Cellular analysis system, hematology specimen processing module with system manager (666 pages)
Summary of Contents for Beckman Coulter AU5800
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Quick Response Guide ® AU5800 Chemistry Analyzer B00418AA October 2010 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821...
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Beckman Coulter Biomedical Ireland Lismeehan O'Callaghans Mills Co Clare Ireland Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda Estr dos Romeiros, 220 - Galpao G3 - Km 38.5 06501-001 - Sao Paulo - SP - Brasil CNPJ: 42.160.812/0001-44 製造販売元...
Contents CHAPTER 1: Introduction, 1-1 1.1 Typographical Conventions Used in this Guide, 1-2 1.1.1 Tips, Cautions and Warnings, 1-2 1.1.2 Software Paths, Menus, and Tabs, 1-3 1.1.3 Software Buttons, 1-5 1.1.4 Images of Software Screens, 1-5 1.1.5 Analyzer Modes, 1-6 1.1.6 Notation of Units, 1-6 1.1.7...
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Contents CHAPTER 3: Daily Start Up, 3-1 3.1 Start the System, 3-2 3.1.1 Turn On the System, 3-2 3.1.2 Set the Start Conditions, 3-3 3.2 Perform Daily Maintenance, 3-6 3.3 Check Analyzer Status, 3-6 3.4 ISE (Optional Unit) Start Up, 3-8 3.4.1 Check the ISE Reagent, 3-8 3.4.2 Perform ISE Daily...
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Contents Computer, 4-23 4.7 Processing Emergency Samples, 4-26 4.7.1 Processing emergency samples in red racks from the priority rack set unit, 4-26 4.7.2 Processing emergency samples in red racks from the rack set unit, 4-26 4.7.3 Requisition for an Emergency Sample, 4-27 CHAPTER 5: Monitoring...
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Contents 6.7.1 Print Sample Data Reports, 6-23 6.7.2 Print Reagent Blank, Calibration, and QC Results, 6-25 6.8 Transfer Data to Host Computer, 6-28 6.8.1 Sample Data, 6-28 6.8.2 Reagent Blank, Calibration, and QC Data, 6-29 6.9 Fix (Assign) a Reagent Position, 6-30 6.10 Edit a Reagent ID, 6-32...
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Contents 7.5.1 Return to Standby Mode, 7-9 CHAPTER 8: Troubleshooting, 8-1 8.1 Using the Online Help, 8-2 8.2 Review Alarms and Corrective Actions, 8-3 8.3 Review Error Flags, 8-5 Index B00418AA...
This Quick Response Guide describes basic system operations. It contains general information on preparing for analysis, starting analysis and obtaining results. It also describes other frequently used procedures performed during daily routine operations. For detailed procedures, refer to the AU5800 User’s Guide. This chapter discusses the following.
Introduction 1.1 Typographical Conventions Used in this Guide 1.1 Typographical Conventions Used in this Guide This section describes the conventions used in the text of this guide: 1.1.1 Tips, Cautions and Warnings 1.1.2 Software Paths, Menus, and Tabs 1.1.3 Software Buttons 1.1.4 Images of Software Screens 1.1.5 Analyzer Modes 1.1.6 Notation of Units...
For other precautions refer to CHAPTER 2, Precautions, Installation and Specifictions in the AU5800 User's Guide. 1.1.2 Software Paths, Menus, and Tabs A software path is a sequence of options selected in the software interface in the order indicated.
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Introduction 1.1 Typographical Conventions Used in this Guide Figure 1.1 1. Selectivity Check tab B00418AA...
Introduction 1.1 Typographical Conventions Used in this Guide 1.1.3 Software Buttons All buttons that appear in the software interface are part of a single procedural step and appear in a font. Some software buttons have a corresponding Bold function key that can be accessed on the keyboard instead of using the mouse. They appear in a font with the function number in between parenthesis.
1.1.6 Notation of Units In this guide units are denoted with the International System of Units (SI). 1.1.7 Trademarks The company names and product names used in this AU5800 Quick Response Guide are registered trademarks of their respective companies. B00418AA...
CHAPTER 2 System Overview 2.1 Hardware Overview 2.2 Software Chart 2.3 Navigating Through the Software 2.3.1 How to Select Menus and Items 2.3.2 How to Exit Menus 2.3.3 Entry Status Display 2.3.4 Menus, Tabs, Buttons and Function Keys 2.4 Software Description 2.4.1 Organization of Operation Window 2.4.2 Main Button Bar 2.4.3 Home Button Outline...
7. R1 and R2 syringes 3. ON, EM STOP, RESET buttons 8. Wash solution tank 4. Computer 9. DI water tank 5. ISE unit TIP The rack feeder unit will look different when the AU5800 is connected to a laboratory automation system. B00418AA...
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System Overview 2.1 Hardware Overview Figure 2.2 Top View 1. Rack collection trays 11. Wash nozzle unit 2. Rack set trays 12. R2 mix unit 3. Rack buffer unit 13. Lamp 4. Priority rack set unit 14. R2-2 reagent probe 5.
System Overview 2.2 Software Chart 2.2 Software Chart Main Menu Routine Calibration Calibration Monitor Start Condition QC Monitor ► Daily Chart Calibration Verification ► Reagent ► Day to Day Chart Calibration Verification Reagent Management Twin Plot Chart Material Parameters Reagent Inventory QC Data Review Reagent Consumption Rack Requisition...
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System Overview 2.2 Software Chart Parameters Maintenance System Common Test Parameters User Maintenance Online ► ► Test Name Analyzer Maintenance Format ► Profile ISE Maintenance Requisition Format PROService Group of Tests Load Reagent Parameters List Format Specific Test Parameters ► Alarm Log Comment Masters Repeat Parameters...
