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The following instructions provide a guide to performing simple tasks on the microscope. Initial sections
(A to D) describe how to insert a sample and obtain an image. Subsequent sections provide information
on more COni~!CX operations such as changing the accelerating voltage or the detecton Sections K and
L provide infonnation on what to do at the end of a session.
Compared with the JEOL 840 SEM there are more variable parameters available to the operator.
Optimum imaging conditions will vary from user to user depending on their samples. It is
advisable that you spend some time attempting to determine which conditions are best for your
sample.
When using this microscope please note the following rules:
RULE 1: DON'T TOUCH A CONTROL IF YOU ARE NOT SURE OF THE
OUTCOME OF THAT ACTION.
RULE 2: NEVER, EVER FORCE ANYTHING BEYOND FINGER STRENGTH
RULE 3: IF IN DOUBT; ASK FOR HELP
A. START-UP
1. Turn on the visual display by turning toggle switch DISPLAY POWER to 1. (These
toggle switches must be pulled out slightly before switching - DO NOT FORCE THEM)
2. Turn the toggle switch SC AIRLOCK VALVE from CLOSED to AUTO (OPEN)- a
few seconds later the green light on the HV off/ready display will come on. If it doesn't please
contact EM staff for help.
B. INSERT SAMPLE
1. Put specimen stub onto specimen holder> inc exact mechanism will vary depending upon
the specimen type. EM Unit staff will advise the best method for your sample when you art
being trained. The specimen holder and stub MUST be set so that so that thtt top of the
specimen is level with or below tIze gauge.
2. Ensure toggle switch on SC AIRLOCK VALVE DISPLAY is set to S.E.C.
3. Press AIR on Sc AIRLOCK VALVE DISPLAY - this will bleed air into the specimen
exchange chamber. It will take 2~3O seconds for the chamber to come to air.
4. Ensure stage settings
(displayed on the specimen chamber).
5. Open specimen exchange chamber door Do not force.
6. Push specimen exchange rod forward slightly so it protrudes through the specimen exchange
chamber door and screw specimen holder on to screw thread of the specimen exchange rod.
PLEASE MAKE SURE YOU DO NOT GET A CROSS THREAD. Pull specimen
exchange rod back into specimen exchange chamber door.
7. Close airlock door
HITACHI 4500-Il INSTRUCTIONS
(X,Y,Z,R
and T) have been return to their default positions
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Related Manuals for Hitachi McDATA Sphereon 4500
Summary of Contents for Hitachi McDATA Sphereon 4500
- Page 1 RULE 3: IF IN DOUBT; ASK FOR HELP A. START-UP 1. Turn on the visual display by turning toggle switch DISPLAY POWER to 1. (These toggle switches must be pulled out slightly before switching - DO NOT FORCE THEM) 2. Turn the toggle switch SC AIRLOCK VALVE from CLOSED to AUTO (OPEN)- a few seconds later the green light on the HV off/ready display will come on.
- Page 2 7. Set magnification to IQOOX. You can do this by manually turning the MAGNIFICATION knob or pressing the PRESET switch on the magnification display. 8. Locate area of interest using X and V stage shifts (you may need to go to low magnification see section I).
- Page 3 4. Wait for the line scan to perform on cycle and expose the film. Exposure is complete when light on the DIRECT switch goes off. 5. Withdraw the film from the cassette. WHAT TO DO WHEN IT ALL GOES DARK - DON'T PANIC: DON'T FIDDLE WITH ANYTHING 1.
- Page 4 Hit ENTER to move $ sign against STIG ALIGN Y Press ENTER again 11. The image will oscillate on the screen, minimise oscillation with ALIGNMENT X and Y knobs. 12. Press PF16 EXIT to finish. CHANGING THE DETECTOR The 4500 has two secondary electron detectors, an upper detector which sits above the specimen and a lower detector which sits to the side of the specimen.
- Page 5 Often it can be difficult to locate the area you want on a specimen. In this case use Low Magnification Imaging, here the lowest magnification is x20. 1. Press LOW MAG switch on display panel (the green light in the switch will glow). This will automatically set the magnification to x500.
- Page 6 FINISHING A SESSION 1. Turn the SC AIRLOCK VALVE from AUTO (OPEN) to CLOSED - the green light on the HV off/ready display will turn off.. 2. Turn off the visual display by turning DISPLAY POWER to O. 3. Fill in log book.