, Inc. -
Page 63: Map Generation
SOFT KEY 2 MANUAL plate map generation. The display updates to reflect the selection. • Press ENTER to save the selection and move to the next screen. Note: Press SHIFT-CLEAR to clear any previously defined manual map. ELx808 Operator’s Manual... -
Page 64: Mapping Direction
B1. The next standard control, or sample, would follow in B2. • Press ENTER to save the selection and advance. Examples of mapping and replication directions are shown on the next page. BioTek Instruments, Inc. -
Page 65: Examples Of Mapping & Replication Directions
STD3 STD4 STD4 STD5 STD5 Map Direction DOWN, Rep Direction ACROSS: STD1 STD1 STD2 STD2 STD3 STD3 STD4 STD4 STD5 STD5 Map Direction ACROSS, Rep Direction DOWN: STD1 STD2 STD3 STD4 STD5 STD1 STD2 STD3 STD4 STD5 ELx808 Operator’s Manual... -
Page 66: Start Mapping At Well Location
* M O R E B L A N K M A P : F U L L P - A C R O S S P - D O W N * M O R E BioTek Instruments, Inc. -
Page 67: Blank Map Definitions
A, C, E and G rows. Manual mapping is recommended to set up the appropriate map by placing the standards, controls, and samples in only the A, C, E, and G rows. ELx808 Operator’s Manual... -
Page 68: Constant Blank Value Entry
T H E L O C A T I O N B L A N K # 1 : A 1 2 • Use the NUMERIC ALPHA keys to enter a Blank Location, based upon the plate geometry. BioTek Instruments, Inc. -
Page 69: Number Of Standards
The Average Standards option allows you to select whether or not to average the Replicates of a Standard. This average is used to calculate the standard curve instead of using the individual replicate of each standard. Note: If the replicate selection is 1, this option is not available. ELx808 Operator’s Manual... -
Page 70: Standard Concentration
• Press the CLEAR key to clear the Standard Concentration value from the display. • Press the ENTER key to save the value on the display and move to the next screen. BioTek Instruments, Inc. -
Page 71: Reuse Of Standard Curves
Defining Assays | Reuse of Standard Curves The ELx808 has the ability to reuse a standard curve that has already been established. Limitations of the Reuse of Standard Curves • The reuse of standard curves can only be done in assay positions 31 through 55. -
Page 72: Number Of Controls
L P C C T L 1 C T L 2 * M O R E C O N T R O L # 1 : C T L 3 C T L 4 * M O R E BioTek Instruments, Inc. -
Page 73: Number Of Control Replicates
For all prompts of a well location, only the ALPHA keys are active for the first character and NUMERIC for the second and third characters. • Press the MAIN MENU PREVIOUS SCREEN keys to move back and advance through the menu structure. ELx808 Operator’s Manual... -
Page 74: Number Of Samples
L O C A T I O N R E P # 1 : A 0 1 • Use the NUMERIC ALPHA , and DECIMAL POINT keys to enter the sample identifier and its location on the plate. BioTek Instruments, Inc. -
Page 75: Formula
FORMULA The ELx808 supports three types of formulas (Cutoff, Transformation, and Validation), as well as the ability to define variables for use within formulas. Up to three types of Validation formulas may be defined (Blank, Control, and Assay Validation). -
Page 76: Formula Type
| Chapter 3: Operation Formula Type The ELx808 supports three types of formulas, as well as the ability to program variables for use within Transformation formulas. • CUTOFF formulas are used to classify results. During data reduction, results are evaluated against the cutoff formulas, and each well is assigned a user-specified label or “call”... -
Page 77: Validation Type Selection
SOFT KEY 1 MATH position. The following table shows the order of items on the MATH list: Addition sign Subtraction sign Multiplication sign Division sign Percent ELx808 Operator’s Manual... - Page 78 SOFT KEY 4 to place the next option on the FUNCTION list at the cursor position. The available functions are: LOG10 Log Base 10 ALOG1 Anti Log Base 10 Absolute Value Power ALOG Anti Log SQRT Square Root BioTek Instruments, Inc.