System Overview 2.3 Navigating Through the Software 2.3 Navigating Through the Software The system is controlled using the standard windows graphical user interface (GUI). 2.3.1 How to Select Menus and Items 2.3.2 How to Exit Menus 2.3.3 Entry Status Display 2.3.4 Menus, Tabs, Buttons and Function Keys 2.3.1 How to Select Menus and Items •...
System Overview 2.3 Navigating Through the Software 2.3.4 Menus, Tabs, Buttons and Function Keys • Select menus, tabs, buttons, and function buttons/keys to open additional windows. Figure 2.3 1. Menus 3. Buttons 2. Tabs 4. Function Buttons/Keys B00418AA...
System Overview 2.4 Software Description 2.4 Software Description The system is controlled using the standard windows graphical user interface (GUI). 2.4.1 Organization of Operation Window 2.4.2 Main Button Bar 2.4.3 Home Button Outline 2.4.4 Menu List Overview 2.4.5 User Menu Overview 2.4.6 Using the Online Help B00418AA...
System Overview 2.4 Software Description 2.4.1 Organization of Operation Window This interface is composed of three main window display areas. Figure 2.4 A. Main button bar Home Menu List User Menu Help Buttons to display the main menus ( ) and access Start Pause Feeder Stop...
Select this button to display the Menu List menu. Menu List Select this button to display the User Menu set by the user. Refer to 4.10.6 Program a User Menu in the AU5800 User’s Guide. User Menu Displays the current mode. The time to completion displays for some maintenance procedures.
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System Overview 2.4 Software Description Button Actions Select this button to stop analysis. In Stop mode, select this button to return the system to Standby mode. Stop/Standby Select this button to display online operational help. Help Select this button to log out and log in a user. Logout Select this button to perform an End Process.
System Overview 2.4 Software Description 2.4.3 Home Button Outline The system can be navigated by using the main buttons. This is the screen to check system messages relating to sample status, analyzer status or unit condition. Jump buttons provide direct menu access to the most frequently used menus to simplify software navigation.
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System Overview 2.4 Software Description Menu buttons Menu Button Action Sample Status Displays the sample status under analysis, time to completion, and results. Analyzer Status Displays the analyzer status and temperatures. Jump buttons Jump buttons provide direct menu access to the most frequently used menus to simplify software navigation.
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System Overview 2.4 Software Description Message Displays messages regarding system conditions that could affect analysis results. Color-coding indicates the level of the message: Red: Analysis cannot be started on a unit until the message is addressed. Orange: Analysis can be started. Review the message carefully and take appropriate action.
System Overview 2.4 Software Description 2.4.4 Menu List Overview Select Menu List to access Routine Calibration Parameters , and Maintenance System TIP Each menu in the left column has a list of sub-menus in the right column. B00418AA 2-15...
A user-defined list of the common menus operators use. • User-defined menu names can be created. • Select , then select the required menu for direct access. User Menu Refer to 4.10.6 Program a User Menu in the AU5800 User's Guide, chapter 4 for more information. 2-16 B00418AA...
System Overview 2.4 Software Description 2.4.6 Using the Online Help Help windows are available for the operator to access the AU5800 User’s Guide, maintenance videos, and alarm descriptions. TIP To stop the audible alarm select Alarm Clear. Select Alarm Clear a second time to clear the alarm message from the screen.
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There are four types of help as shown in the following table. Type of Help Displayed Description Help Displays the PDF version of the AU5800 User's Guide and Maintenance videos. Select the Help button. Alarm List Displays alarm descriptions and corrective actions.Select Alarm List to display the Alarm List window.
CHAPTER 3 Daily Start Up This chapter describes how to perform the daily start up procedures. 3.1 Start the System 3.1.1 Turn On the System 3.1.2 Set the Start Conditions 3.2 Perform Daily Maintenance 3.3 Check Analyzer Status 3.4 ISE (Optional Unit) Start Up 3.4.1 Check the ISE Reagent 3.4.2 Perform ISE Daily Maintenance 3.5 Check Reagent Status...
Refer to 4.11 Program a User Name and Password in the AU5800 User's Guide. There is an auto login feature available if passwords are not in use. Refer to 4.11.3 Security Settings in the AU5800 User's Guide, chapter 4. B00418AA...
Daily Start Up 3.1 Start the System a. Enter the User Name. b. Enter the Password. c. Select . The New Index window opens. TIP If the system was shut down without performing an End Process, the System Start window to retrieve the Database appears. Select OK.
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Daily Start Up 3.1 Start the System B00418AA...
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Daily Start Up 3.1 Start the System TIP Index Displays the current index. Group of Tests Selects the group of on-board tests to perform. Operator Name Enter the operator name, or select the Select button to select a name from the comment master list. Start Sample No.
• Inspect the stability of the upper cover For details on performing daily maintenance inspections, refer to 8.3 Daily Maintenance in the AU5800 User's Guide, chapter 8. CAUTION To prevent injury or infection be sure to wear protective gloves and other protective equipment when performing maintenance.
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Daily Start Up 3.3 Check Analyzer Status Review Analyzer Status: Select Home > Analyzer Status For details on , refer to 7.2.2 Analyzer Status Menu in the Analyzer Status AU5800 User's Guide, chapter 7. B00418AA...
Daily Start Up 3.4 ISE (Optional Unit) Start Up 3.4 ISE (Optional Unit) Start Up Perform ISE start up and daily maintenance procedures. 3.4.1 Check the ISE Reagent 3.4.2 Perform ISE Daily Maintenance 3.4.1 Check the ISE Reagent Open the front panel of the ISE unit. Verify the ISE reagents are within the 90-day onboard stability.
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6. Reference solution bottle (for Cell 2) For information about loading ISE reagents, refer to 8.19.5 Replacing ISE Reagents in the AU5800 User's Guide, chapter 8. TIP There is a Buffer, MID Standard, and Reference reagent bottle dedicated to each flowcell (Cell1 and Cell2).
• ISE Calibration For details on performing ISE daily maintenance, refer to 8.12 ISE Daily Maintenance in the AU5800 User's Guide, chapter 8. CAUTION To prevent injury or infection be sure to wear protective gloves and other protective equipment when performing maintenance.
