For more information on emission filters, see the GE Healthcare publication
Fluorescence Imaging principles and methods.
A.4 Beamsplitters
The Typhoon instrument contains three standard beamsplitters and has a slot for one
additional custom beam splitter. You can use the beamsplitters with multilabel
fluorescent samples to create multichannel images. Table A-3 lists the three standard
beamsplitters.
Table A-3. Standard beamsplitters.
Beamsplitter
560-nm dichroic
580-nm dichroic
630-nm dichroic
Typhoon Operating Instructions 28-9623-56 AA
Reflects light of wavelengths shorter than 560 nm and
passes light longer than 560 nm
Reflects light of wavelengths shorter than 580 nm and
passes light longer than 580 nm.
Reflects light of wavelengths shorter than 630 nm and
passes light longer than 630 nm.
Accessories Appendix A
Beamsplitters A.4
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