Related Manuals for bioMerieux Air IDEAL 3P
Summary of Contents for bioMerieux Air IDEAL 3P
- Page 1 ® Air IDEAL User's Manual LCB 71260 La Salle FRANCE bioMérieux SA Chemin de l'Orme 69280 Marcy-l'Étoile - France RCS Lyon 673 620 399 Tel. 33 (0)4 78 87 20 00 Fax 33 (0)4 78 87 20 90 www.biomerieux.com...
- Page 3 R e v i s i o n s The list of revisions below summarizes replacements or additional pages in your Air IDEAL 3P User’s Manual. Date of Version Modifications Page (s) modified printing 01/2001 Bilingual (FR / EN) 04/2001...
- Page 5 Users are invited to refer to these clauses. T r a d e m a r k s BIOMERIEUX, the blue logo, 3P, Air IDEAL 3P and Count-Tact are used, pending and/or registered trademarks belonging to bioMérieux, or one of its subsidiaries, or one of its companies.
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Page 7: Table Of Contents
Consumables ................................3-2 Culture media for general use .........................3-2 Irradiated media...............................3-2 Identification of the instrument ..........................3-3 Front face of Air IDEAL 3P ............................3-3 Rear face of Air IDEAL 3P.............................3-4 Keypad ..................................3-4 Configuration for 65, 70 or 90 mm Petri dishes .....................3-5 Tripod assembly ..............................3-6... - Page 8 Programming sequenced sampling (Menu 5) ......................3-24 Battery autonomy test (Menu 6) ..........................3-25 Starting sampling ..............................3-26 Stopping the motor ..............................3-28 Setting Air IDEAL 3P parameters (summary diagram)....................3-29 4 Procedure Principle ..................................4-1 Procedure................................... 4-1 How to obtain a good quality sample ......................... 4-2 Precautions of use..............................
- Page 9 Figure 3-5 : Diameter of Petri dishes ..........................3-5 Figure 3-6: Sampling head and grid – 90 mm Petri dishes ..................3-6 Figure 3-7: Tripod assembly ............................3-6 Figure 3-8: Air IDEAL 3P in its carrying case......................3-10 Figure 3-9: Sampling positions ..........................3-12 Figure 3-10: Screwing on the sampling grid......................3-13 ®...
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Page 11: How To Use This Manual
H o w t o u s e t h i s m a n u a l IMPORTANT! Please read the "General safety and regulatory information" booklet provided with the instrument. F i n d i n g t o p i c s a n d p r o c e d u r e s This manual is divided into 7 chapters. -
Page 12: Glossary
How to use this manual Glossary G l o s s a r y Acrylonitrile butadiene styrene. Instrument used to collect and count viable bacterial and fungal particles in a known and Aerobiocollector or precise volume of air. Air sampler Number of Colony Forming Units read on the agar plate. -
Page 13: Typographic Conventions
T h e A i r I D E A L 3 P k e y p a d There are 5 keys on the Air IDEAL 3P keypad. (See page 3-4 of the manual). In this manual, the keys are referred to by their individual names, enclosed in angle brackets "<... -
Page 15: Functional Description
Air IDEAL 3P evidently remains perfectly suited to the enumeration of airborne microorganisms in less demanding work environments such as agribusiness. In addition and in order to continue its universal application, Air IDEAL 3P is still available in two versions: −... -
Page 16: Performance
C o l l e c t i o n e f f i c i e n c y v a l i d a t e d a c c o r d i n g t o t h e I S O 1 4 6 9 8 s t a n d a r d Air IDEAL 3P was third party validated by the Health Protection Agency (UK) to meet the requirements of ISO 14698-1 for the control of clean rooms. -
Page 17: Using Air Ideal 3P
D e s c r i p t i o n Air IDEAL 3P is supplied in a carrying case. Inside the case there is a rigid lid which can be used as a small work surface [dimensions 22.7 x 13.9 cm (8.9 in. x 5.4 in.)]. -
Page 18: Consumables
Description C o n s u m a b l e s Air IDEAL 3P is adapted to all types of Petri dishes available on the market: 65, 70 and 90 mm. Moreover, the use of a large range of ready-to-use culture media – irradiated or classic –... -
Page 19: Identification Of The Instrument
Using Air IDEAL 3P Description I d e n t i f i c a t i o n o f t h e i n s t r u m e n t Figure 3-1: Identification of the instrument... -
Page 20: Rear Face Of Air Ideal 3P
Using Air IDEAL 3P Description R e a r f a c e o f Ai r I D E AL 3 P Handle Air outlet Threading for tripod Jack socket Figure 3-3: Rear face K e y p a d Figure 3-4: Keypad ®... -
