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Biotek ELx808 Manuals
Manuals and User Guides for Biotek ELx808. We have
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Biotek ELx808 manual available for free PDF download: Operator's Manual
Biotek ELx808 Operator's Manual (172 pages)
Absorbance Microplate Reader
Brand:
Biotek
| Category:
Laboratory Equipment
| Size: 6.15 MB
Table of Contents
All Rights Reserved
4
Restrictions and Liabilities
4
Trademarks
4
Table of Contents
5
Contact Information
10
Customer Service and Sales
10
European Coordination Center/Authorized European Representative
10
Service/Tac
10
Revision History
11
Document Conventions
14
Intended Use Statement
14
Quality Control
14
Repackaging and Shipping
15
Warnings
15
Warranty & Product Registration
15
Hazards
16
Precautions
17
CE Mark
18
Directive 2004/108/EC: Electromagnetic Compatibility
18
Directive 2006/95/EC Low Voltage (Safety)
18
Canadian Department of Communications Class a
19
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
19
Directive 98/79/EC: in Vitro Diagnostics (if Labeled for this Use)
19
Electromagnetic Interference and Susceptibility
19
Usa Fcc Class a
19
User Safety
20
Safety Symbols
21
Chapter 1: Introduction
23
Introducing the Elx808 Absorbance Microplate Reader
24
Reader Variations
24
Hardware Features
24
Elx808 Absorbance Microplate Reader
24
Software Features
25
Package Contents
25
Optional Accessories
25
Specifications
26
Microplates
26
Electrical
26
Physical
26
Environmental
27
Hardware
27
Reading Speeds
27
Standard Model: Elx808
27
Ultraviolet/Incubator Model - Elx808Iu
28
Incubator/Ultraviolet Model: Elx808Iu
28
Product Support & Service
29
Contacting the Technical Assistance Center
29
Returning Instruments for Service/Repair
29
Product Support and Service
29
Chapter 2: Installation
31
Product Registration
32
1: Unpack and Inspect the Instrument
32
2: Select the Operating Environment
33
3: Install the Filter Wheel
34
4: Check/Adjust the Power Input Voltage Setting
36
5: Connect Power
36
(Optional) Connect Printer
37
Printers
38
7: Turn on Reader and Run System Test
38
8: Check/Adjust the Reader's Filter Table
39
9: Configure Reader Settings
40
SETUP Options
40
OUTPUT Options
41
REPORT Type
41
READ Options
42
Read Speed
43
10: Install Software/Connect to a Computer (Optional)
43
Attach the Cable
43
Install Gen5 Software on the Host Computer
43
10: Install Software/Connect to Computer (Optional)
43
Establish Communication
44
Changing the Baud Rate on the Elx808
44
11: Verify Performance
45
Before Repackaging the Instrument
46
Chapter 3: Operation
47
Getting Started with Gen5
49
Introduction
50
The Keypad
51
The Startup Screen
52
The Main Menu Screen
52
Quick Read
53
Defining Assays
54
Selecting an Assay
54
Assay Name
54
Defining the Method, Map, Formula, and Curve
55
Method
56
Read Type
56
Delay in First Read
56
Incubation Temperature
57
Single or Dual Wavelength
57
MEAS Selection
58
Number of Kinetic Reads/Kinetic Duration Selection
58
Kinetic Interval
58
Kinetic Number of Reads
59
Kinetic Duration
59
Shake Mode Selection
59
Shake Time
60
Shake Speed
60
Kinetic Data Analysis Selection
60
Number of Kinetic Points Selection
61
Onset OD Selection
61
Linear Scanning
61
Map
62
Map Generation
63
Mapping Direction
64
Replication Direction
64
Examples of Mapping & Replication Directions
65
Start Mapping at Well Location
66
Selecting a Blank Map
66
Blank Map Definitions
67
Constant Blank Value Entry
68
Number of Blanks
68
Selecting a Blank Location
68
Number of Standards
69
Number of Standard Replicates
69
Average Standards
69
Standard Concentration
70
Reuse of Standard Curves
71
Number of Controls
72
Control Type
72
Number of Control Replicates
73
Location of Controls
73
Valid Well Locations
73
Number of Samples
74
Number of Sample Replicates
74
Sample Location
74
Formula
75
Validation Formula Examples
75
Formula Type
76
Validation Type Selection
77
Formula Entry
77
Number of Required Controls/Blanks
79
Cutoff Formulas
80
Greyzone Entry
81
Positive/Negative Calls for Cutoff
81
Examples
82
Transformation Formulas
83
Transformation Formula Definition
83
Transformation Scope Variable
84
Another Transformation Example
85
Curve
86
Curve Fit
86
Edit Standard Outliers
87
Axis Selection
88
Extrapolation of Unknowns
88
Panel Assays
89
Reading a Microplate
92
Select Assay
92
Run-Time Prompts
92
Enter Number of Samples
93
Enter Plate ID
93
Enter Sample ID
93
Prompts for Well Location
94
Beginning the Plate Read
94
Printing Reports and Assay Lists
95
Result
96
Editing Standard Outliers
96
Printing Results
97
Map
97
Assay
98
List
98
Recommendations for Optimum Performance
98
Chapter 4: Instrument Qualification
101
Overview
102
Recommended Qualification Schedule
102
Test Descriptions
103
System Test
103
Incubator Self Test
106
Checksum Test
106
Absorbance Plate Test
107
Empty Carrier Test
110
Liquid Tests
110
Perform the Tests
111
System Test
111
Checksum Test
111
Absorbance Plate Test
111
Define the Test Plate Parameters
111
Run the Plate Test
112
Empty Carrier Test
113
Liquid Tests
114
Prepare Test Solutions
114
Stock Solution Formulation
114
Solution a
114
Solution B
115
Perform Liquid Test 1
116
Calculations
116
Accuracy Specifications
117
Perform Liquid Test 2
117
Perform the Linearity Test (Test A)
118
Calculate Repeatability (Test B)
119
Channel-To-Channel Variation and Alignment (Test C)
119
Perform Liquid Test 3 ("UV" Models Only)
120
Perform the Test
121
Calculate Repeatability
121
Calculate Linearity
122
Chapter 5: Preventive Maintenance
123
Recommended Maintenance Schedule
124
Warnings and Precautions
124
Clean Exposed Surfaces
125
Inspect and Clean the Wavelength Filters
126
(Optional) Lubricate Robotic Components
127
Robotic Motor Adjustment Procedure
129
Replace the Lamp and Clean the Contacts
129
Decontamination
133
Purpose
133
Tools and Supplies
134
Decontamination Procedure
134
Chapter 6: Troubleshooting and Error Codes
137
Overview
138
Diagnostics
138
Terms Used in the Error Codes Tables
138
Error Codes (General)
139
Error Codes (Fatal)
143
Appendix A: Sample Reports
145
Samples with Calls on Matrix Report
146
Curve Fit Report
147
Samples with Calls on Column Report
148
Column Report Without Samples
149
Panel Report
150
Assay Detail Report
151
Assay List
152
Appendix B: Instructions for Programming a New Assay
153
Appendix C: Adjusting the Power Input Voltage Setting
163
Identifying the Reader's Power Input Module
164
Newer-Style Module
165
Adjust the Power Input Voltage Setting
165
Reconfigure or Replace Fuses
167
Older-Style Module
169
Adjust the Power Input Voltage Setting
169
Reconfigure or Replace the Fuses
170
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