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4.2 Measuring Operation
4.2.3 Preparatory Operations of Autosampler Proper
(1)
Check that the autosampler is correctly connected to the
pump and detector before turning on the power.
(2)
Check that the drain tube is correctly connected to the waste
(solution) bottle. The waste bottle must be positioned at a
level lower than the autosampler.
(3)
Prepare other components to be connected to the
autosampler, referring to the respective instruction manual.
(4)
The drain tube of the detector must be placed in a waste
bottle that is dedicated for the detector.
(5)
Connect the mobile phase bottle to the pump and connect the
detergent bottle to the autosampler.
(6)
Start the pump to fill the flow paths in the pump and
autosampler with liquid. Before a new connection to the
column, fill the flow path with the mobile phase, and then
connect a tube in order to prevent air bubbles from entering
the column. And connect the column after making sure the
tube up to the column is filled with the mobile phase.
(7)
After the mobile phase has reached the injection valve of the
autosampler, turn on power supply to the autosampler. Avoid
switching the injection valve before the mobile phase reaches
it, otherwise the valve seal might be damaged.
Start maintenance software and click Purge button in
(8)
"Washing Pump Purge" to fill the flow path to the syringe
valve with wash fluid. (When two wash fluids are used,
replace the wash fluid2 side with fresh one). Then, make sure
that there are no air bubbles in the pipe from the wash pump
to the syringe valve.
Click Purge button in "Syringe Purge" of maintenance
(9)
software to fill the syringe with wash fluid. Then, make sure
that there are no air bubbles in the syringe and port4 of the
syringe valve discharges waste solution.
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