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Page 79: Number Of Required Controls/Blanks
Press the Clear key to clear the displayed value. • Press the Enter key to save the displayed value and advance to the next screen, or use the Previous Screen key to move backward through the menu structure. ELx808 Operator’s Manual... -
Page 80: Cutoff Formulas
O T H E R M A P F U N C T N After the FORMULA #1 : prompt disappears, enter the formula as described in “Formula Entry” on page 55. Refer to the examples on page 60. BioTek Instruments, Inc. -
Page 81: Greyzone Entry
If, for example, POS is selected as shown in the above screen, calls will be assigned according to the following equations (SMP represents the sample wells): EQUIV: SMP <= (CUTOFF+(CUTOFF*GREYZONE)) AND SMP >= (CUTOFF-(CUTOFF*GREYZONE)) POS: SMP > (CUTOFF+(CUTOFF*GREYZONE)) NEG: SMP < (CUTOFF-(CUTOFF*GREYZONE)) ELx808 Operator’s Manual... -
Page 82: Examples
Calls are assigned to sample wells as follows: EQUIV if the sample is greater than or equal to 1.800 and less than or equal to 2.200 POS if the sample is greater than 2.200 NEG if the sample is less than 1.800 BioTek Instruments, Inc. -
Page 83: Transformation Formulas
Divide all ODs on the plate by 2 and multiply by 100. Enter the formula: (OD/2)*100 This formula will be applied to the ODs of all samples, standards, controls, and blanks that are present on the plate map. ELx808 Operator’s Manual... -
Page 84: Transformation Scope Variable
Use the formula keys (Math, Other, Map and Function) to define the ). Once the variable has been transformation variable TVar defined, it can be used in a transformation formula. The TVar will be available as a MAP option when writing the transformation formula. BioTek Instruments, Inc. -
Page 85: Another Transformation Example
0 standard), and multiplies the results by 100. • At the SCOPE VARIABLE screen, choose OD and press Enter • Select from and Press STD1 Enter • Choose TRANS • Enter ( OD/TVAR ) * 100 ELx808 Operator’s Manual... -
Page 86: Curve
• Extrapolation of Unknowns These screens are displayed on the ELx808 in the order in which they appear in the assay. If a closed variable (i.e., an element of the assay definition that you cannot access or modify) is being used in the assay, the entry screen is omitted. -
Page 87: Edit Standard Outliers
M A N U A L • Press SOFT KEY 1 to select the edit option displayed above the soft key. The display updates to reflect your selection. • Select NONE to suppress the Edit Standard Outliers capability for this assay. ELx808 Operator’s Manual... -
Page 88: Axis Selection
• Press SOFT KEY 1 to select (extrapolate the unknowns). The top line of the display updates to reflect this selection. • On the printed reports, extrapolated concentrations (RSLT values) are surrounded by < > (e.g., <44.425>). BioTek Instruments, Inc. -
Page 89: Panel Assays
Panel. To create a panel assay, start at the Main Menu, select DEFINE , then choose assay number . Enter the panel assay name. N A M E : P A N E L ELx808 Operator’s Manual... - Page 90 Use the alpha and numeric keys to choose the well location to begin the assay. • Repeat this process for each assay within the panel. Remain aware of the total number of controls, standards, and blanks that were originally mapped in each individual assay while mapping for the Panel assay. BioTek Instruments, Inc.
- Page 91 Assay 8 mapping is complete. The Panel Assay results are sorted by Sample (unless a custom assay has been programmed by BioTek). Note: The Interpretation of Results reports for each assay in the Panel will print first, and then the Sample results will print.
