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Sartorius Biostat RM 20 Operating Instructions Manual
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Sartorius Biostat RM 20 Operating Instructions Manual

Sartorius Biostat RM 20 Operating Instructions Manual

Bioreactor prepared for biobrain
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Operating Instructions
Translation of the Original Operating Instructions
Biostat
RM 20 | 50
®
Bioreactor prepared for Biobrain
®
2882500-000-01

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Summary of Contents for Sartorius Biostat RM 20

  • Page 1 Operating Instructions Translation of the Original Operating Instructions Biostat RM 20 | 50 ® Bioreactor prepared for Biobrain ® 2882500-000-01...

  • Page 3: Table Of Contents

    Contents Contents 1 About This Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7 Scope.
  • Page 4 Contents 3.7 Heating Mats ..............21 3.8 Temperature Sensor .
  • Page 5 Contents 6.2.10 Settings | “Settings” Menu ..........48 6.2.10.1 Setting the Time and Date .
  • Page 6 15 Sartorius Service . . . . . . . . . . . . . . . . . . .

  • Page 7: About This Document

    About This Document About This Document Scope These instructions are part of the device. These instructions apply to the device in the following versions: Device Type Biostat RM 20 | 50 DHR050LBRM ® Connected to the control tower or basic design Optionally with Culture bag holder 20 DZ020L-R2BH...

  • Page 8: Symbols Used

    About This Document Target Group Knowledge | Responsibilities Administrator The administrator is responsible for integrating the device into the production process. The administrator ensures the reliable functioning of the system and device software. The administrator is trained in the operation of the device. Operator The device operator is responsible for ensuring compliance with workplace health and safety...

  • Page 9: Safety Instructions

    (see Chapter “1.3 Target Groups,” page 7). The actions described are addressed to the user. If individual actions must be carried out by other target groups or by Sartorius Service personnel, the required qualification will be indicated. General Safety Instructions Do not start the device if other people are in the danger zone.

  • Page 10: Significance Of These Instructions

    Make sure that the power connection is equipped with a ground lead. Never open the control cabinet. The device may only be opened by Sartorius Service personnel. Work on the electrical equipment of the device may only be carried out by Sartorius Service.

  • Page 11: Unsuitable Power Supply Cables

    Please use only the original power cable supplied to connect the device to the power supply socket. If the power supply cable is damaged, please contact Sartorius Service. Danger due to Gasses 2.8.1 Danger of Fire due to Pure Oxygen (O Pure oxygen can give raise to chemical reactions that could cause substances to self-combust.

  • Page 12: Accessories, Consumables, And Spare Parts

    — Damage to the device — Malfunctions of the device — Device failure Only use accessories, consumables, and spare parts supplied by Sartorius. Only use accessories, consumables, and spare parts that are in proper working condition. 2.10 Emergency Instructions If there is an immediate danger of personal injury or equipment damage, e.g., due to malfunctions or dangerous situations, take the device out of...

  • Page 13: Danger Due To Leaked Substances

    Safety Instructions Wear suitable personal protective equipment. Also follow any instructions posted in the work area pertaining to personal protective equipment. 2.12 Danger due to Leaked Substances 2.12.1 Danger of Scalding due to Defective Components Defective components can cause hot media or substances to leak. Hot media can burn and cause personal injury.

  • Page 14: Risk Of Death From Energy That Is Unexpectedly Released

    There is a risk of impermissible pressurization breaking the culture bag when using aeration devices from third-party manufacturers. Only use the integrated aeration module (optional) or a Sartorius control unit, since these have an overpressure relief valve. Use the filter heaters during cultivation when the device is operated.

  • Page 15: Moving Parts

    Safety Instructions 2.16 Moving Parts There is a risk of limbs being crushed if personnel come into contact with the rocker while the device is in operation. Do not remove the safety mechanisms. Allow only qualified and authorized personnel to work on the device. Disconnect the device from power when performing maintenance and cleaning tasks.

  • Page 16: Device Description

    Device Description Device Description Device Overview Fig. 1: Overview of the Biostat RM 20 | 50 (example) ® No . Designation Filter heater Sterile filter for exhaust air Sterile filter for supply air Hood Culture bag Culture bag holder Module with rocker Control unit Connections, right side Load cell module (weighing device)
  • Page 17 Device Description Intended Use of the Device The Biostat RM 20 | 50 is a rocker bioreactor platform for single-use ® culture bags. The platform is also referred to as a “rocker” in these instructions. The shaft mixing process of this platform uses mechanical energy to ensure homogenous mixing of cells.