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Daily Start Up 3.5 Check Reagent Status B00418AA 3-11...
Daily Start Up 3.5 Check Reagent Status Select a sample type from the “Type” drop-down list. View the Reagent Status box in the upper left hand corner of the Main tab. The Display and Background color indicate the status of the reagent compartments and the reagent volume check.
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Tests highlighted in green are below the Expected Volumes. A day of the week displays the current day of the week. Refer to 7.5.5 Reagent Inventory in the AU5800 User's Guide, chapter 7. Select Reagent Check (F5) Check the box for each unit to perform the reagent check.
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Daily Start Up 3.5 Check Reagent Status TIP Reagent Check (F5) options: • Check all positions Checks the remaining volume of reagent at all positions, including the bottle positions outside of the R1/R2 compartments and sample probes. This includes the bottles by the sample probe for the ISE unit.
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Daily Start Up 3.5 Check Reagent Status If reagent is short or empty, load additional reagent and perform a reagent check again. 3.5.2 Review Detailed Information Reagent Status Home > Reagent Management > Main Figure 3.2 Display Background Color Description Accessible Light blue Accessible displays if reagent bottles can be loaded.
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Daily Start Up 3.5 Check Reagent Status Test Status Home > Reagent Management > Main Figure 3.3 Display Display Description Color Displays test names in the output order that has been set in the Test name 1 Group. ISE and non dedicated LIH reagents are not displayed. Test name 2 In case of 2-tests/1-reagent *, another test name is displayed.
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Daily Start Up 3.5 Check Reagent Status TIP When the analyzer has turned on after an End Process, all tests initially show <30 shots without a volume indicator bar. Select Reagent Check (F5) > Check all positions to determine the number of shots (tests) remaining.
If the reagent volume is insufficient, on-board stability has expired, or the reagent is expired, remove the old reagent bottles and replace them with a new set. CAUTION Never add new reagent to existing reagent in Beckman Coulter reagent ID bottles. 3-18 B00418AA...
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Daily Start Up 3.5 Check Reagent Status Reagent preparation Prepare any necessary reagent according to the manufacturer's instructions for use. Most reagents with a reagent ID are liquid and ready for use. There are 4 types of reagent bottles that can be used with this system. •...
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Daily Start Up 3.5 Check Reagent Status Loading reagents in the R1 and R2 refrigerators WARNING • Condensation can form on the walls of the reagent compartment, in the bottle neck or label area. If condensation is present, remove it using a dry paper towel. •...
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Daily Start Up 3.5 Check Reagent Status Figure 3.5 Reagent Refrigerator Top-down View 1. Partition plate 4. Reagent bottle (30 mL) 2. Reagent bottle (60 mL) 5. Fixed partition 3. Reagent bottle (15 mL) 6. Adapter A maximum of 54 bottles can be placed in the R1 and R2 refrigerators. If 180 mL bottles are used, the number of bottles that can be placed in R1 and R2 is reduced.
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Daily Start Up 3.5 Check Reagent Status Figure 3.6 1. Reagent refrigerator 2 (R2) cover 2. Reagent refrigerator 1 (R1) cover Place reagent bottles in the R1 and R2 refrigerators. TIP Place R1 bottles in the R1 refrigerator, and R2 bottles in the R2 refrigerator on the correct unit(s).
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ID manually. For details on ID editing, refer to 6.10 Edit a Reagent ID CHAPTER As Needed Tasks. For precautions on filling bottles with reagent or Wash Solution, refer to 5.5.3 Reagent Replacement in the AU5800 User's Guide, chapter 5. B00418AA 3-23...
Daily Start Up 3.6 Perform Calibration 3.6 Perform Calibration Requisition reagent blanks and calibrations. Automatic reagent blank and calibration requisition occurs with the following conditions: • Reagent blank or calibration Expired • Reagent blank or calibration Expired Soon • New bottle or lot number of reagent if Advanced Calibration is in use •...
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Daily Start Up 3.6 Perform Calibration Requisition and run reagent blanks and calibrators Select Home > Rack Requisition > Calibration B00418AA 3-25...
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Daily Start Up 3.6 Perform Calibration Select from the "Type" drop-down Serum Urine Other-1 Other-2 list. “Cuvette” defaults to display all tests assigned to the Inner and Outer cuvette wheels. Select Inner or Outer to display only those tests assigned to the Inner or Outer wheel. “Unit No.”...
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Daily Start Up 3.6 Perform Calibration A one-point calibration correction can be requisitioned and performed if the test is a multi-point calibration, and 1-Point Calibration Point is programmed in Calibration Parameters. If the system consists of only one unit, “Unit No.” is not displayed.
Quality control analysis is used for confirming the system performance. It is necessary for all diagnostic equipment. Check the performance of the AU5800 regularly by analyzing QC samples. Each laboratory should establish its own control frequency. QC samples should be tested each time patient samples are tested and each time calibration is performed.
Daily Start Up 3.7 Perform Quality Control (QC) TIP A default QC requisition can be programmed in Menu List > Parameters > Common Test Parameters > Profile > QC for each sample type and Group in use. Specific profile numbers are designated for each sample type and Group: Profile Number Sample Type/Group...
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Daily Start Up 3.7 Perform Quality Control (QC) Requisition and run QC Select Home > Rack Requisition > QC 3-30 B00418AA...
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Daily Start Up 3.7 Perform Quality Control (QC) Select from the "Type" drop-down Serum Urine Other-1 Other-2 list. “Cuvette” defaults to display all tests assigned to the Inner and Outer cuvette wheels. Select Inner or Outer to display only those tests assigned to the Inner or Outer wheel.
Daily Start Up 3.8 Start Analysis Select to register the QC requisition. Entry (F2) Select to view the control(s), racks, and Display Cup Set (F5) positions required for the control(s). Load the controls according to the list in the correct green racks. Select Close to close the window.