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Page 92: Reading A Microplate
• If running a custom assay, typical prompts might include: Number of samples Standard concentrations Assay ID Fill pattern Blank method First well location Replicate count for each well type BioTek Instruments, Inc. -
Page 93: Enter Number Of Samples
You can start entering a starting sample identification number from 0001 to 9999. The software will automatically increment each subsequent sample identification number by 1. Sample IDs will be assigned according to the previously defined mapping order. ELx808 Operator’s Manual... -
Page 94: Prompts For Well Location
After the plate has been read, all requested reports will be generated. • To halt in read in progress, press the STOP key. Note: If using the incubation option, the reader will wait for the incubator to reach temperature before reading the plate. BioTek Instruments, Inc. -
Page 95: Printing Reports And Assay Lists
OD range defined for the assay. For the Quick Read assay on the ELx808, this range is -3.0 OD to +3.0 OD. For assays defined onboard by a user (Assay02-Assay55), it is -4.0 OD to +4.0... -
Page 96: Result
• Select to retain the replicate. • Press ENTER to advance to the next replicate. AVERAGE STANDARDS was set to in the assay definition, one or more standard groups can be chosen for exclusion. BioTek Instruments, Inc. -
Page 97: Printing Results
N A M E : H B S - A G • Press Options to cycle through the list of available assays, or enter the number of the desired assay. • Press Enter to print the report. ELx808 Operator’s Manual... -
Page 98: Assay
Main Menu, and then select . The entire list of REPORT LIST assays stored in the ELx808’s memory will be sent to the printer. Recommendations for Optimum Performance • Microplates should be perfectly clean and free of dust or bottom scratches. Use new microplates from sealed packages. - Page 99 Because of the mathematical correction, this difference under normal conditions should be slight or nonexistent. • A 10-minute warm-up of the instrument is suggested, prior to reading, to achieve the best repeatability from microplate-to-microplate measurements. ELx808 Operator’s Manual...
- Page 100 | Chapter 3: Operation BioTek Instruments, Inc.
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Page 101: Chapter 4: Instrument Qualification
Chapter 4 Instrument Qualification This chapter discusses the tasks and procedures necessary for qualifying instrument performance initially and on an ongoing basis. Overview ................ 80 Recommended Qualification Schedule ......... 80 Test Descriptions ............. 81 System Test ............... 81 Checksum Test ............84 Absorbance Plate Test .......... -
Page 102: Overview
Operational Qualification (OQ), and Performance Qualification (PQ) procedures for all models of the ELx808 Absorbance Microplate Reader. Every ELx808 reader is fully tested at BioTek prior to shipment and should operate properly upon initial setup. If you suspect that a problem occurred during shipment, if... -
Page 103: Test Descriptions
(if equipped) proper function of the incubator. The testing is designed to verify that the ELx808 will give in-specification performance for each set wavelength over the specified OD range. - Page 104 9413 8655 8927 Delta: 11798 20186 38931 32693 19137 21661 15731 27860 23019 Channel: Noise Max: 3952 4351 4225 4817 4668 4790 5237 4807 4963 Noise Min: 3951 4346 4220 4814 4664 4785 5233 4802 4957 Delta: BioTek Instruments, Inc.
- Page 105 Tel: 802 655 4040 Fax: 802 655 3399 email: TAC@biotek.com Product support center: www.biotek.com/service Figure 5: Sample System Test Report (it varies slightly depending on how it was run) If a System Test shows one or more channels that have air readings falling below 30,000 for filter wavelengths of 405 nm or higher, Liquid Test 1 should be performed.
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Page 106: Incubator Self Test
Current Average: Average temperature of the four zones of the incubator at the time the system test is run. This number is displayed on the ELx808 front panel and sent to Gen5 if the reader is being computer controlled. •... -
Page 107: Absorbance Plate Test
NIST-traceable values. To run the test, you will need a BioTek Absorbance Test Plate with its accompanying data sheet. The BioTek PN 7260522 Test Plate has a glass filter in location C6, which is used to check the wavelength accuracy of a monochromator. Although... - Page 108 | Chapter 4: Instrument Qualification Figure 6: Sample Absorbance Plate Test Report The report varies slightly depending on how it was run (via the keypad or Gen5). BioTek Instruments, Inc.
- Page 109 If there are any mismatches, correct them and re-run the test. See also the troubleshooting tips below. If the test continues to fail, contact BioTek TAC. Please have a copy of the test and the reader’s serial number ready.
