bioMerieux VITEK 2 User Manual
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bioMerieux VITEK 2 User Manual

510731-4
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VITEK
2 User's Manual
510731-4
bioMérieux, Inc.
bioMérieux sa
Box 15969
69280 Marcy l'Etoile • France
Durham, North Carolina 27704-0969 USA
tel. 33 (0)4 78 87 20 00 / fax 33 (0)4 78 87 20 90
tel. (1) 800.682.2666
http://www.biomerieux.com

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Questions and answers

alkesh
January 18, 2025

kindly suggest the vitek 2 are available in user's privileges

0
Arka diagnostic
March 26, 2025

Jam condition

User image 67e3d70f04892
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1 comments:
Mr. Anderson
March 26, 2025

Jammed boats in the bioMérieux VITEK 2 occur when the Card Transport System encounters an obstruction. The system indicates jammed boats using dotted line rectangles on the Transport Halted screen.

### Causes:
1. Obstructions in the Card Transport System – Boats may get stuck due to mechanical issues or misalignment.
2. Incorrect Boat Positioning – If boats are not in their expected positions, a jam condition will occur.

### Solutions:
1. Identify the jammed boat on the Transport Halted screen.
2. Move the jammed boat to the major base pan position indicated by the animated dotted line rectangle and arrow.
3. Ensure the boat is moved exactly as shown on the screen to avoid recurring errors.
4. Close the front access door and cassette load/unload door.
5. Press "Go" to exit.

Failure to follow these steps correctly may result in a Card Transport System Failure error after rebooting.

This answer is automatically generated

0

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Summary of Contents for bioMerieux VITEK 2

  • Page 1 VITEK 2 User’s Manual 510731-4 bioMérieux, Inc. bioMérieux sa Box 15969 69280 Marcy l’Etoile • France Durham, North Carolina 27704-0969 USA tel. 33 (0)4 78 87 20 00 / fax 33 (0)4 78 87 20 90 tel. (1) 800.682.2666 http://www.biomerieux.com...
  • Page 2 33 (0)4 78 87 20 00 01-882 Warszawa Buenos Aires fax 33 (0)4 78 87 20 90 tel. (48) 22 663 44 44 tel. (54) 11 5555 6800 http://www.biomerieux.com fax (48) 22 669 99 42 fax (54) 11 5555 6888 Germany Portugal Australia bioMérieux Deutschland GmbH...
  • Page 3 This publication is based on software version VT2-R03.01 Updates are made periodically to this document. Software revisions and other changes will be included in subsequent versions. © Copyright 2002 by bioMérieux, Inc. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any human or computer lan- guage, in any form or by any means whatsoever without the express written permission of bioMérieux, Inc.
  • Page 5 WARRANTY Seller, bioMérieux, Inc., warrants the VITEK ® 2 instrument (the "instrument") to the original purchaser for a period of one (1) year after date of installation against defects in material and workmanship and defects arising from failure to conform to specifications applicable on the date of installation.

  • Page 7: Table Of Contents

    The SCS Keyboard ....... . 2-5 Starting the Smart Carrier Station..... 2-7 The VITEK 2 User’s Manual...
  • Page 8 Start-up Procedure ......4-8 What Happens Inside VITEK 2 ..... . 4-9 Test Card Processing Cycle .
  • Page 9 Screen Contrast ....... 5-17 How to Set VITEK 2 Configuration Options ... 5-18 Where to Find the Configuration Options .
  • Page 10 Where to Find It ....... 6-1 About the VITEK 2 Status Screen ....6-3 About the VITEK 2 Menu System .
  • Page 11 Where to Find It ....... 7-1 Maintaining the VITEK 2 Disposables....7-3 Monitoring the Disposables .
  • Page 12 Disposables Errors ......8-29 Working Without the VITEK 2 Bar Code Reader ..8-30 How to Enter Bar Codes Manually.

  • Page 13: List Of Figures

    PAGE 2-1 Initial SCS Screen ................. 2-8 5-1 The Instrument QC Status Screen ..........5-9 6-1 The VITEK 2 Status Screen in Cassette Only Mode ....6-4 6-2 Pipette Tips Status ................ 6-9 6-3 VITEK 2 Main Menu ..............6-10 6-4 Detailed Structure of the VITEK 2 Menu System ......

  • Page 15: General Warnings

    IMPORTANT: The user is advised to read and understand all instructions in this manual to be able to derive the best performance from VITEK 2. IMPORTANT: The configuration that you have purchased is adapted to the legislation and standards of the different countries it will be sent to.
  • Page 16 The VITEK 2 User’s Manual...
  • Page 17 DANGER: All organism suspensions should be consid- ered as potentially infectious. Qualified labo- ratory personnel should use acceptable procedures for biohazardous material The VITEK 2 User’s Manual xvii...

  • Page 18: Laser Caution Statement

    All access doors and covers must remain closed when process- ing cards to avoid exposure to laser light. Pages 4-6, 4-17, 8-41, and 8-49 xviii The VITEK 2 User’s Manual...

  • Page 19: Warning Messages

    CAUTION Statements All access doors should remain closed when processing cards. Page 4-6 The VITEK 2 test card transport system stops while the cassette load door is open. Be sure to close the door after loading or unloading a cassette.
  • Page 20 Page 6-34 Leaving the door to the Cassette Unload station open will inter- fere with the proper processing of the test cards in the VITEK 2 instrument. Page 6-56 Use aseptic technique when installing an accessory kit.
  • Page 21 The gloves that contain a powder can interfere with the optics. Page 8-43 The VITEK 2 instrument is heavy. Use at least two people to move it. Lift handles are provided at each end of the instrument. Page -1...

  • Page 22: Danger Statements

    Page 4-14 The sealer station contains a wire that is heated during the seal- ing operation. Do not reach into the instrument during the seal- ing operation. Page 4-23 xxii The VITEK 2 User’s Manual...
  • Page 23 Page 6-31 and 6-44 Do not attempt to load a cassette if a boat is not at the station. Damage to the cassette and the VITEK 2 instrument is possible. Page 6-33 and 6-46 All of the materials in an empty cassette should be treated as biohazards and disposed of accordingly.
  • Page 24 See 7-56 for instructions on cleaning the drip pan. Page 7-54 All organism suspensions should be considered as potentially infectious. Qualified laboratory personnel should use accept- able procedures for biohazardous material. Page 7-56 xxiv The VITEK 2 User’s Manual...
  • Page 25 Page 8-18 The instrument contains an ultraviolet light source. Make sure that the fluorescence optics unit is closed before resuming instrument operation. Page 8-43 The VITEK 2 User’s Manual...

  • Page 27: Standard Symbols

    Standard Symbols The symbols used in the VITEK 2 system are shown below: Direct current Alternating current Both direct and alternating current Three-phase alternating current Earth (ground) terminal Protective Conductor terminal Frame or chassis terminal Equipotentiality The VITEK 2 User’s Manual...
  • Page 28 On (only for part of equipment) Off (only for part of equipment) Equipment protected throughout by DOUBLE INSULATION or REINFORCED INSULATION (equivalent to Class II of IEC 536) Caution, risk of electric shock Caution Caution, elevated temperature xxviii The VITEK 2 User’s Manual...
  • Page 29 Smart Carrier Station Temperature Label ° Indicates a maximum ambient temperature of 30 C. The newer Smart Carrier Stations, beginning with Serial Number SCSA 3000, have a Maximum Temperature Label affixed to the Smart Carrier Station. The VITEK 2 User’s Manual xxix...

  • Page 31: How To Use This Manual

    Part 3, including only Chapter 6, is the primary part of the manual. It describes all of the procedures you need to follow to process VITEK 2 test cards. The chapter contains two subparts, one each to describe test card processing with, and without, a Smart Carrier Station.

  • Page 32: Printed Vs. Online Documentation

    This division allows for the possibility that the computer workstation may be in a different location than the VITEK 2 instrument and Smart Carrier Station. The VITEK 2 User’s Manual...

  • Page 33: How To Find Topics And Procedures

    The outside portion of the header shows the title of the current section. • Index. Found at the end of the manual, the index is the most useful device for finding individual topics throughout the manual. The VITEK 2 User’s Manual...

  • Page 34: Typographic Conventions

    The following typographic conventions are used throughout the manual: • Names of Keys. The names of keys, either on the VITEK 2 instrument keypad, or the Smart Carrier keyboard, are shown in capital letters, bold and italic. Example: Press the UP or DOWN ARROW keys.
  • Page 35 Cautions. A CAUTION statement is used when a particular action may affect the outcome of a test. • Danger. A DANGER statement is used when a particular action may result in personal injury, damage to the instru- ment, or create a biohazard. The VITEK 2 User’s Manual...

  • Page 37: The Smart Carrier Station

    What is the Smart Carrier Station? 2-2 • The SCS Base Unit 2-4 • The SCS Screen 2-5 • The SCS Bar Code Scanner 2-5 • The SCS Keyboard 2-5 • Starting the Smart Carrier Station 2-7 The VITEK 2 User’s Manual...

  • Page 38: What Is The Smart Carrier Station

    The Smart Carrier Station (SCS) is a small computer dedicated to collecting information about test cards and specimens, and for transferring that information to the VITEK 2 instrument. The SCS, shown in the figure below, has four main compo- nents: ❷...
  • Page 39 This memory chip stores the information you entered to be trans- ferred to the VITEK 2 instrument. ❷ The SCS Screen. The display for the SCS is a black and white liquid crystal display (LCD).

  • Page 40: The Scs Base Unit

    ❶ On/Off Switch ❷ Fuse Holder ❸ AC Power Receptacle ❹ 9-pin Service/Update Cable Connector ❺ RJ45 Receptacle for the Bar Code Scanner ❻ Circular Connector for the SCS Keyboard ❼ Contrast adjustment wheel for screen contrast The VITEK 2 User’s Manual...

  • Page 41: The Scs Screen

    The SCS Keyboard The Smart Carrier Station (SCS) keyboard has a standard set of alphabetic and numeric keys. It also has several keys that are uniquely designed for use on the SCS. The VITEK 2 User’s Manual...
  • Page 42 Press the Shift Lock key again to cancel upper case mode. For detailed descriptions of all the functions of the SCS key- board, see the table on page 6-19. The VITEK 2 User’s Manual...

  • Page 43: Starting The Smart Carrier Station

    AC power outlet. Connect the SCS keyboard to its receptacle (❷) on the back of the base unit. Connect the bar code scanner to its receptacle (❸) on the back of the base unit. The VITEK 2 User’s Manual...

  • Page 44: Initial Scs Screen

    NOTE: If you have not yet assigned a Station Name on the Configuration screen, the Configuration screen appears rather than the one above. For information on configuring the Station Name, see the instructions on page 3-5. The VITEK 2 User’s Manual...