  • Page 18: Connections

    Device Description Connections 3.2.1 Rear Connections Fig. 2: Biostat RM 20 | 50 connections, rear side ® No . Designation Description Manufacturer’s ID label D-LINK 1 Ethernet port (Connection to Biostat ® control unit) SIG 2 Potential-free alarm output SIG 3 Serial interface to the load cell module, RS-232 connection D-LINK 2...

  • Page 19: Load Cell Module Connections

    Device Description 3.2.2 Load Cell Module Connections Fig. 3: Load cell module connections, rear side No . Designation Description RS-232 Serial interface for the load cell module Mains connection for Power supply connection for the load the load cell module cell module Manufacturer’s ID label 3.2.3...

  • Page 20: Load Cell Module (Optional)

    Device Description Load Cell Module (Optional) The RM 20 | 50 load cell module is an optional weighing platform for the Biostat RM 20 | 50, which is used to weigh the culture medium during the ® process. The weighing platform is added to the Biostat RM 20 | 50 base ®...

  • Page 21: Aeration Module (Optional)

    Device Description Aeration Module (Optional) Air and CO Supply Mixture control using the CO concentration controller (For control ranges, see Chapter “13.8.2 Aeration and Ventilation,” page 79). For the air intake, you can select either an integrated air pump that supplies the culture bag with room air or a compressed air supply on the rear side of the rocker housing for external air intake.

  • Page 22: Temperature Sensor

    Device Description Temperature Sensor The temperature sensor is a surface temperature sensor and measures the temperature of the culture bag. The temperature sensor is run through the opening into the culture bag holder and connected to the device via a plug connection. The head of the temperature sensor is fixed in the opening of the culture bag holder using an O-ring.

  • Page 23: Safety Equipment

    Device Description The following cultivation parameters can be set and viewed on the display: — Rocker speed and angle — Aeration — Temperature 3.11 Safety Equipment 3.11.1 Main Switch The main switch (1) serves as a switch disconnector. The device is switched on and off via the main switch.

  • Page 24: Temperature Control

    Device Description 3.13 Temperature Control The device comes with a dual temperature measurement and control system as standard. If the device is connected to a Biostat RM control unit: only the left ® temperature measurement can be used. The heating mats can be used to heat culture bags.

  • Page 25: Setup

    Setup Setup Acclimatization Condensation from humidity can form on the surfaces of a cold device when it is brought into a substantially warmer area. You should therefore allow a device that has been disconnected from its power source to acclimatize for approximately two hours before reconnecting it to the power.

  • Page 26: Unpacking The Device

    ID labels (For type plate position, see Chapter “3.1 Device Overview,“ page 16). When corrosive gasses are used, check whether the installed fittings are suitable, e.g., stainless steel fittings. Please contact Sartorius Service in case of any questions concerning equipment. 4.4.2...

  • Page 27: Gas Supply

    Ensure that the power supply cable is suitable for the device output and voltage and the mains connection at the installation location. If required: contact Sartorius Service. Do not repair defective mains cables or replace incorrect connectors. Please contact Sartorius Service.
  • Page 28 Setup Procedure NOTICE Risk of malfunctions and damage to gas-carrying components due to unsuitable gasses! Ensure that all gas-carrying components are corrosion-resistant if corrosive gasses are used in the supply. Gas-carrying components made of brass may corrode when exposed, for example, to ammonia. Check whether the gas supply at the installation location meets the requirements (see Chapter “13.8.2 Aeration and Ventilation”, page 79).

  • Page 29: Getting Started

    Installation Kit The scope of delivery includes the required connections and connecting pieces. Only use the connections and accessory parts supplied and approved by Sartorius. Only replace connections and accessory parts with accessories from Sartorius. Installing the Culture Bag Holder 5.2.1...

  • Page 30: Installing The Temperature Sensors

    Getting Started Connect the cable of the left heating mat to connection H1 (1) and the cable of the right heating mat to connection H2 (2). Installing the Temperature Sensors 5.3.1 Temperature Sensor in Connection with Heating Mats If the temperature sensors are positioned incorrectly, this can lead to overheating of the heating mats.

