6
Troubleshooting, application tips
Note
This message is displayed after a certain number of operating hours to remind the user to have a
preventive maintenance done by Leica Technical Service.
This message is displayed only, if the instrument is not regularly serviced by Leica Technical Service.
We recommend a maintenance contract once the warranty period has expired! Contact your local
Leica sales office for details!
If one of the error messages listed under
indications)
and
6.1.1 Other error indications
•
(red) in control panel 2 is illuminated:
» Emergency stop button accidentally activated. Solution: Unlock.
» Footswitch not properly connected. Solution: Check connection - connect properly, if applicable.
6.2
Potential problems - causes and remedies
Problem
Frost on chamber walls and
microtome
Sections smear.
Sections splinter
Sections not properly flattened.
64
(→ p. 63 – 4. Service)
is displayed, please contact Leica Technical Service.
Causes
• Cryostat is exposed to draft
(open windows, doors,
air-conditioning).
• Sliding window has been
open for a long period of
time at a very low chamber
temperature.
• Frost build-up caused
by breathing into the
cryochamber.
• Specimen not cold enough.
• Knife and/or anti-roll plate not
yet cold enough - sections
melt.
• Specimen too cold.
• Static electricity/draft.
• Specimen not cold enough.
• Large surface specimen.
• Anti-roll plate poorly adjusted. • Readjust anti-roll plate.
• Anti-roll plate poorly aligned
relative to knife edge.
• Wrong clearance angle
selected.
• Knife blunt or damaged.
(→ p. 63 – 2. Coarse
feed),
(→ p. 63 – 3. Temperature
Remedies
• Eliminate draft sources or
change place of installation of
the cryostat.
• Close sliding window.
• Consider wearing a mask.
• Select lower temperature.
• plate have reached chamber
temperature.
• Select higher temperature.
• Eliminate cause.
• Select lower temperature.
• Trim specimen parallel;
increase section thickness.
• Align correctly.
• Set correct clearance angle.
• Use different part of knife or
replace knife.
Version 2.0, Revision G
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