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1525
Waters 1525 Manuals
Manuals and User Guides for Waters 1525. We have
1
Waters 1525 manual available for free PDF download: Operator's Manual
Waters 1525 Operator's Manual (188 pages)
HPLC Pump
Brand:
Waters
| Category:
Water Pump
| Size: 1.32 MB
Table of Contents
Copyright Notice
2
Trademarks
2
Customer Comments
3
Safety Considerations
3
Considerations Specific to the 1500-Series HPLC Pumps and Column Heater
4
Safety Advisories
5
Operating the Waters 1500-Series HPLC Pump and Options
5
Applicable Symbols
6
Audience and Purpose
6
Intended Use of the Waters 1500-Series HPLC Pump and Options
6
Calibrating
7
Quality-Control
7
ISM Classification
8
ISM Classification: ISM Group 1 Class B
8
EC Authorized Representative
8
Table of Contents
9
1 Introduction
16
HPLC Pump Operating Principles
16
Isocratic and Gradient LC System Operation
16
Effects of Dissolved Oxygen on the Mobile Phase
17
Using In-Line Degassing to Remove Gases from Eluents
18
Overview of the 1500-Series HPLC Pumps
19
Fluid-Handling Components
21
Electronic Components
26
Pump Control
27
Ethernet Configuration
28
IEEE-488 Configuration
28
Options and Accessories
29
1500-Series Column Heater
29
Integral Vacuum Degasser
29
Automated Plunger Seal Wash
30
Manual Injector
30
Ethernet Communications Kit
30
Gradient Mixers
31
2 Installing the HPLC Pump
34
Site Requirements
34
Unpacking
35
Connecting Power and Signal Cables
36
Connecting the Power Supply
37
Making Ethernet Connections
37
Making IEEE-488 Connections
38
Setting the IEEE-488 Address
41
Connecting Pump Inlet and Outlet Lines
43
Connecting the Eluent Supply
43
Connecting the Pump Outlet
46
Connecting Fluid Waste Lines
48
3 Installing Options and Accessories
51
Installing the 1500-Series Column Heater
52
Installing a Manual Injector
54
Connecting to the Column or Column Heater
55
Connecting the Inject Start Signal (for Manual Injector)
55
Installing Different Eluent Mixers
58
Installing the Integral Vacuum Degasser
64
Connecting Tubing to the Degasser Inlet and Outlet
65
Installing the Degasser Vent Line
66
Using the Degasser
67
Installing the Plunger Seal Wash System
67
Preparing the Instrument
68
Removing Pump Head Components
69
Installing Head Support Components (1515/1525 Pump Only)
71
Installing Head Support Components (1525EF Pump Only)
75
Installing Head Components (1525Μ Pump Only)
77
Installing the Solenoid
79
Installing Seal Wash Tubing
81
Using the Seal Wash System
83
4 Preparing for Operation
85
Startup and Initial Preparation
86
Powering-On the Pump
86
Pump Preparation Recommendations
86
Dry Priming the Pump
88
Operating with the Integral Vacuum Degasser
89
Operating with the Plunger Seal Wash System
92
Maximum Flow Rates for 1500-Series Pumps
94
Preparing for Breeze 2 Operation
94
Priming and Purging the Pump Via Breeze 2 Control
95
Purging the Flow Path Via Breeze 2 Control
96
Equilibrating the System Via Breeze 2 Control
97
Preparing for Empower 2 Operation
99
Priming and Purging the Pump Via Empower 2 Control
99
Purging the Flow Path Via Empower 2 Control
100
Equilibrating the System Via Empower 2 Control
102
Preparing for Masslynx Operation
103
Priming and Purging the Pump Via Masslynx Control
103
Purging the Flow Path Via Masslynx Control
104
Equilibrating the System Via Masslynx Control
106
Powering off the Pump
107
5 Maintaining the HPLC Pump
109
Maintenance Considerations
110
Safety and Handling
110
Proper Operating Procedures
110
Spare Parts
110
Contacting Waters Technical Service
110
Performing Pump Diagnostic Tests
111
Retention Time Stability Test
111
Ramp-And-Decay Test
111
Replacing and Cleaning Plunger Seals and Plungers
114
Preparing for Plunger Seal Replacement
114
Cleaning and Replacing the Plungers
117
Replacing Check Valves
122
Replacing 1515/1525 Check Valves
123
Replacing 1525EF Check Valves
125
Replacing 1525Μ Check Valves
127
Replacing a Draw-Off Valve
130
Removing the Draw-Off Valve
131
Installing a Draw-Off Valve
132
Replacing Fuses
133
Replacing the Rear Panel Fuses
134
6 Troubleshooting
135
Troubleshooting Pump Problems
136
Identifying and Correcting Noises
142
Identifying Chromatographic Problems
143
A Safety Advisories
148
Warning Symbols
148
Task-Specific Hazard Warnings
148
Specific Warnings
149
Caution Symbol
151
Warnings that Apply to All Waters Instruments
152
Electrical and Handling Symbols
158
Electrical Symbols
158
Handling Symbols
159
B Specifications
161
C Solvent Considerations
169
Introduction
170
Clean Solvents
170
Solvent Quality
170
Preparation Checklist
170
Water
170
Buffers
171
Tetrahydrofuran (THF
171
Solvent Compatibility
171
Solvents to Avoid
171
Solvents to Use
171
Solvent Miscibility
174
How to Use Miscibility Numbers (M-Numbers
176
Buffered Solvents
176
Head Height
177
Solvent Viscosity
177
Mobile Phase Solvent Degassing
177
Gas Solubility
177
Eluent Degassing Methods
178
Wavelength Selection
180
UV Cutoffs for Common Solvents
180
Mixed Mobile Phases
181
Refractive Indices of Common Solvents
182
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