  • Page 31: Connecting The Compressed Air And Co

    (see Chapter “4.4.2 Power Supply,” page 26). If the input voltage is too high: Do not connect the device to the power supply. Contact Sartorius Service. Insert the power supply cable plug into the “Mains Connection” (1) on the back of the device.

  • Page 32: Switching The Device On And Off

    Getting Started Switching the Device On and Off CAUTION Danger of crushed limbs by the rocker! Making adjustments between the culture bag holder and the rocker during operation may result in hand injuries. Allow only qualified and authorized personnel to work on the device. Procedure Make sure that the device has been set up in accordance with the steps listed above and that all components have been properly installed.

  • Page 33: Connecting The Device To Decentralized Control Systems (Dcs)

    Getting Started Connecting the Device to Decentralized Control Systems (DCS) 5.6.1 Options for Connecting the Device to Other Devices Fig. 5: Overview of options for connecting the device to other devices Operating Instructions Biostat RM 20 | 50 ®...

  • Page 34: Options For Connecting To Deltav Systems

    Getting Started No . Designation Description Omron Host Link Flow modules Connection optional Profibus connection Connection optional D-LINK 1 Connection socket for RJ45 plug D-LINK 2 Connection socket for D-SUB 9-pin plug SIG 3 Connection socket for Lemo 4-pin plug D-LINK 3 Connection socket for D-SUB 9-pin plug Profibus DP...
  • Page 35 Getting Started Fig. 6: Overview of options for connecting to DeltaV systems No . Designation Description Laboratory Device installation location Profibus Connection optional Process Process area Driver Provided on site (not included with the equipment supplied) Office Data collection and analysis Operating Instructions Biostat RM 20 | 50 ®...

  • Page 36: Changing The Communication Settings

    Getting Started 5.6.3 Changing the Communication Settings Basic functions of the device can be adjusted in the communication settings. Requirements You have “supervisor” user authorization (For authorizations, see Chapter “6.2.8 User Management | “User level” Menu,” page 46). Procedure Press the home button in the footer. The “Main Screen”...

  • Page 37: Connecting The Device To Ethernet

    Getting Started 5.6.4 Connecting the Device to Ethernet Procedure NOTICE Damage to the device or malfunctions due to incorrect con- nection! Observe the information on the communication interfaces (see Chapter “13.12.1 D-LINK 1: Ethernet,” page 81). Connect the Ethernet cable to the [D-LINK 1] (Ethernet) port. We recommend using an OPC server including Omron FINS Ethernet protocol.

  • Page 38: Operation

    Operation Operation Process Sequence Overview The process sequence and the operation of the device involves the following steps: Process Sequence Steps Chapter, Page Installing the culture bag on the culture bag holder 6.3.1, 54 Installing the filter heater 6.3.2, 54 Connecting the air supply 6.3.3, 55 Inflating the culture bag...

  • Page 39: Main Screen" Menu

    Operation 6.2.1 “Main Screen” Menu The “Main Screen” menu is the central user interface from which the submenus and footer menus can be selected. The “Main Screen” menu may take up several pages depending on the equipment. Procedure Press the [Next] button to access the next page of the “Main Screen” menu.

  • Page 40: Overview" Menu

    Operation 6.2.2 “Overview” Menu The “Overview” menu displays the most important parameters as actual values. A green dot next to the nominal values indicates whether or not the respective controller has been started. The asterisk (*) in the figures shown here acts as a placeholder for the actual value.

  • Page 41: Aeration With Co ("Co " Menu)

    Operation 6.2.3.2 Aeration with CO (“CO ” Menu) Procedure Press the [SET] button to enter the setpoint. (For setpoint, see Chapter “13.8.2 Aeration and Ventilation,” page 79). The % display of CO is the amount in the entire gas flow. “CO Trend”...

  • Page 42: Rocker Speed, Rocker Angle | "Speed" And "Angle" Menus

    Operation 6.2.4 Rocker Speed, Rocker Angle | “SPEED” and “ANGLE” Menus The “rocking process” can be started and stopped in both menus. The controller regulates the rocking rate as well as the angle for the desired setpoints. One rock (rocker movement) corresponds to one movement from the center to the front position –...

