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ThermoFisher Scientific QuantStudio 7 Pro Manuals
Manuals and User Guides for ThermoFisher Scientific QuantStudio 7 Pro. We have
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ThermoFisher Scientific QuantStudio 7 Pro manuals available for free PDF download: User Manual
ThermoFisher Scientific QuantStudio 7 Pro User Manual (269 pages)
Real-Time PCR System
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 6.16 MB
Table of Contents
Table of Contents
7
CHAPTER 1 Product Information
17
Network and Password Security Requirements
17
Network Configuration and Security
17
Password Security
17
Recommendations for Passwords
17
Instrument Hardware Description
18
Overview of the Instrument
18
Features of each Instrument
19
Instrument Filters and Supported Dyes
19
Overview of Data Collection
21
Veriflex ™ Zones
21
Autodelta Settings
22
Parts of the Instrument
23
Instrument Status Indicator
25
Hands-Free Features
25
Facial Authentication
25
Voice Commands
25
Indicators for the Hands-Free Features
26
Software Description
27
Parts of the Home Screen
27
Thermo Fisher
29
Instrument, Desktop Software, and Thermo Fisher
29
Overview of File Locations and Files
30
Components of the Files
33
Third-Party Software
34
Network Connection Options
34
Types of Runs
34
Standard Curve Run
34
Relative Standard Curve Run
35
Comparative C T (DDC T ) Run
35
Genotyping Run
36
Presence/Absence Run
36
Melt Curve Run
36
System Template File Names
37
System Template File Names for the 96-Well 0.2-Ml Block
37
System Template File Names for the 96-Well 0.1-Ml Block
38
System Template File Names for the 384-Well Block
40
System Template File Names for the Taqman
41
Array Card Block
41
Workflow
43
CHAPTER 2 Get Started
44
Precautions for Use
44
Installation and Instrument Verification
45
Overview of Guest Access
45
Use the Instrument as a Guest
46
Power on the Instrument
47
Sign in
48
Sign in with a PIN
48
Sign in with Facial Authentication
48
Enable or Disable the Microphone
49
Use Voice Commands
49
Sign out
50
CHAPTER 3 Create and Run Plates on the Instrument
51
Options to Run a Plate
51
Create and Run a Plate from a System Template or a Saved Plate File
51
Repeat the Last Run
53
Overview of Customized Data Export
54
(Optional) Scan a Barcode Using a Barcode Scanner
55
Load a Plate into the Instrument
55
Load a Taqman ™ Array Card into the Instrument
56
Unload a Plate or a Taqman ™ Array Card from the Instrument
56
CHAPTER 4 Edit a Plate before Starting a Run
58
Overview of a Template File with Restricted Editing
58
Options to Edit the Properties of a Plate File
59
Import a Sample Setup File
59
Import an Auto Export Setting
60
Delete an Auto Export Setting
60
Options to Edit the Method of a Plate File
60
Method Elements
61
Edit the Method
61
Add or Remove Steps
62
Add or Remove Stages
62
Add or Remove Data Collection Locations
63
Set up or Remove Veriflex ™ Zones
63
Set up or Remove the Auto Delta Feature
65
Configure Ramp Rates
66
Add or Remove a Pause
66
Options to Edit the Well Details of a Plate File
66
Options to View a Plate Layout
67
Assign or Edit a Well for a Plate
69
Assign or Edit a Well for a Taqman ™ Array Card
70
Add or Edit the Reagent Details for a Plate
70
Apply the Reagents to a Plate
71
Delete the Reagent Details
71
Add or Edit the Reagent Details for a Taqman ™ Array Card
72
Apply the Reagents to a Taqman ™ Array Card
72
Edit a Passive Reference Dye
72
Import an Assay Information File (AIF)
73
Import a Taqman ™ Assay File
73
Screens Available During a Run
74
Chapter 5 Options for a Run
75
Edit a Plate File During a Run
75
Pause an Instrument Run
76
Stop an Instrument Run
76
View the Method During a Run
77
View Real-Time Data and Plots During a Run
77
Adjust the Display of Real-Time Plots During a Run
78
View the Time Remaining During a Run
78