  • Page 45: Configuring The Smart Carrier Station

    Why and How to Set the Options 3-4 – AST Dilution Mode 3-4 – Station Name 3-5 – Key Click 3-6 – Language 3-7 – Time and Date 3-8 – Firmware Update Options 3-9 – Cassette Erase 3-10 The VITEK 2 User’s Manual...

  • Page 46: Configuration Overview

    Key Click Language Time and Date Firmware Update Update all SCS files Options Update Specific file DensiChek Configu- DensiChek Connec- For future ration Cassette Erase Con- Erase Cassette 3-10 figuration Table 3-1: SCS Configuration Options The VITEK 2 User’s Manual...

  • Page 47: When Should Configuration Options Be Set

    SCS for which a Station Name has not been defined. NOTE: One exception to this is the Program Updates option. You do not need to use that screen until you receive instructions from bioMérieux to do so. The VITEK 2 User’s Manual...

  • Page 48: How Do You Access The Configuration Screens

    If you set the AST Dilution Mode to automatic, every AST in a cassette has its inoculum diluted by VITEK 2. If the AST Dilution Mode is set to pre-diluted, VITEK 2 assumes that the AST dilutions have already been performed.

  • Page 49: Station Name

    SCS, that individual’s initials can be used for the SCS name. The station’s name is stored in the cassette’s button memory. The name is transferred to the VITEK 2 instrument and is then sent to the computer. The station name becomes a part of each test card’s address that appears on the card directory.

  • Page 50: Key Click

    In the Key Click field, press 1 to turn the key click off, 2 to turn the key click on. Press the F2 key to exit the SCS Configuration screen or the NEXT or PREVIOUS SCREEN keys to access other configuration screens. The VITEK 2 User’s Manual...

  • Page 51: Language

    Press the UP or DOWN ARROW keys to select a language, and then press ENTER. Press the F2 key to exit the SCS Configuration screen or the NEXT or PREVIOUS SCREEN keys to access other configuration screens. The VITEK 2 User’s Manual...

  • Page 52: Time And Date

    Press the ENTER key to accept the new value. Enter the current minutes. Press the ENTER key to accept the new value. Enter the current day. Press the ENTER key to accept the new value. Enter the current month. The VITEK 2 User’s Manual...

  • Page 53: Firmware Update Options

    SCS files are included in the workstation. NOTE: Make sure the SCS is connected to the workstation. Press the F4 key to access the Configuration screen. Press the NEXT or PREVIOUS SCREEN key until the Firmware Update Options screen displays. The VITEK 2 User’s Manual...

  • Page 54: Cassette Erase

    Cassette Erase The Cassette Erase function gives you the option of automati- cally erasing data in the button memory of cassettes that have been processed by the VITEK 2. OW TO PTION Press the F4 key to access the Configuration screen.
  • Page 55 Smart Carrier Station, the following message displays: Cassette has been processed. Press F1 to erase, any other key to display processed information. Press F2 to exit the screen. The VITEK 2 User’s Manual 3-11...

  • Page 57: The Vitek 2 Instrument

    4. The VITEK 2 Instrument Introduction This chapter describes the hardware systems in the VITEK 2 instrument. The section External Components of the VITEK 2 Instrument points out the controls and connections found on the instrument’s exterior, and the doors by which you gain access to its interior.
  • Page 58 The VITEK 2 Instrument – The Sealer Station 4-23 – Test Card Incubation and Reading 4-23 – Card Ejection 4-26 – The Waste Collection Station 4-28 • The User Interface System 4-29 – Keypad and Screen 4-30 The VITEK 2 User’s Manual...

  • Page 59: External Components Of The Vitek 2 Instrument

    External Components of the VITEK 2 Instrument The picture on the following page contains a front and a left side view of the VITEK 2 and VITEK 2 XL instrument. Use the diagram to locate and familiarize yourself with the external controls and the access doors to the interior of the instrument.
  • Page 60 External Components of the VITEK 2 Instrument The VITEK 2 Instrument ❾ ❺ ❶ ❹ ❷ ❸ ➑ VITEK 2 60 Instrument ❾ ❻ ❼ ❹ ❶ ❹ ❷ ❿ ❸ ➑ VITEK2 XL Instrument The VITEK 2 User’s Manual...

  • Page 61: Controls And Connections

    The VITEK 2 Instrument External Components of the VITEK 2 Instrument Controls and Connections ❶ User Interface Screen and Keypad. This screen and key- pad comprise the User Interface system. See page 4-29 for detailed information. ❷ Front access door. Provides access to the diluter, pipette tip container, and a portion of the test card transport sys- tem.

  • Page 62: Access Doors

    NOTE: This door is secured by two screws, and requires a flathead screwdriver to open. ❻ Top access door Section A. VITEK 2 XL only. Provides access to the optics and carousel for Section A. ❼ Top access door Section B. VITEK 2 XL only. Provides access to the optics and carousel for Section B.
  • Page 63 The VITEK 2 Instrument External Components of the VITEK 2 Instrument ❿ Center front sliding door. VITEK 2 XL only. The Cassette load/unload door (❸) must be opened first before this door can be opened. 11 UPS connection. This cable connector port connects VITEK 2 to an uninterruptable power supply (UPS).

  • Page 64: Turning On The Vitek 2 Instrument

    13 in the figure on page 4-4.) The instrument goes through an initialization sequence that includes several self tests. During this time, VITEK 2 is also bringing the carousel area up to its specified temperature for test card incubation.

  • Page 65: What Happens Inside Vitek 2

    Test Card Processing Cycle The parts and functions of the VITEK 2 instrument can best be described by following a test card through the phases of a typi- cal processing cycle. The following table summarizes this cycle, and shows you where you can find more details about a particular component of the VITEK 2 instrument.

  • Page 66: The Smart Carrier Station (Scs)

    The VITEK 2 Instrument The Smart Carrier Station (SCS) The SCS is not a part of the VITEK 2 instrument, but it is the station where the entire process begins. For complete details on the SCS, see Chapter 2, The Smart Carrier Station.
  • Page 67 Station, the button memory stores the information that you enter for each test card. This information is read by a station in VITEK 2, which marks the memory chip as being read, allowing the cassette to be reused. The VITEK 2 User’s Manual...
  • Page 68 Fit into a boat. The shape of the cassette base matches the well on the top of a boat. This ensures that the two units move as one through VITEK 2. The shape also ensures that the cassette can only be put into a boat in the proper orientation.

  • Page 69: Cassette Load And Unload Station

    What Happens Inside VITEK 2 Cassette Load and Unload Station You load and unload cassettes from VITEK 2 using this station. As seen in the picture, the station consists of the cassette load door and a green indicator light. The door has a locking mecha- nism.
  • Page 70 What Happens Inside VITEK 2 The VITEK 2 Instrument CAUTION: The VITEK 2 test card transport system stops while the cas- sette load door is open. Be sure to close the door after load- ing or unloading a cassette. DANGER: The boat should be considered as potentially contaminated and should be handled appropriately.

  • Page 71: The Boats

    ❷ The base of each boat is notched in several places. These areas are used by the VITEK 2 test card trans- port mechanisms that move the boats through the module.

  • Page 72: Vitek 2 Bar Code Reader

    After a cassette has been loaded onto a boat, the test card trans- port system moves the boat past the bar code reader station. This station reads the information encoded on the bar code label found on each VITEK 2 test card. The following information is included in the bar code: •...

  • Page 73: The Button Memory Reader

    The Button Memory Reader is a device that VITEK 2 uses to read the test card information stored by the Smart Carrier Sta- tion in the memory chip on a cassette. VITEK 2 uses this test card information in two ways: •...

  • Page 74: Vitek 2 Dispenser/Pipettor Station

    ❶ ❷ ❹ ➌ Sterile Saline. The top of VITEK 2 has a compartment designed to hold a one-liter bag of sterile saline (❶) . This is enough saline to process about 330 AST cards. 4-18 The VITEK 2 User’s Manual...
  • Page 75 The VITEK 2 Instrument What Happens Inside VITEK 2 Dispenser Assembly. The dispenser assembly consists of a plastic dispensing chamber and two sections of plastic tubing. One section of tubing (➋) leads from the dispens- ing chamber and is attached to the sterile saline bag. The other section of tubing (➌) also leads from the dispensing...
  • Page 76 What Happens Inside VITEK 2 The VITEK 2 Instrument IPETTOR The pipettor station transfers a ❶ preset volume of an organism suspension from its test tube ❷ into the test tube of its mated AST card. ❶ Disposable Pipette Tips.
  • Page 77 The VITEK 2 Instrument What Happens Inside VITEK 2 OW THE ISPLACEMENT ORKS The displacement pump is part of a larger assembly that per- forms the pipetting and transfer of the suspension when VITEK 2 is in the Automatic dilution mode. Here is how it works: 1.

  • Page 78: The Filler Station

    Various temperature and air pressure sensors in the system monitor the inside of the vacuum chamber. VITEK 2 ensures proper test card fills by monitoring these parameters throughout the entire cycle and controlling the rate at which the vacuum is drawn and released.

  • Page 79: The Sealer Station

    Do not reach into the instrument during the sealing operation. The Sealer station completes the functions inside VITEK 2 that prepare the test cards for incubation and reading. This is accom- plished by heat-sealing the transfer tube that delivered inocu- lum to the test card from its test tube.
  • Page 80 What Happens Inside VITEK 2 The VITEK 2 Instrument AROUSEL The Carousel, pictured here, has a capacity of 60 test cards. During their time in the carousel, the test cards are incubated at an average temperature of 35.5°C. the carousel rotates, each test card moves into the reading position every 15 minutes.
  • Page 81 The VITEK 2 Instrument What Happens Inside VITEK 2 PTICS VITEK 2 performs its identification and susceptibility analyses by continually monitoring the growth and activity of organisms inside the wells of the test cards. Two different optics systems perform ths function:...

  • Page 82: Card Ejection

    What Happens Inside VITEK 2 The VITEK 2 Instrument RANSMITTANCE PTICS The transmittance optics use visible light to directly measure organism growth. These optics are based on an initial light reading of a well before significant growth has begun. Light...
  • Page 83 Configuration window at the workstation computer. Cards can also be ejected at any time using a manual ejection function. CAUTION: Ejected test cards must not be reinserted into VITEK 2. Make sure that all test card processing has been completed before ejecting a test card.

  • Page 84: The Waste Collection Station

    VITEK 2 interface screen when the station is full. A sensor in the station detects when the tray has been emptied or if the tray is missing.

