Fractionation - GE AKTA pilot 600 User Manual

4.2

Fractionation

Introduction
Fractionation in ÄKTA pilot 600 system is performed using the outlet valves. Up to five
outlet valves can be installed in the system, giving up to 15 outlets. Outlet 1-Waste is
used by default as a waste outlet.
Fractionation is set in the Phase Properties of the UNICORN method, and can be used
in the following phases:
•
•
•
•
Fractionation settings
Fractionation settings determine the type of fractionation.
Whether and how fractionation will be performed is determined by the three options to
the left of the panel:
Setting
Continue ongoing
Outlet valve frac-
tionation
No fractionation
ÄKTA pilot 600 User Manual 29274559 AA
Column Wash
Sample Application
Conditional Fractionation
Elution
Description
Continues fractionation using the settings from the previous
phase.
Note:
If Stop fractionation at the end of this phase is checked in the
previous phase, fractionation will not continue. If there is no
previous phase with fractionation settings, fractionation will not
be performed.
Performs fractionation according to the specified type and
settings.
Outflow will be directed to outlet 1-Waste.
4 Operation

4.2 Fractionation

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