  • Page 43: Temperature | "Temp 1" And "Temp 2" Menus

    Operation 6.2.5 Temperature | “TEMP 1” and “TEMP 2” Menus There are two heating circuits on the reactor that can be controlled independently depending on the bag configuration (see Chapter “6.2.10.3 Setting the Culture Bag Size,” page 50). Procedure Press the [SET] button to enter the setpoint. (For control range, see Chapter “13.9.3 Temperature Control,”...

  • Page 44: Weighing Function | "Loadcell" Menu

    Operation Procedure Press the [Start pos process] button to activate the function. Press the [SET] button to configure the setpoint. Select the direction in which the tray should be moved. 6.2.7 Weighing Function | “LOADCELL” Menu Procedure Press the [LOADCELL] button in the “Main Screen” menu to access the “Loadcell”...

  • Page 45: Loadcell Zero" Submenu

    Operation 6.2.7.1 “Loadcell Zero” Submenu Procedure Run the zero point function only at the beginning of the process. Note that overwritten zero point values cannot be restored. Holding down the [Zero] button for longer than three seconds allows the user to enter the zero point manually. If the zero point is entered manually, the “History”...

  • Page 46: User Management | "User Level" Menu

    Operation Switching between the Gross or Net Weight Display Procedure Press the [Fn Function] button in the “Loadcell” submenu to switch between the gross and net weight displays. 6.2.8 User Management | “User level” Menu The user status (“User-level:”) is displayed in the header. If the user status is “locked,”...
  • Page 47 Operation The keypad appears. Enter the password and confirm the entry with [OK]. — You can enter up to eight characters. — The default passwords in the factory settings are: — User: SART02 — Supervisor: SART01 The system automatically switches to the respective user status depending on the password.

  • Page 48: Alarms | "Alarm" Menu

    Operation 6.2.9 Alarms | “Alarm” Menu The window with alarm messages can be accessed from the “Main Screen” menu. This screen is displayed automatically by the system when an alarm or message occurs. You can view the alarm list even if no alarms have been triggered by the system.

  • Page 49: Displaying Service Information

    Operation Automatic Adjustment for Daylight Saving Time You can set whether the clock is automatically adjusted by one hour for daylight saving time on the last Sunday in March and the last Sunday in October in the “Automatically Summer-/Wintertime change:” field. The device must be in operation at the time of the adjustment, because the clock will not adjust retrospectively.

  • Page 50: Setting The Culture Bag Size

    Operation 6.2.10.3 Setting the Culture Bag Size Requirements Both Pt100 temperature sensors are installed. Procedure Press the [Bag configuration] button in the “Settings” menu to set the bag configuration. The following configurations are available: Configuration Description 1 x 1 L, —...

  • Page 51: Selecting The Control Mode

    Operation 6.2.10.4 Selecting the Control Mode Procedure Press the [Control mode] button in the “Settings” menu. The “Control mode” display appears. The following settings can be configured for the control mode: Input Description Display The device is selected as the master. All alarms and warnings are shown on the device display.
  • Page 52 Operation Press the [Local] button to activate remote control for the device. The device switches to the next display. The following information is displayed: — CPU firmware version — Display configuration — Bag configuration — Connection status. The connection status is displayed with the symbol “ ...

  • Page 53: Setting The Controller Behavior After A Restart

    Operation 6.2.10.5 Setting the Controller Behavior after a Restart Access to the device and the device behavior after a restart can be configured: Procedure To define the start-up behavior for the controller after a restart: Press the [System] key in the “Settings” menu. The “System settings”...

  • Page 54: Preparing The Culture Bag

    Operation Preparing the Culture Bag 6.3.1 Installing Culture Bags on the Culture Bag Holder One culture bag can be inserted in the culture bag holder depending on its size. (Detailed information on the size of the culture bags that may be installed on the rocker can be found in Chapter “13.4 Culture Bag Positioning and Operating Volumes,”...

  • Page 55: Connecting The Air Supply

    Bursting culture bags can cause media to escape and lead to injury due to chemical burns. Connect the air supply only to the connections on the device. Only use the integrated aeration module or a Sartorius control unit, since these have an overpressure relief valve. Procedure Connect the supplied tube to connection “GAS 1”...