Lock the Touchscreen During a Run
78
Unlock the Touchscreen During a Run
79
Instrument Schedule
79
Overview of the Instrument Schedule
79
Schedule a Run
79
Cancel a Scheduled Run
79
CHAPTER 6 View and Manage Files
81
Manage Plate Files
81
Copy Plate Files
81
Delete Plate Files
82
Manage Sample Setup Files
83
Copy a Sample Setup File
83
Delete a Sample Setup File
83
Manage Data Files
83
View QC Check Status after a Run
83
View an Amplification Plot after a Run
85
Transfer a Data File Immediately after a Run
86
Transfer Data Files at a Later Time
87
View the List of Data Files on the Instrument
88
Delete Data Files from the Instrument
89
CHAPTER 7 Calibrate and Verify Instrument Performance
90
Instrument and Block Calibration
90
Calibration and Verification Schedule
91
Calibration Descriptions
92
View the Calibration Status and Set Reminders
93
View the Calibration Status on the Instrument
93
Set Calibration Frequency and Reminders on the Instrument
94
View Calibration Status and Set Reminders on the Thermo Fisher
94
Review Calibration Status
95
Export a Calibration Report
95
Perform Roi/Uniformity, Background, and Dye Calibrations for Plate Blocks
95
Calibration Workflow
96
Prepare a Calibration Plate
96
Perform Calibrations
97
View the Calibration Images
99
Transfer Calibration Results
101
Transfer Calibration Data Files
101
Troubleshoot Calibration Failure
103
Identify Contamination
103
Create a Background Plate (Optional)
104
Perform Roi/Uniformity, Background, Dye, and Normalization Calibrations for the Taqman ™ Array Card Block
105
Calibration Workflow
105
Perform Roi/Uniformity, Background, Dye, and Normalization Calibrations for the
105
Array Card Block
105
Prepare a Calibration Taqman ™ Array Card
106
Perform Calibrations
108
View the Calibration Images
109
Troubleshoot Normalization Calibration Failure
110
Perform Verification Using Rnase P Plates
111
Instrument Verification Description
111
Rnase P Instrument Verification Plate
111
Performance Specifications Pass Criteria
113
Prepare an Rnase P Plate
113
Perform Rnase P Verification
114
Troubleshoot Verification Failure
115
Perform Verification Using Rnase P with a Taqman
116
Array Card
116
Rnase P Instrument Verification Taqman ™ Array Card
116
Prepare an Rnase P Taqman ™ Array Card
117
Perform Rnase P Verification
119
Calibrate Custom Dyes
120
Overview of Custom Dyes
120
Use a Dilution Series to Determine an Optimal Custom Dye Concentration
121
Calibrate the Custom Dye
125
Calibrate for a Custom Melt Curve Run
128
Require Sign-In
131
CHAPTER 8 Manage Profiles
132
Recommended Order to Set up Profiles
132
Overview of Local Instrument Profiles and Thermo Fisher Connect Platform Accounts
132
Thermo Fisher ™ Connect Platform Roles and Functions
133
Test the Connection to the Thermo Fisher
133
Connect Platform
133
Test the Connection to the Thermo Fisher ™ Connect Platform
134
Link the Instrument to Your Thermo Fisher
135
Connect Platform Account
135
Link the Instrument to Your Thermo Fisher Connect Platform Account
135
Create a New Local Instrument Profile
136
Link a Local Profile to a Thermo Fisher
137
Connect Platform Account
137
Link a Local Profile to a Thermo Fisher Connect Platform Account
137
Unlink a Thermo Fisher ™ Connect Platform Account
138
If You Link When You Are Signed in to the Instrument
139
If You Link When You Are Not Signed in to the Instrument
140
Manage Individual Instrument Profiles
141
Create an Administrator Instrument Profile During Initial Start-Up
141
Set up Facial Authentication at the Initial Creation of an Instrument Profile
141
Add Facial Authentication to an Existing Instrument Profile
142
Change the PIN for an Instrument Profile
142
Change the PIN for a Thermo Fisher ™ Connect Platform Account
143
Manage All Instrument Profiles as an Administrator
143
Reset the PIN for a Profile
143
View All Instrument Profiles