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Page 110: Empty Carrier Test
Delta values are adjusted relative to the signal strength of the reference channel. Empty Carrier Test The Empty Carrier Test confirms the ELx808’s read capabilities at the 100% light level, and can help to identify deteriorating interference wavelength filters and other optical problems. -
Page 111: Perform The Tests
Checksum Test There is no Checksum Test in Gen5. To obtain reader software information: • Open the system menu and select Reader Control > ELx808 . To obtain Gen5 software information, select Help > About Gen5 Using the instrument keypad: •... -
Page 112: Run The Plate Test
The Test Plate and utility do not correct or calibrate the instrument. The Absorbance Plate Test tests the accuracy and repeatability of the reader to 2.500 OD. It does not indicate a PASS or FAIL above 2.500 OD. BioTek Instruments, Inc. -
Page 113: Empty Carrier Test
If the Empty Carrier test fails at just one wavelength: • Remove the filter wheel and examine the filter that exhibited the problem; it may need to be replaced (contact BioTek). Check for spotting or a halo effect. ELx808 Operator’s Manual... -
Page 114: Liquid Tests
Liquid Tests Prepare Test Solutions BioTek offers a dye solution (PN 7120779, 25 mL; or 7120782, 125 mL) that may be used in the stock solution formulation for Liquid Tests 1 and 2, or, if you prefer, you may use the dye solution described below. The purpose of the formulation is to create a solution that absorbs light at ~2.000 OD full strength when dispensed at... -
Page 115: Solution B
5-mL Class A volumetric pipette • 100-mL volumetric flask Prepare Stock Solution: Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask. Add 95 mL of DI water; cap and shake well. Solution B Required Materials: •... -
Page 116: Perform Liquid Test 1
± 1.0% ± 0.010 OD, then the expected range for the mean of the same well in its Turnaround (H12) position is 1.873 to 1.931 OD. 1.902 * 0.010 + 0.010 = 0.029; 1.902 – 0.029 = 1.873 ; 1.902 + 0.029 = 1.931 BioTek Instruments, Inc. -
Page 117: Accuracy Specifications
80% in the third tube, all the way to 10% in the tenth tube. Dilute using the 0.05% solution of deionized water and Tween ® 20. This solution can also be made by diluting the BioTek wetting agent 200:1. ELx808 Operator’s Manual... -
Page 118: Perform The Linearity Test (Test A)
Do not discard the plate; you will use it again for Test C. Print the ten sets of raw data or export them to an Excel spreadsheet using Gen5. The mathematical computations described below may then be performed and the template kept for future data reduction. BioTek Instruments, Inc. -
Page 119: Calculate Repeatability (Test B)
(Compare B1 to G12, C1 to F12, D1 to E12, E1 to D12, F1 to C12, G1 to B12, H1 to A12). The difference in the values for any two corresponding wells ELx808 Operator’s Manual... -
Page 120: Perform Liquid Test 3 ("Uv" Models Only)
Liquid Test 3 is an optional test offered for sites that must have proof of linearity at wavelengths lower than those attainable with the Absorbance Test Plate. This test verifies operation of the ELx808 at 340 nm and is optional because the reader has good “front-end”... -
Page 121: Perform The Test
The well that shows the most variation for each concentration is selected for data reduction. For each mean calculated in Step 1, calculate the allowed deviation using the repeatability specification for a 96-well plate of ± 1.0% ± 0.005 OD from 0.000 to 2.000 OD at 340 nm. ELx808 Operator’s Manual... -
Page 122: Calculate Linearity
Perform a regression analysis on the data to determine if there is adequate linearity. Since it is somewhat difficult to achieve high pipetting accuracy when conducting linear dilutions, an R Square value of at least 0.99 is considered adequate. BioTek Instruments, Inc. -
Page 123: Chapter 5: Preventive Maintenance
Chapter 5 Preventive Maintenance This chapter provides step-by-step instructions for maintaining the ELx808 in top condition, to ensure that the reader continues to perform to specification. Recommended Maintenance Schedule ........ 102 Warnings and Precautions ..........102 Clean Exposed Surfaces ........... 103 ... -
Page 124: Recommended Maintenance Schedule
