Leica CM3050S Instructions For Use Manual
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Leica CM3050S Instructions For Use Manual

Cryostat
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See also: Instruction Manual
Leica CM3050S
Cryostat
Instructions for Use
English
Order No.: 14044380101 - Revision G
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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Summary of Contents for Leica CM3050S

  • Page 1 Leica CM3050S Cryostat Instructions for Use English Order No.: 14044380101 - Revision G Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 This document is protected under copyright laws. All copyrights to this documentation are held by Leica This document is protected under copyright laws. All copyrights to this documentation are held by Leica Biosystems Nussloch GmbH.

  • Page 4: Table Of Contents

    Table of Contents Important information ..........................Symbols and their meanings.......................... 7 General ................................9 Instrument type .............................. 10 Designated use .............................. 11 Qualification of personnel ..........................11 Safety ................................Safety notes ..............................12 Integrated safety devices..........................13 2.2.1 Locking the handwheel..........................13 2.2.2 Centering the handwheel grip ........................
  • Page 5 Disinfection at room temperature ....................... 70 Cleaning ................................70 Removing/reinstalling the microtome ......................71 7.5.1 Removing the microtome..........................71 7.5.2 Disassembling the specimen head (instruments w/specimen cooling only) ........71 7.5.3 Reinstalling the microtome .......................... 73 Exchanging the fluorescent light lamp....................... 74 Leica CM3050S...
  • Page 6 Table of Contents Warranty and service ..........................Appendix ..............................Electrohydraulic height adjustment......................77 9.1.1 Designated use .............................. 77 9.1.2 Standard delivery ............................77 9.1.3 Safety instruction ............................77 9.1.4 Installation ..............................78 9.1.5 Control keys ..............................79 9.1.6 Maintenance and care ..........................80 9.1.7 Troubleshooting .............................

  • Page 7: Important Information

    Symbols and their meanings Caution Leica Biosystems GmbH assumes no liability for consequential loss or damage due to failure to observe the following instructions, particularly in relation to transportation and package handling, and failure to observe the instructions for handling the instrument carefully.
  • Page 8 Important information Symbol: Title of the symbol: CE Compliance The CE marking is the manufacturer's declaration Description: that the medical product meets the requirements of the applicable EC directives. In vitro diagnostic medical device Symbol: Title of the symbol: Description: Indicates a medical device that is intended to be used as an in vitro diagnostic medical device.

  • Page 9: General

    Note Upon delivery of the instrument, the recipient must check that the tilt indicator is intact before unpacking. The responsible Leica representative must be notified in the case that the indicator has been triggered. General This Instructions for Use includes important instructions and information related to the operating safety and maintenance of the instrument.

  • Page 10: Instrument Type

    (→ p. 12 – 2. Safety). Please read this information, even if you are already familiar with the operation and use of other Leica products. Instrument type All information given in this instruction manual applies only to the instrument type indicated on the title page.

  • Page 11: Designated Use

    Important information Designated use • The Leica CM3050S is a powerful cryostat for routine as well as research applications in biology, medicine and industry. • The instrument has been designed for rapid freezing and sectioning of tissue samples. • The instrument has not been designed for unattended storage of tissue material.

  • Page 12: Safety

    • The safety and caution notes in this chapter must be observed at all times. Be sure to read these notes even if you are already familiar with the operation and use of other Leica products. • When working with cryostats, personal safety precautions must always be taken. It is mandatory to wear work safety shoes, safety and cut resistant gloves (→...

  • Page 13: Integrated Safety Devices

    (→ "Fig. 2-2"). 2. To lock, press pin (→ "Fig. 2-4") to the right into position (→ "Fig. 2-5"). L The upper locking position for pin (→ "Fig. 2-4") is marked by a black dot (→ "Fig. 2-7"). Leica CM3050S...

  • Page 14: Centering The Handwheel Grip

    Safety Instruments with sectioning motor: The sectioning motor is now blocked. All instruments: The message in the display of control panel 1 indicates that the handwheel has been locked: LOCKED ° ° Unlocking: 1. To unlock, push locking pin (→ "Fig. 2-4") to the left into position (→...

  • Page 15: Knife Guard

    • right: 30 cm - 11.8 inch • left: 15 cm - 5.9 inch The compressor oil may have been displaced during transport and must settle to its original position before switching the instrument on. Otherwise, the instrument may be severely damaged. Leica CM3050S...