143
Add a Profile
144
Enable or Disable Administrator Privileges
144
Delete a Profile
145
E-Signature (SAE) Administrator Console
146
Overview of the SAE Administrator Console Components
147
Overview of the Instrument Features When the SAE Functions Are Enabled
147
SAE Administrator Console Functions
148
Functions that Are Controlled on the Quantstudio ™ 7 Pro Real-Time
148
PCR Instrument
148
Functions that Are Controlled in the Quantstudio ™ Design and Analysis
149
Software V2
149
Actions that Are Audited
150
Objects that Can be Audited
150
Actions that Require an E-Signature in the Quantstudio ™ 7 Pro Real-Time
151
Design and Analysis
151
PCR Instrument
151
Software V2
151
SAE Administrator Console Roles
151
Default Permissions for the Quantstudio ™ 7 Pro Real-Time PCR Instrument
152
Default Permissions for the Quantstudio ™ Design and Analysis Software V2
154
Screens that Are Displayed on the Quantstudio
156
7 Pro Real-Time PCR Instrument
156
Profiles on the Quantstudio ™ 7 Pro Real-Time PCR Instrument When the SAE
157
Functions Are Enabled
157
System Components with the SAE Functions Enabled
157
Are Enabled
157
Compatibility between SAE-Enabled and SAE-Disabled Components
158
Enable the SAE Functions
159
Workflow
159
Application Profiles
159
Enable the SAE Functions on the Quantstudio
159
Pro Real-Time PCR Instrument
160
Administrator Console
161
Enable the SAE Functions in Quantstudio
161
Design and Analysis Software V2
161
Disable the SAE Functions
162
Disable the SAE Functions in the Quantstudio
162
Design and Analysis Software V2
162
Disable the SAE Functions on the Quantstudio ™ 7 Pro Real-Time PCR
162
Instrument (Administrator Only)
162
Sign in as a Local Administrator When SAE Functions Are Enabled
163
Sign in with SAE Enabled
164
Sign out with SAE Enabled
165
Change Your SAE User Account Password on the Quantstudio
166
PCR Instrument
166
Enter Audit Reasons and E-Signatures
168
Enter an Audit Reason on the Quantstudio
168
7 Pro Real-Time PCR Instrument
168
Enter an Audit Reason in the Quantstudio ™ Design and Analysis Software V2
169
Enter an E-Signature on the Quantstudio
170
7 Pro Real-Time PCR Instrument
170
Enter an E-Signature in the Quantstudio
171
Design and Analysis Software V2
171
View the Audit and E-Signature Records in the Applications
171
Audit Records for Actions
171
Design and Analysis
172
Software V2
172
Time PCR Instrument
172
View Audit Records for Objects for the Quantstudio
172
View E-Signature Records for a Plate File on the Quantstudio ™ 7 Pro Real
172
View E-Signature Records in the Quantstudio
173
Use the Instrument When the SAE Server Is Offline
174
E-Signature Requirements to Start a Run
175
Signing in after Automatic Screen Locking
176
SAE Error Messages and Actions
176
CHAPTER 10 Maintain the Instrument
178
Install a Block
178
Change the Block
179
Remove the Block
180
Decontaminate the Sample Block
181
Materials Required
181
Clean the Sample Block
181
Detailed Procedures for Cleaning the Sample Block
182
Replace the Instrument Fuses
183
Materials Required
183
Replace the Fuses
183
Power on or Off, Store, and Move
185
Enable and Edit Sleep Mode
185
Power on the Instrument
186
Power off the Instrument
186
Prepare the Instrument to Store, Move, or Ship
187
Move the Instrument
187
CHAPTER 11 Configure the Instrument Settings
188
View the Instrument Details
188
View the License Agreement
189
View the Privacy Policy for the Thermo Fisher
189
Configure the Instrument Settings
190
Enable or Disable Demonstration Mode
190
Edit the Instrument Name (Administrator Only)
190
Set the Date and the Time
191
Configure the Heated Cover Settings
191
Adjust the Speaker Volume
191
Configure the Network
192
Connect to a Network Drive
193
Select the Region for the Thermo Fisher
195
Connect Platform
195
Select a Disk Storage Management Mode
195
