GE AKTA GO Operating Instructions Manual page 76

5 Prepare the system for a run
5.4 Connect a column
Step
7
8
9
76
Action
When buffer is dripping from tubing prepared in step 3, fill the top part of
the column with buffer by letting it drip from the tube. When it is filled, con-
nect the tubing drop-to-drop to the top of the column.
Note:
Make sure that the connectors are properly tightened, but do not overtighten
when connecting columns. Overtightening might rupture the connectors or
squeeze the tubing and thereby result in high back pressure.
Cut a piece of tubing with appropriate length to connect the bottom of the
column to the UV monitor. Remove the stop plug from the bottom of the
column and connect this tubing in its place.
When buffer is dripping from the tubing connected to the bottom of the
column, connect this piece of tubing to the UV monitor.
ÄKTA go Operating Instructions 29360951 AC