  • Page 85: The User Interface System

    See Cleaning the Test Card Collection Tray on page 7-49. The User Interface System Throughout the entire processing cycle for test cards, communi- cation between the user and VITEK 2 is essential. The User Interface System of VITEK 2 provides the means of that com- munication.

  • Page 86: Keypad And Screen

    The VITEK 2 Instrument Keypad and Screen A keypad and screen are located on the front of VITEK 2. VITEK 2 uses the screen to send you messages about its opera- tion, on-board disposables, and possible problems. You use the keypad to respond to VITEK 2 instructions, send commands to VITEK 2, and to perform other functions.
  • Page 87 ❻ ENTER Key. Use this key to complete data entries, or when instructed to do so on a screen. ❼ NUMERIC Keys. Use these keys to enter a number onto a screen. The VITEK 2 User’s Manual 4-31...

  • Page 89: Configuring The Vitek 2 Instrument

    5. Configuring the VITEK 2 Instrument Introduction Configuration options allow you to operate the VITEK 2 instru- ment in a number of different ways. This chapter explains not only how to set these options, but how each option affects the operation of the instrument, and therefore your laboratory work flow.
  • Page 90 Introduction Configuring the VITEK 2 Instrument • How to Set VITEK 2 Configuration Options 5-18 – Where to Find Configuration Options 5-19 – How to Define Character Sets 5-20 – How to Set Time for the QC Status 5-22 – How to Use Option Boxes 5-24 –...

  • Page 91: Configuration Overview

    Configuration Overview Configuration Overview What are the Configuration Options? There are 11 configuration options in the VITEK 2 user inter- face. They can be divided into two groups, depending on whether or not the option affects how the instrument operates.

  • Page 92: When Should Configuration Options Be Set

    Configuration Overview Configuring the VITEK 2 Instrument The group of options that only affect the physical parameters of the user interface is composed of the following options: Find a Option Description on Page… Audible Alarm Enable 5-13 Audible Alarm Volume...

  • Page 93: How Configuration Options Affect Vitek 2

    VITEK 2 users. When a cassette is processed, the Cassette Name becomes part of what is called the cassette address. When viewing test card directories on the VITEK 2 workstation, the cassette address can be used to filter the direc- tory view so that only those test cards processed by a particular user are seen.
  • Page 94 SCS, or if you have a work flow that does not require the use of an SCS. NOTE: If the VITEK 2 instrument is in Smart Carrier mode, the Cassette Names option cannot be accessed. If you...

  • Page 95: Instrument Name

    Configuring the VITEK 2 Instrument How Configuration Options Affect VITEK 2 Instrument Name The Instrument Name option allows you to name a VITEK 2 instrument module. The name can contain up to 20 printable characters, including spaces. The instrument name can be help-...

  • Page 96: Schedule Instrument Qc Status

    The QC Status is a report of the current incubator temperature and the status of the optics systems. The report is sent to the VITEK 2 workstation where it is recorded with the date and time the report was taken.

  • Page 97: The Instrument Qc Status Screen

    The field is blank if this function has never been used before. ❷ Temperature. For the VITEK 2, this field displays the cur- rent carousel temperature in degrees Celsius. For the VITEK 2 XL, the temperatures for carousel A and B are displayed.

  • Page 98: Cassette Mode

    The procedure for setting this option is How to Set Time for the QC Status on page 5-22. Cassette Mode The Cassette Mode option tells the VITEK 2 instrument whether or not you use a Smart Carrier Station (SCS), and is critical to instrument operation. The option enables or disables the instrument’s Button Memory Reader, a device that reads...

  • Page 99: Dilution Mode

    Dispenser/Pipettor station is determined by the information in the cassette’s button memory. NOTE: If the VITEK 2 instrument is in Smart Carrier mode, the Dilution Mode option cannot be accessed. If you attempt to use the option, the screen displays the mes- sage.

  • Page 100: Bar Code Reader

    Use Option Boxes on page 5-24. Bar Code Reader The VITEK 2 instrument has a bar code station that reads the bar code labels on the test cards. If the reader becomes mis- aligned, or if some other error occurs in the station, you could get repeated error messages about bad bar codes.

  • Page 101: Audible Alarm Enable

    Configuring the VITEK 2 Instrument How Configuration Options Affect VITEK 2 Audible Alarm Enable The instrument sounds an audible alarm to alert you to an error condition. The alarm stops temporarily when you press any key on the instrument keypad, sounding again if you do not access the message queue.

  • Page 102: Audible Alarm Volume

    How Configuration Options Affect VITEK 2 Configuring the VITEK 2 Instrument Audible Alarm Volume The volume of the audible alarm can be adjusted higher or lower to account for laboratory conditions. SE THIS PTION The default setting for the alarm volume is at the midpoint of its range.

  • Page 103: Visual Alarm Enable

    The blinking terminates when you press any key on the instrument keypad. SE THIS PTION Since the VITEK 2 instrument and workstation each have an audible alarm, the visible alarm may be unnecessary. If so, use this option to Disable the alarm. CAUTION: Do not disable both the audible and visual alarms on the instrument unless it is located very near to the workstation.

  • Page 104: Keyclick Volume

    Configuring the VITEK 2 Instrument Keyclick Volume The VITEK 2 instrument interface uses a “touch pad” type of keypad. It sounds an audible click when you press each key. The volume of the click can be adjusted to account for labora- tory conditions.

  • Page 105: Screen Contrast

    Configuring the VITEK 2 Instrument How Configuration Options Affect VITEK 2 Screen Contrast The VITEK 2 user interface screen uses an LCD display. This option controls the amount of background contrast on the dis- play. SE THIS PTION Viewing of an LCD screen can be enhanced by changing the contrast under certain lighting conditions.

  • Page 106: How To Set Vitek 2 Configuration Options

    How to Set VITEK 2 Configuration Options Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options There are eleven configuration options, but only four different procedures for setting them, depending on the interface used. The four interfaces used and the applicable configuration...

  • Page 107: Where To Find The Configuration Options

    Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options Where to Find the Configuration Options All of the configuration options for VITEK 2 are on the Config- uration Menu. You access this menu using the path: VITEK 2 Main Menu ⇒...

  • Page 108: How To Define Character Sets

    How to Set VITEK 2 Configuration Options Configuring the VITEK 2 Instrument How to Define Character Sets Use this procedure for the Cassette Names and Instrument Name configuration options. Character sets can include any of the characters provided on the character selection, plus the 10 digits from the keypad.
  • Page 109 Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options The character selection screen appears: Cassette Names Clear A B C D E F G H I J K L M Space N O P Q R S T U V W X Y Z...

  • Page 110: How To Set Time For The Qc Status

    How to Set VITEK 2 Configuration Options Configuring the VITEK 2 Instrument How to Set Time for the QC Status The function allows you to set up to three times during the day at which an instrument QC report is sent to the workstation.
  • Page 111 Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options To set the Hour for Time A: Press the LEFT ARROW key until ‘Hours’ displays. Use the UP or DOWN ARROW keys to set the Hour. To set the Minutes for Time A: Press the LEFT ARROW key until ‘Minutes’...

  • Page 112: How To Use Option Boxes

    How to Set VITEK 2 Configuration Options Configuring the VITEK 2 Instrument How to Use Option Boxes This procedure applies to the following configuration options: • Cassette Mode • Dilution Mode • Bar Code Reader • Audible Alarm Enable •...

  • Page 113: How To Set A Range Value

    Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options How to Set a Range Value This procedure applies to the following configuration options: • Audible Alarm Volume • Keyclick Volume • Screen Contrast Access one of the above configuration options using...
  • Page 114 How to Set VITEK 2 Configuration Options Configuring the VITEK 2 Instrument Below is an example of a range value screen. Screen Contrast Press the DOWN ARROW to view the configuration options. A range bar appears: Change the range value by pressing the LEFT or RIGHT ARROW keys.
  • Page 115 Configuring the VITEK 2 Instrument How to Set VITEK 2 Configuration Options NOTE: Do NOT set the screen contrast to either end of its available range. Doing so may make the screen unus- able. NOTE: For the Audible Alarm Volume parameter, a test but- ton is provided to sound the alarm.

  • Page 117: Processing Vitek 2 Test Cards

    VITEK 2 test cards. The chapter begins with three introductory sections that provide reference information about the VITEK 2 Status screen, the VITEK 2 Menu system, and the Smart Carrier Station interface. The next two sections contain suggested work flows and the procedures for working either with or without a Smart Carrier Station.
  • Page 118 Introduction Processing VITEK 2 Test Cards • Processing Test Cards Using the Smart Carrier Station 6-25 – Configuration Options to Consider 6-25 – The Work Flow 6-26 – How to Enter Data With the SCS 6-28 – Loading a Cassette 6-31 •...

  • Page 119: About The Vitek 2 Status Screen

    About the VITEK 2 Status Screen You use the Status screen more often than any other screen in the VITEK 2 user interface. Since it is so basic to the work flow, the Status screen is very easy to display and in many cases, the interface displays it automatically.

  • Page 120: The Vitek 2 Status Screen In Cassette Only Mode

    Introduction Processing VITEK 2 Test Cards NSTRUMENT TATUS IELD The Status field appears at the top of the VITEK 2 Status screen. 12:00 ❶ Status: OK ❷ MAX Available Slots: 60 ❸ Cassette Name: NSB ❹ Dilution Mode: Automatic ❺...
  • Page 121 Processing VITEK 2 Test Cards Introduction • Messages. This status indicates that there is an error mes- sage in the error/message queue that has not been viewed. Press the Message key (?) to view the error/message queue. • Errors. This status appears after the error/message queue is accessed if the error condition has not been resolved.
  • Page 122 Introduction Processing VITEK 2 Test Cards The number you see in the MAX Available Slots field depends on the VITEK 2 instrument you have and the optical configura- tion of the instrument. Instrument/Max Available Optical Configuration VITEK 2 • 1 fluorescence optic MAX Available Slots: 60 •...
  • Page 123 Automatic or Pre-Diluted dilution mode. This field should be checked before loading a cassette. DANGER: Biohazardous spills can occur inside the VITEK 2 instru- ment if the Dilution Mode option is not properly set, espe- cially if pre-diluted samples are used with the mode set to Automatic.
  • Page 124 Status screen (❺) icons. • VITEK 2 Instrument Icon. This icon represents the VITEK 2 instrument. It shows when one of the access doors is open. These include the front access door on the front of the instrument, and the top access door.

  • Page 125: Pipette Tips Status

    IPETTE IPS AND ALINE The VITEK 2 instrument uses pipette tips and saline (❻) for automatic dilution of susceptibility inocula. For each disposable the Status screen displays a bar graph and number similar to the one shown in Figure 6-2:...