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Daily Start Up 3.8 Start Analysis Verify all units have a check in the box indicating the unit will be started. If the box is gray and inaccessible, review the error list and perform necessary corrective actions before starting. Remove the check from the box if analysis is not required on a unit, and the unit will not start.
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Daily Start Up 3.8 Start Analysis 3-34 B00418AA...
CHAPTER 4 Processing Samples This chapter describes how to prepare racks and samples for analysis, requisition for samples, process emergency samples, perform repeat analysis, print results, and transfer data to a host computer. 4.1 Rack and Sample Cup/Tube Preparation 4.1.1 Rack Types 4.1.2 Place the Sample Cups/Tubes in a Rack 4.2 Place a Rack on the Set Tray 4.3 Place a Set Tray on the Analyzer...
The racks come in six different colors. Each rack color has a specified purpose or application. Racks are placed on the AU5800 rack set trays. A maximum of 20 racks can be placed on each of the two rack set trays provided. Both of the rack set trays can be placed on the rack feeder unit for a maximum of 40 racks on the unit.
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1 in the blue rack. The blue rack requires a rack ID. Rack ID 0001 applies to all units. If multi-rack ID is in use (1001, 2001, 3001 and/or 4001) the first digit designates the unit for the rack. Refer to 6.2.1 Multi-rack ID in the AU5800 User's Guide, chapter 6. Yellow Used for creation of calibration curves.
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Processing Samples 4.1 Rack and Sample Cup/Tube Preparation Magnet Color Rack Application Green Used for analysis of QC samples Control material is programmed to a QC number (1 to 100). Control numbers 1 to 100 are identified by the rack ID and position.
For details on assigning rack ID numbers for specific sample types, refer to 4.10.2 Program the Rack Number Limit in the AU5800 User’s Guide, chapter 4. WARNING Insert the sample tubes/cups properly into the rack.
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Processing Samples 4.1 Rack and Sample Cup/Tube Preparation Figure 4.1 1. Small diameter tube with ID 2. Large diameter tube with ID 3. Hitachi cup 4. Large diameter tube with ID + Hitachi cup WARNING Use only NE racks. TIP An NE rack has a window on the side to facilitate setting different sample cup types in the rack and not compromise barcode readability.
Processing Samples 4.2 Place a Rack on the Set Tray 4.2 Place a Rack on the Set Tray Four identical trays are provided with the system. Two function as rack set trays and are placed on the rack set unit. Two function as collection trays and are placed on the rack collection unit.
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Processing Samples 4.2 Place a Rack on the Set Tray Figure 4.3 1. Set the sample cup with DI water. 6. Blue rack 2. Set in order of smallest calibrator rack ID. 7. Yellow rack 3. Set in order of smallest control rack ID. 8.
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QC. The unit number for each rack is on the multi-rack ID label. For details on multi-rack ID, refer to 6.2.1 Multi-rack ID in the AU5800 User's Guide, Chapter 6. Slide the rack stabilizing bar forward to the rack.
LED is off. If the tray is removed while the orange LED is lit, an injury may result or the rack may topple over. For details of LED functions, refer to 3.4.3 LEDs and Operation Buttons (Rack Feeder Unit) in the AU5800 User's Guide, chapter 3. 4-10 B00418AA...
Processing Samples 4.4 Add Racks Directly to the System Set the prepared trays in the rack set unit and the rack collection unit. Figure 4.5 1. Rack stabilizing bar 4. Set tray1 2. Collection tray1 5. Set tray2 3. Slide the rack stabilizing bar forward 6.
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Processing Samples 4.4 Add Racks Directly to the System Figure 4.6 1. Rack collection unit Orange LED 7. Rack set unit 2 Green LED 2. Sample ID barcode reader 8. Rack set unit 2 Orange LED 3. Rack ID barcode reader 9.
Processing Samples 4.5 Add Racks to the Priority Rack Set Unit Add racks to the rack set unit when the green LED is on. If the rack set unit orange LED is blinking, press the Rack Set/Diag button to stop moving existing racks. When the orange LED is off (the Rack Set/Diag button is blinking), new racks can be loaded.
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Processing Samples 4.5 Add Racks to the Priority Rack Set Unit Verify the orange LED on the priority rack set unit is not flashing. This indicates racks are in progress. Wait until the LED stops flashing. Figure 4.7 1. Orange LED Open the cover of the priority rack set unit.
Processing Samples 4.6 Requisition for Routine and Emergency Samples 4.6 Requisition for Routine and Emergency Samples Sample information and the tests requested to run on a sample (requisitions) must be entered for each sample to be analyzed. The system uses these requisitions to process each sample. To run an emergency sample, requisition the sample as Emergency, and place the sample in a red rack.
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Processing Samples 4.6 Requisition for Routine and Emergency Samples 4-16 B00418AA...
Processing Samples 4.6 Requisition for Routine and Emergency Samples At “Sample Kind”, select to select Switch Routine Emergency samples. Routine: Normal analysis in a white rack Emergency: Priority analysis in a red rack Select from the "Type" drop-down Serum Urine Other-1 Other-2 list.
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Processing Samples 4.6 Requisition for Routine and Emergency Samples When selecting a profile, perform one of the following operations. a. Select Profile to open the profile window and select a profile (or multiple profiles). b. Use the keyboard to enter a profile number into Profile and press Enter Menu List >...
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Processing Samples 4.6 Requisition for Routine and Emergency Samples Select Exit (F1) TIP Pending List (F4) View a list of samples that have been requisitioned, but not yet processed on the analyzer. Select a Sample No. or Sample ID number, then select to view the specific sample requisition.
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Processing Samples 4.6 Requisition for Routine and Emergency Samples 4.6.2 Enter Batch Requisitions To perform the same tests on a number of samples, enter the requisitions in a single batch. Tests requisitioned for one sample will be requisitioned for all samples in the batch.