Enable and Edit Automatic Sign-Out
196
Set an Access Key for External Software to Connect to the Instrument
196
Reset the Access Key for External Software to Connect to the Instrument
196
Configure Maintenance and Service Settings
197
Request Technical Support with Smart Help
197
Request Instrument Service with Smart Help
197
Perform and View Self-Verification Tests
198
View the Instrument Log
203
Export the Instrument Log
204
View the Instrument Statistics
204
Enable or Disable Remote Monitoring
205
Update the Software
205
Repair the Software
206
Back up the Instrument
206
Restore a Backup of the Instrument
207
Upload a Certificate for a Taqman ™ Array Plate with RFID
208
Overview of an Instrument Reset (Restore Factory Default)
209
Reset the Instrument (Restore Factory Default)
209
Request Remote Support
209
Configure the Instrument for the Hands-Free Features
211
Manage the Run History
211
Manage the File Name Convention of the Data File
211
Enable or Disable the Reminder to Insert a Plate
212
Select the Language
212
View the Notifications
213
Enable and Disable Home Screen Notifications
213
Set up Email Notifications
213
Clear the Notifications
214
Manage the Settings for Data Export and Data Import
214
Overview of the Settings for Data Export and Data Import
214
Manage the Settings for Data Export
215
Manage the Settings for Data Import
216
APPENDIX A Instrument Specifications and Layout
218
Instrument Dimensions
218
Instrument and Computer Clearances
219
Configured System Dimensions
220
Light Guidelines
221
Electrical Requirements
221
Environmental Requirements
222
APPENDIX B Connect the Instrument to a Network
223
Connect the Computer to the Instrument Directly or to a LAN
223
Connect the Instrument to a Wired Network
224
Connect the Instrument to a Wireless Network
224
Instrument and Computer Connections
225
Download and Install the Desktop Software
226
Computer Requirements for the Desktop Software
226
Download the Desktop Software
226
Install the Desktop Software
227
Networking
227
Supported Options for Instrument and Computer Connections
227
Monitor Instruments
228
Networking Guidelines and Best Practices
229
Firewall Ports that Must be Open
229
Ethernet Port Overview
229
Third-Party Software
230
APPENDIX C Detailed Procedures for Preparation of a Taqman
231
Array Card
231
Guidelines for Preparation of a Card
231
Taqman ™ Array Card Diagram
232
Load the PCR Reaction MIX
233
Centrifuge the Card
233
Seal the Card
235
APPENDIX D Parts and Materials
238
Kits, Consumables, Accessories, and Reagents
238
96-Well 0.2 Ml Consumables
238
96-Well 0.1 Ml Consumables
239
384-Well Consumables
239
Taqman ™ Array Card Consumables
240
Accessories
241
APPENDIX E Safety
242
Symbols on this Instrument
242
Standard Safety Symbols
243
Additional Safety Symbols
244
Location of Safety Labels
244
Control and Connection Symbols
248
Conformity Symbols
248
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
249
Instrument Safety
249
General
249
Physical Injury
250
Electrical Safety
251
Laser Safety
252
Cleaning and Decontamination
253
Instrument Component and Accessory Disposal
253
Safety and Electromagnetic Compatibility (EMC) Standards
253
Safety Standards
254
EMC Standards
254
Environmental Design Standards
254
Radio Compliance Standards
255
RF Transceiver Specifications
257
Chemical Safety
258
Biological Hazard Safety
259
APPENDIX F Documentation and Support
260
Related Documentation
260
Customer and Technical Support
261
Limited Product Warranty
261
Index
262
Advertisement
ThermoFisher Scientific QuantStudio 7 Pro User Manual (216 pages)
Real-Time PCR Systems
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 4.74 MB
Table of Contents
Table of Contents
4
CHAPTER 1 Product Information
13
Network and Password Security Requirements
13
Network Configuration and Security
13
Password Security
13
Instrument Hardware Description
