Leica ST4020 Instructions For Use Manual
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Leica ST4020 Instructions For Use Manual

Small linear stainer
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Instructions for Use
Leica ST4020
Small Linear Stainer
Leica ST4020, English
Order No.: 14 0509 80101 RevE
Always keep this manual with the instrument.
Read carefully before working with the instrument.

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Summary of Contents for Leica ST4020

  • Page 1 Instructions for Use Leica ST4020 Small Linear Stainer Leica ST4020, English Order No.: 14 0509 80101 RevE Always keep this manual with the instrument. Read carefully before working with the instrument.
  • Page 3 Leica reserves the right to change technical derstand it following thorough investigation in specifications as well as manufacturing pro- this field.

  • Page 4: Table Of Contents

    Table of Contents Important Information ....................... 6 Symbols in the text and their meanings ..................6 Safety ............................7 Safety notes ........................... 7 Warnings ............................7 Instrument Components and Specifications ............... 10 Overview - instrument components ..................10 Instrument specifications......................11 Functional description of components - system overview ..........
  • Page 5 Troubleshooting ........................40 General ............................40 Alarms ............................40 Fault detection and correction list ................... 41 Power failure ..........................43 Optional Accessories ......................44 Ordering information ........................44 Warranty and Service ......................50 Decontamination Declaration (master) ................51 Leica ST4020...

  • Page 6: Important Information

    Important Information Symbols in the text and their meanings Intended use The Leica Small Linear Stainer ST4020 is used Warnings for the automated preparation of routine histo- appear in a gray box and are marked logical and cytological stains. It is intended for...

  • Page 7: Safety

    Alternatively, they can be downloaded from the following website: http://www.msdsonline.com The protective devices located on the instrument and the accessories must not be removed or modified. Only service personnel qualified by Leica may repair the instrument and access the instrument's internal components. Warnings The safety devices installed in this instrument by the manufacturer only constitute the basis for accident prevention.
  • Page 8 The instrument will automatically detect the voltage/frequency of the power source. The installation location must be well ventilated, and must contain no sources of ignition of any kind. The chemicals used in the Leica ST4020 are easily inflammable and hazardous to health.
  • Page 9 Prevent liquids from entering the interior of the instrument while the instrument is being cleaned or during operation. Do not use any attachments or accessories not recommended by the product manufacturer as such attachments and accessories may cause hazards, damage the instrument and void the warranty. Leica ST4020...

  • Page 10: Instrument Components And Specifications

    Instrument Components and Specifications Overview - instrument components Adjustable Specimen side Rinsing Water Exit Tank Platform Foot carrier Container Reagent Lift Bar Hood Display Container (optional) Keypad Fig. 2 Instructions for Use V1.2, RevE – 08/2016...

  • Page 11: Instrument Specifications

    Foot Fig. 3 Instrument specifications The Leica ST4020 is an automatic linear stainer The user loads the slides in a carrier that can hold up to 4 slides at a time. The stainer provides designed for stand-alone use, and can be used...

  • Page 12: Functional Description Of Components - System Overview

    Figure page 10 shows an overview of the stainer. The Leica ST4020 Linear Stainer has the following functional areas: Keypad (14) with Display (13) A membrane keypad with 6 keys allows the user to set up the pro- cessing parameters and also allows the user to start and stop the processing runs.
  • Page 13 The stainer is connected to the power supply (12) by the power supply cord, and activated with the main switch (11). The instrument can be operated with alternating current at 100 to 230 Volt with a power frequency of 50/60 Hz. Fig. 11 Leica ST4020...

  • Page 14: Functional Areas Of The Slide Carrier

    Instrument Components and Specifications Functional Areas of the Slide carrier Handle Side Support Locating slots Side for slides Support Fig. 12 Fig. 13 show how the slide carriers are to be positioned over the reagent containers. While placing a slide carrier with slides for staining in the Lift Bar, ensure that the side supports of the slide carrier are aligned so that Slides the slide carrier is positioned above the container.

  • Page 15: Standard Delivery - Packing List

    Check the delivery carefully against the packing list, delivery note and your order. Should you find any discrepancies, please contact your Leica sales office without delay. Leica ST4020...

  • Page 16: Technical Data

    Instrument Components and Specifications Technical data General Admissions: UL, cUL, CE Nominal voltage: 100 V – 240 V +/- 10 % AC Nominal frequency: 50 to 60 Hz Power draw: 1 A max. IEC 1010 classification: Protection class 1 Pollution degree 2 Overvoltage category II Secondary fuses: Melting fuses 1.0 A T Ø...

  • Page 17: Instrument Setup

    • The chemicals used in the Leica ST4020 are easily inflammable and hazardous to health. • The installation site for the Leica ST4020 must be well ventilated, and there must be no ignition sources of any kind in the area.
  • Page 18 Instrument Setup Unpacking the Instrument (continued) These unpacking instructions only ap- ply if the box is placed with the sym- bols facing upwards for unpack- ing. 1. Cut through the packing tape (35) on the top edge of the box. Fig.

