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ThermoFisher Scientific QuantStudio 5 Manuals
Manuals and User Guides for ThermoFisher Scientific QuantStudio 5. We have
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ThermoFisher Scientific QuantStudio 5 manuals available for free PDF download: Directions For Installation, Use And Maintenance, User Manual
ThermoFisher Scientific QuantStudio 5 Directions For Installation, Use And Maintenance (113 pages)
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 2.42 MB
Table of Contents
Table of Contents
3
About this Guide
8
Revision History
8
Purpose
9
CHAPTER 1 Product Information
10
Instrument Hardware Description
10
Instrument Overview
10
Parts of the Instrument
13
Software Description
14
Instrument, Desktop, and Cloud Software Features
14
Folders, Templates, Experiments, and Projects
15
Third-Party Software Installation
16
Network Connection Options
16
Experiment Types
17
Installation and Instrument Verification
19
CHAPTER 2 Start, Sign On, and Configure the Instrument
20
Precautions for Use
20
Power on the Instrument
20
Parts of the Home Screen
21
Use the Instrument Without Signing in
23
Create a New Instrument Profile
23
Sign in
23
Sign out
24
Link Your Instrument Profile to Your Thermo Fisher Cloud Account
24
Link Your Instrument Profile to Thermo Fisher Cloud
24
Create an Instrument Profile and Link to Thermo Fisher Cloud
24
Configure Instrument Settings
25
Workflow
28
CHAPTER 3 Create and Run Experiments on the Instrument
29
Options for Running an Experiment
29
Create and Run an Experiment from a Template
29
Run an Experiment from a Saved File
29
Repeat Your Last Experiment Run
30
Edit an Experiment before Starting a Run
31
Define Experiment Properties
31
Edit a Method
32
Define Plate Wells
35
Load and Unload a Plate in the Instrument
36
View, Pause, or Stop a Run
37
View Well Details
37
Pause or Stop a Run
37
Adjust the Graphical View of an Experiment
37
Lock the Touchscreen During a Run
38
Transfer, View, or Manage Files and Results
38
Transfer Experiment Results
38
View Run History
38
Manage Template (.Edt) Files
39
CHAPTER 4 Calibrate and Verify Instrument Performance
40
Calibration and Verification Schedule
40
Calibration Descriptions
41
View the Calibration Status and Set Reminders
41
View Calibration Status and Set Reminders in the Instrument
41
View Calibration Status and Set Reminders in the Cloud
42
Perform Roi/Uniformity, Background, and Dye Calibrations
42
Workflow: Calibration
42
Prepare a Calibration Plate
43
Perform Calibrations
44
View Calibration Images and Transfer Results to USB
46
Troubleshoot Calibration Failure
47
Identify Contamination
47
(Optional)
48
Perform Instrument Verification Using Rnase P Plates
49
Instrument Verification Description and Schedule
49
Rnase P Instrument Verification Plates
49
Analytical Performance
50
Installation Specification
50
Prepare an Rnase P Plate
50
Perform Rnase P Verification
51
Troubleshoot Verification Failure
53
Calibrate Custom Dyes
54
Custom Dyes Overview
54
Dilute the Custom Dye to an Optimal Concentration
54
Calibrate the Custom Dye
57
Calibrate for a Custom Melt Curve Experiment
60
CHAPTER 5 Maintain the Instrument
62
Backup or Restore the Instrument
62
Decontaminate the Sample Block
63
Materials Required
63
Clean the Sample Block
63
Solvents for Cleaning the Sample Block
65
Replace the Instrument Fuses
65
Materials Required
65
Replace the Fuses
66
Power on or Off, Store, and Move
66
Enable Sleep Mode
66
Power on the Instrument
67
Power off the Instrument
67
Prepare the Instrument to Ship, Move, or Store
67
Move the Instrument
68
Return the Instrument for Service
68
CHAPTER 6 Profile and Instrument Configuration Tasks
70
Initial Start up
70
Manage Profiles
71
Create an Administrator Profile
71
View All User Profiles (Administrator Only)
71
Manage All Instrument Profiles (Administrator Only)
71
Edit a User Profile
72
Enable SAE Mode (Administrator Only)
72
Require Sign-In (Administrator Only)
73
Enable Remote Instrument Monitoring (Administrator Only)
73
Update Instrument Software (Administrator Only)
73
Restore Factory Defaults
74
Configure the Network
74
Set up a Wired Connection
74
Set up a Wireless Connection
75
Select a Cloud Region (Administrator Only)
75
Manage the Instrument Name (Administrator Only)
75
Set the Date and Time
76
Manage the Sign out Timer (Administrator Only)
76
Workflow: Install and Connect to a Network
77
APPENDIX A Install and Connect the Instrument to a Network
78
Before You Begin
78
Unpack and Install the Instrument
78
Power on and Follow the Startup Wizard
79
Connect the Instrument and the Computer Directly or to a LAN
79
Instrument and Computer Connections
81
Download and Install the Quantstudio ™ Design and Analysis Desktop Software
82
Computer Requirements for the Desktop Software
82
Download the Desktop Software
82
Install the Software
82
Download and Install the Quantstudio
82
Design and Analysis
82
Desktop Software
82
Networking
84
Supported Network Configuration Options
84
Control and Monitor Networked Instruments
85
About the Ethernet Port
85
Firewall Ports that Must be Open
85
Networking Guidelines and Best Practices
85
APPENDIX B Troubleshooting
87
Kits, Consumables, and Accessories