  • Page 56: Inflating The Culture Bag

    Operation 6.3.4 Inflating the Culture Bag The culture bag should be inflated before it is filled with the culture medium. This reduces the foaming of the medium. Regulate the air intake so that the culture bag remains inflated during filling and does not collapse.

  • Page 57: Setting The Cultivation Parameters And Starting Cultivation

    Operation 6.3.6 Setting the Cultivation Parameters and Starting Cultivation If the foam build-up is too high, the rocking speed must be reduced. The culture bag must be fully inflated, since the formation of foam increases due to folds or unevenness. Recommendations for Rocker Speed, Rocker Angle, and Aeration Rate Air intake (aeration rate) and CO concentration...

  • Page 58: Sampling And Inoculation

    Operation Setting the Cultivation Parameters and Starting Cultivation Requirements The device is switched on. NOTICE Risk of overheating due to no contact between the temperature sensor and the culture bag! There is a risk of overheating if the temperature sensor is not correctly installed.

  • Page 59: Inoculation

    Operation Disinfect the Luer septum. Wipe or spray the upper part of the Luer septum with 70% alcohol (or similar medium). Connect a sterile, single-use syringe without a needle to the Luer connection under aseptic conditions. Open the tube clamp of the Luer septum. 6.4.2 Inoculation Procedure...

  • Page 60: Sampling

    Operation 6.4.3 Sampling Sampling is carried out in exactly the same way as inoculation; a syringe without a needle is also used here. However, you must make sure that the cells which are inside the tubing are removed before taking your representative sample. The Luer septum is designed for 50 sterile sampling and | or inoculation procedures.

  • Page 61: Emptying The Culture Bag And Cell Harvesting

    Operation 6.5.2 Emptying the Culture Bag and Cell Harvesting We recommend emptying the culture bag completely before removal. Procedure Remove the hood from the culture bag holder. Clamp off the intake air and exhaust filters. Remove the air intake tubing from the sterile intake air filter. There are several options available for emptying the culture bag, depending on the process conditions: —...

  • Page 62: Cleaning And Maintenance

    In case of defects, turn the power supply off immediately and have the defects corrected by Sartorius Service. Have the electrical components and stationary electrical equipment checked by an electrician at least every four years.

  • Page 63: Preliminary Steps

    Cleaning and Maintenance Preliminary Steps Make sure to always perform the following preliminary steps during cleaning and maintenance. Procedure Turn the device off at the main switch. Remove the power supply from the laboratory connection. Turn off all supply media in the lab (water and gas supply). Ensure that the connections and hoses have been depressurized.

  • Page 64: Maintenance

    However, we recommend that it be replaced within this five-year period. This service is included within the scope of maintenance, if necessary. For more information on maintenance work, contact Sartorius Service. Operating Instructions Biostat RM 20 | 50...

  • Page 65: Faults

    Inform management on site about the fault. Determine the cause of the fault and remedy it before switching the device back on. If the fault cannot be remedied, please consult Sartorius Service. Operating Instructions Biostat RM 20 | 50 ®...

  • Page 66: Hardware-Related Faults

    Faults Hardware-Related Faults WARNING Danger of injury if qualifications are insufficient! Improper use can lead to significant personal injury and property damage. It is important that all troubleshooting activities be carried out by qualified personnel. 8.2.1 Contamination Troubleshooting Table We recommend carrying out a sterility test before each process for a duration of 24 – 48 h. Conditions for a sterility test: —...

  • Page 67: Temperature Control" Troubleshooting Table

    — Make sure that the culture bag is in motion. If the temperature controller is still not working properly, recalibration of the temperature measurement may be necessary. Contact Sartorius Service about this problem. The temperature seems to be The temperature —...

  • Page 68: Aeration And Ventilation" Troubleshooting Table

    — Close the tube clamp of the blocked exhaust air filter. — Install the filter heater on the second exhaust air filter and open its tube clamp. Please contact Sartorius Service if you have any further questions. Operating Instructions Biostat RM 20 | 50 ®...

  • Page 69: Alarm Messages

    Faults Alarm Messages The Biostat RM 20 | 50 displays alarm messages on the screen to inform the user of various malfunctions. ® Contact Sartorius Service in case of doubt. Alarm Message Potential Causes Measures Battery low This is displayed when the PLC backup —...
  • Page 70 Faults Alarm Message Potential Causes Measures No CO If the CO controller is active (requires that — Please check whether or not the the Gasflow1 controller is also active), this connection tubing to the device has been alarm message is displayed after five crushed or kinked.
  • Page 71 This does not deactivate the controller. connection tubing to the device has been crushed or kinked. — When operating with an internal air pump – please contact Sartorius Service. — Press the Home button to acknowledge this alarm. No Airflow2 If the Gasflow2 controller is active and no —...