14
Overview of the Instrument
14
Features of each Instrument
15
Instrument Filters and Supported Dyes
15
Overview of Data Collection
17
Veriflex ™ Zones
17
Autodelta Settings
18
Parts of the Instrument
19
Instrument Status Indicator
21
Hands-Free Features
21
Facial Authentication
21
Voice Activation
21
Indicators for the Hands-Free Features
22
Software Description
23
Parts of the Home Screen
23
Connect and Cloud Definitions
25
Instrument, Desktop Software, and Connect Platform Features
25
Overview of File Locations and Files
26
Components of the Files
29
Third-Party Software
30
Network Connection Options
30
Types of Runs
30
Standard Curve Run
30
Relative Standard Curve Run
31
Comparative C T (DDC T ) Run
31
Genotyping Run
32
Presence/Absence Run
32
Melt Curve Run
32
System Template File Names
33
System Template File Names for the 96-Well 0.2-Ml Block
33
System Template File Names for the 96-Well 0.1-Ml Block
34
System Template File Names for the 384-Well Block
36
Workflow
38
CHAPTER 2 Get Started
39
Precautions for Use
39
Installation and Instrument Verification
40
Overview of Guest Access
40
Use the Instrument as a Guest
41
Power on the Instrument
42
Sign in
43
Sign in with a PIN
43
Sign in with Facial Authentication
43
Enable or Disable the Microphone
44
Use Voice Commands
44
Sign out
45
CHAPTER 3 Create and Run Plates on the Instrument
46
Options to Run a Plate
46
Create and Run a Plate from a System Template or a Saved Plate File
46
Repeat the Last Run
48
Overview of Customized Data Export
49
(Optional) Scan a Barcode Using a Barcode Scanner
50
Load a Plate into the Instrument
50
Unload a Plate from the Instrument
51
CHAPTER 4 Edit a Plate before Starting a Run
52
Overview of a Template File with Restricted Editing
52
Options to Edit the Properties of a Plate File
53
Import a Sample Setup
53
Import an Auto Export Setting
53
Delete an Auto Export Setting
53
Options to Edit the Method of a Plate File
54
Method Elements
55
Edit the Method
55
Add or Remove Steps
56
Add or Remove Stages
56
Add or Remove Data Collection Locations
56
Set up or Remove Veriflex ™ Zones
57
Set up or Remove the Auto Delta Feature
58
Configure Ramp Rates
59
Add or Remove a Pause
59
Options to Edit the Well Details of a Plate File
60
Options to View a Plate Layout
60
View a 384-Well Plate
61
Assign or Edit a Well
62
Add or Edit the Reagent Details
63
Apply the Reagents to a Plate
63
Delete the Reagent Details
63
Edit a Passive Reference Dye
64
Import an Assay Information File (AIF)
64
CHAPTER 5 Options for a Run
65
Screens Available During a Run
65
Edit a Plate File During a Run
66
Pause an Instrument Run
67
Stop an Instrument Run
67
View the Method During a Run
68
View Real-Time Data and Plots During a Run
68
Adjust the Display of Real-Time Plots During a Run
69
View the Time Remaining During a Run
69
Lock the Touchscreen During a Run
69
Unlock the Touchscreen During a Run
70
Instrument Schedule
70
Overview of the Instrument Schedule
70
Schedule a Run
70
Cancel a Scheduled Run
71
CHAPTER 6 View and Manage Files
72
Manage Plate Files
72
Copy Plate Files
72
Delete Plate Files
73
Manage Data Files
73
View QC Check Status after a Run
73
View an Amplification Plot after a Run
75
Transfer a Data File Immediately after a Run
76
Transfer Data Files at a Later Time
76
View the List of Data Files on the Instrument
77
Delete Data Files from the Instrument
77
CHAPTER 7 Calibrate and Verify Instrument Performance
79
Instrument and Block Calibration
79
Calibration and Verification Schedule
80
Calibration Descriptions
81
View the Calibration Status and Set Reminders
81
View the Calibration Status on the Instrument
81
Set Calibration Frequency and Reminders on the Instrument
82
View Calibration Status and Set Reminders on the Connect Platform
82
Export a Calibration Report
83
Perform Roi/Uniformity, Background, and Dye Calibrations