  • Page 16: Site Requirements

    Safety 2.3.2 Site requirements Warning Do not operate in rooms with explosion hazard! To ensure proper function of the instrument, it must be set up with a minimum distance on all sides from walls and furniture (→ p. 15 – 2.3.1 Transport installation).

  • Page 17: Electrical Connections

    • Never leave any knives / blades in unprotected places. • Never place a knife, no matter where, with the cutting edge facing upwards. • Never try to catch a falling knife. • Always insert the specimen before inserting the knife. Leica CM3050S...

  • Page 18: Knife Guard/Handwheel Lock

    Safety 2.3.5 Knife guard/handwheel lock Warning Always cover the cutting edge with the knife guard and lock the handwheel: • Wear cut resistant gloves (→ p. 23 – 3.4 Standard delivery). • Prior to doing any work on knife and/or specimen. • Prior to exchanging specimens.

  • Page 19: Infectious/Radioactive Material

    • Place handwheel grip in lowest position and lock. When removing the microtome, the specimen head must always be locked in the lowest position. Otherwise the upper part of the slot cover might be bent and consequently damaged! Leica CM3050S...

  • Page 20: Display Message Dry Microtome

    2. Unplug the instrument from mains. Warning Risk of injury! If the lamp is broken or splintered, have lamp replaced by Leica Technical Service. 3. Use only those replacement lamps that correspond to technical specification (→ p. 30 – 4.2 Technical data).

  • Page 21: Installation

    Transport to the site: 1. First, check if the location meets the conditions specified in (→ p. 16 – 2.3.2 Site requirements) (→ p. 21 – 3.1.1 General site requirements) 2. Transport the instrument to the desired location. Leica CM3050S...
  • Page 22 Installation Warning Observe the following: • The instrument must be transported in an upright position or slightly tilted (max. 30 °). • When tilting the instrument 2 people must counterbalance from the front side to prevent the instrument from falling down and causing severe injury, as well as possible severe damage to the instrument and the surrounding area! 3.

  • Page 23: Repacking

    Installation 3.2.1 Repacking We recommend to keep the original shipping crate and the unpacking instructions for the Leica CM3050S. For repacking, proceed as per unpacking instructions, in reverse order. Available models • Basic instrument: with sectioning motor/without specimen cooling • Basic instrument: without sectioning motor/with specimen cooling • Basic instrument: with sectioning motor/with specimen cooling...
  • Page 24 • In case of non-configured instruments, the knife holder is not a part of standard delivery but must be ordered separately. • Check all delivered parts against the packing list and against your order to verify whether the delivery is complete. If there is any difference, contact your local Leica office immediately. Version 2.0, Revision G...

  • Page 25: Installing/Inserting Accessories

    8. Place knife case with knife into chamber to precool. 9. Place all tools needed for section preparation into the chamber. 10. Close the sliding window. Note For a complete overview of all individual parts, see (→ p. 28 – 4.1 Overview). Leica CM3050S...

  • Page 26: The Footswitch

    Installation 3.5.3 The footswitch Fig. 6 Function The footswitch (→ "Fig. 6") performs the same task as the keys RUN/STOP RUN/ENABLE (activating/deactivating motorized sectioning/trimming). In addition, the footswitch can be used to activate the emergency stop function. Models with footswitch: All instruments with sectioning motor.

  • Page 27: Prior To Switching On The Instrument

    • Observe all safety instructions in chapters (→ p. 16 – 2.3.2 Site requirements) (→ p. 17 – 2.3.3 Electrical connections). 1. Insert mains plug into wall outlet. 2. Continue with (→ p. 33 – 5.1 Operating the instrument for the first time). Leica CM3050S...