  • Page 72: Decommissioning

    Decommissioning Decommissioning Decommissioning the Device Requirements The process has ended. There is no culture bag inserted in the culture bag holder. Procedure Disconnect the compressed air supply. Clear the culture bag, pipelines, and hoses of all culture media lines and additives.

  • Page 73: Storage And Shipping

    Defective devices or parts can be returned to Sartorius. Returned devices must be clean, decontaminated, and properly packed. Transport damage as well as measures for subsequent cleaning and disinfection of the device or parts shall be charged to sender by Sartorius. WARNING Risk of injury due to contaminated devices! Devices contaminated with hazardous materials (ABC contamination) will not be accepted for repair or disposal.

  • Page 74: Disposal

    Disposal 12 Disposal 12.1 Information on Decontamination The device does not contain any hazardous materials that would necessitate special disposal measures. The cultures and media (e.g., acids, bases) used during the fermentation process are potentially hazardous materials that could cause biological or chemical hazards.

  • Page 75: Technical Data

    Technical Data 13 Technical Data 13.1 Dimensions Unit Value Device (width | depth | height) Biostat RM 20 with bag holder 20, hood 765 | 613 | 500 ® Biostat RM 20 with bag holder 20, hood, Loadcell RM 20 765 | 662 | 562 ®...

  • Page 76: Device Components

    Technical Data 13.3 Device Components 13.3.1 Heating Mat Unit Value Article number DZ020L-R2HP Power supply Power consumption Overheating protection: bimetal trip Trip temperature for overheating protection °C +70 ± 5 13.3.2 Temperature Sensor Unit Value Article number DZ-----R2PT Type: resistance thermometer Pt100, class A (EN 60751), three-phase Protection class IP67 Material...

  • Page 77: Culture Bag Positioning And Operating Volumes

    Technical Data Unit Value Static (with centered medium load) ± 10 Static (with uncentered lateral load) ± 30 Dynamic ± 50 Material Frame: stainless steel Housing: ABS (UL-compliant) Interfaces Data Power supply: external power supply with country-specific adapters RS-232 Electrical characteristics Input voltage 100 –...

  • Page 78: Approved Culture Bags

    Technical Data 13.5 Approved Culture Bags Type Unit Gross volume of the culture Flexsafe RM basic 1 – 50 ® Flexsafe RM optical 2 – 50 ® Flexsafe RM perfusion 2 – 50 ® Flexsafe RM Viamass 2 – 50 ®...

  • Page 79: Aeration And Ventilation

    Technical Data 13.8.2 Aeration and Ventilation Unit Value Room air A membrane pump compresses the room air at the rear “AIR” compressed air connection* Compressed air The integrated pressure regulator must be connected to an external 1.5 ± 0.2 compressed air source* Quick coupling for the direct connection of a rigid tube, external diameter 4.0 ±...

  • Page 80: Aeration And Ventilation

    Technical Data 13.9.2 Aeration and Ventilation Unit Value Aeration rate (mass flow controller for flow speeds)* ml / min 50 – 1,000 Reading accuracy ±5 Max. aeration rate per control circuit (flow circuit): 1 flow circuit active ml / min 1000 2 flow circuits active ml / min...

  • Page 81: Acoustic Emissions

    Technical Data 13.11 Acoustic Emissions Unit Value Device switched on, idle, aeration off dB (A) Device switched on, 42 RPM, aeration off dB (A) Device switched on, idle, aeration on dB (A) Device switched on, 42 RPM, aeration on dB (A) 13.12 Communication Interfaces 13.12.1 D-LINK 1: Ethernet Interface Specification...