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Processing Samples 4.6 Requisition for Routine and Emergency Samples B00418AA 4-21...
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Processing Samples 4.6 Requisition for Routine and Emergency Samples Select Start Entry (F1) Select the tests for batch requisition for one sample. For details on manually requisitioning tests, refer to 4.6.1 Enter Manual Requisitions in this chapter. Select . The Batch Entry window opens. Batch Entry (F3) Select to enter the actual number of samples...
Batch: the system waits for a user to instruct it to download and execute requisitions. For information on how to configure these modes, refer to 4.12 Program Online Parameters in the AU5800 User’s Guide, chapter 4. Select Home > Rack Requisition > Sample...
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Processing Samples 4.6 Requisition for Routine and Emergency Samples 4-24 B00418AA...
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Processing Samples 4.6 Requisition for Routine and Emergency Samples Select Batch Req. from Host (F7) Select the sample kind and type from the "Sample Kind" drop-down list. enter the starting and ending sample numbers to Sample No. download from the host computer. Select .
Processing Samples 4.7 Processing Emergency Samples 4.7 Processing Emergency Samples Red racks are used for analysis of emergency samples. Emergency samples can be processed with priority over routine samples. Emergency samples are identified by an “E” sample number prefix in the requisition and sample results.
Processing Samples 4.7 Processing Emergency Samples 4.7.3 Requisition for an Emergency Sample Select Home > Rack Requisition > Sample > Test Requisition Select at “Sample Kind” to change from Routine to Switch Emergency. The next available “Sample No.” displays, or a sample number can be entered at Select Sample No.
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Processing Samples 4.7 Processing Emergency Samples Refer to 4.6.1 Enter Manual Requisitions, 4.6.2 Enter Batch Requisitions, and 4.6.3 Download Requisitions from a Host Computer procedures to select the tests and complete the emergency requisition. CAUTION The “Sample Volume (uL)” does not include the dead volume. TIP Place the red rack in front of white racks on the rack set tray to give the red rack priority, or place the red rack on the priority rack set unit.
CHAPTER 5 Monitoring Results This chapter describes how to review results using the software or printed reports, and check the results for flags and alarms. 5.1 Monitor Results 5.2 Identifying Sample Types by Sample Data Prefix 5.3 Sample Status Menu 5.4 Reports 5.5 Check for Error Flags and Alarms 5.5.1 Check for Error Flags...
Alarm List taken for any alarms generated. Refer to 7.4 Check Results in the AU5800 User's Guide, chapter 7, for more information on monitoring reagent blank, calibration, QC, and sample results. For more information on troubleshooting and corrective actions, refer to 11.3 Troubleshooting the System - Reagents, Calibrators, Quality Control and Samples in the AU5800 User's Guide, chapter 11.
Monitoring Results 5.3 Sample Status Menu 5.3 Sample Status Menu Select Sample Status to view sample information, time to completion, and results. Select Home > Sample Status Figure 5.1 menu displays: Status • Sample No.: The sample number displays highlighted in the color of the rack.
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Monitoring Results 5.3 Sample Status Menu • Results: Displays the time to completion when the sample is in process, or “Error” if the sample is complete and an error flag is present. Highlight a sample, then select to view detailed sample Detail information.
AU5800 User's Guide, chapter 4, or contact Beckman Coulter Technical Services for more information. Results can print realtime for reagent blank, calibration, QC, and/or samples. Refer to 4.14.1 Format the Realtime Reports and Lists in the AU5800 User's Guide, chapter 4. Results can be printed from Home >...
For a list of error flags and the flag definition, refer to 8.3 Review Error Flags in chapter 8. For details on error flags, refer to CHAPTER 9, Error Flags in the AU5800 User's Guide. 5.5.2 Check for Alarms Check if any alarms occurred during analysis: on the lower, right corner of the screen).
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Monitoring Results 5.5 Check for Error Flags and Alarms “Level” shows the alarm level by numbers and colors. Level Background Color Extent Level 1 A fatal system abnormality exists. Level 2 Yellow An abnormality influencing the data exists. Level 3 Green There is no system abnormality.
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Monitoring Results 5.5 Check for Error Flags and Alarms From this window the following options can be performed: • : Prints a list of all alarms. Print • : Returns the display to the most recent alarms. Refresh • : Display a description of the alarm and corrective actions. Help •...
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Monitoring Results 5.5 Check for Error Flags and Alarms 5-10 B00418AA...
CHAPTER 6 As Needed Tasks This chapter describes how to program a new test on the system and contains a detailed description of all the Parameters menus. 6.1 Program a New Test 6.2 Create a Profile 6.2.1 Create a Sample Profile 6.2.2 Create a Reagent Blank or Calibration Profile 6.2.3 Create a QC Profile 6.3 Program Calibrator Concentrations...
6.15 Save or Load Parameters 6.15.1 Saving or Loading Parameter Files 6.16 Disable a Test 6.1 Program a New Test Program a new test using the current parameter sheet. Refer to the AU5800 User's Guide, CHAPTER 4, Parameters for detailed Parameter menu descriptions. Select Menu List >...
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As Needed Tasks 6.1 Program a New Test • Long Name: (Optional) Enter a maximum of 20 characters. • Reagent ID: Enter the first 3 digits of the reagent ID, or refer to the parameter sheet for the reagent ID 3-digit code. •...
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As Needed Tasks 6.1 Program a New Test Select Menu List > Parameters > Specific Test Parameters display the tab. General • Select Edit (F1) • Select the test from the "Test Name" drop-down list. • Select from the "Type" drop- Serum Urine Other-1...
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As Needed Tasks 6.1 Program a New Test Select Range • Verify the test name at the "Test Name" drop-down list. • Select Edit (F1) • Select . Select for the appropriate Set Decimal Places (F5) decimal place for the results. Select Close •...