| Chapter 5: Preventive Maintenance Recommended Maintenance Schedule A general Preventive Maintenance (PM) regimen for all ELx808 models includes periodically cleaning all exposed surfaces, inspecting/cleaning the wavelength filters, replacing the lamp, and decontamination. Robotic models should have certain components lubricated. The table below recommends Preventive Maintenance tasks and the frequency with which each task should be performed. -
Page 125: Clean Exposed Surfaces
Clean Exposed Surfaces Important! Turn off and unplug the ELx808 for the cleaning procedure. Exposed surfaces may be cleaned (not decontaminated) with a cloth moistened (not soaked) with water or water and a mild detergent. You will need: •... -
Page 126: Inspect And Clean The Wavelength Filters
Inspect the glass filters for speckled surfaces or a halo effect. This may indicate deterioration due to moisture exposure over a long period of time. If you have any concerns about the quality of the filters, contact your BioTek representative. -
Page 127: (Optional) Lubricate Robotic Components
BioTek Representative or BioTek. (Optional) Lubricate Robotic Components This section applies to ELx808 robotic models only. You will need lubricant, BioTek PN 66039. Remove the top shroud of the instrument (see instructions in... - Page 128 | Chapter 5: Preventive Maintenance Figure 8a: Location of bracket, motor shaft, and main PCB/motor assembly Figure 8b: Roller surface and hook-in bracket BioTek Instruments, Inc.
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Page 129: Robotic Motor Adjustment Procedure
The intensity of the lamp will slowly drop over time until the reader’s run-time self-check detects a low signal and warns the user with a displayed error code. The bulb should be replaced at this time (PN 3400508), and the electrical contacts in the lamp socket cleaned. ELx808 Operator’s Manual... - Page 130 Cotton swabs or pencil eraser • Isopropyl alcohol • Lamp, BioTek PN 3400508 To replace the lamp: Turn off and unplug the reader. Allow the lamp to cool for at least ten minutes. Temporarily tape the reader’s plate access door shut.
- Page 131 Ensure that the bulb is set flat against its housing before reinstallation in the instrument. O-ring lamp clamp washers thumbscrews springs Lamp Assembly 1 Figure 9: New lamp hardware for the ELx808 ( ELx808 Operator’s Manual...
- Page 132 | Chapter 5: Preventive Maintenance Lamp Assembly 1 Figure 10: New lamp hardware for the ELx808, assembled ( Lamp Assembly 2 Figure 11: Location of the lamp in the ELx808 ( BioTek Instruments, Inc.
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Page 133: Decontamination
Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle. -
Page 134: Tools And Supplies
Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Do not soak the keypad – this will cause damage. - Page 135 Use a clean, dry lint-free cloth to dry all wet surfaces. Reassemble the instrument as necessary. Discard the used gloves and cloths, using a Biohazard trash bag and an approved Biohazard container. ELx808 Operator’s Manual...
- Page 136 | Chapter 5: Preventive Maintenance BioTek Instruments, Inc.
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Page 137: Chapter 6: Troubleshooting And Error Codes
Troubleshooting and Error Codes This chapter provides a list of Error Codes that may appear on the ELx808 or in Gen5, and their probable causes. Overview ............... 116 Diagnostics ..............116 Terms Used in the Error Codes Tables ......116 Error Codes (General) ............. -
Page 138: Overview
| Chapter 6: Error Codes Overview An error code that appears on the display of the ELx808 (during instrument control of the reader), or in Gen5 software (during PC control of the reader) is displayed as a four-digit identifier. The first digit will be 0, 1, 2, or A. -
Page 139: Error Codes (General)
Probable causes: Mechanical obstruction. • • Carrier belt or pulley slipping. Loose filter wheel hub. • Loose filter may be impeding filter wheel movement. • Defective motor drive circuit or 12-volt power problem. • ELx808 Operators’ Manual... - Page 140 Missing or loose filter allowing unintended lamp light into the • measurement chamber. Check filter wheel for empty positions. Internal electronic noise caused by a faulty Analog PCB or faulty • internal grounding. BioTek Instruments, Inc.