  • Page 126: About The Vitek 2 Menu System

    Processing VITEK 2 Test Cards About the VITEK 2 Menu System All of the functions used on the VITEK 2 instrument are avail- able through the menu system. The system is composed of a Main Menu and a set of five submenus.
  • Page 127 Processing VITEK 2 Test Cards Introduction The VITEK 2 User’s Manual 6-11...

  • Page 128: The Previous Screen Key

    Maintenance FTEN There are several keys on the keypad that are used often when using the VITEK 2 menu system. • Option Buttons. There are five round buttons located along the right side of the user interface screen. When a menu or function option appears on the screen, you press the corre- sponding option button to access that menu or function.

  • Page 129: About The Smart Carrier Station

    Processing VITEK 2 Test Cards About the Smart Carrier Station If a menu displays on the screen, pressing the PREVI- OUS SCREEN key returns the display to the previous menu. If the Main menu displays on the screen, pressing the PREVIOUS SCREEN key returns the display to the Sta- tus screen.
  • Page 130 A matched ID/AST card set provides two advantages: In Automatic Dilution mode, the inoculum for the AST card is created automatically in the VITEK 2 instru- ment. The organism identification called by the ID card is automatically applied to its matching AST card.
  • Page 131 Safety. The cassette portion of the SCS provides a safe and convenient method of transporting multiple test cards from the workbench to VITEK 2. Once a sample is placed in the cassette, the technologist never has to touch it again.

  • Page 132: Data Fields

    About the Smart Carrier Station Processing VITEK 2 Test Cards Data Fields There is one data entry screen with six fields. The fields are repeated for each of the available 15 slots in the cassette. Station name is: TRB Slot: 6...
  • Page 133 Processing VITEK 2 Test Cards About the Smart Carrier Station Field Description ❺ Ancillary This field holds the results of off-line tests Tests performed for some test cards. ❻ Modifier This field is used for organism modifiers. Six generic entries, mod1 to mod6, are preloaded.
  • Page 134 About the Smart Carrier Station Processing VITEK 2 Test Cards Field Description ➑Slot Indicator The slot indicator is the set of boxes at the bottom of the Data Entry screen (see call- out (❽) in Figure 6-6 on page 6-16). The...

  • Page 135: Using The Slot Indicator

    Processing VITEK 2 Test Cards About the Smart Carrier Station Using the Slot Indicator • A filled-in circle (●) appears under a box when the SCS determines the test card in the slot requires an organism sus- pension. A completely open circle (❍) appears under a box when the test card in the slot requires an empty tube.
  • Page 136 About the Smart Carrier Station Processing VITEK 2 Test Cards Use these To… Do this: keys… Press the NEXT or PREVIOUS Display an adjacent SLOT key. slot: Information must have been Copy data to the cur- entered in a previous slot.
  • Page 137 Processing VITEK 2 Test Cards About the Smart Carrier Station Use these To… Do this: keys… Press the F9 key. A confirma- Erase the data for a tion window appears. slot. Press the F1 key to erase the NOTE: This func- tion also erases data data.

  • Page 138: Option Boxes

    About the Smart Carrier Station Processing VITEK 2 Test Cards Option Boxes The Organism ID, Ancillary Test, and Modifier fields are restricted, that is, they accept only certain data. When entering data manually via the keyboard, option boxes provide access to the acceptable data entries.
  • Page 139 Processing VITEK 2 Test Cards About the Smart Carrier Station ODIFIER The option box for the Modifier field contains six default entries, “mod 1” to “mod 6.” When this data reaches the work- station computer, the entry is matched to one of the first six entries in the Organism Modifier table of the bioLIAISON data- base.

  • Page 140: About The Bar Code Scanner

    Bar code labels on every test card allow you to scan in the entry for the Card Type Field. Additional information from this bar code is also stored in the button memory of the cas- sette. This information helps VITEK 2 properly identify and process each test. 6-24...

  • Page 141: Processing Test Cards Using The Smart Carrier Station

    See AST Dilution Mode on page 3-4. • On the VITEK 2 Instrument. The most important config- uration option for using the SCS is the Cassette Mode option. This option must be set to Smart Carrier.

  • Page 142: The Work Flow

    The following table provides a general work flow for using the Smart Carrier Station (SCS). This work flow assumes that you have • selected the VITEK 2 test cards to process • decided whether to prepare susceptibility suspensions man- ually or automatically using VITEK 2...
  • Page 143 Processing VITEK 2 Test Cards Processing Test Cards Using the Smart Carrier Station Activity For more information see… C. Select a slot in the • Using the Slot Indicator on cassette for the first page 6-19 test card and speci- men.

  • Page 144: How To Enter Data With The Scs

    Processing Test Cards Using the Smart Carrier Station Processing VITEK 2 Test Cards How to Enter Data With the SCS The Data Entry screen is used to store information about speci- mens and their test cards. You enter the information by posi- tioning the cursor in a data field for a particular test card slot.
  • Page 145 Processing VITEK 2 Test Cards Processing Test Cards Using the Smart Carrier Station Enter the data: Using the bar code Field Using the keyboard… scanner… Accession ID Scan the bar code Type the ID into the Acces- label on the speci- sion ID field.
  • Page 146 If you pre-dilute the inocula for the susceptibility tests, you can place the test cards into any slots in the cassette, in any order you choose. However, when VITEK 2 is performing the dilu- tion (Automatic dilution mode), you must follow these rules: •...

  • Page 147: Loading A Cassette

    After the information for a cassette has been entered at the SCS, the cassette is ready to be loaded into the VITEK 2 instrument. Before you load the cassette, however, you should always check the Status screen. Look at the following parameters to...
  • Page 148 Processing Test Cards Using the Smart Carrier Station Processing VITEK 2 Test Cards SING THE ASSETTE TATION The Cassette Load station is composed of the cassette load door and a green indicator light. The indicator light tells you the sta-...
  • Page 149 DANGER: Do not attempt to load a cassette if a boat is not at the sta- tion. Damage to the cassette and the VITEK 2 instrument is possible. Place the cassette in the boat so that the specimen test tubes face the front of the instrument.
  • Page 150 After a cassette is loaded, the test cards in it are scanned by the bar code reader in VITEK 2. This information is sent to the workstation so that you can see a listing of the test cards in the Directory window.

  • Page 151: The Cassette Address

    Processing Test Cards Using the Smart Carrier Station BOUT THE ASSETTE DDRESS The cassette address, which appears in the VITEK 2 Directory screen at the workstation, provides a means to identify each test card in the VITEK 2 instrument. The following figure illustrates two examples:...

  • Page 152: Processing Test Cards In Cassette Only Mode

    NOTE: If your system has a Smart Carrier Station, refer to the section Processing Test Cards Using the Smart Car- rier Station on page 6-25. Configuration Options to Consider On the VITEK 2 instrument, set the • Cassette Mode option to Cassette Only. •...

  • Page 153: The Work Flow

    The following table provides a generalized work flow for work- ing without a Smart Carrier Station (SCS). This work flow assumes that you have • selected the VITEK 2 test cards to process. • decided whether to prepare susceptibility suspensions man- ually or automatically using VITEK 2.
  • Page 154 6-42. or enter a cassette name, and, if necessary, change the dilution mode. F. Load the cassette onto the VITEK 2 Loading a Cassette on page 6-44. instrument. G. Enter the information from the cas- Monitoring Test Card Processing on sette worksheet into the Cassette page 6-47.

  • Page 155: Cassette Preparation

    OW TO ENERATE A ASSETTE ORKSHEET You generate Cassette worksheets at the VITEK 2 workstation. For information, please refer to this topic on the VITEK 2 Online Help System. OW TO SE THE ASSETTE ORKSHEET The Cassette Worksheet, shown in Figure 6-8, is designed to help you organize a set of test cards and specimens for a cas- sette.

  • Page 156: The Cassette Worksheet

    Fill in the cassette name here. ❷Tests Fill in the number of test cards being placed in the cassette. ❸Instrument Name Fill in the name of the VITEK 2 instrument into which you placed the cassette. 6-40 The VITEK 2 User’s Manual...
  • Page 157 If you pre-dilute the inocula for the susceptibility tests, you can place the test cards into any slots in the cassette, in any order you choose. However, when VITEK 2 is performing the dilu- tion (Automatic dilution mode), you must follow these rules: •...

  • Page 158: Cassette Setup Screen

    Access the Cassette Setup function on the Main Menu. NOTE: If the VITEK 2 instrument is in Smart Carrier mode, the Cassette Setup function cannot be accessed. If you attempt to use the option, the screen displays the mes-...
  • Page 159 (❸) to change the current dilution mode (❷). DANGER: Biohazardous spills can occur inside the VITEK 2 instru- ment if the Dilution Mode option is not properly set, espe- cially if pre-diluted samples are used with the mode set to Automatic.

  • Page 160: Loading A Cassette

    Processing Test Cards in Cassette Only Mode Processing VITEK 2 Test Cards Loading a Cassette DANGER: The cassette should be considered as potentially contami- nated and should be handled appropriately. Qualified labo- ratory personnel should take the usual precautions necessary for infectious agents.
  • Page 161 Processing VITEK 2 Test Cards Processing Test Cards in Cassette Only Mode SING THE ASSETTE TATION The Cassette Load station shown below is composed of the cas- sette load door and a green indicator light. The indicator light, shown in the figure above, tells you the sta-...
  • Page 162 DANGER: Do not attempt to load a cassette if a boat is not at the sta- tion. Damage to the cassette and the VITEK 2 instrument is possible. Place the cassette in the boat so that the specimen test tubes are towards the front of the instrument.
  • Page 163 After a cassette is loaded, the test cards in it are scanned by the bar code reader in VITEK 2. This information is sent to the workstation so that you can see a listing of the test cards in the Directory window.

  • Page 164: How To Enter Data For A Cassette

    The information can be viewed in the Cassette Edit window, which is part of the VITEK 2 workstation software. You can access that window and, using the Cassette Worksheet, fill in the remaining information for the cassette once the test cards have been loaded into the carousel.

  • Page 165: The Cassette Address

    DDRESS The cassette address, which appears in the VITEK 2 Directory window at the workstation, provides a means to identify each test card in the VITEK 2 instrument. The following figure illus- trates two examples: RMT-03 Apr 03 08:35:40 URN-11 Mar 29 13:45:01 Figure 6-10: The Cassette Address A cassette address has three basic parts.

  • Page 166: Batch Loads (Smart Carrier Or Cassette Only Mode)

    Batches of three or four cassettes can be loaded in rapid succes- sion using the Batch Load function. EFORE EGIN Because a batch load requires most or all of VITEK 2’s resources, you should do the following before initiating a batch load: Batch loading requires at least three cassettes, so check your work load to make sure that you are pro- cessing enough test cards.