84
Calibration Workflow
84
Prepare a Calibration Plate
84
Perform Calibrations
86
View the Calibration Images
87
Transfer Calibration Results
89
Transfer Calibration Data Files
89
Troubleshoot Calibration Failure
89
Identify Contamination
90
Create a Background Plate (Optional)
90
Perform Verification Using Rnase P Plates
91
Instrument Verification Description
91
Rnase P Instrument Verification Plate
92
Performance Specifications Pass Criteria
93
Prepare an Rnase P Plate
93
Perform Rnase P Verification
94
Troubleshoot Verification Failure
96
Calibrate Custom Dyes
98
Overview of Custom Dyes
98
Use a Dilution Series to Determine an Optimal Custom Dye Concentration
98
Calibrate the Custom Dye
101
Calibrate for a Custom Melt Curve Run
104
CHAPTER 8 Manage Profiles
107
Require Sign-In
107
Recommended Order to Set up Profiles
108
Overview of Local Instrument Profiles and Connect Profiles
108
Connect Instrument Profile Roles and Functions
109
Test the Connection to the Connect Platform
109
Link the Instrument to Your Connect Account
111
Create a New Local Instrument Profile
112
Link a Local Profile to a Connect Profile
113
Unlink a Connect Account
114
If You Link When You Are Signed in to the Instrument
115
If You Link When You Are Not Signed in to the Instrument
116
Manage Individual Instrument Profiles
117
Create an Administrator Instrument Profile During Initial Start-Up
117
Set up Facial Authentication at the Initial Creation of an Instrument Profile
117
Add Facial Authentication to an Existing Instrument Profile
118
Change the PIN for an Instrument Profile
118
Change the PIN for a Connect Profile
119
Manage All Instrument Profiles as an Administrator
119
View All Instrument Profiles
119
Reset the PIN for a Profile
119
Add a Profile
120
Enable or Disable Administrator Privileges
120
Delete a Profile
121
CHAPTER 9 Use the Instrument with the Security, Auditing, and E-Signature (SAE) V2.0 Module
123
Overview of the Security, Auditing, and E-Signature (SAE) V2.0 Module Components
123
Overview of the Instrument Features When the SAE Module Is Enabled
123
Overview of the Security, Auditing, and E-Signature (SAE) V2.0
123
Module Components
123
Security, Auditing, and E-Signature (SAE) V2.0 Module Functions
124
Functions that Are Controlled
124
Functions that Can be Audited
125
Functions that Can be Signed
125
Security, Auditing, and E-Signature (SAE) V2.0 Module Roles
125
Dialog Boxes in the SAE Module
126
Profiles When the SAE Module Is Enabled
127
SAE-Enabled System Components
127
Compatibility between SAE-Enabled and SAE-Disabled Components
128
Enable SAE Functions
129
Workflow: Enable SAE Functions
129
Configure SAE Administrator Console Application Profiles
129
Enable SAE on the Instrument and Specify the SAE Server (Administrator Only)
130
Connect to the SAE Server
131
Enable SAE Functions in Quantstudio ™ Design and Analysis Software V2
131
Disable SAE on the Instrument (Administrator Only)
132
Sign in as a Local Administrator with SAE Enabled
133
Sign in with SAE Enabled
134
Sign out with SAE Enabled
135
Change Your SAE Account Password on the Instrument
136
Sign a Plate Setup
137
Enter an Audit Reason
138
View Signing Records for a Plate File
139
View Audit Records
140
Use the Instrument When the SAE Server Is Offline
141
E-Signature Requirements to Start a Run
141
Signing in after Automatic Screen Locking
142
SAE Error Messages and Actions
142
CHAPTER 10 Maintain the Instrument
143
Install a Block
143
Change the Block
144
Remove the Block
145
Decontaminate the Sample Block
146
Materials Required
146
Clean the Sample Block
147
Detailed Procedures for Cleaning the Sample Block
148
Replace the Instrument Fuses
149
Materials Required
149
Replace the Fuses
149
Power on or Off, Store, and Move
150
Enable and Edit Sleep Mode
150
Power on the Instrument
151
Power off the Instrument