88
96-Well (0.2-Ml) Consumables
88
APPENDIX C Parts and Materials
89
384-Well Consumables
89
96-Well Fast (0.1-Ml) Consumables
90
Quantstudio ™ 3 and 5 Systems Accessories
91
General-Use Materials and Consumables
91
APPENDIX D Instrument Specification and Layout
92
Configured System Dimensions
93
Instrument Clearances
94
Electrical Requirements
94
Environmental Requirements
95
Network Requirements
95
APPENDIX E Safety
98
Symbols on this Instrument
98
Conformity Symbols
99
Safety Alerts on this Instrument
100
Location of Safety Labels on the Instrument
100
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
101
Instrument Safety
101
General
101
Safety Information for Instruments Not Manufactured by Thermo
101
Fisher Scientific
101
Physical Injury
102
Electrical
103
Cleaning and Decontamination
103
Safety and Electromagnetic Compatibility (EMC) Standards
103
Safety Compliance
104
Emc
104
Environmental Design
105
Chemical Safety
105
Biological Hazard Safety
106
Documentation and Support
107
Related Documentation
107
Obtain Information from the Help System
108
Customer and Technical Support
108
Limited Product Warranty
109
Index
110
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ThermoFisher Scientific QuantStudio 5 User Manual (66 pages)
Real-Time PCR System
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 0.72 MB
Table of Contents
Table of Contents
3
CHAPTER 1 About the Software
7
Compatible Instruments
7
Compatible Data Files
7
CHAPTER 2 Workflow
9
CHAPTER 3 Set up a Plate File
10
Select a System Template or Existing Plate File to Set up a New Plate File
10
Confirm or Edit Run Method
12
Run Method Elements
12
Apply the Recommended Run Method for Your Master MIX
12
Edit Temperature Ramp Rate, Temperature, and Time Length for a Step
13
Add or Remove a Step
13
Add or Remove a Stage
13
Add, Edit, or Remove a Pause Cycle in a PCR Stage
14
Edit the Ramp Increment for the Melt Curve Dissociation Step
15
Enable, Edit, or Disable Auto Delta
15
Enable, Edit, or Disable Veriflex Zones
16
Confirm or Edit Filter Settings
16
Confirm or Edit Plate Setup
17
Select Plate Wells
17
Add Samples and Assign to Plate Wells
17
Add Targets or SNP Assays and Assign to Plate Wells
21
Edit Reagent Information
25
Select a Passive Reference
25
Set up the Standard Curve
26
Add Biogroups and Assign Samples
27
Edit Plate File or Data File Information
27
Review the Plate File and Save
28
Export a Plate Setup File
28
CHAPTER 4 Review and Analyze Data
29
Workflow: General Procedures to Review the Primary Analysis Results
29
Open a Data File
30
Review Results in the Amplification Plot
30
Evaluate the Overall Shape of the Curves in the Amplification Plot
30
Review the Amplification Status for each Well
31
Review or Edit Threshold Settings in the Amplification Plot
31
Review or Edit Baseline Settings in the Amplification Plot
33
Optimize Display of Negative Controls in the Amplification Plot
33
Identify and Omit Outliers from Analysis
34
Review Results in the Multicomponent Plot
35
Review Results in the Raw Data Plot
36
Review Results the Melt Curve Plot
36
Review QC Alerts in the Well Table
37
Edit Primary Analysis Settings
37
View or Edit C Settings
37
View or Edit QC Alerts Settings
38
View or Edit Melt Analysis Settings
39
Use the Analysis Settings from Another File
39
View Instrument Calibration Results
40
Review Roi/Uniformity Calibration Results
40
Review Background Calibration Results
40
Review Dye Calibration Results
41
Override the Calibration Data
41
Perform Additional Analysis
42
CHAPTER 5 Export Results
43
Export the Well Table
43
Export Plate Layout as an Excel ™ Spreadsheet
43
Export Plate Layout Image
43
Export Images of Plots
43
Export Data in the Quantstudio ™ File Format
44
Export Data in the RDML Format
44
Generate a Report
44
CHAPTER 6 General Procedures to Analyze Data in the Quality Check Tab
47
View the Post-Run Summary
47
Configure the Layout of the Quality Check Tab
47
Review Individual Well Results in the Well Table
48
Review Replicate Group Results
48
Configure General Plot Settings
48
CHAPTER 7 Manage Plate Files and Data Files
49
About System Templates and Plate Files
49
About Data Files
50
Save a Plate File or Data File
50
Restrict Editing of a Plate File or Data File
50
Add a Plate File to My Plate Files
51
Search for a Plate File or Data File
51
CHAPTER 8 Manage Analysis Modules
52
About Analysis Modules
52
Select an Analysis Module
53
CHAPTER 9 Manage Export Settings
54
About Export Settings
54
View or Edit Export Settings
54
View Files in the Export Settings Library
55
Download an Export Settings File
55
Import an Export Settings File
56
CHAPTER 10 About Data Analysis
57
About the Quantification Cycle (C Q )
57
About Melt Curve Analysis
57
Primary Analysis Settings Overview
58
Settings Overview
58
Guidelines for Manual Threshold and Baseline Settings for C Analysis
59
Melt Analysis Settings Overview
59
Plots Overview
59
Amplification Plot Overview
59
Raw Data Plot Overview
60
Multi-Component Plot Overview
60
Melt Curve Plot Overview
61
APPENDIX A Troubleshooting
62
Documentation and Support
63
Related Documentation
63
Customer and Technical Support
63
Limited Product Warranty
63
Index
64
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