Following is the relation between delay time and column flush.
(1)
With AUTO STOP at YES
(a)
When YES is selected for COLUMN FLUSH
Analysis 1
Analysis 2
∗ "n" indicates number of analyses specified with autosampler, etc.
Stabilizing time is the time from the end of one analysis until the start of the
next analysis.
Column flushing starts when delay time has ended.
(b)
When NO is selected for COLUMN FLUSH
Analysis 1
Analysis 2
∗ "n" indicates number of analyses specified with autosampler, etc.
Stabilizing time is the time from the end of one analysis until the start of the
next analysis. The pump stops when delay time has ended.
(2)
With AUTO STOP at NO
Analysis 1
Analysis 2
∗ "n" indicates number of analyses specified with autosampler, etc.
The pump remains in liquid delivery status.
Delay time
Gradient end
Analysis 3
Stabilizing time
Analysis 3
Stabilizing time
Analysis 3
Stabilizing time
4 - 29
Column flush
∗
Analysis n
Delay time
∗
Analysis n
Delay time
∗
Analysis n
Pump remains in
liquid delivery status.
COLUMN FLUSH
Pump stop
Pump stop