  • Page 19: Setup

    20), reinsert but do not fully tighten the two screws. • Press the support bracket down onto the sup- port surface until a face of the plate lies flush with the bench surface. Tighten the plate in this position. Fig. 20 Leica ST4020...

  • Page 20: Water Connection

    Instrument Setup Water Connection • Run the drain hose (9) into a suitable collecting tank or waste basin. Make sure that the hose is not kinked and that the water is able to drain freely downwards. • Then connect the rinsing water supply (35) to the stainer.
  • Page 21 Fig. 23 The hose (15) should run level along the bottom of the instru- ment. Mark the end of the hose that will connect to the rinsing wa- ter container. This is "Marking 1". Marking 1 Fig. 24 Leica ST4020...
  • Page 22 Instrument Setup Installing the rinsing water containers (continued) • Now take the rinsing water container and the hose out of the stainer. • Align marking 1 to the end of the connector and make a second marking ("Marking 2") Side surface of the connector to indicate the lateral surface of the connector (Fig.
  • Page 23 (18). Fig. 27 If two rinsing stations are to be located imme- diately beside one another, the connector (19) for the left station must be aligned as shown in Fig. Fig. 28 Leica ST4020...

  • Page 24: Remedy If Hose Is Too Short

    Instrument Setup Remedy if hose is too short If the hose (21) at one of the stations should be too short, or if a hose has been cut too short by mistake, the following remedy may help: Fig. 29 • Cut the hose roughly in the middle between the Y-connector and the rinsing station.

  • Page 25: Operation

    • Then, the Lift Bar performs a circular motion to ensure that the arm is in the correct position at the start of staining. The message FINDING HOME... is displayed on the screen. Fig. 31 Leica ST4020...
  • Page 26 Operation Control panel functions (continued) There are six pushbuttons on the control panel, and these are used to adjust the operating parameters and control the instrument. The keys have been assigned the following functions: The operating parameters can be displayed and checked with the MENU key.

  • Page 27: Setting The Operating Parameters

    30 to 60 seconds, ten seconds at a time in a range from 60 to 300 seconds. • After ENTER is pressed, the new processing time is activated, and this time is stored for future use. Leica ST4020...
  • Page 28 Operation Display Contrast The contrast on the liquid crystal display can be adjusted accord- ing to the preferences of individual users. • The adjustment range is between 1 and 15, 1 being the brightest setting. Transfer Mechanism Calibration The position at which the transfer movement is paused and pro- cessing started may be adjusted by pressing the PLUS or MINUS key ('+' or '–') followed by pressing the ENTER key.
  • Page 29 • With the "01" setting, the stainer runs continuously whether it is loaded or not. A beep sounds every time a slide carrier is ready. • Adjustment takes place using the PLUS or MINUS key. After ENTER is pressed, the selected start position is stored. Leica ST4020...

  • Page 30: Setting The Rinsing Water Flow Rate

    Operation Setting the rinsing water flow rate To set the flow rate for the rinsing water, proceed as follows: • First set a staining time of 60 seconds. To do this, press MENU once, and then press the PLUS key repeatedly until a process- ing time of 60 seconds is displayed. • Then press the ENTER key to confirm this processing time. • Now press RUN.

  • Page 31: Processing Slides

    Operation Processing slides The Leica ST4020 Stainer is a relatively simple and trouble-free system. The user must inform the system via the keypad whenever slides are to be added to or removed from the stainer. Directions as to how this is done appear in the following sections. Slides must be loaded and unloaded in the prescribed fashion.

  • Page 32: Starting A Staining Run

    Operation 5.5.1 Starting a staining run It is, of course, the user's responsibility to verify that each of the setup parameters (station time, number of steps, number of dips, etc.) is set to an acceptable value before slides are placed in the starting position and RUN is pressed.

  • Page 33: Adding More Slide Carriers During A Staining Run

    Therefore, the operator should be prepared to load the new slides as quickly as possible and should then press the RUN key quickly to avoid having all slides in the system spend extra time immersed. Leica ST4020...

  • Page 34: Temporarily Pausing A Staining Run

    Operation 5.5.3 Temporarily pausing a staining run If the PAUSE/STOP and RUN keys are pressed in this order, the instrument receives the information that more slide carriers have been inserted at the start position. The stainer continues operating until these slide carriers are transferred from station 14 to the exit tank.

  • Page 35: Ending A Staining Run Prematurely

    This is then the same situation as when the instrument is first switched on. • The corresponding message appears in the display. • As soon as the transfer mechanism reaches the start position, the stainer returns to idle mode; The same starting message is displayed after successful initialization that is displayed upon switching. Leica ST4020...

  • Page 36: Removing Processed Specimen Slides

    Operation 5.5.5 Removing processed specimen slides To be able to loosen and remove stained specimen slides from the specimen slide carrier easily and safely, grip the individual slides in sequence carefully with two fingers, move them back and forth slightly and pull them out. Usually, only a few movements are necessary.
  • Page 37 2. To remove the carriers with stained slides and load fresh slide carriers: • Press the PAUSE/STOP key once. • Wait until the system prompts you to load the slides and press the RUN key. • Place the new slide carriers in the system. • Press the RUN key once. • Remove ALL the slides from the exit tank. Leica ST4020...