  • Page 167: To Begin A Batch Load

    NOTE: You must prepare all three or four of the cassettes to be included in the batch load before proceeding. Bring the cassettes to the VITEK 2 instrument. Access the Batch Load function on the Main Menu. The VITEK 2 User’s Manual...
  • Page 168 Batch Loads (Smart Carrier or Cassette Only Mode) Processing VITEK 2 Test Cards The VITEK 2 instrument checks to ensure that at least three boats are available, and that there are no test cards currently in the card transport system. It then displays the...
  • Page 169 Processing VITEK 2 Test Cards Batch Loads (Smart Carrier or Cassette Only Mode) The following screen displays for Smart Carrier Mode: Batch Load Load cassette number 1 Abort this cassette NOTE: The following screen displays for Cassette Only Mode: Batch Load...

  • Page 170: Unloading The Cassette And Removing Waste

    Unloading the Cassette and Removing Waste Processing VITEK 2 Test Cards The instrument moves the boat away from the load sta- tion, moves the next boat into the station, and redisplays the screen shown in the previous step. Repeat Step 4 as required for the other cassettes.

  • Page 171: How To Unload A Cassette

    Processing VITEK 2 Test Cards Unloading the Cassette and Removing Waste How to Unload a Cassette During test card processing, test cards are unloaded from the cassette and placed into the carousel at the Card Reader and Incubator station. This means that when the boat and cassette return to the unload station, only the specimen test tubes and the severed transfer tubes remain in the cassette.
  • Page 172 Unloading the Cassette and Removing Waste Processing VITEK 2 Test Cards CAUTION: Leaving the door to the Cassette Unload station open will interfere with the proper processing of the test cards in the VITEK 2 instrument. Dispose of the materials in the cassette. The cas- sette can now be used to process additional test cards.

  • Page 173: How To Remove Ejected Test Cards

    Processing VITEK 2 Test Cards Unloading the Cassette and Removing Waste How to Remove Ejected Test Cards When a test card is ejected, it is removed from the carousel in the Card Reader and Incubator station, and placed in the Waste Collection station (see Figure 6-11).
  • Page 174 NOTE: Once cards have been removed from the Waste Collec- tion station, never reinsert them. This will cause the instrument to jam. DANGER: Although VITEK 2 test cards are sealed, they should be treated as biohazards and disposed of accordingly. EPLACING THE ASTE...

  • Page 175: Introduction

    7. Maintaining the VITEK 2 Instrument Introduction This chapter includes the procedures required to maintain the VITEK 2 instrument. Maintenance includes monitoring and changing the disposables, cleaning the boats and the carousel, and general maintenance and cleaning. NOTE: The Maintenance Log provided at the end of this chapter lists recommended maintenance procedures and their frequency.
  • Page 176 – How to Clean the Boats 7-42 – How to Replace Boats after Cleaning 7-42 • Cleaning the Inside of the VITEK 2 Instrument 7-46 – Shutting Down the VITEK 2 Instrument 7-46 – Cleaning the Test Card Collection Tray 7-49 –...

  • Page 177: Maintaining The Vitek 2 Disposables

    Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Disposables Chapter 6, Processing VITEK 2 Test Cards, introduced the Sta- tus screen and how to incorporate it into your work flow. A spe- cific part of that screen is devoted to the maintenance of the...
  • Page 178 This means that the graphs and values are important only when you are processing ASTs in the Automatic Dilution mode, that is, when the VITEK 2 instrument is performing the dilution of the AST inoculums. For example, when one of the graphs is at one-half capacity, it means that enough of that disposable remains to process approximately 160 ASTs.

  • Page 179: The Dispenser/Pipettor Accessory Kit

    XPIRATION OF ISPOSABLES VITEK 2 is programmed to measure the amount of time that the disposables have been in the instrument. When the specified time limit is exceeded, VITEK 2 displays an error message tell- ing you that a disposable has expired.

  • Page 180: How To Install An Accessory Kit, Part A: Saline And Tubing

    Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument How to Install an Accessory Kit, Part A: Saline and Tubing NOTE: If the card transport system is processing cassettes through the card transport station, the following mes- sage appears: Card transport system is busy.
  • Page 181 Maintaining the VITEK 2 Instrument Maintaining the VITEK 2 Disposables Press the Yes option button to continue with the saline change. After you proceed the instrument displays the next prompt, to which you should respond after the saline change is completed:...
  • Page 182 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument Open the front access door (❶) (see left figure below) to expose the dispenser station, then lift the saline access door (❷) to expose the saline bag. ❷ ❷ ❸...
  • Page 183 Maintaining the VITEK 2 Instrument Maintaining the VITEK 2 Disposables The entire assembly is now disconnected. Since they are Single Use Only items, you must discard the saline tube, its tubing, and the saline bag. NOTE: Since the saline dispenser tube does not come in con- tact with any specimen, it is not considered a biohaz- ard.
  • Page 184 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument Remove the covers from the large port of the saline bag and from the bag spike of the tubing assembly. 10. Insert the bag spike (❻) from the tubing assembly into the port (❼) of the saline bag.
  • Page 185 Maintaining the VITEK 2 Instrument Maintaining the VITEK 2 Disposables 14. Press the round plate (11) to allow saline to flow into the saline dispenser tube. Do not allow the saline level to fill above the top inlet port. (12) 15.
  • Page 186 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument 17. Press the Done option button. Change Saline Proceed with Saline change Done Cancel The following message appears: Change Saline Performing Dispenser self test The self test checks for proper installation of the saline dis- penser tube.
  • Page 187 Maintaining the VITEK 2 Instrument Maintaining the VITEK 2 Disposables 18. Perform the Dispenser/Pipettor diagnostic test, described on page 8-36, to ensure that the dispenser station is functioning properly. The VITEK 2 User’s Manual 7-13...

  • Page 188: How To Install An Accessory Kit, Part B: Pipette Tips

    Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument How to Install an Accessory Kit, Part B: Pipette Tips NOTE: If the card transport system is processing cassettes through the card transport station, the following mes- sage appears: Card transport system is busy.
  • Page 189 The following message appears: Change Pipette Tips Proceed with adding pipette tips Done Cancel Open the front access door (❶) of VITEK 2 to expose the Pipettor station. ❶ Open the Pipette Tip access door. The VITEK 2 User’s Manual 7-15...
  • Page 190 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument Rotate the pipette tip container (❷) 90° in a coun- terclockwise direction. ❷ ❸ Remove the pipette container from its base using a pulling motion. Discard any pipette tips that remain in the con- tainer.
  • Page 191 Maintaining the VITEK 2 Disposables NOTE: Discarding the remaining tips is required in order for VITEK 2 to maintain an accurate count of them. This action also minimizes the risk of contamination. Open a pipette tip replacement pack and hold it in one hand (see figure on following page).
  • Page 192 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument CAUTION: The exposed ends (❺) of the pipette tips are the ones that come in contact with your specimens. Appropriate precau- tions to prevent contamination during this procedure are advised.
  • Page 193 Maintaining the VITEK 2 Instrument Maintaining the VITEK 2 Disposables 12. Rotate the container 90° in a clockwise direction, making sure that it is fully seated in the down posi- tion. 13. Close both access doors. 14. Press the Done option button.
  • Page 194 Maintaining the VITEK 2 Disposables Maintaining the VITEK 2 Instrument The pipettor self test only checks the electric motors in the pipettor station. If the motors pass the test, no further message is displayed, and you are done with the installation. If a problem is found, the message “Pipette Tip installation error”...

  • Page 195: Cleaning The Carousel

    Qualified labo- ratory personnel should take the usual precautions necessary for infectious agents. NOTE: This procedure must be performed when the VITEK 2 instrument is completely idle (no cards are processing anywhere in the instrument).

  • Page 196: Vitek 2 Xl Carousel Cleaning Screen

    How to Remove the Carousel for Cleaning Maintaining the VITEK 2 Instrument If you have a VITEK 2 XL, the screen below displays allowing you to select a Reader. Carousel Cleaning Reader: A Reader: B Figure 7-2: VITEK 2 XL Carousel Cleaning Screen...
  • Page 197 Maintaining the VITEK 2 Instrument How to Remove the Carousel for Cleaning Press Continue. The following screen displays: Carousel Cleaning Remove the incubator access cover now Open the Waste Collection door for the carousel being cleaned. Open the top access door for the carousel being cleaned.
  • Page 198 How to Remove the Carousel for Cleaning Maintaining the VITEK 2 Instrument Remove the incu- bator access ❷ cover (❶) from the ❶ carousel by hold- ing the retaining pin (❷) on the top of the cover to the right while pulling...

  • Page 199: Removing A Carousel Section

    Maintaining the VITEK 2 Instrument How to Remove the Carousel for Cleaning When the carousel is in position, the following screen displays: Carousel Cleaning Remove Section Now Current number of Sections: 4 of 4 Press here after completion The carousel is removed in four sections. Grasp the first carousel section and pull it slightly forward (toward the front of the instrument).
  • Page 200 How to Remove the Carousel for Cleaning Maintaining the VITEK 2 Instrument Press the Press here after completion option but- ton to indicate removal of the carousel section. The following screen displays: Carousel Cleaning Current number of Sections: 3 of 4...
  • Page 201 Maintaining the VITEK 2 Instrument How to Remove the Carousel for Cleaning Press the Press here after completion option but- ton. The following screen displays: Carousel Cleaning Replace the incubator access cover now 10. Hold the retaining pin on the top of the cover to the right and carefully replace the incubator access cover over the carousel.

  • Page 202: How To Clean The Carousel

    How to Clean the Carousel The four sections of the carousel should be thoroughly cleaned and dried before they are replaced in the VITEK 2 instrument. The carousel material is designed to withstand any of the fol- lowing cleaning methods: •...

  • Page 203: How To Replace The Carousel After Cleaning

    Maintaining the VITEK 2 Instrument How to Replace the Carousel After Cleaning How to Replace the Carousel After Cleaning After the four carousel sections have been removed, the Clean- ing menu displays. Access the Carousel Cleaning function using the path: Cleaning ⇒...
  • Page 204 How to Replace the Carousel After Cleaning Maintaining the VITEK 2 Instrument Press Continue to continue with the carousel replacement. The following screen displays: Carousel Cleaning Remove the incubator access cover now Remove the incubator access cover. When the access cover has been removed, the following...
  • Page 205 Maintaining the VITEK 2 Instrument How to Replace the Carousel After Cleaning When the carousel is in position, the following screen dis- plays: Carousel Cleaning Replace Section Now Current number of Sections: 0 of 4 Press here after completion Take any one of the carousel sections and orient it as shown in the following figure.
  • Page 206 How to Replace the Carousel After Cleaning Maintaining the VITEK 2 Instrument Slide the section down along the base plate while maintaining the tilted angle. When the section has been pushed down all the way, release the top portion, allowing it to rest against the base plate.
  • Page 207 Maintaining the VITEK 2 Instrument How to Replace the Carousel After Cleaning Hold the retaining pin on the top of the cover to the right and carefully replace the incubator access cover over the carousel. Release the retaining pin when the cover is in place.