Page 21: Configuration For 65, 70 Or 90 Mm Petri Dishes
Using Air IDEAL 3P Description C o n f i g u r a t i o n f o r 6 5 , 7 0 o r 9 0 m m P e t r i d i s h e s... -
Page 22: Tripod Assembly
Using Air IDEAL 3P Description Aspiration orifice Stainless steel strip for attachment Seal Figure 3-6: Sampling head and grid – 90 mm Petri dishes T r i p o d a s s e m b l y Figure 3-7: Tripod assembly ®... -
Page 23: General Specifications
Using Air IDEAL 3P General specifications G e n e r a l s p e c i f i c a t i o n s E n vi r o n m e n t a l c o n d i t i o n s Normal storage conditions ................-20°... -
Page 24: Technical Characteristics
− Possibility of suspending Air IDEAL 3P with a hook, especially in a glove box. − Optionally, Air IDEAL 3P can be mounted on a telescopic tripod equipped with a ball joint enabling the sampling axis to be orientated through an angle of 0° to 90° (from horizontal to vertical), and its height to be adjusted (between 0.7 and 2.50 meters). -
Page 25: Electrical Characteristics
- AC input voltage corresponding to the characteristics of the power supply in the country where the instrument is installed. Note: It is recommended to use the adapter provided with Air IDEAL 3P. You will find the details of its characteristics below. POWER SUPPLY UL/CSA INPUT.......... -
Page 26: Unpacking Air Ideal 3P
Do not use the instrument in an explosive atmosphere as sparks could cause an explosion. P a c k i n g l i s t − an Air IDEAL 3P aerobiocollector fitted with a protective cover. − 5 sampling grids − a hook for suspending the instrument −... -
Page 27: Recommendations For Installation And Use
Using Air IDEAL 3P Recommendations for installation and use R e c o m m e n d a t i o n s f o r i n s t a l l a t i o n a n d u s e •... -
Page 28: Sampling Positions
Figure 3-9: Sampling positions IMPORTANT! Air IDEAL 3P is delivered with a hook for suspending it in a glove box during decontamination phases. Even though sampling can be done while suspended, it is preferable to position Air IDEAL 3P along the axis of one-way flow at the air outlets. -
Page 29: Screwing On The Sampling Grid
Using Air IDEAL 3P Screwing on the sampling grid S c r e w i n g o n t h e s a m p l i n g g r i d CAUTION! Do not insert any foreign objects into the aspiration orifice located under the grid (see pages 3-6). -
Page 30: Electrical Power Supply
Audible signal each time the instrument is turned on: 2 long beeps CAUTION! During this battery low phase, Air IDEAL 3P remains in its normal operating condition until the sampling cycle in progress is finished. Before performing a new sampling cycle, recharge the battery. -
Page 31: Charging
Check that the available autonomy displayed is again 4 hours. IMPORTANT! If Air IDEAL 3P is not used for more than 10 days, the battery must be totally recharged (14 hours). O p e r a t i o n u s i n g t h e p o w e r l i n e Connection to the power line is carried out in the same way as for charging the internal battery. -
Page 32: Programming
Programming P r o g r a m m i n g Air IDEAL 3P enables samplings to be programmed and monitored. This section describes how to program the instrument. P r o g r a m m i n g c h a r a c t e r i s t i c s −... -
Page 33: Air Ideal 3P Menus
Using Air IDEAL 3P Programming Ai r I D E AL 3 P m e n u s Turning on WELCOME Last sample taken. Remaining autonomy. MESSAGE Selection of one of 4 sample volumes among the 4 volumes pre- MENU 1: programmed in menu 3. -
Page 34: Turning On Air Ideal 3P
Using Air IDEAL 3P Turning on Air IDEAL 3P T u r n i n g o n A i r I D E A L 3 P To turn on Air IDEAL 3P, • <ON/OFF>. Press The display indicates: Note: symbol is displayed if a delayed start-up is programmed. -
Page 35: Selection Of One Of The 4 Pre-Programmed Sample Volumes (Menu 1)
S e l e c t i o n o f o n e o f t h e 4 p r e - p r o g r a m m e d s a m p l e vo l u m e s ( M e n u 1 ) Factory programming memorizes the following 4 volumes in Air IDEAL 3P: 100, 500, 1000, 2000 liters. -
Page 36: Programming A Sample Volume Not In Memory (Menu 2)
Using Air IDEAL 3P Turning off Air IDEAL 3P P r o g r a m m i n g a s a m p l e vo l u m e n o t i n m e m o r y ( M e n u 2 ) To select menu 2, •... - Page 37 Using Air IDEAL 3P Turning off Air IDEAL 3P To program a new volume for program 1, • < > or < > Press until the desired volume is displayed. < > or < > program a new IMPORTANT! It is imperative to press the buttons immediately to volume.
- Page 38 Using Air IDEAL 3P Turning off Air IDEAL 3P To program a new volume for program 4, • < > or < > Press until the desired volume is displayed. < > or < > program a new IMPORTANT! It is imperative to press the buttons immediately to volume.