  • Page 82: D-Link 2: Rs-232 Serial Interface

    Technical Data 13.12.2 D-LINK 2: RS-232 Serial Interface D-SUB Connector PIN no . Abbreviation Signal Name Shield Send data Output Receive data Input RTS (RS) Request to send Output CTS (CS) Clear to send Input Power supply DSR (DR) Data set ready Input DTR (ER) Data terminal ready...
  • Page 83 Technical Data Modbus RTU Unit Value Type: RS-232 Transmission rate Baud 57600 Data bits Parity control: Even Stop bits Connector: D-SUB Protocol: Modbus RTU slave mode Slave address: 10 Supported commands 0 x 03 Read Holding Registers: Reads multiple registers from the DM or EM area of the I/O memory at the same time.

  • Page 84: D-Link 3: Profibus Dp

    Technical Data 13.12.3 D-LINK 3: Profibus DP Interface Specification Unit Value Address: 2 (factory setting; cannot be changed) Connector: D-SUB 13.12.4 Data Table Name | Description Type Scaling Range Address Factor UNIT: 16 bits | 2 bytes System data (read-only) Serial number D7000 DWORD...
  • Page 85 Technical Data Name | Description Type Scaling Range Address Factor Process values (read-only) Setpoint for rocker speed D7020 UINT 8 – 42 rocks/min Current rocker speed D7021 UINT 8 – 42 rocks/min Setpoint for rocker angle D7022 UINT 4.0 – 10.0° Current rocker angle D7023 UINT...
  • Page 86 Technical Data Name | Description Type Scaling Range Address Factor Error status (read-only) Battery weak D7052.00 BOOL Reserve D7052.01 BOOL Load cell module overload D7052.02 BOOL Pt100_1 error D7052.03 BOOL Pt100_2 error D7052.04 BOOL Heater 1 error D7052.05 BOOL Heater 2 error D7052.06 BOOL Drive system error...
  • Page 87 Technical Data Name | Description Type Scaling Range Address Factor Commands (write-only) Rocker remote control “alive” bit D7070.00 BOOL 1 s pulse signal Start rocker function D7070.01 BOOL Start heating mat 1 D7070.02 BOOL 1 = Starts Start heating mat 2 D7070.03 BOOL 0 = Stops...

  • Page 88: Accessories And Consumables

    Accessories and Consumables 14 Accessories and Consumables 14.1 Accessories This table contains an excerpt of accessories that can be ordered. For information on other products, contact Sartorius. Item Quantity Order Number Filter heater for standard HEPA filter DZ-----R2FH Sartopore Air Midisart...
  • Page 89 Accessories and Consumables Item Quantity Order Number Flexsafe RM perfusion culture bags ® Flexsafe RM 2L perfusion DFP002L--SM ® Flexsafe RM 10L perfusion DFP010L--SM ® Flexsafe RM 20L perfusion DFP020L--SM ® Flexsafe RM 50L perfusion DFP050L--SM ® Flexsafe RM 2L perfusion ATF DFP002L--AT ®...

  • Page 90: Sartorius Service

    Sartorius Service 15 Sartorius Service Sartorius Service is at your disposal for queries regarding the device. For in- formation about the service addresses, services provided, or to contact a local representative, please visit the Sartorius website (www.sartorius.com). When contacting Sartorius Service with questions about the system or in the event of malfunctions, be sure to have the device information, e.g.
  • Page 91 Licensing Information Operating Instructions Biostat RM 20 | 50 ®...
  • Page 92 Licensing Information Operating Instructions Biostat RM 20 | 50 ®...
  • Page 93 Licensing Information Operating Instructions Biostat RM 20 | 50 ®...
  • Page 94 Licensing Information Operating Instructions Biostat RM 20 | 50 ®...

  • Page 95: Appendix

    Appendix 18 Appendix 18.1 Aerating the Cell Culture 18.1.1 Schematic Representation of Aeration The Biostat RM 20 | 50 can be used to aerate the cell culture with either ® external compressed air or compressed room air. A pressure sensor monitors the pressure and is equipped with an alarm signal (see Chapter “18.1.2 Over- pressure Control,”...

  • Page 96: Overpressure Control

    Appendix 18.1.2 Overpressure Control A pressure sensor records the pressure of the culture bag. If the pressure rises to more than +30 mbar, the control valve closes automatically until the pressure decreases to +20 mbar. This protects the cell culture from overpressure and protects the culture bag from damage.
  • Page 97 The information and figures contained in these instructions correspond to the version date specified below. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. Masculine or feminine forms are used to facilitate legibility in these instructions and always simultaneously denote all genders.

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