  • Page 208: Cleaning The Cassettes

    Cleaning the Cassettes Maintaining the VITEK 2 Instrument Cleaning the Cassettes Cassettes should be cleaned monthly, or as conditions require. How to Clean the Cassettes DANGER: The cassettes should be considered as potentially contami- nated and should be handled appropriately. Qualified labo- ratory personnel should take the usual precautions necessary for infectious agents.
  • Page 209 Maintaining the VITEK 2 Instrument Cleaning the Cassettes The cassette material is designed to withstand any of the fol- lowing cleaning methods. • Automatic dishwasher with standard laboratory detergent CAUTION: Dishwasher temperatures during the washing and drying cycles must not exceed 85° C (185° F). Exceeding this tem- perature will cause damage to the cassettes.

  • Page 210: Removing The Button Memory Module

    Cleaning the Cassettes Maintaining the VITEK 2 Instrument Turn an empty cassette over so that its underside is facing you, as shown in the figure below. Apply a slight inward pressure to the plastic tab (❶) that extends from the memory module (❷).
  • Page 211 Maintaining the VITEK 2 Instrument Cleaning the Cassettes NOTE: When cleaning multiple cassettes, it is not necessary to match a button memory module to a particular cas- sette. The units are completely interchangeable. The cassettes should be thoroughly cleaned and dried before they are used again.

  • Page 212: Replacing The Button Memory Module

    Cleaning the Cassettes Maintaining the VITEK 2 Instrument EPLACING THE UTTON EMORY ODULE Turn the cassette over and hold it in your right hand. Hold the memory module by the extended tab (❶) in your right hand so that the two metal contacts (❷) will line up with the two holes in the side of the cas- sette (❸).

  • Page 213: Cleaning The Boats

    Maintaining the VITEK 2 Instrument Cleaning the Boats Place the other end of the module against the hold- ing bracket (❹) and push the module towards the side of the cassette. The module should snap into place. Cleaning the Boats NOTE: The procedures in this section should be performed monthly, or as conditions require.
  • Page 214 Cleaning the Boats Maintaining the VITEK 2 Instrument Access the Boat Cleaning function using the path: Main Menu Utilities Maintenance Cleaning ⇒ ⇒ ⇒ Boat Cleaning ⇒ The following screen appears: Boat Cleaning Current number of Boats: 4 of 4.
  • Page 215 Maintaining the VITEK 2 Instrument Cleaning the Boats When the boat is ready for removal, the following screen appears: Boat Cleaning Boat is at the Boat Dock. Please remove it. Press here after completion Cancel Open the front access door (see figure at right).

  • Page 216: How To Clean The Boats

    Maintaining the VITEK 2 Instrument How to Clean the Boats The four boats should be thoroughly cleaned and dried before they are replaced in the VITEK 2 instrument. The boat material is designed to withstand any of the following cleaning methods. •...
  • Page 217 Maintaining the VITEK 2 Instrument Cleaning the Boats Access the Boat Cleaning function using the path: Main Menu Utilities Maintenance Cleaning ⇒ ⇒ ⇒ ⇒ Boat Cleaning The following prompts appear: Boat Cleaning Current number of Boats: 0 of 4.
  • Page 218 Cleaning the Boats Maintaining the VITEK 2 Instrument If the system is idle, the instrument prepares for the boat replacement, and then displays this prompt: Boat Cleaning Replace Boat Press here after completion Cancel Open the front access door and replace the boat below the Pipettor station.
  • Page 219 Maintaining the VITEK 2 Instrument Cleaning the Boats Press the option button shown below after replac- ing the boat. Press here after completion The first screen reappears with the boat count at “1 of 4.” Repeat this procedure, starting at step 2, until all four boats have been replaced.

  • Page 220: Cleaning The Inside Of The Vitek 2 Instrument

    Cleaning the Inside of the VITEK 2 Instrument Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument DANGER: All organism suspensions should be considered as poten- tially infectious. Qualified laboratory personnel should use acceptable procedures for biohazardous material.
  • Page 221 Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument If any cards are processing the instrument displays this screen: Cards are currently processing. Do you wish to continue? CAUTION: If you select Yes to continue, the cards being processed will be TERMINATED.
  • Page 222 Cleaning the Inside of the VITEK 2 Instrument Maintaining the VITEK 2 Instrument Press the Yes option button to shut down the instru- ment. The instrument briefly displays the message Shutting Down Instrument while it moves some internal components to their shut- down positions.

  • Page 223: Cleaning The Test Card Collection Tray

    Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument Cleaning the Test Card Collection Tray Open the Waste Collection door and remove the test card collection tray. Thoroughly clean and dry the tray before replacing it in the VITEK 2 instrument.

  • Page 224: Cleaning The Optics (Normal Maintenance)

    Cleaning the Inside of the VITEK 2 Instrument Maintaining the VITEK 2 Instrument Cleaning the Optics (Normal Maintenance) NOTE: Perform procedure Shutting Down the VITEK 2 Instrument on page 7-46 before proceeding with this procedure. DANGER: The instrument contains an ultraviolet light source. Make sure that the fluorescence optics unit is closed before resum- ing instrument operation.
  • Page 225 Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument Both optics consist of units that are hinged on the bottom and held in place by spring-loaded levers. The transmittance optics consists of two of these units while the fluorescence optics have only one.
  • Page 226 Cleaning the Inside of the VITEK 2 Instrument Maintaining the VITEK 2 Instrument If foreign material remains on either surface, repeat this step using an alcohol wipe. Squeeze out excess alcohol before using, and dry the surface with lens paper. Make sure that you do not leave streaks on the glass.

  • Page 227: Cleaning The Base Pan, Vacuum Seal And Vacuum Chamber

    Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument Cleaning the Base Pan, Vacuum Seal and Vacuum Chamber NOTE: Perform the instrument shutdown procedure on page 7-46 before proceeding with this procedure. NOTE: If the boats have not already been removed, perform the procedure to remove them.
  • Page 228 Cleaning the Inside of the VITEK 2 Instrument Maintaining the VITEK 2 Instrument DANGER: The base pan should not be cleaned unless the drip pan is properly installed. See 7-56 for instructions on cleaning the drip pan. The base pan, vacuum seal, and vacuum chamber are designed to withstand any of the following cleaning methods.
  • Page 229 Maintaining the VITEK 2 Instrument Cleaning the Inside of the VITEK 2 Instrument Using a solution of disinfectant and warm water, wipe off any dust or dirt from the surface of the base pan. Particular attention should be paid to the area beneath the Dispenser/Pipettor station, where drips or spills may have occurred.

  • Page 230: Cleaning The Drip Pan

    NOTE: This procedure should be performed monthly, or as conditions require. VITEK 2 has a small container, called the drip pan, located underneath the Dispenser/Pipettor station. The pan is designed to catch any spills that occur at this station.

  • Page 231: Removing The Drip Pan

    Maintaining the VITEK 2 Instrument Cleaning the Drip Pan Removing the Drip Pan Lower the bottom access door located in the bot- tom, front portion of the instrument. The plate is held magnetically and should swing down on its hinges when you pull on it at both ends.

  • Page 232: How To Clean The Drip Pan

    Maintaining the VITEK 2 Instrument How to Clean the Drip Pan The drip pan should be thoroughly cleaned and dried before it is replaced in the VITEK 2 instrument. The pan material is designed to withstand any of the following cleaning methods: •...

  • Page 233: Turning The Instrument On

    Maintaining the VITEK 2 Instrument Cleaning the Drip Pan Turning the Instrument On Move the AC power switch to the ON position. The system goes through the initialization process. When the Status Screen appears it will display a status of Clean- ing, indicating that all four boats have been removed.

  • Page 234: Cleaning The Smart Carrier Station

    Cleaning the Smart Carrier Station Maintaining the VITEK 2 Instrument Cleaning the Smart Carrier Station DANGER: All organism suspensions should be considered as poten- tially infectious. Qualified laboratory personnel should use acceptable procedures for biohazardous material. NOTE: This procedure should be performed monthly, or as conditions require.
  • Page 235 Maintaining the VITEK 2 Instrument Cleaning the Smart Carrier Station Remove the SCS AC power cord from its outlet. Clean all surfaces of the SCS base unit, keypad, dis- play, and bar code scanner, except the glass lens of the scanner.
  • Page 236 Cleaning the Smart Carrier Station Maintaining the VITEK 2 Instrument The VITEK 2 and VITEK 2 XL Maintenance Logs on the fol- lowing pages lists recommended maintenance procedures and their frequency. Make one copy for your use and keep the origi- nal in this manual.

  • Page 241: Introduction

    Error Messages and Recovery Procedures This section contains a listing of all the possible error messages that could occur on the VITEK 2 instrument. This is the first place you should go to if an error occurs and you need to find out what to do.
  • Page 242 – Inoculum Errors 8-24 – Card Capacity Errors 8-26 – Disposables Errors 8-29 • Working Without the VITEK 2 Bar Code Reader 8-30 – How to Enter Bar Codes Manually 8-31 • Instrument Diagnostics 8-33 – Instrument Diagnostics Menu 8-33 –...

  • Page 243: The Error Handling System

    If a sensor detects a problem, the VITEK 2 instrument is programmed to alert you to the situa- tion, and to provide you with the information you need to resolve the problem. We refer to this process as the Error Han- dling System.
  • Page 244 The Error Handling System Troubleshooting the VITEK 2 Instrument The first element of the Error Handling System is the VITEK 2 instrument. First it must detect the error. In many cases, the instrument will attempt to correct the problem by retrying an operation.

  • Page 245: The Error Alarms

    The Error Handling System The Error Alarms VITEK 2 has two alarms to alert you to the presence of an error condition. The dual alarms are provided in case the VITEK 2 instrument and the workstation are in different locations. When they are in close proximity, the audible alarm on the instrument can be disabled as a configuration option.

  • Page 246: Types Of Errors

    The Error Handling System Troubleshooting the VITEK 2 Instrument Types of Errors There are three basic types of errors: • those that cause the Reader or Card Transport system to halt • those that do not cause the Reader or Card Transport system to halt •...

  • Page 247: The Status Screen In An Error Condition

    Troubleshooting the VITEK 2 Instrument The Error Handling System The Status Screen in an Error Condition Depending on the type of error, you will see one or more types of screens. • Instrument Halted Screen Indicates an error has caused the Reader or Card Transport system to halt.

