Troubleshooting; Uv Curve Problems - GE AKTAprime plus Operating Instructions Manual

6 Troubleshooting

6 Troubleshooting
6.1

UV curve problems

Error symptom
Ghost peak
Noisy UV-signal, sig-
nal drift or instability
50
Possible cause
Dirt or residues in the
flow path from previ-
ous runs. Air in the
eluents.
Residue in the column
from previous runs
Incorrect mixer func-
tion
Dirty UV cell
Impure buffer
Air in the pump or in
the UV cell
ÄKTAprime plus Operating Instructions 28-9597-89 AB
Corrective action
Clean the system. Make sure air is
removed.
Clean the column according to the
column instructions.
Check the mixer function by plac-
ing a stirrer bar on top of the mixer
housing. The stirrer bar should ro-
tate when the system is in Run
mode. The mixer function can also
be checked by running the installa-
tion test.
Clean the UV cell by flushing
Decon™ 90, Deconex™ 11 or
equivalent.
Check if the signal is still noisy with
water.
Purge the pump according to
Pump User Manual. Run a system
wash with buffer.