A Appendix; Glossary - GE ImageQuant LAS 4000 User Manual

Appendix A Appendix
A.1 Glossary
CCD Calibration
Electric charges are accumulated on the CCD even if it is not exposed to light. The accumulated electric
charge is called a dark current noise. The process for eliminating the noise component is performed
by CCD calibration. The image constituted by the dark current noise is called CCD calibration data. An
image that is constituted by only the electric charges accumulated during exposure can be created
by subtracting the CCD calibration data from the exposed image.
Flat Frame
In an exposure system using a 2D sensor such as a CCD, unevenness due to the lens or lighting system
appears on the image. By correcting the unevenness, an object that radiates light at the same brightness
is represented evenly in any section of an exposure area. A correction file for correcting unevenness
is called a flat frame. Under the same conditions (tray position, iris, fluorescent filter, light source, and
tray) as when exposing an object sample, a flat frame records the image in a uniform fluorescent plate
(fluorescent mode) or white plate (digitize mode).
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