  • Page 28: Instrument Properties

    Instrument properties Instrument properties Overview Fig. 8 Version 2.0, Revision G...
  • Page 29 Instrument properties Fig. 9 Cryostat Leica CM3050S Thermoblock (optional) Control panel 1 Section waste tray Control panel 2 Brush shelf Storage shelf, left Specimen head w/o specimen cooling Storage shelf, right Specimen head with specimen cooling (Option) Rubber mat 90 ° Prism (instruments with specimen cooling only)

  • Page 30: Technical Data

    Instrument properties Technical data Type CM3050S CM3050S CM3050S Nominal voltage 100 V AC 230 V AC 240 V AC ±10 % ±10 % ±10 % Nominal frequency 50 Hz 50 Hz 50 Hz Power consumption 1800 VA 1800 VA 1800 VA Max.
  • Page 31 Number of freezing stations according to CECOMAF liquid temperature 45 °C, evaporation temperature -25 °C Warning *) Refrigerant and compressor oil must be replaced by service personnel qualified and authorized through Leica only. Microtome Rotary microtome Sectioning thickness setting 0.5 - 300 µm...
  • Page 32 Instrument properties Cryocabinet Dimensions Width (excluding handwheel) 800 mm Width (including handwheel) 882 mm Depth 766 mm Height (armrest) 840 mm Overall height 1040 mm Weight (incl. microtome, without specimen cooling) approx. 180 kg Storage conditions Temperature: 5 - 50 °C Humidity: <80%, non-condensing Transport temperature:...

  • Page 33: Operation

    If you find ‘Call Technical Service’ in the ‘Remedy’ column, please do so. Never try to solve these problems yourself. Switching on/fuses Switching on » To switch on the instrument, place switch (→ "Fig. 10-1") in upper lock-in position. Leica CM3050S...

  • Page 34: Control Panel 1

    Operation Fig. 10 Mains switch/automatic cutout (→ "Fig. 10-1") for the instrument: In addition to its function as switch, switch (→ "Fig. 10-1") is also the automatic cutout for the ON/OFF instrument. If the automatic cutout has been triggered (e.g. caused by overcurrent): »...

  • Page 35: Key Functions In Control Panel 1

    A series of standard indications is displayed, during which two basic parameters can be selected (with arrow keys (→ "Fig. 11-4")): • Language (of display indications) and speed level of sectioning motor, see (→ p. 55 – 5.5.3 Optional accessories for freezing and for removing of frozen specimens). Leica CM3050S...

  • Page 36: Display Indications When Switching On

    Operation 2. Menu function (activated via menu (→ "Fig. 11-2")): • Via the menu key a number of parameters can be selected, then be activated/deactivated via the lock/unlock (→ "Fig. 11-5") and/or changed via the arrow keys (→ "Fig. 11-4"). See (→...
  • Page 37 ((→ p. 69 – 7.1 Safety instructions on disinfection and cleaning) (→ p. 71 – 7.5 Removing/reinstalling the microtome)). *) Indication in instruments with specimen cooling. Instruments without specimen cooling: only (centered) is displayed. Leica CM3050S...

  • Page 38: Menu Functions: Setting Parameters Of Refrigeration, Time And Preset Counter

    Operation 5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter L Once the instrument has gone through the initial sequence of indications, you can go on to the menu functions to select and/or change instrument parameters. If display is locked: Press key until display background lights up. »...
  • Page 39 • In order to be able to set a reference value, the preset counter function must be active. • If the preset counter is deactivated (see diagrams above), press to activate. Now there are two options: 1. Increase or decrease the actual reference value by pressing Leica CM3050S...
  • Page 40 Operation 2. Press simultaneously in order to reload the former reference value when setting up the instrument) and then press either individually in order to select the desired reference value. Depending on where you start from and where you want to go, method 1 or method 2 is quicker (see examples).
  • Page 41 8-minute cycle. In such cases, increase the defrost cycle duration gradually until the the evaporator is ice-free after defrosting. The selected defrost cycle duration is valid for both automatic and manual chamber defrost cycle. Leica CM3050S...

  • Page 42: Status Display

    Operation 5.3.5 Status display Each individual parameter is displayed in a certain area of the display screen. (→ "Fig. 12-1"): Upper line In the menu (→ p. 38 – 5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter): indication of selected menu item. Standard indication: Chamber temperature (instruments with specimen cooling: chamber and specimen temperature).
  • Page 43 • Refrigeration OFF • Refrigeration ON  • Defrosting active  • Section thickness (set value) • Section counter (actual value) • Indication of total section thickness (actual value) • Preset counter Scroll via arrow keys to display individual parameters Leica CM3050S...