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Calibration, Lot Calibration, and Stability. — Counts (replicates): Enter a number from 1 to 4. For more information, refer to 4.5.3 Calibration Specific Menu in the AU5800 User’s Guide. — Calibrator: Select the calibrator material. — Conc: Enter the calibrator concentration from the package insert.
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As Needed Tasks 6.1 Program a New Test Select Menu List > Parameters > QC Parameters > Controls • If it is necessary to program new control material: — Select Edit (F1) — Select an available Control No. and Type. —...
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TIP Contamination can be prevented by programming sensitive tests to different sides of the cuvette wheel (inner or outer) or on different analyzer units without affecting analyzer throughput. Contact Beckman Coulter Technical Services for assay specific contamination parameter information and test assignment to cuvette wheel and unit.
As Needed Tasks 6.2 Create a Profile Run the test to verify the programming of the new test(s). • Load the reagent and any required cleaning solution on the analyzer. • Perform a reagent volume check. • Verify that calibration is requisitioned for the new test(s). •...
As Needed Tasks 6.2 Create a Profile 6.2.1 Create a Sample Profile Select Menu List > Parameters > Common Test Parameters > Profile > Sample Select Edit (F1) Select from the "Type" drop-down Serum Urine Other-1 Other-2 list. At the “Profile Name” drop-down, enter a profile number 0 to 99. 6-10 B00418AA...
As Needed Tasks 6.2 Create a Profile , enter a profile name with a maximum of 20 Profile Name characters. Select the test(s) to be included in the profile. Selected tests appear in blue. Select Confirm (F1) 6.2.2 Create a Reagent Blank or Calibration Profile TIP The programming in Calibration Specific determines the calibration options available in Calibration Options (F5).
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As Needed Tasks 6.2 Create a Profile Select Edit (F1) Select from the "Type" drop-down Serum Urine Other-1 Other-2 list. At the “Profile Name” drop-down, enter a profile number 0 to 99. , enter a profile name with a maximum of 20 Profile Name characters.
As Needed Tasks 6.2 Create a Profile 6.2.3 Create a QC Profile TIP QC profiles 87 to 98 are the default QC profiles that will be automatically requisitioned in Home > Rack Requisition > QC. The QC profile numbers 87 to 98 correspond to a specific Group and sample type: •...
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As Needed Tasks 6.2 Create a Profile Select Menu List > Parameters > Common Test Parameters > Profile > QC Select Edit (F1) Select Serum Urine Other-1 Other-2 from the "Type" drop-down list. At the “Profile Name” drop-down, enter a profile number 0 to 99. , enter a profile name with a maximum of 20 Profile Name characters.
IT IS CRITICAL ALL MULTI-POINT CALIBRATOR VALUES WERE ENTERED CORRECTLY. For details on calibration specific test parameters, refer to 4.5.3 Calibration Specific Menu in the AU5800 User’s Guide, chapter 4. Select Menu List > Parameters > Calibration Parameters > Calibrators Select .
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As Needed Tasks 6.3 Program Calibrator Concentrations Select . The Set Conc Value window opens. Set Conc Value (F5) The concentration value(s) of the selected calibrator display. To display or edit a different calibrator, select the calibrator name from the "Calibrator" drop-down list. Enter the concentration values (Conc) for each test (Test Name) in the calibrator.
6.4 Program Preset QC Mean and Range Use this procedure to verify and change the QC mean, standard deviation, and range. For detailed information on QC parameters, refer to 4.6.2 QC Specific Menu in the AU5800 User's Guide, chapter 4. Select >...
Follow laboratory protocols for inspecting the DI water system and main water valve. TIP The ability to perform “Auto Preparation” is enabled for each day of the week in System Maintenance by Beckman Coulter Technical Services. The three auto preparation options are: •...
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As Needed Tasks 6.5 Program the Automatic Start Up Function Select Menu List > System > System Condition > Auto Power On Select Edit (F1) Select the check box of the desired day to perform the “Auto Start Up Time” function. Hours Minutes from the drop-down list.
As Needed Tasks 6.6 Program a User Menu To perform Auto Preparation, select the “Auto Preparation” check box. Repeat steps 3 to 5 for each day to be set. Select . The programming is registered. Confirm (F1) 6.6 Program a User Menu The User Menu function allows the selection of up to 16 menus most frequently used by the operator.
As Needed Tasks Replace with User Menu.tif 6.6 Program a User Menu 6.6.1 Edit the User Menu Select Menu List > System > User Menu Select . The next available menu to program changes from Edit (F1) the gray box to a blue button. Select the blue button.
As Needed Tasks 6.6 Program a User Menu From the “Select Screen” drop-down list, select the menu to put in the User Menu list. , enter the user-defined menu name. Up to 28 Display Data characters can be entered on each of the two lines. Select Entry Select...
For details on how to program a report format, refer to 4.14.1 Format Realtime Reports and Lists in the AU5800 User’s Guide, chapter 4. 6.7.1 Print Sample Data Reports TIP The report format must be programmed in advance.
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As Needed Tasks 6.7 Print Results The data from the current index displays. All samples are selected to print (highlighted in blue). Use Select All Samples Deselect All Samples as required. Select to search for data by an Index Range, Sample Search (F3) Number(s), Sample ID, Data Not Transferred to Host, or Data Not Printed.
As Needed Tasks 6.7 Print Results Select . The Print window opens. Print (F8) Select the “List Format”. , the Operator Name entered in displays. Reporter Start Condition If necessary, enter a new name or use to enter a pre- Select programmed comment.
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As Needed Tasks 6.7 Print Results Select the index of the RB/CAL/QC data to search from the “Index” drop-down list. Select the “Sample Kind” check box of the samples desired. 6-26 B00418AA...
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As Needed Tasks 6.7 Print Results For “Search Sample No.”, enter the sample number range to print, or leave the “*” to print all samples. TIP If the “Search Sample No.” is left blank or empty no search criteria is used to perform the search. For “QC/Cal.
