Sartorius PICUS User Manual
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Sartorius PICUS User Manual

Electronic pipette
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Picus® & Picus® NxT Electronic Pipette
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Summary of Contents for Sartorius PICUS

  • Page 1 Picus® & Picus® NxT Electronic Pipette User Manual...

  • Page 2: Table Of Contents

    3.4.6. Reset 3.4.7. Information 3.4.8. Languages 3.4.9. Pipette Lock 3.4.10. Passwords (only in Picus® NxT) 3.4.11. Date and Time (only in Picus® NxT) 3.4.12. Reminders (only in Picus® NxT) 4. Care and Maintenance 4.1. Cleaning and Maintenance 4.1.1. Single-Channel Pipettes 4.1.2.

  • Page 3: Introduction

    Optiload tip-loading feature and the electronic tip ejection function. To improve safety by reducing the risk of contamination, replaceable Safe-Cone Filters can be used in all Picus®/Picus® NxT models greater than 10 µl. The fully electronic operation, unique DC motor, and the electronic brake and piston control system guarantee excellent accuracy and precision.

  • Page 4: Intended Use

    Directive 98/97/EC of the European Parliament. The Picus®/Picus® NxT product range covers a volume range of 0,2 µl to 10 ml. It is recommended that Sartorius Optifit Tips or SafetySpace Filter Tips are used with Sartorius pipettes to ensure optimum compatibility and performance.

  • Page 5: Display

    6. Arrows indicating aspiration and dispensing 1.2.3 Optifit Tips and SafetySpace Filter Tips We recommend using Sartorius Optifit Tips or SafetySpace Filter Tips. These tips are designed for Sartorius pipettes and therefore ensure optimal compatibility, accuracy, and precision. For optimum results you should also: −Ensure that the liquid and the pipette/tip combination are at approximately...

  • Page 6: Contents Of Delivery Package

    −Picus®/Picus® NxT electronic pipette − Universal USB charger (EU, UK, US, JPN, KOR, AUS, and CHN plugs) − Sartorius tip rack (10x96 tips) of corresponding volume with up to 1000 µl single-channel models / 1200 µl multichannel models − One tip with 5 ml and 10 ml single-channel models −...

  • Page 7: Getting Started

    2. Getting Started Please read this manual before using your Picus®/Picus® NxT electronic pipette. 1. Your Picus®/Picus® NxT is delivered, with the battery partially charged. We recommend the pipette is charged fully, before first use; charge at least one (1) hour.

  • Page 8: Operation

    3. Operation 3.1. Operating Principles Pipetting functions are controlled using the operating button, adjustment wheel, and Softkeys. The Hotkey is used to store or activate programs. Operating button −Confirms settings (can be used as an alternative to the Softkey for OK) and initiates piston movement for aspiration, dispensing and repeated blow-out.

  • Page 9: Pipetting Modes

    3.2. Pipetting Modes The Picus® NxT electronic pipette has nine (9) main pipetting modes, while the Picus® has eight (8). The Picus® NxT has seven (7) advanced functions and the Picus® has six (6). Pipetting Modes Advanced Functions (ADV)* Tracker...

  • Page 10: Pipetting (Forward Pipetting)

    Advanced Functions Counter, Mixing, and Tracker can be used in conjunction with Pipetting mode. In Picus® NxT, Repeated Blow-out can also be used. Repeated Blow-out helps to remove residual liquid from the tip, for liquids where normal dispensing does not empty it sufficiently.

  • Page 11: Multi-Dispensing

    2. Turn the adjustment wheel to set the desired value. 3. Confirm the setting by pressing a. the operating button or the right Softkey for OK, which will exit the editing mode. b. the middle Softkey for NEXT, to move on to edit the next highlighted setting.

  • Page 12: Manual Pipetting

    3. Press the operating button repeatedly until all aliquots have been dispensed. 4. To continue repetitive dispensing without discarding the excess volume, press the left Softkey for NO and return to step 1. 5. To discard the excess volume and to empty the tip, press the operating button twice.

  • Page 13: Diluting

    5. Empty the tip by pressing the operating button again. 6. Eject the tip by pressing the electronic tip ejector button. Advanced Functions Repeated Blow-out (Picus® NxT only) and Mixing can be used in conjunction with Diluting. −Mixing mixes liquids manually or automatically. The mixing volume can be adjusted according to the pipette’s maximum volume.