- Page 141 Failed configuration checksum test. Re-download software basecode and assays. 1200 Calibration data missing. 1201 Calibration value missing Filter table was updated but System Test was not run. Run a system test. 1300 Motor not homed. Autocal error, see error 0200. ELx808 Operators’ Manual...
- Page 142 Electrical noise or grounding issue. • Shortened lamp life from performing long kinetic runs every day or • leaving the reader on 24 hours a day. When using 340 nm filters, the analog gain must be increased. • BioTek Instruments, Inc.
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Page 143: Error Codes (Fatal)
Error Codes (Fatal) | Error Codes (Fatal) These errors indicate conditions that require immediate attention. If a fatal error is displayed, contact BioTek’s Technical Assistance Center for further instructions (refer to section in Product Support & Service Chapter 1 Code... - Page 144 | Chapter 6: Error Codes BioTek Instruments, Inc.
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Page 145: Appendix A: Sample Reports
OD for a group of wells, falls outside the minimum/maximum OD range defined for the assay. For the Quick Read assay on the ELx808, this range is -3.0 OD to +3.0 OD. For assays defined onboard by a user (Assay02- Assay55), it is -4.0 OD to +4.0 OD. -
Page 146: Samples With Calls On Matrix Report
| Appendix A: Sample Reports Samples with Calls on Matrix Report BioTek Instruments, Inc. -
Page 147: Curve Fit Report
Curve Fit Report | 125 Curve Fit Report ELx808 Operator’s Manual... -
Page 148: Samples With Calls On Column Report
| Appendix A: Sample Reports Samples with Calls on Column Report BioTek Instruments, Inc. -
Page 149: Column Report Without Samples
Column Report without Samples | 127 Column Report without Samples ELx808 Operator’s Manual... -
Page 150: Panel Report
| Appendix A: Sample Reports Panel Report BioTek Instruments, Inc. -
Page 151: Assay Detail Report
Assay Detail Report | 129 Assay Detail Report ELx808 Operator’s Manual... -
Page 152: Assay List
ASSAY 12 ASSAY 13 ASSAY 14 ASSAY 15 ASSAY 16 ASSAY 17 ASSAY 18 ASSAY 19 ASSAY 20 ASSAY 21 ASSAY 22 ASSAY 23 ASSAY 24 ASSAY 25 ASSAY 26 ASSAY 27 ASSAY 28 ASSAY 29 ASSAY 30 BioTek Instruments, Inc. -
Page 153: Appendix B: Instructions For Programming A New Assay
The kit instructions are provided so that users can see how it is possible to translate the kit wording into an ELx808 assay program. For clarity, only the user menu choices from the reader screens are shown. See Chapter 3 for details. - Page 154 • Type III Sample Diluent: 2 bottles containing 50 mL of buffer for sample dilution. Ready to use. • ANA Positive Control: 1 bottle containing 1.8 mL of diluted stabilized serum. Ready to use. BioTek Instruments, Inc.
- Page 155 If the above criteria are not met, the assay is invalid and the test should be repeated. Calculation of the Sample Results Use the following formula to calculate the ANA result for each sample: Control or sample OD x 10 = Control or sample value (U/mL) Cutoff control OD ELx808 Operator’s Manual...
- Page 156 ANA Result Interpretation < 10.0 Negative > 10.0 Positive Programming the Assay on the ELx808 From the Main Menu, press . Enter the assay number and edit the name if DEFINE desired. At the DEFINE menu: STEP...
- Page 157 TRANS: FORMULA: (OD/TVAR)*10 Converts all OD values on plate to ”ANA Result” per the kit instructions. To define a cutoff formula for Positive and Negative calls: FORMULA CUTOFF: 10.0 GREYZONE: 00% SAMPLE > CUTOFF: POS ELx808 Operator’s Manual...