As Needed Tasks 6.8 Transfer Data to Host Computer 6.8 Transfer Data to Host Computer 6.8.1 Sample Data Select Home > Sample Manager > Main 6-28 B00418AA...
As Needed Tasks 6.8 Transfer Data to Host Computer The data from the current index displays with all samples selected (highlighted in blue). Use Select All Samples Deselect All , or select Sample No. (s) individually to transfer to the host Samples computer.
As Needed Tasks 6.9 Fix (Assign) a Reagent Position From the RB/CAL/QC screen, select the samples and criteria to transfer. Select Transfer to Online (F7) to display the Transfer to Online window. Select . Online transfer is performed. TIP Select Online Transfer Stop (F7) to stop the transfer. 6.9 Fix (Assign) a Reagent Position Fix (assign) reagents that are not barcoded.
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As Needed Tasks 6.9 Fix (Assign) a Reagent Position Select Position Setting (F2) Select . A blue box with an “*” displays to Fixed Reagent then Close the left of “Pos.” for fixed reagents. Select Edit (F1) a. Select the “Test Name”, “Type” (R1 or R2), and “Bottle Size”. b.
As Needed Tasks 6.10 Edit a Reagent ID Verify a blue box with an “*” displays to the left of “Pos.” for fixed reagents. Select to update Reagent Check (F5) > Check Specified Positions the shot count. 6.10 Edit a Reagent ID Edit the reagent ID after a reagent ID read error occurs on a barcoded reagent bottle.
As Needed Tasks 6.11 Reagent Inventory Type in the 20-digit reagent ID from the reagent bottle. Select . The on-board stability, expiration, lot number, and bottle number updates with a “No Volume to Process” comment. Select to update Reagent Check (F5) > Check Specified Positions the shot count.
As Needed Tasks 6.11 Reagent Inventory 6.11.1 Auto Calculation of Reagent Inventory Select Home > Reagent Management > Reagent Inventory > Auto TIP Auto calculations can be entered one day in advance. Display Range (F3) Set the starting and ending indexes to calculate the reagent usage.
As Needed Tasks 6.11 Reagent Inventory Select the start index and the end index from each drop-down list. Select . The number of tests selected within the index range is displayed on the list. Select R1 Volume R2 Volume . The reagent consumption is automatically calculated based on an actual result and then displayed on the list by mL.
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As Needed Tasks 6.12 Reagent Consumption Select . The window changes to reference mode. Confirm (F1) Select . The reagent consumption input is R1 Volume R2 Volume automatically calculated based on entered test numbers and then displayed on the list by mL. Select from the “Decision”...
As Needed Tasks 6.12 Reagent Consumption Shot Total TIP The is the cumulative number of tests run on the analyzer from the installation of the instrument. Select Serum Urine Other-1 Other-2 from the "Type" drop-down list. Select Display Range (F3) Select the start index and the end index from each drop-down list.
To reflect the editing of contents in the cumulative values, update the cumulative values. Refer to 4.6.2 QC Specific Menu in the AU5800 User's Guide, chapter4 for updating and calculating cumulative QC values.
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As Needed Tasks 6.13 Edit Quality Control Data Select Menu List > QC > QC Data Review > Main Select the required index from the “Index” drop-down list. Place a check mark by QC in the Sample Kind column. Set the search range by entering the Search Sample No., the QC No., and the Search Control ID.
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As Needed Tasks 6.13 Edit Quality Control Data Select the tab to edit QC by QC sample number. Sample • Select Edit (F1) • All units default to checked. Remove the check mark from a unit as required. • To edit a test(s) in the sample, select the Result or Data Flag to edit.
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As Needed Tasks 6.13 Edit Quality Control Data • Select Edit (F1) • All units default to checked. Remove the check mark from a unit as required. • Select the test to edit or delete from the “Test Name” drop-down list.
As Needed Tasks 6.14 Save Data to External Media 6.14 Save Data to External Media Save analysis data to external media for a backup of data, or to transfer the data to another computer. Data is saved to an “AU Data” folder. The data files are identified by the index.
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As Needed Tasks 6.14 Save Data to External Media Select to save routine and emergency samples, Patient Repeat Run to save repeat samples, or RB/CAL/QC to save reagent blank, calibration, or QC samples. Select the starting and ending index from the “Index” drop-down list. Check the Sample Kind to save.
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As Needed Tasks 6.14 Save Data to External Media The Perform window opens after searching for the data. Verify the Total Output Samples and <Number of Output Sample> information. Select from the [Media Select] CD-R External Memory Unit options. Select .
As Needed Tasks 6.15 Save or Load Parameters 6.15 Save or Load Parameters The system can save or load parameters to a backup folder on the hard drive or external media. 6.15.1 Saving or Loading Parameter Files Select Menu List > System > Data Management > File Management B00418AA 6-45...
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As Needed Tasks 6.15 Save or Load Parameters , select Operation Save Files to HD Load Files from HD Save , or Files to External Media Load Files from External Media TIP Saving or loading files to “HD” is a backup folder on the hard drive.
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As Needed Tasks 6.16 Disable a Test The Perform window opens when the operation is complete. Figure 6.1 Select . Remove the external media. 6.16 Disable a Test Specific tests can be selected to stop analysis (disable) even when the test has a requisition.
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As Needed Tasks 6.16 Disable a Test Select Home > Start Condition Select . The Disable window opens A list of tests to Disable (F7) disable is displayed. 6-48 B00418AA...
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As Needed Tasks 6.16 Disable a Test Select the unit. Select the test(s) to be disabled. Tests that are disabled are highlighted in orange, and the unit displays “Disabled”. Select . Test selections are registered, and the display returns to Start Condition B00418AA 6-49...
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As Needed Tasks 6.16 Disable a Test 6-50 B00418AA...