151
Prepare the Instrument to Store, Move, or Ship
152
Move the Instrument
152
CHAPTER 11 Configure the Instrument Settings
153
View the Instrument Details
153
View the License Agreement
154
View the Privacy Policy for the Connect Cloud-Based Platform
154
Configure the Instrument Settings
155
Enable or Disable Demonstration Mode
155
Edit the Instrument Name (Administrator Only)
155
Set the Date and the Time
155
Configure the Heated Cover Settings
156
Adjust the Speaker Volume
156
Configure the Network
156
Select the Region for the Connect Platform
157
Select a Disk Storage Management Mode
158
Enable and Edit Automatic Sign-Out
158
Set an Access Key for External Software to Connect to the Instrument
159
Reset the Access Key for External Software to Connect to the Instrument
159
Configure Maintenance and Service Settings
159
Request Technical Support with Smart Help
159
Request Instrument Service with Smart Help
160
Perform a Self-Verification Test
161
View the Last Self-Verification Test Result
161
View the Instrument Log
161
Export the Instrument Log
162
View the Instrument Statistics
162
Enable or Disable Remote Monitoring
163
Update the Software
163
Repair the Software
164
Back up the Instrument
164
Restore a Backup of the Instrument
165
Overview of an Instrument Reset (Restore Factory Default)
165
Reset the Instrument (Restore Factory Default)
166
Configure the Instrument for the Hands-Free Features
166
Manage the Run History
166
Manage the File Name Convention of the Data File
167
View the Notifications
167
Enable and Disable Home Screen Notifications
168
Set up Email Notifications
168
Clear the Notifications
169
APPENDIX A Instrument Specifications and Layout
170
Instrument Dimensions
170
Instrument and Computer Clearances
171
Configured System Dimensions
172
Light Guidelines
173
Electrical Requirements
173
Environmental Requirements
174
APPENDIX B Connect the Instrument to a Network
175
Connect the Computer to the Instrument Directly or to a LAN
175
Connect the Instrument to a Wired Network
176
Connect the Instrument to a Wireless Network
177
Instrument and Computer Connections
178
Download and Install the Desktop Software
179
Computer Requirements for the Desktop Software
179
Download the Desktop Software
179
Install the Desktop Software
180
Networking
180
Supported Options for Instrument and Computer Connections
180
Control and Monitor Networked Instruments
182
Networking Guidelines and Best Practices
182
Firewall Ports that Must be Open
182
Ethernet Port Overview
183
Third-Party Software
183
APPENDIX C Parts and Materials
184
Kits, Consumables, Accessories, and Reagents
184
96-Well 0.2 Ml Consumables
184
384-Well Consumables
185
96-Well 0.1 Ml Consumables
185
Accessories
186
APPENDIX D Safety
187
Symbols on this Instrument
187
Standard Safety Symbols
188
Additional Safety Symbols
189
Location of Safety Labels
189
Control and Connection Symbols
193
Conformity Symbols
193
Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
194
Instrument Safety
194
General
194
Safety Information for Instruments Not Manufactured by Thermo
194
Fisher Scientific
194
Physical Injury
195
Electrical Safety
196
Laser Safety
197
Cleaning and Decontamination
198
Instrument Component and Accessory Disposal
198
Safety and Electromagnetic Compatibility (EMC) Standards
198
Safety Standards
199
EMC Standards
199
Environmental Design Standards
200
Radio Compliance Standards
200
RF Transceiver Specifications
202
Chemical Safety
203
Biological Hazard Safety
205
APPENDIX E Documentation and Support
206
Related Documentation
206
Customer and Technical Support
207
Limited Product Warranty
207
Index
208
ThermoFisher Scientific QuantStudio 7 Pro User Manual (66 pages)
Real-Time PCR System
Brand:
ThermoFisher Scientific
| Category:
Laboratory Equipment
| Size: 0.72 MB
Table of Contents
Table of Contents
3
