Other Error Indications; Potential Problems - Causes And Remedies - Leica CM3050S Instructions For Use Manual

Cryostat
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6
Troubleshooting, application tips
Note
This message is displayed after a certain number of operating hours to remind the user to have a
preventive maintenance done by Leica Technical Service.
This message is displayed only, if the instrument is not regularly serviced by Leica Technical Service.
We recommend a maintenance contract once the warranty period has expired! Contact your local
Leica sales office for details!
If one of the error messages listed under
indications)
and

6.1.1 Other error indications

(red) in control panel 2 is illuminated:
» Emergency stop button accidentally activated. Solution: Unlock.
» Footswitch not properly connected. Solution: Check connection - connect properly, if applicable.
6.2

Potential problems - causes and remedies

Problem
Frost on chamber walls and
microtome
Sections smear.
Sections splinter
Sections not properly flattened.
64
(→ p. 63 – 4. Service)
is displayed, please contact Leica Technical Service.
Causes
• Cryostat is exposed to draft
(open windows, doors,
air-conditioning).
• Sliding window has been
open for a long period of
time at a very low chamber
temperature.
• Frost build-up caused
by breathing into the
cryochamber.
• Specimen not cold enough.
• Knife and/or anti-roll plate not
yet cold enough - sections
melt.
• Specimen too cold.
• Static electricity/draft.
• Specimen not cold enough.
• Large surface specimen.
• Anti-roll plate poorly adjusted. • Readjust anti-roll plate.
• Anti-roll plate poorly aligned
relative to knife edge.
• Wrong clearance angle
selected.
• Knife blunt or damaged.
(→ p. 63 – 2. Coarse
feed),
(→ p. 63 – 3. Temperature
Remedies
• Eliminate draft sources or
change place of installation of
the cryostat.
• Close sliding window.
• Consider wearing a mask.
• Select lower temperature.
• plate have reached chamber
temperature.
• Select higher temperature.
• Eliminate cause.
• Select lower temperature.
• Trim specimen parallel;
increase section thickness.
• Align correctly.
• Set correct clearance angle.
• Use different part of knife or
replace knife.
Version 2.0, Revision G

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