  • Page 44: Indication Of Refrigeration State, Section Thickness Setting And Counters

    Operation 5.3.6 Indication of refrigeration state, section thickness setting and counters These parameters can be displayed via the arrow keys. When pressing , the parameters are displayed in the sequence listed on this page; when pressing , they are displayed in reverse order. The display can be scrolled continuously, i.e. after the last parameter the display will automatically start again with the first.
  • Page 45 L Reloading the former reference value is possible also while the continuous sectioning mode is in motion:  Upon pressing both arrow keys, the instrument continues sectioning without interruption, starting to count down from the old reference value. Leica CM3050S...

  • Page 46: Control Panel 2

    Operation Control panel 2 Note The layout of the control panel 2 is different between the instruments with sectioning motor (→ "Fig. 14-1") and those without sectioning motor (→ "Fig. 14-2"). Fig. 14 Instruments with sectioning motor In instruments with sectioning motor, the control panel is equipped with three functional areas: (→...

  • Page 47: Selecting A Sectioning Mode/Start And Stop Sectioning

    The specimen head carries out one sectioning stroke and then stops in the lower limit position. Activating a sectioning mode » Press one of the three sectioning mode keys described above. While a sectioning mode is active, in the corresponding key will be illuminated. Leica CM3050S...

  • Page 48: Setting The Sectioning Window

    Operation Start/stop motorized sectioning Sectioning mode 1 - Intermittent stroke: 1. Simultaneously press and hold or depress and hold footswitch. RUN/STOP RUN/ENABLE 2. To stop the sectioning stroke, release the keys or the footswitch: The specimen head stops immediately. Sectioning mode 2 - Continuous stroke: 1.

  • Page 49: Selecting Sectioning Speed

    • If the slider is accidentally moved, sectioning will be resumed - Risk of injury! Therefore: • Do not interrupt sectioning/trimming by setting the sliding potentiometer to zero speed! • See also safety instruction in (→ p. 18 – 2.3.6 Motorized sectioning). Leica CM3050S...

  • Page 50: Emergency Stop

    Operation Vmax As long as the key is pressed, the sectioning motion is carried out at maximum speed (210 mm/s). Note While is active, the sectioning window is inactive. Vmax When the Vmax key is released, the sectioning window becomes active again. 5.4.4 Emergency stop Note The following desriptions are only valid for the functional areas...

  • Page 51: Coarse Feed Keys

    As long as the forward coarse feed (both fast and slow) is in motion, the stop LED illuminated. Upon reaching the front limit position, the starts flashing and the display in stop LED control panel 1 reads STOP. Leica CM3050S...

  • Page 52: Trimming And Sectioning Functions - Switching Between Trimming And Sectioning

    Operation 5.4.6 Trimming and sectioning functions - switching between trimming and sectioning Note The following descriptions are valid for all models. See functional area (→ "Fig. 14-4"). Press this key to alternate between the two functions. Illuminated: Trimming mode is active. Off: Sectioning mode is active.

  • Page 53: Daily Operation Of The Instrument

    2. Apply sufficient amount of embedding medium onto ambient temperature specimen disc. 3. Inside the cryochamber, place the specimen onto the disc and orient. 4. Place specimen disc with specimen into one of the 10 openings in the quick-freeze shelf (→ "Fig. 15") and freeze. Leica CM3050S...

  • Page 54: Freezing Specimens Onto Specimen Discs With 90 ° Prism (Instruments W/Specimen Cooling)

    Operation Fig. 15 L The freezing process can be sped up by using the stationary heat extractor (→ "Fig. 15-1"). Next to the low temperature stabilizer (→ "Fig. 15-2") (Parking station), 3 specimen discs (→ "Fig. 15-3") can be placed within its pivoting range. »...

  • Page 55: Optional Accessories For Freezing And For Removing Of Frozen Specimens

    Mobile heat extractor In addition to the stationary heat extractor (→ p. 23 – 3.4 Standard delivery), a mobile heat extractor (→ "Fig. 17") is available as an optional accessory (→ p. 81 – 9.2 Ordering information - accessories): Leica CM3050S...
  • Page 56 Operation Fig. 17 1. Store heat extractor in chamber to precool. 2. Place onto specimen in quick-freeze shelf. 3. Remove as soon as specimen is frozen. Thermoblock The thermoblock makes it easy to remove frozen specimens from the specimen discs. Note Do not store the thermoblock inside the cryochamber - store at room temperature and only place into the chamber for removing specimens from discs.