CHAPTER 1 About the Software
7
Compatible Instruments
7
Compatible Data Files
7
CHAPTER 2 Workflow
9
CHAPTER 3 Set up a Plate File
10
Select a System Template or Existing Plate File to Set up a New Plate File
10
Confirm or Edit Run Method
12
Run Method Elements
12
Apply the Recommended Run Method for Your Master MIX
12
Edit Temperature Ramp Rate, Temperature, and Time Length for a Step
13
Add or Remove a Step
13
Add or Remove a Stage
13
Add, Edit, or Remove a Pause Cycle in a PCR Stage
14
Edit the Ramp Increment for the Melt Curve Dissociation Step
15
Enable, Edit, or Disable Auto Delta
15
Enable, Edit, or Disable Veriflex Zones
16
Confirm or Edit Filter Settings
16
Confirm or Edit Plate Setup
17
Select Plate Wells
17
Add Samples and Assign to Plate Wells
17
Add Targets or SNP Assays and Assign to Plate Wells
21
Edit Reagent Information
25
Select a Passive Reference
25
Set up the Standard Curve
26
Add Biogroups and Assign Samples
27
Edit Plate File or Data File Information
27
Review the Plate File and Save
28
Export a Plate Setup File
28
CHAPTER 4 Review and Analyze Data
29
Workflow: General Procedures to Review the Primary Analysis Results
29
Open a Data File
30
Review Results in the Amplification Plot
30
Evaluate the Overall Shape of the Curves in the Amplification Plot
30
Review the Amplification Status for each Well
31
Review or Edit Threshold Settings in the Amplification Plot
31
Review or Edit Baseline Settings in the Amplification Plot
33
Optimize Display of Negative Controls in the Amplification Plot
33
Identify and Omit Outliers from Analysis
34
Review Results in the Multicomponent Plot
35
Review Results in the Raw Data Plot
36
Review Results the Melt Curve Plot
36
Review QC Alerts in the Well Table
37
Edit Primary Analysis Settings
37
View or Edit C Settings
37
View or Edit QC Alerts Settings
38
View or Edit Melt Analysis Settings
39
Use the Analysis Settings from Another File
39
View Instrument Calibration Results
40
Review Roi/Uniformity Calibration Results
40
Review Background Calibration Results
40
Review Dye Calibration Results
41
Override the Calibration Data
41
Perform Additional Analysis
42
CHAPTER 5 Export Results
43
Export the Well Table
43
Export Plate Layout as an Excel ™ Spreadsheet
43
Export Plate Layout Image
43
Export Images of Plots
43
Export Data in the Quantstudio ™ File Format
44
Export Data in the RDML Format
44
Generate a Report
44
CHAPTER 6 General Procedures to Analyze Data in the Quality Check Tab
47
View the Post-Run Summary
47
Configure the Layout of the Quality Check Tab
47
Review Individual Well Results in the Well Table
48
Review Replicate Group Results
48
Configure General Plot Settings
48
CHAPTER 7 Manage Plate Files and Data Files
49
About System Templates and Plate Files
49
About Data Files
50
Save a Plate File or Data File
50
Restrict Editing of a Plate File or Data File
50
Add a Plate File to My Plate Files
51
Search for a Plate File or Data File
51
CHAPTER 8 Manage Analysis Modules
52
About Analysis Modules
52
Select an Analysis Module
53
CHAPTER 9 Manage Export Settings
54
About Export Settings
54
View or Edit Export Settings
54
View Files in the Export Settings Library
55
Download an Export Settings File
55
Import an Export Settings File
56
CHAPTER 10 About Data Analysis
57
About the Quantification Cycle (C Q )
57
About Melt Curve Analysis
57
Primary Analysis Settings Overview
58
Settings Overview
58
Guidelines for Manual Threshold and Baseline Settings for C Analysis
59
Melt Analysis Settings Overview
59
Plots Overview
59
Amplification Plot Overview
59
Raw Data Plot Overview
60
Multi-Component Plot Overview
60
Melt Curve Plot Overview
61
APPENDIX A Troubleshooting
62
Documentation and Support
63
Related Documentation
63
Customer and Technical Support
63
Limited Product Warranty
63
Index
64
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