  • Page 248: Instrument Halted Status Screen

    The Error Handling System Troubleshooting the VITEK 2 Instrument NSTRUMENT ALTED SCREEN Instrument Halted ❶ Reader: (Halted/Processing) ❷ Card Transport: (Halted/Processing) ❸ Minutes: ❹ Messages Pending: ❻ ❺ 12:00 Figure 8-2: Instrument Halted Status Screen ❶ Reader. This field indicates whether the Reader station is still processing, or has been halted due to the error.

  • Page 249: The Vitek 2 Status Screen

    (❶) has changed to “Messages,” indicating that there are error messages in the error/message queue. 12:00 ❶ Status: Messages MAX Available Slots: 60 Cassette Name: NSB Dilution Mode: Automatic Tips Saline Figure 8-3: The VITEK 2 Status Screen The VITEK 2 User’s Manual...
  • Page 250 The Error Handling System Troubleshooting the VITEK 2 Instrument Press the Error/Message key (?) to view the error/message queue. If there is more than one message or error, press the Down Arrow to review and address each message. Message Queue ❶...
  • Page 251 MAX Available Slots: 60 Cassette Name: NSB Dilution Mode: Automatic Tips Saline Review the error/message queue for any error messages the VITEK 2 instrument has generated. Resolve the problem as indicated in Table 8-2. The VITEK 2 User’s Manual 8-11...

  • Page 252: Reviewing An Instrument Halted Screen

    The Error Handling System Troubleshooting the VITEK 2 Instrument Reviewing an Instrument Halted Screen Press the Error/Message key to view the error/mes- sage queue. NOTE: If there is more than one message, use the DOWN ARROW to review and address each message.

  • Page 253: Reviewing A Status Screen With Messages

    Troubleshooting the VITEK 2 Instrument The Error Handling System Reviewing a Status Screen with Messages Press the Error/Message key to view the error/mes- sage queue. NOTE: If there is more than one message, use the DOWN ARROW to review and address each message.

  • Page 254: Reviewing A Status Screen With Errors

    The Error Handling System Troubleshooting the VITEK 2 Instrument Reviewing a Status Screen with Errors When the status of ‘Messages’ has changed to ‘Errors’ this indi- cates one of two conditions: • The message was reviewed but not resolved Follow Steps 1 through 3 in the procedure for Reviewing a Status Screen with Messages.

  • Page 255: Cassette Load Processing Errors

    Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors Cassette Load Processing Errors Cassette load processing errors are a special set of errors that occur immediately following the loading of a cassette. You should be familiar with this type of error because: •...

  • Page 256: Why Do They Occur

    For example, the button memory told VITEK 2 that a gram negative susceptibility card is in slot 4 of the cassette. The bar code reader, however, found that slot to be empty.

  • Page 257: Work Flow Considerations

    Cassette Load Processing Errors Work Flow Considerations For any type of load error, the VITEK 2 instrument backs the cassette out to the Load station so that you can resolve the error. If the cassette loading door is not opened within 10 minutes, the instrument assumes that no corrective action will be taken.

  • Page 258: Bar Code Error Message

    Cassette Load Processing Errors Troubleshooting the VITEK 2 Instrument OW TO ESOLVE A RROR Press the Question Mark key to display the Error queue. For a bar code error, you see the following message: Error Queue Processing Error Bar Code Read Failure...

  • Page 259: Screen For Resolving Bar Code Errors

    Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors Remove the card from the slot indicated in the mes- sage. Press the PREVIOUS SCREEN key twice to exit the error queue to the Main Menu. Access the Resolve Bar Code Errors option on the Main Menu.
  • Page 260 NOTE: The bar code you enter may be longer than the space provided on the screen. When this occurs, the first portion of the bar code scrolls off the display and the symbol “>” appears. VITEK 2 checks your entry for: • A valid bar code number. •...

  • Page 261: Scs And Bar Code Reader Conflict

    Cassette Load Processing Errors SCS and Bar Code Reader Conflict This type of error only occurs when VITEK 2 is operating in Smart Carrier mode. That is because the error is based on a comparison of the information read from the bar codes with that read from the cassette memory.

  • Page 262: Card Type Error Message

    Cassette Load Processing Errors Troubleshooting the VITEK 2 Instrument OW TO ESOLVE A EADER FLICT Press the Error/Message key to display the error/ message queue. For a card type error you see the following screen: Message Queue Processing Error SCS and Bar Code Reader...
  • Page 263 Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors Press the F3 key to display the Summary screen. Check the positions and the types of test cards against the data displayed on the screen. Move the test cards and their dilution tubes, as nec- essary, to match the data on the screen.

  • Page 264: Inoculum Errors

    Cassette Load Processing Errors Troubleshooting the VITEK 2 Instrument Inoculum Errors When VITEK 2 is operating in the Automatic dilution mode, every susceptibility test card must be provided with an inocu- lum source. The inoculum must be placed in the slot that immediately precedes the susceptibility card.

  • Page 265: Inoculum Source Error Message

    Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors OW TO ESOLVE AN NOCULUM RROR Press the Error/Message key to display the mes- sage queue. For an inoculum source error you see the following screen Message Queue Processing Error Inoculum source not...

  • Page 266: Card Capacity Errors

    Cassette Load Processing Errors Troubleshooting the VITEK 2 Instrument Place the inoculum source next to the appropriate slot. Place the cassette back into the Cassette Load sta- tion and close the door. This restarts the processing cycle for the cassette.

  • Page 267: Card Capacity Warning Message

    Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors OW TO ESOLVE A APACITY RROR Press the Error/Message key to display the error/ message queue. For a card capacity error you see the following screen: Message Queue Processing Warning Insufficient slots...
  • Page 268 Cassette Load Processing Errors Troubleshooting the VITEK 2 Instrument Consult the card directory at the workstation. Count the number of cards whose tests have finalized and that can be ejected. Eject those cards using the workstation software. If there are still too many test cards in the current cassette, and remove the needed number of cards.

  • Page 269: Disposables Errors

    Troubleshooting the VITEK 2 Instrument Cassette Load Processing Errors Disposables Errors When a cassette begins processing, VITEK 2 calculates the quantity of disposables that will be needed. If the calculated value exceeds the current level of a disposable: • The cassette is backed out to the Cassette Load station.

  • Page 270: Working Without The Vitek 2 Bar Code Reader

    A good reading of the test card bar code is required by the sys- tem to know the card type and how to process it. The primary method of entering these bar codes is the Bar Code Reader sta- tion in the VITEK 2 instrument. There are also two backup methods: •...

  • Page 271: How To Enter Bar Codes Manually

    Working Without the VITEK 2 Bar Code Reader How to Enter Bar Codes Manually Failure of the Bar Code Reader station on the VITEK 2 instru- ment would result in a bar code read error for every card pro- cessed. This can be avoided by disabling the station, and then entering bar codes via the Smart Carrier Station, or manually on the VITEK 2 keypad.
  • Page 272 Working Without the VITEK 2 Bar Code Reader Troubleshooting the VITEK 2 Instrument NTER ODES ANUALLY Access the Resolve Bar Code Errors option on the Main Menu. The following screen appears: Bar Code: Slot __ Clear Using the numeric keypad, enter the bar code from the bar code label.

  • Page 273: Instrument Diagnostics

    Cassette. Instrument Diagnostics Some of the error recovery procedures in this chapter ask you to perform a diagnostic test on one of the stations in the VITEK 2 instrument. This section tells you how to access and use those tests.

  • Page 274: Diagnostic Tests

    Instrument Diagnostics Troubleshooting the VITEK 2 Instrument Diagnostic Tests Following are the tests found on the Diagnostics menu, and their function: Diagnostic Test Function Possible Results Temperature Reads the temperature in Displays the carousel the reader carousel. temperature in degrees Celsius.
  • Page 275 ⇒ Utilities ⇒ Diagnostics ⇒ Temperature If you have a VITEK 2 XL, the screen below displays: Temperature Incubator A: 35.5° C Incubator B: 35.5° C Inside: 28.0° C If you have a VITEK 2, the screen below displays: Temperature Incubator: 35.5°...
  • Page 276 Instrument Diagnostics Troubleshooting the VITEK 2 Instrument ISPENSER IPETTOR IAGNOSTIC NOTE: The transport system must be idle to perform this test. This test is designed to be performed: • after installing an Accessory Kit to confirm correct opera- tion of the dispenser/pipettor station.
  • Page 277 Troubleshooting the VITEK 2 Instrument Instrument Diagnostics The following message displays: About to perform dispenser pipettor test. Do you wish to continue? Press the Yes option button. A message on the display will instruct you to load the cas- sette that you prepared in step 1.
  • Page 278 Instrument Diagnostics Troubleshooting the VITEK 2 Instrument • Look for saline remaining in the saline dispenser tube when it returns to the upright position. • Look for a saline stream and/or drops released from the saline dispenser tube when it is filling with saline •...
  • Page 279 Troubleshooting the VITEK 2 Instrument Instrument Diagnostics ISPENSER IPETTOR OLUMETRIC This test is not required by bioMérieux as part of the recom- mended routine maintenance procedures. This test has been provided for those laboratories who are required to verify the accuracy of the volumes dispensed by the Pipettor/Dispenser by regulatory/licensing agencies.
  • Page 280 Instrument Diagnostics Troubleshooting the VITEK 2 Instrument Use this table to record the weights of your test tubes. Make one copy for your use and keep the original in this manual. Date Performed Pipettor Low Volume Results Tested Test tube 1 - Before test (B)
  • Page 281 Troubleshooting the VITEK 2 Instrument Instrument Diagnostics Access the Dispenser/Pipettor diagnostic test using the path: Main Menu Utilities Diagnostics ⇒ ⇒ ⇒ Dispenser/Pipettor The following message displays: About to perform dispenser pipettor test. Do you wish to continue? Press the Yes option button.
  • Page 282 Instrument Diagnostics Troubleshooting the VITEK 2 Instrument Determine if the Dispenser/Pipettor is performing to specification by performing the following calculations using Table 8-1: • In the Pipettor Low Volume section, subtract the weight of tube 1(A) from the weight of 1(B). The acceptable value must be between .095 gm to .105 gm.