  • Page 57: Inserting Specimen Discs Into The Specimen Head

    For details on how to insert and adjust the knives, please refer to the separate instruction manual(s) for your knife holder(s). See also chapter (→ p. 13 – 2.2 Integrated safety devices) (→ p. 15 – 2.2.4 Knife guard). Leica CM3050S...
  • Page 58 Operation Manual trimming 1. Adjust knife / knife holder relative to specimen (see ‘Knife holder’ Instructions for Use). 2. Unlock handwheel. 3. Use motorized coarse feed to bring specimen close to knife (→ p. 51 – 5.4.5 Coarse feed keys). 4.

  • Page 59: Finishing Work

    If the instrument is switched off, the microtome must be removed. Then the microtome, cryochamber and all accessories must be disinfected and dried. • For that purpose, follow the instructions in (→ p. 69 – 7.1 Safety instructions on disinfection and cleaning) through (→ p. 73 – 7.5.3 Reinstalling the microtome). Leica CM3050S...

  • Page 60: Defrosting

    Operation Defrosting The Leica CM3050S has 3 (instruments without specimen cooling: 2) different defrosting options. • Automatic chamber defrost cycle • Manual chamber defrost cycle • Specimen head defrosting (manual) - instruments with specimen cooling only. Warning Read the safety instructions (→...

  • Page 61: Manual Chamber Defrost Cycle

    - see (→ p. 60 – 5.7.1 Automatic chamber defrost cycle)). 10 seconds after the manual chamber defrost cycle has been completed, the specimen head refrigeration is switched back on, cooling down to the set value. Leica CM3050S...

  • Page 62: Defrosting The Specimen Head (Instruments With Specimen Cooling Only)

    Operation 5.7.3 Defrosting the specimen head (instruments with specimen cooling only) To activate specimen head defrosting: Press key until menu item is displayed: Set specimen head temperature E M P °   Press key. A brief sound signals that the manual specimen head defrost cycle has started: E M P °...

  • Page 63: Troubleshooting, Application Tips

    Troubleshooting, application tips Troubleshooting, application tips Displayed error messages The autodiagnosis function of the Leica CM3050S displays error messages in case of certain malfunctions. 1. Power failure • This error message is displayed after the instrument had been disconnected from mains, either due to power failure or because the mains switch had been turned off.

  • Page 64: Other Error Indications

    This message is displayed after a certain number of operating hours to remind the user to have a preventive maintenance done by Leica Technical Service. This message is displayed only, if the instrument is not regularly serviced by Leica Technical Service. We recommend a maintenance contract once the warranty period has expired! Contact your local...
  • Page 65 Condensation forms on anti-roll • Brush, forceps, cloth or other • Accessories and cleaning plate and knife during cleaning. cleaning item too warm. material to be used pre-cooled only. • Store on storage shelves inside the cryochamber. Leica CM3050S...
  • Page 66 Troubleshooting, application tips Problem Causes Remedies Anti-roll plate damaged after • Anti-roll plate protrudes too • Rotate or replace anti-roll adjustment. far beyond the knife edge. plate; in future, lift away from Adjustment was done in the the knife when aligning. direction of the knife.
  • Page 67 • Call Technical Service. No or insufficient refrigerating • Stopper not placed properly • Close drain with stopper. performance. into drain in bottom of cryo-chamber. • Compressor defective. • Call Technical Service. • Leak in cooling system. • Call Technical Service. Leica CM3050S...

  • Page 68: Reference Chart For Temperature Settings (In Minus °C)

    Troubleshooting, application tips Reference chart for temperature settings (in minus °C) Tissue 10-15 15-25 25-50 Adrenal   Bone marrow  Brain    Bladder Breast - fatty  Breast - little fat  Cartilage    Cervical Fatty ...

  • Page 69: Disinfection, Cleaning And Maintenance

    Note Wait until the spray mist has settled down on the surfaces and is no longer visible and turn the instrument back on. The maximum waiting period should not extend 10 minutes, to avoid de-icing of the chamber. Leica CM3050S...

