Cleaning The Lower Part Of The Pipette - Sartorius Proline Plus User Manual

10.2. Cleaning The Lower Part of The
Pipette
If your pipette is in daily use it is recommended to clean|decontaminate
and grease the pipette every three months. It is recommended to send
the multichannel pipettes to your local Sartorius service representative
for cleaning and greasing. To clean and decontaminate the lower parts of
the single channel pipette, follow these steps:
Disassembling and cleaning
(diagrams at the end of the manual)
1. Eject the tip cone filter (if fitted, Chapter 7.3.)
2. Unscrew the tip ejector collar (1) counterclockwise and remove it.
3. Pipettes < 2,000 µl:
Unscrew the tip cone holder (2) counterclockwise and carefully
remove it with the tip cone (3).
Pipettes 2,000 µl, 5,000 µl:
Unscrew the tip cone cylinder (3) counterclockwise and remove it.
Pipette 10,000 µl:
Hold the tip cone cylinder firmly and turn the tip cone holder
clockwise with your fingers.
4. Clean the tip ejector collar, the tip cone holder, the tip cone
(cylinder) and the piston (4) with ethanol (70%), isopropanol (60%) or
mild detergent and soft lint-free cloth.
5. Clean the interior of the tip ejector collar and the tip cone (cylinder)
with a cotton swab. Be careful with the pipettes so that the seal
inside the tip cone will not damage.
6. Rinse the parts with distilled water if needed and let the parts dry.
7. Pipettes 100 µl:
≤
Put a thin layer of grease on the piston (4).
Pipettes 250, 500 and 1,000 µl:
Put a thin layer of grease around the piston seal (4).
Pipettes 2,000, 5,000 and 10,000 µl:
Put a thin layer of grease on the piston seal (4) and the interior of the tip
cone cylinder (3).
Note: Avoid excess grease. Use only the grease provided with the
pipette.
Note: Before reassembling check that no lint or particles are on the
surface of the piston.
Reassembling:
1. Pipettes:
Carefully place the tip cone (3) on the piston and attach it by
screwing the tip cone holder (2) clockwise.
Pipettes 2,000, 5,000 and 10,000 µl:
Carefully place the tip cone cylinder (3) on the piston and screw
clockwise. Make sure that the tip cone (cylinder) is properly
tightened. Avoid over-tightening.
2. Attach the tip ejector collar (1) by screwing it clockwise.
3. Place the new tip cone filter on its place.
4. Press the operating button several times to ensure that the grease
has spread evenly.
5. Check the performance of the pipette.
Note: It is always necessary to check the performance of the pipette
after in-house service or maintenance.
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