  • Page 38: Cleaning And Maintenance

    Cleaning and Maintenance Cleaning the Instrument Prior to cleaning the instrument, always switch off power supply and unplug the power cord! When using cleaners, comply with the safety instructions from the manufacturer and the labor-safety regulations at your laboratory. Dispose of spent reagents in accordance with the laboratory guidelines in force in your country! Wipe off spilled solvents (reagents) immediately! The Hood surfaces only have mod- erate resistance in the event of prolonged contact with solvents!

  • Page 39: Maintenance Instructions

    +85 °C), because the heat can deform the stations! Maintenance Instructions Only authorized and qualified Leica service personnel may repair the instrument and access the instrument’s internal components. For your safety, never attempt to repair the instrument yourself.

  • Page 40: Troubleshooting

    If faults occur that cannot be corrected with the recommended procedures, or if they occur again, Leica Service Support must be informed immediately. General The Leica ST4020 is equipped with a simple fault detection system, and can identify certain movement errors automatically. • If an operation error is detected, the transfer mechanism will first stop moving.

  • Page 41: Fault Detection And Correction List

    • The motor does not turn. • Motor or control board de- • Inform Technical Service. fective. • Keypad does not respond to • The keypad or the control • Inform Technical Service. key presses board is defective. Leica ST4020...
  • Page 42 Troubleshooting Issue Possible cause Corrective action • Water does not flow. • Water hose is not connected. • Connect the water hose to the stainer and the tap. • Tap is not open. • Turn on the tap. • The valve in the instrument • Inform Technical Service. is faulty (does not open) or the control board is defec- tive. • Flow restrictor is clogged. • Replace flow restrictor.

  • Page 43: Power Failure

    If not, the fuse must be replaced. Before plugging the power cable back in and switching on the instrument, you must have identified and correct- ed the cause of the faulty fuse. • Insert the fuse insert with the two fuses and start up the instrument again. Fig. 33 Leica ST4020...

  • Page 44: Optional Accessories

    Optional Accessories Ordering information Order No. Reagent containers (6 in a pack) ....................14 0509 46437 Reagent containers (48 in a pack) ....................14 0509 46439 Slide Carriers (4 in a pack) ......................14 0509 46438 Slide Carriers (48 in a pack) ......................14 0509 46440 Wash station (1 container, 1 cleaning nozzle, 1 hose connector, 1 hose) ......
  • Page 45 4 in a pack Order-No. 14 0509 46438 48 in a pack Order-No. 14 0509 46440 Fig. 35 Wash Station Consisting of: 1 container, 1 cleaning nozzle, 1 hose connector, 1 Hose Order-No. 14 0509 46441 Fig. 36 Leica ST4020...
  • Page 46 Optional Accessories Rinse Tubing 61 cm long, Ø 0.32 cm Order-No. 14 0509 46457 Fig. 37 Tubing and Fitting Kit 1.22 m hose for wash station - Ø 0.32 cm, 2 Y-fittings, 3 in-line pipe unions, 2 plugs, 10 cable ties Order No.
  • Page 47 Adapter kit for Water Inlet Hose Connection to Faucets (including 1.9 cm NPT external thread) Order No. 14 0509 46444 Fig. 41 Drain hose 1.52 cm long, Ø 1.6 cm, 0.32 cm cable tie Order No. 14 0509 46445 Fig. 42 Leica ST4020...
  • Page 48 Optional Accessories Exit Tank Order No. 14 0509 46450 Fig. 43 Support Bracket Order No. 14 0509 46570 Fig. 44 Stabilizer Bar Order No. 14 0509 46451 Fig. 45 Reagent Container Cover, metal Order No. 14 0509 46442 Fig. 46 Instructions for Use V1.2, RevE –...
  • Page 49 Optional Plexiglas Hood Order No. 14 0509 46478 Fig. 47 Packaging, with foam, outer carton, bubble wrap, and accessory box) with packaging instructions Order-No. 14 0509 46455 Fig. 48 Outer Carton Only Order-No. 14 0509 46456 Fig. 49 Leica ST4020...

  • Page 50: Warranty And Service

    Leica Biosystems Nussloch GmbH guarantees that the contractual product delivered has been subjected to a comprehensive quality control proce- dure based on the Leica in-house testing standards, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted.

  • Page 51: Decontamination Declaration (Master)

    Dear Customer, Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems for repair.
  • Page 52 If no**, please indicate why not: Decontamination Declaration (master) Such equipment must not be returned without the written agreement of Leica Biosystems. The equipment has been prepared to ensure safe handling/transportation. Whenever possible, please use the original transportation case/box. Important - to avoid refusal of shipment: Place one copy in the unit prior to packaging, or hand it over to the service engineer.
  • Page 54 Leica Biosystems Nussloch GmbH Heidelberger Strasse 17-19 D- 69226 Nussloch Phone: +49 - (0) 6224 - 143 0 Fax: +49 - (0) 6224 - 143 268 Web: www.LeicaBiosystems.com...

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