  • Page 70: Disinfection At Room Temperature

    Disinfection, cleaning and maintenance 9. Use paper towels or another type of disposable cleaning cloths to wipe up disinfectant and remaining section waste. 10. Dispose of paper towels together with section waste. 11. If previously altered, set chamber temperature back to its former value. 12.

  • Page 71: Removing/Reinstalling The Microtome

    (→ "Fig. 21-3") (→ p. 23 – 3.4 Standard delivery) in one of your hands and with your other hand rest refrigerating tube (→ "Fig. 21-2") on hook. 4. Insert hook (→ "Fig. 21-3") with refrigerating tube (→ "Fig. 21-2") into the opening above mark (). Leica CM3050S...
  • Page 72 Disinfection, cleaning and maintenance Fig. 21 5. Remove plug of temperature sensor (→ "Fig. 22-5"). Remove plug of coarse feed motor: 1. Pull bushing backward (→ "Fig. 22-1") and hold. 2. Place your thumb between plug (→ "Fig. 22-4") and socket at the microtome (→...

  • Page 73: Reinstalling The Microtome

    (→ "Fig. 22-5"). 11. Mount specimen head (in reverse order as described on previous page). 12. Insert accessories (storage shelf, section waste tray etc.). 13. Insert sliding window. 14. Connect instrument to mains and switch back on. Leica CM3050S...

  • Page 74: Exchanging The Fluorescent Light Lamp

    Disinfection, cleaning and maintenance Exchanging the fluorescent light lamp Warning Prior to exchanging the lamp, please read and observe the following safety instructions: (→ p. 20 – 2.3.13 Maintenance) Removing the sliding window » Hold sliding window (→ "Fig. 24-1") at grip bar (→...
  • Page 75 2. Push the lamp slightly upwards until it locks into the clip. Fig. 26 3. Put the sliding window back in place. 4. Reconnect the instrument to mains and switch it back on. Leica CM3050S...

  • Page 76: Warranty And Service

    The scope of the warranty is based on the content of the concluded agreement. The warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively. Technical service information If you require technical service or replacement parts, please contact your Leica sales representative or dealer who sold the product.

  • Page 77: Appendix

    The electrohydraulic height adjustment device has been designed exclusively for adjustment of the instrument working level to the individual height of the user. It has been designed for use with the Leica cryostat series Leica CM3050S only and may under no circumstances be used to lift other instruments or persons.

  • Page 78: Installation

    Do not move the height adjustment device with the Leica CM3050S installed on the frame. If the place of installation needs to be changed, first take the Leica CM3050S down from the height adjustment device and then move both devices separately.

  • Page 79: Control Keys

    • To lift the instrument, press (→ "Fig. 28-1"). • To lower the instrument, press (→ "Fig. 28-2"). As long as a button is held, the instrument will go up or down until reaching the upper or lower limit. Leica CM3050S...

  • Page 80: Maintenance And Care

    • Protect the lifting pistons in the four legs of the height adjustment device against dust and water. • Approximately every six months lubricate the legs with a thin coat of resin-free oil. • Once every year have the height adjustment device checked by an authorized Leica service engineer. 9.1.7 Troubleshooting • Slight and uniform loss of height of the device in the course of several weeks is normal.

  • Page 81: Technical Data

    • The height adjustment device does not reach the maximum upper level or the instrument lowers itself by a relatively large distance (> 5 cm) within a short time. Remedy: L Call Leica Technical Service. 9.1.8 Technical data Nominal voltage:...
  • Page 82 14 0443 25949 Anti-static kit low-prof. 14 0800 37739 Anti-static kit high-prof. 14 0800 37740 Height adjustment module Leica CM3050S, 100-115 V/100-120 V/50 Hz 14 0443 26148 Height adjustment module Leica CM3050S, 230/240-250 V/50-60 Hz 14 0443 26147 Dr.Peters Cryoembedding systems...

  • Page 83: Decontamination Confirmation

    Decontamination confirmation Decontamination confirmation Every product that is returned to Leica Biosystems or that requires on-site maintenance must be properly cleaned and decontaminated. You can find the dedicated template of the decontamination confirmation on our website www.LeicaBiosystems.com within the product menu. This template has to be used for gathering all required data.
  • Page 84 Note Version 2.0, Revision G...
  • Page 86 Leica Biosystems Nussloch GmbH Heidelberger Str. 17 - 19 69226 Nussloch Germany Tel.: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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