  • Page 14: Multi-Aspirating

    5. Eject the tip by pressing the electronic tip ejector button. Advanced Functions Repeated Blow-out (Picus® NxT only) and Excess Adjustment can be used in conjunction with Sequential Dispensing. −Excess Adjustment can be used to set the excess volume.

  • Page 15: Titration

    2. To dispense the liquid and empty the tip, press the operating button again. 3. Eject the tip by pressing the electronic tip ejector button. Advanced Functions Repeated Blow-out, available with Picus® NxT, can be used in conjunction with Multi-Aspiration mode, for liquids where normal dispensing does not completely empty the tip.

  • Page 16: Protocols (Only In Picus® Nxt)

    3.2.9. Protocols (Only in Picus® NxT) User-definable protocols speed up routine pipetting sequences. A maximum of ten pipetting and advanced funtion combinations can be used within a protocol. For example, pipetting with mixing counts as one combination. To set up or edit a pipetting protocol: 1.

  • Page 17: Advanced Functions

    8. To remove a step: a. Use the adjustment wheel to select Delete step and confirm with the operating button or the Softkey for OK. The pipette then lists the available steps. b. Use the adjustment wheel to select the step to be deleted and confirm with the operating button or the Softkey for OK.
  • Page 18 Not all advanced functions can be used together. Tracker, Mixing, Auto Dispensing, and Counter cannot be active at the same time, but Repeated Blow-out (only in Picus® NxT) can be used together with all other advanced functions. Excess Volume Adjustment doesn’t affect other advanced functions.
  • Page 19 Counter Counter counts the number of pipetting cycles up to 384 and can be used in conjunction with Pipetting and Reverse Pipetting modes. You can start counting from any number up to 384. The starting number can be set with the adjustment wheel directly after the advanced function has been activated, or using the EDIT function in the main mode.

  • Page 20: Saving Pipetting Programs To Memory

    3.3. Saving Pipetting Programs to Memory All Picus® and Picus® NxT models allow 10 programs to be saved in the pipette memory. In the Picus® NxT, sequences of pipetting modes can be stored as protocols (see section 3.2.9).

  • Page 21: Setup

    (see section 3.4.10). 3.4.1. Adjustment The default length of the piston stroke in Picus® and Picus® NxT pipettes is defined by the manufacturer. This factory setting should be used in normal circumstances: with aqueous liquids, at standard atmospheric pressure, and with the pipette, tip, and liquid at standard room temperature.
  • Page 22 To change the accuracy adjustment settings: 1. If password protection is enabled, log in using the administrator profile, (only in Picus® NxT). 2. Select MENU by pressing the left Softkey. 3. Use the adjustment wheel to select Setup and confirm by pressing the operating button or the Softkey for OK.

  • Page 23: Sound

    To reset the pipette to factory adjustment settings: 1. If password protection is enabled, log in using the administrator profile (only in Picus® NxT). 2. Select MENU by pressing the left Softkey. 3. Use the adjustment wheel to select Setup and confirm by pressing the operating button or the Softkey for OK.

  • Page 24: User Id

    In Picus® NxT pipettes, service, calibration, and check reminders can all be programmed. Please see section 3.4.12. To change the dates: 1.

  • Page 25: Reset

    To reset pipette settings: 1. If password protection is enabled, log in using the administrator profile, (only in Picus® NxT). 2. Select MENU by pressing the left Softkey. 3. Use the adjustment wheel to select Setup and confirm by pressing the operating button or the Softkey for OK.

  • Page 26: Pipette Lock

    3.4.10. Passwords (only in Picus® NxT) Picus® NxT pipette has two-level password protection. The first level is administrator level, with unrestricted permissions, and the second is the user level, which is restricted. In password settings, password protection can be activated, deactivated, and both passwords set.

  • Page 27: Date And Time (Only In Picus® Nxt)

    3.4.11. Date and Time (only in Picus® NxT) The Picus® NxT pipette has a calendar and clock that are used for reminders and timers. The date and time are asked for during the initial setup of the pipette, but can also be modified later.