CHAPTER 7 Analyzer Modes This chapter describes the analyzer modes, how to perform a system shutdown ), and how to stop analysis or emergency stop the system. End Process 7.1 Analyzer Modes 7.2 Bypass Warm Up to Standby Mode 7.3 System Shutdown (End Process) 7.4 Transfer the Analyzer to Stop Mode 7.4.1 Stop Analysis 7.4.2 Return to Standby Mode...
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Analyzer Modes 7.1 Analyzer Modes 7.1 Analyzer Modes Figure 7.1 1. Mode Display Area The system modes that can display in the “Mode Display Area” are shown below. Mode Contents Initial Displays after the green ON button is pressed. The software loads and the hardware initializes.
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Perform a W1 to clean the sample probe and cuvettes. For details on how to perform a W1, refer to 8.8.13 Perform a W1 Procedure in the AU5800 User’s Guide, chapter 8. B00418AA...
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Analyzer Modes 7.2 Bypass Warm Up to Standby Mode 7.2 Bypass Warm Up to Standby Mode The system requires a 20 minute lamp warm up before analysis can be started if an was performed. If there is a power failure or an emergency End Process stop (EM STOP button on analyzer) was performed, the mode stays in Warm Up for 1.5 hours.
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6.5 Program the Automatic Start Up Function in chapter 6, in this guide. Auto Preparation must be programmed in the System Maintenance menu by Beckman Coulter Technical Services at installation. Auto Preparation can be programmed for the weekly Photocal. B00418AA...
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• Reagents remain in the cuvettes after performing a Stop. Perform a W1 to remove reagents before performing an End Process or resuming analysis. Refer to 8.8.13 Perform a W1 Procedure in the AU5800 User's Guide, chapter 8. B00418AA...
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Analyzer Modes 7.4 Transfer the Analyzer to Stop Mode 7.4.1 Stop Analysis Select during analysis operation. The Stop window Stop/Standby opens with the message “Transfer to Stop Mode?”. Figure 7.2 1. Stop/Standby Select . All analysis operation stops, and the system goes to Stop mode.
Reagents remain in the cuvettes after performing an Emergency Stop. Perform a W1 to remove reagents before performing an End Process or resuming analysis. For details on performing the W1, refer to 8.8.13 Perform a W1 Procedure in the AU5800 User's Guide, chapter 8. B00418AA...
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20-minute lamp warm up time, select Home > Analyzer to return to Standby. Maintenance > Stand By (F4) Perform a W1. For details on performing the W1, refer to 8.8.13 Perform a W1 Procedure in the AU5800 User's Guide, chapter 8. B00418AA...
Troubleshooting For detailed information on troubleshooting the system, including reagents, calibrators, quality control, samples, mechanical problems, and data processor problems, refer to CHAPTER 11, Troubleshooting in the AU5800 User's Guide. 8.1 Using the Online Help 8.2 Review Alarms and Corrective Actions 8.3 Review Error Flags...
Troubleshooting 8.1 Using the Online Help 8.1 Using the Online Help Help windows are available for the operator to access the User’s Guide, maintenance videos, and alarm descriptions. Figure 8.1 1. Help 2. Alarm List B00418AA...
To determine the function of a button, move the mouse pointer over the button and a pop-up message will indicate the button’s function. The AU5800 User's Guide and this Quick Response Guide do not describe alarms or corrective actions. Select Alarm List for alarm help.
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Troubleshooting 8.2 Review Alarms and Corrective Actions “Level” shows the alarm level by numbers and colors. Background Level color Extent Level 1 A fatal system abnormality exists. Level 2 Yellow An abnormality influencing the data exists. Level 3 Green There is no system abnormality. The operation log is displayed.
Check all generated results carefully for error flags and take the appropriate action. For details on error flags, refer to CHAPTER 9, Error Flags. in the AU5800 User's Guide. It is important that the operator reviews each flag as it is generated and identifies the root cause.
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Troubleshooting 8.3 Review Error Flags The following table summarizes the error flags in order of priority: Flag Definition QC result is excluded by user Data edited by user Shortage of cleaning solution for contamination parameters Result has been analyzed with an erroneous cuvette Insufficient reagent detected Insufficient sample detected Clot detected...
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Troubleshooting 8.3 Review Error Flags Flag Definition Unable to calculate concentration Reagent lot number used for sample analysis is different from the lot number used for RB/Calibration Reagent expired No calibration data or expired The latest calibration/RB has not been used Mastercurve used Calibration curve for Prozone data used Result is higher than the dynamic range...
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Troubleshooting 8.3 Review Error Flags Flag Definition A preset number of consecutive QC results fall on one side of the mean Consecutive QC results show steadily increasing or decreasing values Previously-set QC data is out of the permissible range Sample repeated and original results replaced by repeat result Test pending or not analyzed Result has been transferred to LIS (HOST Computer) through on-line communication...
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Index Alarms Hardware, Alarm shots, Host computer, 4-23, 6-29 Analyzer status, Buttons, 2-12, 2-13 ISE daily maintenance, ISE reagents, ISE start up, Calibration Advanced calibration, 3-16, 3-27, 3-31 Perform calibration, 3-24–3-28 Main button bar, Calibrator Maintenance Program calibrator concentrations, 6-15 Daily maintenance, 3-6, 3-10 Measure modes...
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Start up, Quality control Perform QC, 3-28–3-32 Typographical conventions, Rack feeder unit, Rack transfer unit, Units, Racks Blue rack, Green rack, Warnings Red rack, 4-17, 4-26, 4-28 Warnings and cautions, White rack, 4-8, 4-17, 4-28 Yellow rack, Reagent transfer unit, Reagents Reagent check, 3-14...
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Questions and answers
how to program Orange rack for repeat
To program the Orange rack for repeat analysis on the Beckman Coulter AU5800:
- If using Sequential analysis:
Place samples in numeric order based on the repeat run requisition. Do not leave empty spaces in the rack.
- If using Barcode analysis:
Place samples in any position in the rack.
No specific programming steps are required beyond correct sample placement based on the analysis method.
This answer is automatically generated