Agilent Technologies 2200 TapeStation Quick Manual

The Agilent 2200 TapeStation system is an automated platform for simpler, faster and more reliable
electrophoresis. It is made up of 3 elements:
• 2200 TapeStation system (G2964AA) or 2200 TapeStation Nucleic Acid system (G2965AA)
• High Sensitivity D1000 ScreenTape (5067- 5584) with High Sensitivity D1000 Reagents (5067- 5585)
• Agilent Software packages (2200 TapeStation Controller Software, and TapeStation Analysis Software)
Kits
The High Sensitivity D1000 ScreenTape system is designed for analysing DNA molecules from 35 – 1000 bp.
Specifications
Analytical Specifications
Sizing Range
Typical Resolution
1
Sensitivity
Sizing Precision
2
Sizing Accuracy
Quantitative Precision
3
Quantitative Accuracy
Quantitative Range
Maximum sample buffer strength
Physical Specifications
Analysis Time
Samples per consumable
Sample volume required
Kit stability
Kit size
1
signal- to- noise >3 (single peak)
2
Sizing Accuracy for electronic ladder: ±20%
3
Measured against 2100 Bioanalyzer system
Agilent High Sensitivity D1000
ScreenTape System Quick Guide
High Sensitivity D1000 ScreenTape assay and reagents
35 – 1000 bp
35 – 300 bp: 15 %; 300 – 1000 bp: 10 %
5 pg/µL
5 % CV
±10 %
15 % CV
±20 %
10 – 1000 pg/µL
7 mM KCl, 20 mM Phosphate Buffer, 20 mM Guanidine-HCl, 80 mM NaCl, 20 mM Acetate
16 samples: <20 min; 96 samples: ≈100 min
16
2 µL
4 months
112 samples
Agilent Technologies