Thermo Scientific NanoDrop 2000 Instruction Manual

Quick Start
1. Double click on the desktop NanoDrop™ 2000 software icon and select
the application of interest. Follow the prompts for instrument initializa-
tion.
2. Ensure Add to report is selected in the left pane to automatically in-
clude all measurements in the saved report.
3. Establish a Blank using the appropriate buffer. Pipette 1-2 ul of the
blanking buffer onto the bottom pedestal, lower the arm and click the
Blank button. The blank solution is generally the same buffer that the
molecule of interest is suspended or dissolved in.
−
For the NanoDrop 2000c model, select the Use cuvette box to make
measurements with a cuvette.
−
Insert the cuvette noting the direction of the light path indicated by
the etched arrow.
−
The arm must be down for all measurements-including measure-
ments made with cuvettes.
−
The optical path is directed 8.5 mm above the bottom of the cu-
vette. Refer to the manufacturer for volume recommendations.
4. Wipe away the blank and enter the sample ID in the appropriate field.
Pipette 1-2 ul of sample and hit Measure.
−
It is recommended that a fresh aliquot of sample be used for each measurement.
After a measurement:
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Wipe both measurement pedestals using a dry, lint-free laboratory wipe and the instrument is ready for the next sample.
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When using the cuvette option, remove the cuvette, rinse thoroughly and dry between samples.
Blanking Cycle
It is generally recommended that an aliquot of the blanking buffer be measured as if it were a sample. This will confirm that the in-
strument is working well and that any sample dried down from previous measurements is not a concern. To run a blanking cycle,
perform the following:
1. Load an aliquot of the blank onto the lower measurement pedestal and lower the sampling arm into the down position.
2. Click on the Blank button to store the blank reference.
3. Analyze a fresh replicate of the blank as though it were a sample by selecting Measure. The result should be a spectrum that
varies no more than 0.04 A (10 mm absorbance equivalent).
Wipe the blank from both measurement pedestal surfaces and repeat the process until the spectrum is
4.
path).
Although it is not necessary to blank between each sample, it is recommended that a new blank be taken every 30 minutes when
measuring many samples.
For Technical Support contact us at 302-479-7707 or send an email to nanodrop@thermofisher.com.
Thermo Fisher Scientific - NanoDrop products
I N S T R U C T I O N
Thermo Scientific NanoDrop 2000/2000c Spectrophotometers
Wilmington, Delaware USA
S
Pedestal measurement
302-479-7707
NanoDrop 2000/2000c
Cuvette measurement
within 0.04 A (10 mm
www.nanodrop.com