Equilibrating The System Via Masslynx Control - Waters 1500-Series Operator's Manual

Equilibrating the system via MassLynx control

Equilibrate the system to achieve a final state of system readiness for running
samples (see
Before equilibrating
• Make sure the eluent is filtered and degassed.
• Allow the eluent and the system to reach proper temperatures before
pump operation.
• Prime the pump, as described in the section above.
• Ensure the pump's reference valve knob is turned to the left, directing
the eluent flow to the system (for the 1525µ pump, position the vent
valve knob to Vent).
To equilibrate the system:
1. Determine flow rate and gradient settings appropriate for your column
and application, and then follow
2. Allow the system to equilibrate for several minutes.
The amount of running time required to equilibrate the system
Tip:
depends on total system volume, eluent miscibility, flow rate, and so on.
3. Monitor the system pressure and detector baseline to verify they are
normal, and inspect the eluent flow exiting the system to verify it is
normal and consistent for the specified flow rate.
4. Click the Start/Stop Pumping icon
stop the flow.
For information about creating and using an equilibration method, refer to the
MassLynx online Help.
4-22 Preparing for Operation
page 4-2).
step 1 through step 6
in the Inlet Method window to
on page 4-19.