  • Page 283: Cleaning And Verifying The Optics With Cards Processing

    Troubleshooting the VITEK 2 InstrumentCleaning and Verifying the Optics with Cards Processing Cleaning and Verifying the Optics with Cards Processing This procedure allows you to clean and verify the optics while test cards are still processing. Use the Optics Cleaning proce- dure in Chapter 7 if the instrument is shut down.
  • Page 284 Cleaning and Verifying the Optics with Cards ProcessingTroubleshooting the VITEK 2 Instrument If cards are processing, the following screen appears: Optics Cleaning Cards are currently processing. Do you wish to continue? Press the Yes option button to continue. The screen briefly displays the message...
  • Page 285 Troubleshooting the VITEK 2 InstrumentCleaning and Verifying the Optics with Cards Processing You have five (5) minutes from the time that you press the Yes option button to complete the cleaning procedure. Test card processing automatically resumes after that time.
  • Page 286 Cleaning and Verifying the Optics with Cards ProcessingTroubleshooting the VITEK 2 Instrument Lift the top access door of the instrument. This exposes both the fluores- cence (❶) and the transmittance ❶ ❷ (❷) optics, shown in the figure to the right.
  • Page 287 Troubleshooting the VITEK 2 InstrumentCleaning and Verifying the Optics with Cards Processing Inspect the glass on both surfaces for cracks or scratches. NOTE: Any apparent crack or scratch should be reported to bioMérieux. Using a quality, lint free lens paper that has been slightly moistened with a commercial glass cleaner, clean the glass surfaces.

  • Page 288: Optical Diagnostic Test

    Cleaning and Verifying the Optics with Cards ProcessingTroubleshooting the VITEK 2 Instrument 12. Press the Cleaning completed option button that was displayed in the screen in step 2. NOTES: If step 12 is not completed within the five minutes allowed, test card processing resumes automatically.
  • Page 289 Troubleshooting the VITEK 2 InstrumentCleaning and Verifying the Optics with Cards Processing The following screen appears for either the VITEK 2 or VITEK 2 XL Optical Test About to perform optical test. Do you wish to continue? Press Yes to continue.

  • Page 290: Boat Transport Positions

    Boat Transport Positions Troubleshooting the VITEK 2 Instrument Boat Transport Positions The diagram below shows the six boat transport positions. It is important to know the boat positions inside the instrument to aid in the troubleshooting of jams involving the card transport system.
  • Page 291 Troubleshooting the VITEK 2 Instrument Boat Transport Positions ❶ Cassette Load/Unload station. NOTE: Boats must not be removed or replaced at this station. ❷ ❸ This is the area you see when you open the front access door. ❷ Button Memory Station ❸...

  • Page 292: Power Failures

    Troubleshooting the VITEK 2 Instrument Power Failures What happens in the VITEK 2 instrument if there is a power failure? The instrument is supported by a device called an Unin- terruptible Power Supply (UPS). If power is lost, the UPS uses a battery to continue to supply power to the instrument.
  • Page 293 If power is restored within the 20 minute period, normal opera- tion is resumed. If the power failure continues after the 20 minute period has expired, VITEK 2 shuts down the card trans- port system but continues to process cards in the carousel. Car- ousel processing continues as long as the battery in the UPS supplies power.

  • Page 294: Displaying Version Information

    Troubleshooting the VITEK 2 Instrument Displaying Version Information Allows you to view the current instrument firmware for the VITEK 2. You may be requested to supply this information to bioMérieux if you need assistance with any of the recovery pro- cedures in Table 8-2.

  • Page 295: How To Use Error Message And Recovery Table

    Troubleshooting the VITEK 2 Instrument How to Use Error Message and Recovery Table How to Use Error Message and Recovery Table Table 8-2 in this section contains all of the messages that can be displayed in the error/message queue. The Error Message and Recovery table is designed to provide you, where possible, with the recovery procedure that is most likely to restore the instrument to normal operation.

  • Page 296: How To Restart The Instrument

    How to Use Error Message and Recovery Table Troubleshooting the VITEK 2 Instrument How to restart the instrument Several of the recovery procedures in the table instruct you to restart the instrument. To do this: Perform the instrument shutdown procedure on page 7-46.
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  • Page 317: Appendix: Hardware Specifications

    Approximately 145 kg (240 lb) kg (320 lb) CAUTION: The VITEK 2 instrument is heavy. Use at least two people to move it. Lift handles are provided at each end of the instru- ment. The VITEK 2 User’s Manual Appendix-1...
  • Page 318 Appendix: Hardware Specifications Environment NOTE: The specifications below apply to both the VITEK 2 and the VITEK 2 XL. Indoor usage Do not install in direct sunlight Benchtop should be level within 2° Minimum of 5 cm (2 in) clearance on all sides for adequate ven- tilation;...
  • Page 319 200 watts nominal, 569 watts peak watts peak Heat 512 BTU/HR (nominal) 682 BTU/HR (nominal) Power cord Detachable 3-wire with Detachable 3-wire with ground, per IEC 227 or ground, per IEC 227 or IEC 245 IEC 245 The VITEK 2 User’s Manual Appendix-3...
  • Page 320 Appendix: Hardware Specifications Optical Characteristics NOTE: The specifications below apply to both the VITEK 2 and the VITEK 2 XL. RANSMITTANCE PTICS Emission wavelengths: 660 nm, 590 nm, 568 nm, 430 nm LED Percent transmission range: 30% to 100% ± 10%...
  • Page 321 Appendix: Hardware Specifications General Characteristics NOTE: The specifications below apply to both the VITEK 2 and the VITEK 2 XL. ASSETTE Capacity, cassettes: 4 cassettes maximum Capacity, cards: 15 cards per cassette ISPENSER Dispensing volume: 2.33 - 2.63 ml Capacity: 1000 ml, user replaceable IPETTOR Dispensing volume: 60 µl to 300 µl ±...
  • Page 322 Appendix: Hardware Specifications Smart Carrier Station Dimensions NOTE: The specifications for the Smart Carrier Station apply to both the VITEK 2 and the VITEK 2 XL. Width: 27.15 cm (10.7 inches) Height: 28.26 cm (11.1 inches) Depth: 17.78 cm ( 7 inches) Mass Approximately 2.6 kg (5.85 lbs.)
  • Page 323 Fused input current: 2 amp - T (GDC-2) Nominal input current: .2 amps @ 120 VAC .1 amps @ 240 VAC Power: 10 watts nominal Heat: 34 BTU/HR (nominal) Power Cord: Detachable 3 wire/g, per IEC 227 or IEC 245 The VITEK 2 User’s Manual Appendix-7...

  • Page 325: Glossary

    (and unload) door, and waste collection access door. Access doors VITEK 2 XL VITEK 2 XL has nine doors to enable the operator to access the internal systems and stations to replenish supplies and perform maintenance and cleaning procedures: top access door section...
  • Page 326 Antimicrobial Susceptibility Testing (AST) Testing the susceptibility of certain bacteria to known antibiotic concentrations. VITEK 2 helps the diagnostician determine the best medical treatment for the patient’s specific strain of micro- organism tested.
  • Page 327 Bar code An alphanumeric interface identifier label affixed to each VITEK 2 test card. The bar code contains the card’s test type, lot number, and expiration date, which is entered either by the SCS subsystem by bar code wanding, or when the test card’s bar code label is read by the bar code reader inside of the VITEK 2 instrument.
  • Page 328 Smart Carrier Station to the VITEK 2 workstation computer. Card transport system Moves the test cards among the stations within the VITEK 2 instrument. It consists of the cassette tray, boat, cassette loading and unloading station, electromechanical arms, card ejectors, and the waste collector station.
  • Page 329 (60 uL to 300uL - see aliquot) to adjacent susceptibility tubes. The system includes a container for pipette tips, a displacement pump, and various tip mecha- nisms. The VITEK 2 User’s Manual Glossary-5...
  • Page 330 Glossary Disposables VITEK 2 disposables are pipette tips, sterile saline, test cards, and inoculum tubes. Disposable supplies are biohazardous after use and must be dis- carded according to local laws regarding biohazardous material. They should not be recycled or disposed of with normal trash, etc.
  • Page 331 35.5° C. Instrument Bar code reader This station reads the bar code label on each VITEK 2 test card within the first minute after the test cards are read, and transfers all of the test card bar code data to the VITEK 2 Workstation computer.
  • Page 332 Glossary Pre-diluted Mode One of two possible settings for the Dilution Mode configura- tion on the VITEK 2 instrument. In Pre-diluted mode, the user prepares AST suspensions prior to loading test cards on the instrument. See Automatic Dilution Mode. Saline Dispenser Tube A “fixed volume”...
  • Page 333 Test card A 3-1/2 by 2-1/4 inch (76.2 by 49.45 mm) cassette card with 64 growth wells for biological testing in the VITEK 2 system. Each well contains an air-dried suspension of a biochemical or drug used to identify and suggest treatment for microbial infec- tions in patients.
  • Page 334 Also see fluorescence optics. User interface (UIF) The portion of the VITEK 2 system in which the user and the system’s software interact. The system has three user interfaces: •...
  • Page 335 The workstation computer is the primary processor used in cal- culating test results. The workstation can also be used as a means to interface the VITEK 2 system with a central labora- tory or hospital computer system. The workstation computer provides the user with the graphical interface used with the mouse pointing device and workstation monitor, and a text interface for the keyboard.

  • Page 337: Index

    6-55 Bar code scanner, SCS 2-5, 6-13, 6-24 Cassette address 6-35, 6-49 Bar codes, entering manually 8-31 Cassette erase 3-10 Base pan, cleaning 7-53 Cassette icon 6-8 Base unit, SCS 2-4 Cassette mode 5-10 The VITEK 2 User’s Manual Index-1...
  • Page 338 8-24 Contrast, screen 5-17 instrument capacity 8-26 setting 5-25 messages 8-55 recovering from 8-55 types 8-6 Data entry, cassette 6-28 using the status screen 8-7 without a SCS 6-48 what to do 8-3–8-14, 8-55 Index-2 The VITEK 2 User’s Manual...
  • Page 339 Instrument Halted Screen 8-8 Name Instrument icon 6-8 cassette 5-5 Instrument name 5-7 cassette, defining 5-20 defining 5-20 instrument 5-7 Isolate number field 6-16 instrument, defining 5-20 station, setting 3-5 Note, definition of 1-5 The VITEK 2 User’s Manual Index-3...
  • Page 340 7-39 starting 2-7 button memory from a cassette 7-36 using 6-13 carousel 7-21 work flow description 6-26 drip pan 7-57 working without 6-36 ejected card 6-57 Sealer station 4-23 waste collection tray 4-28 Index-4 The VITEK 2 User’s Manual...
  • Page 341 Version information 8-54 Shutting down the instrument 7-46 Visual alarm 5-15 Slot indicator, SCS 6-19 enabling 5-24 Smart Carrier Station, See SCS Vitek 2 Instrument, See Instrument. Starting the SCS 2-7 Volume Station name (SCS), setting 3-5 audible alarm 5-14 Station, instrument...

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