- Page 158 Cardiolipin Coated Wells: 12 break-apart 8-well strips coated with bovine cardiolipin antigen. The plate is packaged in a re-sealable foil bag containing two desiccant pouches. • Type II Sample Diluent: 2 bottles containing 50 mL of buffer for sample dilution. Colored yellow, ready to use. BioTek Instruments, Inc.
- Page 159 The values obtained for all the controls should be in the ranges specified on the QC Certificate. • The curve shape should be similar to the standard curve, shown on the QC Certificate. • If the above criteria are not met, the assay is invalid and the test should be repeated. ELx808 Operator’s Manual...
- Page 160 1 µg/mL of an affinity purified IgG anticardiolipin preparation from a standard serum. The Louisville reference center recommends the following positive discrimination criteria according to the recommendation of the 2nd International Anticardiolipin Workshop. BioTek Instruments, Inc.
- Page 161 Anticardiolipin < 11 GPL U/mL Negative result > 11 GPL U/mL Positive result Programming the Assay on the ELx808 From the Main Menu, press DEFINE . Enter the assay number and edit the name if desired. At the DEFINE...
- Page 162 To define a cutoff formula for Positive and Negative calls: Note: Kit instructions specify that samples with concentration values greater than 11 should appear as positive. The ELx808 software calculates the cutoff based on absorbance value or transformed value (see the previous example) and cannot calculate based on concentration.
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Page 163: Appendix C: Adjusting The Power Input Voltage Setting
This appendix does not apply to ELx808 readers equipped with an external power supply. This appendix provides instructions for adjusting the power input voltage setting in ELx808 instruments that are equipped with an internal four-voltage range power input module. Identifying the Reader’s Power Input Module ...... 142 ... -
Page 164: Identifying The Reader's Power Input Module
Previous versions of the ELx808 (models manufactured before December 2005) have an internal, four-voltage range power input module, instead of an external power supply. Located on the right side of the ELx808, the input module can be adjusted for a 100, 120, 230, or 240 V~ voltage setting. -
Page 165: Newer-Style Module
As shown below, a small voltage selector card is located to the right of the fuse holder. Using the pliers, carefully pull this card straight out of the compartment. Figure 12: Power input module: accessing the voltage selector card and fuse holder ELx808 Operator’s Manual... - Page 166 ON/OFF switch, and the desired voltage range is on the bottom of the card (see above). Reconfigure or replace fuses as needed (see next page). Replace the cover, and verify that the white peg lines up with the correct voltage indicator hole. BioTek Instruments, Inc.
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Page 167: Reconfigure Or Replace Fuses
A failed fuse is usually an indication of another problem that a new fuse is not likely to remedy. Contact BioTek’s Technical Assistance Center if the fuse replacement fails to resolve the problem. See Chapter 1 for contact information. - Page 168 Remove the defective fuse and replace it with the correct new one. Reinstall the fuse holder with the fuse toward the back of the power input module. Replace the cover, and verify that the white plastic indicator peg lines up with the correct voltage indicator hole. BioTek Instruments, Inc.
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Page 169: Older-Style Module
Important ! The peg indicating the intended voltage should protrude through the cover. Do not power up the instrument until the voltage range to be used is indicated correctly by the peg. ELx808 Operator’s Manual... -
Page 170: Reconfigure Or Replace The Fuses
Both USA and International style fuses are installed in the fuse holder that is attached to the inside of the power input module’s cover. The reader’s fuses are configured at the factory prior to shipping. Use the procedure below if you need to reconfigure or replace fuses. BioTek Instruments, Inc. - Page 171 Older-Style Module | 149 A failed fuse is usually an indication of another problem that a new fuse is not likely to remedy. Contact BioTek’s Technical Assistance Center if the fuse replacement fails to resolve the problem. See Chapter 1 for contact information.
- Page 172 | Appendix C: Adjusting the Power Input Voltage Setting BioTek Instruments, Inc.
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Questions and answers
Error 0911 when trying to read plate
Is it recommended to use the auto shake function when reading immunoassay plates after addition of TMB and stop solution