  • Page 28: Reminders (Only In Picus® Nxt)

    3.4.12. Reminders (only in Picus® NxT) Picus® NxT has built in reminders for Maintenance, Calibration, and Pipette checking. The reminders help the user to remember important service dates. Having the pipettes maintained and calibrated at regular intervals is an important factor in ensuring the quality and accuracy of results.
  • Page 29 To turn off a reminder: 1. If password protection is enabled, log in using the administrator profile. 2. Select MENU by pressing the left Softkey. 3. Use the adjustment wheel to select Setup and confirm by pressing the operating button or the Softkey for OK. 4.

  • Page 30: Care And Maintenance

    To avoid pipette and sample contamination and to extend the pipette’s lifetime, Sartorius Safe-Cone Filters (available for pipettes >10 µl) or Sartorius SafetySpace Filter Tips should be used. Sartorius provides service, maintenance, and calibration services through its global network of service centres.
  • Page 31 Parts and materials: 1. Tip ejector collar (polyvinylidenefluoride (PVDF)) in 10 µl, 120 µl, 300 µl and 1000 µl pipettes, (polypropylene (PP)) in 5000µl and 10ml pipettes. 2. Locking ring (polyetherimide (PEI) in 10 ml pipettes, polyamide (PA) in all other pipettes) 3.
  • Page 32 c. With a 10 ml pipette hold the tip cone cylinder firmly and turn the locking ring (2) clockwise with your fingers (see image below). Do not turn the tip cone (3) as this will break the pipette. 4. Clean the tip ejector collar (1), the tip cone holder, the tip cone cylinder, and the piston (4) with a disinfectant liquid or mild detergent and a soft, lint-free cloth.

  • Page 33: Multichannel Pipettes

    Sartorius service provider. Please contact your nearest Sartorius service provider or distributor. 4.2. Sterilizing The lower parts of Sartorius electronic pipettes can be sterilized by autoclaving, with UV or by using disinfectant or decontaminant liquids such as 70% ethanol, 60% isopropanol, mild detergent, or similar. Always make sure the materials of the pipette and the disinfectant or decontaminant liquid are chemically compatible.
  • Page 34 (ISO 8655-1). NOTE! Sartorius specifications are achieved in strictly controlled conditions (ISO 8655-1). Users should establish acceptable maximum permissible errors based on the field of use and the accuracy requirements placed on the pipette...

  • Page 35: Replacing The Battery

    4.4. Replacing the Battery We recommend that the Picus®/Picus® NxT battery is replaced only by an authorized Sartorius service provider. Please contact your nearest Sartorius representative. 4.5. Replacement Parts Sartorius offers a full range of replacement parts. Always contact Sartorius or an authorized representative for original spare parts for your Sartorius pipette.

  • Page 36: Hazardous Substances (Rohs Ii)

    8.2. Troubleshooting Guide Problem Possible Cause Solution Leakage – Incompatible tip – Use original Sartorius tips – Attach the tip firmly – Tip is loose – Clean the pipette – Pipette is dirty – Pipette is broken – Replace the broken parts or send the pipette for service Inaccuracy –...

  • Page 37: Technical Data

    Capacity 3,7 V/350 mAh Charging time Approx. 1 hour Chargers, charging stands USB charger for Picus®/Picus® NxT pipette For indoor and office equipment use only Input voltage 100 – 240 V ~ 50/60 Hz, 160 -180 mA Output voltage 5 V DC, 1400 mA...

  • Page 38: Performance Specifications

    * The listed systematic and random error values are valid for Pipetting mode only and are achieved under strictly controlled conditions during type tests per ISO 8655. Due to Sartorius’ continuous product development, the systematic and random error values may...

  • Page 39: Speed Table

    9.2. Speed Table Speed is measured in Pipetting mode using the maximum volume. In all main pipetting modes, speed can be adjusted separately for aspiration and dispensing. The speed ranges from 1 (slow) to 9 (fast). Single-channel pipettes (speed in seconds) Speed 10 µl 120 µl...

  • Page 40: Ordering Information

    10. Ordering Information Picus® NxT and Picus® Picus® NxT Picus® Channels Volume Range Safe-Cone Filters Optifit Tips* SafetySpace Filter Tips* Order Code Order Code (µl) Standard Plus (µl) (µl) LH-745021 735021 0,2-10  721008 721018 LH-745041 735041 5-120  LH-745061...
  • Page 44 The status of the information, specifications, and illustrations in this manual are subject to change. Sartorius reserves the right to make changes to the technology, features, specifications, and design of the equipment without notice. All trademarks are Sartorius property unless otherwise stated.

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