Sample Preparation High Sensitivity D5000 Screentape Assay - Agilent Technologies 2200 User Manual

4
Using the 2200 TapeStation System
DNA Sample Preparation

Sample Preparation High Sensitivity D5000 ScreenTape Assay

Prepare TapeStation System HSD5000
Parts required p/n
5067-5592
1 Launch the 2200 TapeStation Controller Software.
2 Load single High Sensitivity D5000 ScreenTape device and loading tips
Sample Preparation High Sensitivity D5000 ScreenTape Assay
Parts required p/n
5067-5593
1 Allow reagents to equilibrate at room temperature for 30 min
2 Vortex mix before use
3 Prepare ladder by mixing 2 µL High Sensitivity D5000 Sample Buffer
Use a fresh ladder for each run. No electronic ladder is available for the High Sensitivity
N O T E
D5000 assay.
4 Prepare sample by mixing 2 µL High Sensitivity D5000 Sample Buffer
5 Spin down, then vortex using IKA vortexer and adaptor at 2000 rpm
6 Spin down to position the sample at the bottom of the tube.
58
Description
High Sensitivity D5000 ScreenTape
into the 2200 TapeStation instrument
Description
High Sensitivity D5000 Reagents
( ) with 2 µL High Sensitivity D5000 Ladder ( ) in a tube strip.
( ) with 2 µL DNA sample in the same tube strip.
for 1 min.
Agilent 2200 TapeStation System - User Manual