Biotage VacMaster 20 Operation Manual

Sample processing station

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User manual (16 pages)

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Biotage

VacMaster

10 & 20

™

Sample Processing Station

Operation Manual

Table of Contents

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Summary of Contents for Biotage VacMaster 20

Page 2: Table Of Contents
©2013 Biotage Trademark Acknowledgement Biotage, Biotage ZIP, Advancer, Advancer Kilobatch, Advancer 350, AFFINILUTE, Endeavor, EVOLUTE, EVOLUTE EXPRESS, ExploraSep, Firefly design, FLASH+, FlashMaster, FlashVac, Horizon, HPFC, HP-SIL, HP-Sphere KP-Sphere, ZIP-Sphere, Initiator, Initiator+, Initiator+ Alstra, Initiator+ Robot 8, Initiator+ Robot 60, Initiator+ SP Wave, Isolera, Isolera Prime, Isolera One, Isolera Four,...
Page 3: Introduction
Introduction About the Biotage VacMaster ® ™ » A package of 10 or 20 stainless steel needles with port sealing plugs The Biotage VacMaster is a state-of-the-art sample processing ® ™ station. It incorporates innovative design features that make Optional accessories simultaneously processing multiple samples easier than ever »...
Page 4: Principle Of Operation
The maximum tube diameter possible with the VacMaster-10 is glass tank can be oriented two ways. Turn the fitting up to close 27 mm (accommodates scintillation vials), and VacMaster 20 is the outlet and add the appropriate sterilization solution to the 23 mm (order specially).
Page 5 Figure 1. Lid A. Vacuum gauge (p/n 0018) mounted on controller arm assembly (p/n 121-0025) B. Fine control valve (p/n 121-0020) C. Coarse vacuum control valve (Open or Closed) D. PTFE lid (for replacement lit complete with all fittings, order p/n 121- 1045 (VacMaster-10) or 121-2075 (VacMaster-20) E.
Page 6: Installation
Place a trap in the vacuum line In addition to the VacMaster and the accessories, you will need to collect any liquid for proper disposal. the following to use the VacMaster. © Biotage 2013 Biotage VacMaster Manual ®...
Page 7 Select collection tubes suitable for your rack and your intended use. Racks are available to accommodate 10, 12, or 16 mm tubes 4. Each plate is held in place by nut washers positioned above (and 27mm for Biotage VacMaster -10). If using a different and below the plate.
Page 8: Routine Operation
2. Open the stopcocks if they are being used. Open both the or other hazardous samples, it is advantageous to purchase a coarse and fine vacuum control valves on the Biotage second low cost tank to dedicate to the analyte elution step.
Page 9: Solid Phase Extraction
The most common » Purification method is vacuum applied to the column outlet. The Biotage ® » VacMaster vacuum manifold is specifically designed for this ™...
Page 10 SPE column). 0.5–1.0ml/100 mg sorbent. Alternatively, extract solids with a liquid prior to SPE. Recovery (%) Flow Rate Figure 4. Sample loading flow rate vs. extraction efficiency © Biotage 2013 Biotage VacMaster Manual ® ™...
Page 11 SPE Column Equilibration are often very good choices for wash solvents. If the wash Following solvation of the column, and prior to applying the solvent is immiscible with the solvent in which the sample was sample, it is necessary to equilibrate the column. Equilibrate a applied, a drying step is necessary.
Page 12: Maintenance And Troubleshooting
Warranty seriously compromise the structural integrity of the product. Biotage products are warranted to be free from defects in Consequently, Biotage can accept no liability for injury or materials and workmanship for a period of ninety (90) days damage caused by the VacMaster or its accessories.
Page 13: Ordering Information
Components for VacMaster-20 Needles and stopcocks for VacMaster-10 and -20 121-0044 VacMaster 20 rack handle 121-0001 PTFE stopcock / needle unit 121-2059 VacMaster 20 silicone lid gasket 121-0002 PTFE needle unit 121-2068 Spare glass tank with waste outlet 121-0003 Stainless steel needles...
Page 16 Your Complete Partner for Effective Chemistry Biotage is a worldwide supplier of instruments and accessories designed to facilitate the work of laboratory chemists. With our deep knowledge of the industry, academic contacts and in-house R&D teams, we can deliver the best solutions to your challenges.

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Vacmaster 10