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Page 39: Programming Delayed Start-Up (Menu 4)
Using Air IDEAL 3P Turning off Air IDEAL 3P P r o g r a m m i n g d e l a y e d s t a r t - u p ( M e n u 4 ) Delayed start-up can be programmed for up to 60 minutes: −... -
Page 40: Programming Sequenced Sampling (Menu 5)
Using Air IDEAL 3P Turning off Air IDEAL 3P P r o g r a m m i n g s e q u e n c e d s a m p l i n g ( M e n u 5 ) Menu 5 is used to sample a selected volume several times. -
Page 41: Battery Autonomy Test (Menu 6)
The battery discharge cycle can be stopped as follows: • <STOP> Press To resume at a later time, • <START> Press CAUTION! If you wait more than 5 minutes, Air IDEAL 3P will turn off. ® Air IDEAL 3P User’s Manual 3-25... -
Page 42: Starting Sampling
S t a r t i n g s a m p l i n g CAUTION! Before starting, check that Air IDEAL 3P is fitted with a sampling grid and not its protective cover otherwise it could undergo irreversible damage which is not covered by the bioMérieux warranty. - Page 43 Note: The motor stops 2 liters before the total volume has been sampled. Last sampling not terminated: If Air IDEAL 3P was turned off during sampling, the following message appears the next time the instrument is turned on: CAUTION! In this case, the user will take all necessary precautions to deal with this interrupted sampling.
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Page 44: Stopping The Motor
If the motor is restarted after being stopped during a program, the program will resume from the point where it was stopped. Note: If stoppage during sampling is more than 5 minutes, Air IDEAL 3P will turn off. ® 3-28 Air IDEAL 3P User’s Manual... -
Page 45: Setting Air Ideal 3P Parameters (Summary Diagram)
Using Air IDEAL 3P Setting Air IDEAL 3P parameters (summary diagram) S e t t i n g A i r I D E A L 3 P p a r a m e t e r s ( s u m m a r y d i a g r a m ) - Page 46 Using Air IDEAL 3P Setting Air IDEAL 3P parameters (summary diagram) Menu 4 : Programming Menu 5 : Programming Menu 6 : Battery autonomy delayed start-up. sequences samplings. test Select delay before starting Press < > or < > until the Press <...
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Page 47: Procedure
P r o c e d u r e P r i n c i p l e Implementation and following of a procedure to measure aerobiocontamination corresponds to a process based on prevention, which involves: • Evaluating the current level and standard of hygiene and controls. •... -
Page 48: How To Obtain A Good Quality Sample
Procedure How to obtain a good quality sample H o w t o o b t a i n a g o o d q u a l i t y s a m p l e P r e c a u t i o n s o f u s e •... -
Page 49: Sampling Plan
Procedure Sampling plan S a m p l i n g p l a n The sampling plan must be drawn up very carefully and followed strictly. The aim is to guarantee that the values obtained are comparable. Any discrepancy between values should reveal a variation in aerobiocontamination. -
Page 51: Maintenance
S t e r i l i z a t i o n o f g r i d s Air IDEAL 3P is delivered with a set of 5 grids. They can be sterilized by autoclaving at 134°C for 18 min. -
Page 52: Decontamination In A Glove Box
D e c o n t a m i n a t i o n i n a g l o ve b o x Air IDEAL 3P was specially developed for use in production or control glove boxes. The suitability of Air IDEAL 3P for use in glove boxes was evaluated by the SKAN ag "Center of Competence for Isolator Technology". -
Page 53: Troubleshooting
Tr o u b l e s h o o t i n g Error message/signal Possible cause Recovery procedure REMOVE PROTECTION The protection cover was not removed. Install a grid. COVER ERROR The total duration of sequences and Repeat programming. intervals is too long. -
Page 55: Appendix
A p p e n d i x U s i n g t h e r e a d i n g t a b l e The following reading tables indicate the most probable number of microorganisms collected per plate (MPN collected) with respect to the number of agglomerates of colonies counted on the agar (CFU read). -
Page 56: Reading Tables
Appendix Reading tables The probability pi decreases as i increases. It is therefore particularly relevant to apply the statistical correction using FELLER’s law when high CFU values are read, i.e. close to N, i.e. in the case of agar plates almost completely saturated with colonies. - Page 57 Appendix Reading tables Corrected Corrected Corrected Corrected count count count count 209,340144 288,153475 400,832773 600,854201 211,548478 291,131003 405,401739 610,669016 213,775369 294,142367 410,050862 620,861324 216,021131 297,188344 414,783004 631,461324 218,286089 300,269739 419,601186 642,502990 220,570571 303,387386 424,508594 654,024729 222,874919 306,542148 429,508594 666,070184 225,199481 309,734919 434,604748 678,689231...
- Page 59 N o t e s ......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................® Air IDEAL 3P User’s Manual Notes-1...
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